Restriction map for pF1KE0578
 (Circular) (Six-base) MAPSORT of: pF1KE0578.seq  Check: 1076  from: 1  to: 3734



pF1KE0578  3734 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AccI GT'mk_AC

Cuts at:    772     772 
   Size:       3734
 
Acc65I G'GTAC_C

Cuts at:    750     750 
   Size:       3734
 
AclI AA'CG_TT

Cuts at:   3574    3574 
   Size:       3734
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    295    1559    1991    2738    3006     295 
   Size:       1264     432     747     268    1023

  Fragments arranged by size: 

                1264    1023     747     432     268
 
AflIII A'CryG_T

Cuts at:    496    1086    2190    3167     496 
   Size:        590    1104     977    1063

  Fragments arranged by size: 

                1104    1063     977     590
 
AgeI A'CCGG_T

Cuts at:   2122    2122 
   Size:       3734
 
AlwNI CAG_nnn'CTG

Cuts at:   1039    2606    1039 
   Size:       1567    2167
 
ApaLI G'TGCA_C

Cuts at:    349    2504     349 
   Size:       2155    1579
 
ApoI r'AATT_y

Cuts at:    652     738    3087    3343     652 
   Size:         86    2349     256    1043

  Fragments arranged by size: 

                2349    1043     256      86
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3734
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3734
 
AvaI C'yCGr_G

Cuts at:    620     754     620 
   Size:        134    3600
 
AvrII C'CTAG_G

Cuts at:    450     450 
   Size:       3734
 
BamHI G'GATC_C

Cuts at:    281     759     281 
   Size:        478    3256
 
BanI G'GyrC_C

Cuts at:    290     750    1164    1412    1447     290 
   Size:        460     414     248      35    2577

  Fragments arranged by size: 

                2577     460     414     248      35
 
BanII G_rGCy'C

Cuts at:    748    1778     748 
   Size:       1030    2704
 
BbeI G_GCGC'C

Cuts at:    294    1168    1416     294 
   Size:        874     248    2612

  Fragments arranged by size: 

                2612     874     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    120     120 
   Size:       3734
 
BciVI GTATCCnnnnn_n'

Cuts at:    622    1632    2393    3734     622 
   Size:       1010     761    1341     622

  Fragments arranged by size: 

                1341    1010     761     622
 
BclI T'GATC_A

Cuts at:    416    1254     416 
   Size:        838    2896
 
BglI GCCn_nnn'nGGC

Cuts at:    314     314 
   Size:       3734
 
BglII A'GATC_T

Cuts at:    329    1249    3286     329 
   Size:        920    2037     777

  Fragments arranged by size: 

                2037     920     777
 
BlpI GC'TnA_GC

Cuts at:    846     846 
   Size:       3734
 
Bme1580I G_kGCm'C

Cuts at:    185     353    1359    1452    2508     185 
   Size:        168    1006      93    1056    1411

  Fragments arranged by size: 

                1411    1056    1006     168      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    153     946    1027    1362    3506     153 
   Size:        793      81     335    2144     381

  Fragments arranged by size: 

                2144     793     381     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    490    2101     490 
   Size:       1611    2123
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    346     409     346 
   Size:         63    3671
 
Bpu10I CC'TnA_GC

Cuts at:    186     186 
   Size:       3734
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    910    1789    2281    2579     910 
   Size:        879     492     298    2065

  Fragments arranged by size: 

                2065     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    704    3438     704 
   Size:       2734    1000
 
BsaAI yAC'GTr

Cuts at:    499     594    1087     499 
   Size:         95     493    3146

  Fragments arranged by size: 

                3146     493      95
 
BsaBI GATnn'nnATC

Cuts at:      6     421    1248    3285       6 
   Size:        415     827    2037     455

  Fragments arranged by size: 

                2037     827     455     415
 
BsaHI Gr'CG_yC

Cuts at:    125     291    1165    1413    2115    3611     125 
   Size:        166     874     248     702    1496     248

  Fragments arranged by size: 

                1496     874     702     248     248     166
 
BsaWI w'CCGG_w

Cuts at:    923    1444    2122    2396    2543    2954     923 
   Size:        521     678     274     147     411    1703

  Fragments arranged by size: 

                1703     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    145     175     145 
   Size:         30    3704
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    153     482     153 
   Size:        329    3405
 
