Restriction map for pF1KE0582
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# NO CUT ENZYMES (  171 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AvaII       AviII       AvrII       BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BcnI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaWI       BseAI       
BseRI       BsiEI       BsiWI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspLU11I    
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstUI       
BstXI       BstYI       BstZ17I     CelII       CfoI        
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoRV       EcoT14I     
EcoT22I     FauI        FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        HpaII       
KasI        KpnI        KspI        MaeII       MamI        
MfeI        MflI        MluI        MroI        MslI        
MspI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacI        SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        Tth111I     
XbaI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


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# ONE CUT ENZYMES (   44 enzymes) #
###################################

AciI          307  GCGG                 CCGC(-3/-1)
AocI          657  CCTAAGG              CC^T[ACGT]AGG
AspHI         651  GTGCTC               G[AT]GC[AT]^C
BalI          356  TGGCCA               TGG^CCA
BclI           92  TGATCA               T^GATCA
BfaI          698  CTAG                 C^TAG
BmyI          651  GTGCTC               G[AGT]GC[ACT]^C
BpmI          538  CTGGAG               CTGGAG(16/14)
BsgI          549  CTGCAC               GTGCAG(16/14)
BsiHKAI       651  GTGCTC               G[AT]GC[AT]^C
BsmFI         739  GTCCC                GGGAC(10/14)
Bsp1286I      651  GTGCTC               G[AGT]GC[ACT]^C
BspHI         389  TCATGA               T^CATGA
BssKI         147  CCTGG                ^CC[ACGT]GG
BstEII        106  GGTAACC              G^GT[ACGT]ACC
BstNI         147  CCTGG                CC^[AT]GG
BstPI         106  GGTAACC              G^GT[ACGT]ACC
Bsu36I        657  CCTAAGG              CC^T[ACGT]AGG
DraII         570  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EaeI          356  TGGCCA               [CT]^GGCC[GA]
Eco81I        657  CCTAAGG              CC^T[ACGT]AGG
EcoO109I      570  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       106  GGTAACC              G^GT[ACGT]ACC
EcoRII        147  CCTGG                ^CC[AT]GG
FbaI           92  TGATCA               T^GATCA
HphI           83  TCACC                GGTGA(8/7)
MaeI          698  CTAG                 C^TAG
MluNI         356  TGGCCA               TGG^CCA
MscI          356  TGGCCA               TGG^CCA
MspA1I        311  CAGCTG               C[AC]G^C[GT]G
MvaI          147  CCTGG                CC^[AT]GG
MwoI          885  GCTCTGGAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflMI         484  CCATTCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          279  GACTC                GAGTC(4/5)
PstI           52  CTGCAG               CTGCA^G
PvuII         311  CAGCTG               CAG^CTG
RcaI          389  TCATGA               T^CATGA
SapI          285  GCTCTTC              GCTCTTC(1/4)
ScrFI         147  CCTGG                CC^[ACGT]GG
SfcI           52  CTGCAG               C^T[GA][CT]AG
TaqI          506  TCGA                 T^CGA
TthHB8I       506  TCGA                 T^CGA
Van91I        484  CCATTCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          396  CCAATCTATGTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AfaI          162  GTAC                 GT^AC
              529  GTAC                
BsaI          316  GAGACC               GGTCTC(1/5)
              662  GGTCTC              
BsaJI         146  CCCTGG               C^C[ACGT][ACGT]GG
              147  CCTGGG              
BsmAI         316  GAGAC                GTCTC(1/5)
              663  GTCTC               
BspMI         110  ACCTGC               ACCTGC(4/8)
              103  GCAGGT              
BstF5I         21  CATCC                GGATG(2/0)
              627  GGATG               
Cac8I         543  GCTGGC               GC[ACGT]^[ACGT]GC
              547  GCCTGC              
EcoRI          16  GAATTC               G^AATTC
              431  GAATTC              
FokI           21  CATCC                GGATG(9/13)
              627  GGATG               
NlaIV         151  GGTTCC               GG[ACGT]^[ACGT]CC
              493  GGCCCC              
RsaI          162  GTAC                 GT^AC
              529  GTAC                
SfaNI         628  GATGC                GCATC(5/9)
              681  GCATC               
TfiI          410  GATTC                G^A[AT]TC
              455  GAATC               
Tsp45I        597  GTCAC                ^GT[GC]AC
              796  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   29 enzymes) #
#########################################

AcsI        AluI        ApoI        BbvI        BsiYI       
BslI        BsrI        Cfr13I      DdeI        DpnII       
EarI        Fnu4HI      HaeIII      HinfI       ItaI        
Ksp632I     MaeIII      MboI        MboII       MnlI        
MseI        NdeII       NlaIII      Sau3AI      Sau96I      
Tru9I       TseI        Tsp509I     TspRI       
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