Restriction map for pF1KE0578
##################################
# NO CUT ENZYMES (  140 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AflII       AgeI        AhdI        AlwNI       
AocI        Aor51HI     ApaI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AviII       BalI        BanII       BbvCI       BcgI        
BfrI        BlpI        Bpu1102I    BsaWI       BseAI       
BsgI        BsiEI       BsiWI       BsmI        BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FseI        FspI        HincII      
HindII      HindIII     HpaI        KpnI        Ksp632I     
KspI        MaeIII      MfeI        MluI        MluNI       
MroI        MscI        MseI        MspA1I      MunI        
MvnI        NaeI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
SwaI        Tru9I       Tsp45I      TspRI       Tth111I     
Van91I      XbaI        XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AcsI          544  AAATTC               [GA]^AATT[CT]
AflIII        388  ACACGT               A^C[GA][CT]GT
Alw44I        241  GTGCAC               G^TGCAC
ApaLI         241  GTGCAC               G^TGCAC
ApoI          544  AAATTC               [GA]^AATT[CT]
AspHI         241  GTGCAC               G[AT]GC[AT]^C
AvaI          512  CCCGGG               C^[CT]CG[GA]G
AvaII         248  GGACC                G^G[AT]CC
AvrII         342  CCTAGG               C^CTAGG
BamHI         173  GGATCC               G^GATCC
BanI          182  GGCGCC               G^G[CT][GA]CC
BbeI          182  GGCGCC               GGCGC^C
BbrPI         389  CACGTG               CAC^GTG
BbsI           19  GTCTTC               GAAGAC(2/6)
BciVI         520  GGATAC               GTATCC(6/5)
BclI          308  TGATCA               T^GATCA
BfaI          343  CTAG                 C^TAG
BglI          200  GCCGCACCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         221  AGATCT               A^GATCT
BlnI          342  CCTAGG               C^CTAGG
BpuAI          19  GTCTTC               GAAGAC(2/6)
BsaBI         309  GATCAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          602  GAGACC               GGTCTC(1/5)
BsiHKAI       241  GTGCAC               G[AT]GC[AT]^C
BsmBI          59  CGTCTC               CGTCTC(1/5)
BsmFI         247  GGGAC                GGGAC(10/14)
BsoBI         512  CCCGGG               C^[CT]CG[GA]G
Bsp1407I      452  TGTACA               T^GTACA
BsrFI         205  ACCGGC               [GA]^CCGG[CT]
BsrGI         452  TGTACA               T^GTACA
BstF5I        315  CATCC                GGATG(2/0)
Cac8I         188  GCCAGC               GC[ACGT]^[ACGT]GC
Cfr10I        205  ACCGGC               [GA]^CCGG[CT]
DraII         247  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoO109I      247  GGGACCC              [GA]G^G[ACGT]CC[CT]
FauI          251  CCCGC                CCCGC(4/6)
FbaI          308  TGATCA               T^GATCA
FokI          315  CATCC                GGATG(9/13)
HgaI           17  GCGTC                GACGC(5/10)
KasI          182  GGCGCC               G^GCGCC
MaeI          343  CTAG                 C^TAG
MamI          309  GATCAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI          389  CACGTGAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI          182  GGCGCC               GG^CGCC
NlaIII        594  CATG                 CATG^
PmaCI         389  CACGTG               CAC^GTG
PmlI          389  CACGTG               CAC^GTG
PpuMI         247  GGGACCC              [GA]G^G[AT]CC[CT]
PstI          133  CTGCAG               CTGCA^G
SfaNI         314  GCATC                GCATC(5/9)
SfoI          182  GGCGCC               GGC^GCC
SmaI          512  CCCGGG               CCC^GGG
SspBI         452  TGTACA               T^GTACA
StuI          565  AGGCCT               AGG^CCT
TfiI          275  GAATC                G^A[AT]TC
Tsp509I       545  AATT                 ^AATT
XmaCI         512  CCCGGG               C^CCGGG
XmaI          512  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AcyI           16  GGCGTC               G[GA]^CG[CT]C
              182  GGCGCC              
BbvI          135  GCAGC                GCAGC(8/12)
              132  GCTGC               
BmyI           73  GTGCCC               G[AGT]GC[ACT]^C
              241  GTGCAC              
BpmI          217  CTGGAG               CTGGAG(16/14)
              280  CTGGAG              
BsaAI         389  CACGTG               [CT]AC^GT[GA]
              484  TACGTG              
BsaHI          16  GGCGTC               G[GA]^CG[CT]C
              182  GGCGCC              
BseRI          54  CTCCTC               GAGGAG(10/8)
              359  GAGGAG              
Bsp1286I       73  GTGCCC               G[AGT]GC[ACT]^C
              241  GTGCAC              
BsrI           35  ACTGG                ACTGG(1/-1)
              425  ACTGG               
CfoI          108  GCGC                 GCG^C
              183  GCGC                
EcoT14I        29  CCTTGG               C^C[AT][AT]GG
              342  CCTAGG              
HaeII         107  AGCGCC               [GA]GCGC^[CT]
              182  GGCGCC              
HhaI          108  GCGC                 GCG^C
              183  GCGC                
Hin1I          16  GGCGTC               G[GA]^CG[CT]C
              182  GGCGCC              
HinP1I        108  GCGC                 G^CGC
              183  GCGC                
HphI          145  TCACC                GGTGA(8/7)
              508  TCACC               
MwoI          183  GCGCCGCCAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              200  GCCGCACCGGC         
StyI           29  CCTTGG               C^C[AT][AT]GG
              342  CCTAGG              
TaqI          122  TCGA                 T^CGA
              376  TCGA                
TseI          132  GCTGC                G^C[AT]GC
              135  GCAGC               
TthHB8I       122  TCGA                 T^CGA
              376  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   39 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        BcnI        
BsaJI       BsiYI       BslI        BsmAI       BssKI       
BstNI       BstYI       Cfr13I      DdeI        DpnII       
EcoRII      Fnu4HI      HaeIII      HapII       HinfI       
HpaII       ItaI        MaeII       MboI        MboII       
MflI        MnlI        MspI        MvaI        NciI        
NdeII       NlaIV       PleI        RsaI        Sau3AI      
Sau96I      ScrFI       SfcI        XhoII       
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