Restriction map for pF1KE0581
##################################
# NO CUT ENZYMES (  139 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AvaI        AviII       AvrII       BanI        
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BfrI        BglI        BglII       BlnI        BsaAI       
BsaBI       BsaHI       BseAI       BseRI       BsiEI       
BsiWI       BsmBI       BsmI        BsoBI       Bsp1407I    
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrGI       BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EarI        
EclXI       Eco52I      Eco81I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FseI        FspI        Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        Ksp632I     KspI        MamI        MfeI        
MluI        MroI        MseI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TfiI        Tru9I       Tsp509I     Van91I      XbaI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccII         682  CGCG                 CG^CG
Aor51HI        45  AGCGCT               AGC^GCT
ApaI          435  GGGCCC               GGGCC^C
AspI          142  GACCCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI         269  GGATCC               G^GATCC
BbsI           97  GTCTTC               GAAGAC(2/6)
BclI          608  TGATCA               T^GATCA
BfaI          155  CTAG                 C^TAG
BlpI          642  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      642  GCTCAGC              GC^T[ACGT]AGC
BpuAI          97  GTCTTC               GAAGAC(2/6)
BsaI          418  GAGACC               GGTCTC(1/5)
BsaWI         191  ACCGGA               [AT]^CCGG[AT]
BspDI         160  ATCGAT               AT^CGAT
BspMI         421  ACCTGC               ACCTGC(4/8)
BsrFI         665  ACCGGC               [GA]^CCGG[CT]
BssHII        681  GCGCGC               G^CGCGC
BstAPI        618  GCAGACGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         682  CGCG                 CG^CG
CelII         642  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        665  ACCGGC               [GA]^CCGG[CT]
ClaI          160  ATCGAT               AT^CGAT
DsaI           59  CCATGG               C^C[GA][CT]GG
Eco47III       45  AGCGCT               AGC^GCT
EcoNI         345  CCTCCAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I        59  CCATGG               C^C[AT][AT]GG
FbaI          608  TGATCA               T^GATCA
MaeI          155  CTAG                 C^TAG
MaeII         527  ACGT                 A^CGT
MboII          98  TCTTC                GAAGA(8/7)
MvnI          682  CGCG                 CG^CG
NcoI           59  CCATGG               C^CATGG
PflFI         142  GACCCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         788  AGGTCCT              [GA]G^G[AT]CC[CT]
PstI          616  CTGCAG               CTGCA^G
PvuII         327  CAGCTG               CAG^CTG
SacI          334  GAGCTC               GAGCT^C
SfcI          616  CTGCAG               C^T[GA][CT]AG
SphI          635  GCATGC               GCATG^C
StyI           59  CCATGG               C^C[AT][AT]GG
TspRI         628  CACTG                CA[GC]TG[ACGT][ACGT]^
Tth111I       142  GACCCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AlwNI         619  CAGACGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              817  CAGCCGCTG           
AspHI         181  GTGCTC               G[AT]GC[AT]^C
              334  GAGCTC              
BalI          530  TGGCCA               TGG^CCA
              581  TGGCCA              
BsiHKAI       181  GTGCTC               G[AT]GC[AT]^C
              334  GAGCTC              
BsmAI         250  GAGAC                GTCTC(1/5)
              418  GAGAC               
BsrI          629  ACTGG                ACTGG(1/-1)
              303  CCAGT               
BstXI         569  CCACCTGGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              752  CCAGCGCCCTGG        
BstYI         269  GGATCC               [GA]^GATC[CT]
              359  AGATCC              
DdeI          643  CTCAG                C^T[ACGT]AG
              727  CTGAG               
DraII         435  GGGCCCC              [GA]G^G[ACGT]CC[CT]
              788  AGGTCCT             
EaeI          530  TGGCCA               [CT]^GGCC[GA]
              581  TGGCCA              
EcoO109I      435  GGGCCCC              [GA]G^G[ACGT]CC[CT]
              788  AGGTCCT             
HaeII          45  AGCGCT               [GA]GCGC^[CT]
              754  AGCGCC              
HgaI          621  GACGC                GACGC(5/10)
              494  GCGTC               
MaeIII         17  GTGAC                ^GT[ACGT]AC
              496  GTCAC               
MflI          269  GGATCC               [GA]^GATC[CT]
              359  AGATCC              
MluNI         530  TGGCCA               TGG^CCA
              581  TGGCCA              
MscI          530  TGGCCA               TGG^CCA
              581  TGGCCA              
MspA1I        327  CAGCTG               C[AC]G^C[GT]G
              820  CCGCTG              
NspI          320  GCATGT               [GA]CATG^[CT]
              635  GCATGC              
Tsp45I         17  GTGAC                ^GT[GC]AC
              496  GTCAC               
XcmI          569  CCACCTGGCTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              662  CCAACCGGCCCCTGG     
XhoII         269  GGATCC               [GA]^GATC[CT]
              359  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   54 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaII       
BanII       BbvI        BcnI        BmyI        BpmI        
BsaJI       BsgI        BsiYI       BslI        BsmFI       
Bsp1286I    BssKI       BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DpnII       EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
MboI        MnlI        MslI        MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       TaqI        TseI        TthHB8I     
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