BseYI C'CCAG_C

Cuts at:   1159    1917    2494    2990    1159 
   Size:        758     577     496    1903

  Fragments arranged by size: 

                1903     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1276    1322    2530     104 
   Size:       1172      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1172      46
 
BsiHKAI G_wGCw'C

Cuts at:    353     748    1526    1716    2508     353 
   Size:        395     778     190     792    1579

  Fragments arranged by size: 

                1579     792     778     395     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    173     173 
   Size:       3734
 
Bsp1286I G_dGCh'C

Cuts at:    185     353     748    1359    1452    1526    1716    1778    2508 
   Size:        168     395     611      93      74     190      62     730

Cuts at:   2508     185 
   Size:       1411

  Fragments arranged by size: 

                1411     730     611     395     190     168      93      74
                  62
 
BspEI T'CCGG_A

Cuts at:   2954    2954 
   Size:       3734
 
BspHI T'CATG_A

Cuts at:   2910    2910 
   Size:       3734
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    784     998    1300    1681     784 
   Size:        214     302     381    2837

  Fragments arranged by size: 

                2837     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2026    2080    3564    3729      50 
   Size:       1976      54    1484     165      55

  Fragments arranged by size: 

                1976    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1646    1646 
   Size:       3734
 
BsrFI r'CCGG_y

Cuts at:    313    1732    1913    2122     313 
   Size:       1419     181     209    1925

  Fragments arranged by size: 

                1925    1419     209     181
 
BsrGI T'GTAC_A

Cuts at:    560     560 
   Size:       3734
 
BssSI C'ACGA_G

Cuts at:   2005    2363    2005 
   Size:        358    3376
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1111    1111 
   Size:       3734
 
BstBI TT'CG_AA

Cuts at:   2095    2095 
   Size:       3734
 
BstYI r'GATC_y

Cuts at:      1     147     281     329     681     759    1226    1249    1584 
   Size:        146     134      48     352      78     467      23     335

Cuts at:   1584    1830    2842    2928    2940    3286       1 
   Size:        246    1012      86      12     346     449

  Fragments arranged by size: 

                1012     467     449     352     346     335     246     146
                 134      86      78      48      23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3734
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1684    1865    2028    1684 
   Size:        181     163    3390

  Fragments arranged by size: 

                3390     181     163
 
BtsI GCAGTG_nn'

Cuts at:    977     977 
   Size:       3734
 
DraI TTT'AAA

Cuts at:    733     733 
   Size:       3734
 
DrdI GACnn_nn'nnGTC

Cuts at:   1440    2298    1440 
   Size:        858    2876
 
EaeI y'GGCC_r

Cuts at:   1319    1493    1884    1911    3256    1319 
   Size:        174     391      27    1345    1797

  Fragments arranged by size: 

                1797    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1319    1319 
   Size:       3734
 
EarI CTCTTCn'nnn_

Cuts at:   1757    1967    1757 
   Size:        210    3524
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1942    2252    2398    3544    1942 
   Size:        310     146    1146    2132

  Fragments arranged by size: 

                2132    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    746     746 
   Size:       3734
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    295     346     409    1559    1991    2738    3006     295 
   Size:         51      63    1150     432     747     268    1023

  Fragments arranged by size: 

                1150    1023     747     432     268      63      51
 
EcoO109I rG'GnC_Cy

Cuts at:    356     873     356 
   Size:        517    3217
 
EcoRI G'AATT_C

Cuts at:    738     738 
   Size:       3734
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1753    1753 
   Size:       3734
 
FspI TGC'GCA

Cuts at:   1515    1515 
   Size:       3734
 
HaeII r_GCGC'y

Cuts at:    219     294    1168    1416    2438    3417     219 
   Size:         75     874     248    1022     979     536

  Fragments arranged by size: 

                1022     979     874     536     248      75
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1576    1606    1608    1638    1576 
   Size:         30       2      30    3672

  Fragments arranged by size: 

                3672      30      30       2
 
HincII GTy'rAC

Cuts at:    773     773 
   Size:       3734
 
KasI G'GCGC_C

Cuts at:    290    1164    1412     290 
   Size:        874     248    2612

  Fragments arranged by size: 

                2612     874     248
 
KpnI G_GTAC'C

Cuts at:    754     754 
   Size:       3734
 
MluI A'CGCG_T

Cuts at:   3167    3167 
   Size:       3734
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2405    2589    2405 
   Size:        184    3550
 
MscI TGG'CCA

Cuts at:   1495    1495 
   Size:       3734
 
MslI CAynn'nnrTG

Cuts at:    501    1723    1850     501 
   Size:       1222     127    2385

  Fragments arranged by size: 

                2385    1222     127
 
MspA1I CmG'CkG

Cuts at:    845    1159    1519    2532    2777     845 
   Size:        314     360    1013     245    1802

  Fragments arranged by size: 

                1802    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1915    1915 
   Size:       3734
 
NarI GG'CG_CC

Cuts at:    291    1165    1413     291 
   Size:        874     248    2612

  Fragments arranged by size: 

                2612     874     248
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3734
 
NgoMIV G'CCGG_C

Cuts at:   1913    1913 
   Size:       3734
 
NspI r_CATG'y

Cuts at:    787    1287    2194     787 
   Size:        500     907    2327

  Fragments arranged by size: 

                2327     907     500
 
PciI A'CATG_T

Cuts at:   2190    2190 
   Size:       3734
 
PfoI T'CCnGG_A

Cuts at:    386     512     386 
   Size:        126    3608
 
PmeI GTTT'AAAC

Cuts at:    733     733 
   Size:       3734
 
PmlI CAC'GTG

Cuts at:    499     499 
   Size:       3734
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2873    2905    2873 
   Size:         32    3702
 
PpuMI rG'GwC_Cy

Cuts at:    356     356 
   Size:       3734
 
PstI C_TGCA'G

Cuts at:    245     781    1466     245 
   Size:        536     685    2513

  Fragments arranged by size: 

                2513     685     536
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3734
 
PvuII CAG'CTG

Cuts at:   1159    1519    1159 
   Size:        360    3374
 
RsrII CG'GwC_CG

Cuts at:   1929    1929 
   Size:       3734
 
SacI G_AGCT'C

Cuts at:    748     748 
   Size:       3734
 
SalI G'TCGA_C

Cuts at:    771     771 
   Size:       3734
 
SanDI GG'GwC_CC

Cuts at:    356     356 
   Size:       3734
 
SapI GCTCTTCn'nnn_

Cuts at:   1757    1967    1757 
   Size:        210    3524
 
SbfI CC_TGCA'GG

Cuts at:    781     781 
   Size:       3734
 
ScaI AGT'ACT

Cuts at:   3251    3251 
   Size:       3734
 
SfcI C'TryA_G

Cuts at:     33     241     677     723     777    1462    2455    2646      33 
   Size:        208     436      46      54     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     436     208     191      54      46
            
 
SfoI GGC'GCC

Cuts at:    292    1166    1414     292 
   Size:        874     248    2612

  Fragments arranged by size: 

                2612     874     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3734
 
SmaI CCC'GGG

Cuts at:    622     756     622 
   Size:        134    3600
 
SmlI C'TyrA_G

Cuts at:    889    1804    2296    2558     889 
   Size:        915     492     262    2065

  Fragments arranged by size: 

                2065     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3734
 
SphI G_CATG'C

Cuts at:    787    1287     787 
   Size:        500    3234
 
StuI AGG'CCT

Cuts at:    675     675 
   Size:       3734
 
StyI C'CwwG_G

Cuts at:    107     137     450     868    1222     107 
   Size:         30     313     418     354    2619

  Fragments arranged by size: 

                2619     418     354     313      30
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    270    1317    1450    1909    2118     270 
   Size:       1047     133     459     209    1886

  Fragments arranged by size: 

                1886    1047     459     209     133
 
TatI w'GTAC_w

Cuts at:    560    3249     560 
   Size:       2689    1045
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    145     177    3251    3283     145 
   Size:         32    3074      32     596

  Fragments arranged by size: 

                3074     596      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1531    1531 
   Size:       3734
 
XbaI T'CTAG_A

Cuts at:    765     765 
   Size:       3734
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    114    1229     114 
   Size:       1115    2619
 
XmaI C'CCGG_G

Cuts at:    620     754     620 
   Size:        134    3600
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI    BanII
     BbeI     BbsI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NspI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI
     PstI     PvuI    PvuII    RsrII     SacI     SalI    SanDI     SapI
     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI
     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI     BaeI    BbvCI     BcgI    BfrBI    BmgBI     BmtI
     BsgI    BsiWI     BsmI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I
     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII
     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII
    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI     SwaI     XhoI
     XmnI     ZraI
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