Restriction map for pF1KE0578
 (Linear) (Six-base) MAPSORT of: KIEE0578.seq  Check: 1282  from: 1  to: 621



KIEE0578.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     621 
   Size:        187     434
 
AflIII A'CryG_T

Cuts at:      0     388     621 
   Size:        388     233
 
ApaLI G'TGCA_C

Cuts at:      0     241     621 
   Size:        241     380
 
ApoI r'AATT_y

Cuts at:      0     544     621 
   Size:        544      77
 
AvaI C'yCGr_G

Cuts at:      0     512     621 
   Size:        512     109
 
AvrII C'CTAG_G

Cuts at:      0     342     621 
   Size:        342     279
 
BamHI G'GATC_C

Cuts at:      0     173     621 
   Size:        173     448
 
BanI G'GyrC_C

Cuts at:      0     182     621 
   Size:        182     439
 
BbeI G_GCGC'C

Cuts at:      0     186     621 
   Size:        186     435
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      12     621 
   Size:         12     609
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     514     621 
   Size:        514     107
 
BclI T'GATC_A

Cuts at:      0     308     621 
   Size:        308     313
 
BglI GCCn_nnn'nGGC

Cuts at:      0     206     621 
   Size:        206     415
 
BglII A'GATC_T

Cuts at:      0     221     621 
   Size:        221     400
 
Bme1580I G_kGCm'C

Cuts at:      0      77     245     621 
   Size:         77     168     376

  Fragments arranged by size: 

                 376     168      77
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      45     621 
   Size:         45     576
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     382     621 
   Size:        382     239
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     238     301     621 
   Size:        238      63     320

  Fragments arranged by size: 

                 320     238      63
 
Bpu10I CC'TnA_GC

Cuts at:      0      78     621 
   Size:         78     543
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     596     621 
   Size:        596      25
 
BsaAI yAC'GTr

Cuts at:      0     391     486     621 
   Size:        391      95     135

  Fragments arranged by size: 

                 391     135      95
 
BsaBI GATnn'nnATC

Cuts at:      0     313     621 
   Size:        313     308
 
BsaHI Gr'CG_yC

Cuts at:      0      17     183     621 
   Size:         17     166     438

  Fragments arranged by size: 

                 438     166      17
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      37      67     621 
   Size:         37      30     554

  Fragments arranged by size: 

                 554      37      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      45     374     621 
   Size:         45     329     247

  Fragments arranged by size: 

                 329     247      45
 
BsiHKAI G_wGCw'C

Cuts at:      0     245     621 
   Size:        245     376
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      65     621 
   Size:         65     556
 
Bsp1286I G_dGCh'C

Cuts at:      0      77     245     621 
   Size:         77     168     376

  Fragments arranged by size: 

                 376     168      77
 
BsrFI r'CCGG_y

Cuts at:      0     205     621 
   Size:        205     416
 
BsrGI T'GTAC_A

Cuts at:      0     452     621 
   Size:        452     169
 
BstYI r'GATC_y

Cuts at:      0      39     173     221     573     621 
   Size:         39     134      48     352      48

  Fragments arranged by size: 

                 352     134      48      48      39
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     238     301     621 
   Size:        187      51      63     320

  Fragments arranged by size: 

                 320     187      63      51
 
EcoO109I rG'GnC_Cy

Cuts at:      0     248     621 
   Size:        248     373
 
HaeII r_GCGC'y

Cuts at:      0     111     186     621 
   Size:        111      75     435

  Fragments arranged by size: 

                 435     111      75
 
KasI G'GCGC_C

Cuts at:      0     182     621 
   Size:        182     439
 
MslI CAynn'nnrTG

Cuts at:      0     393     621 
   Size:        393     228
 
NarI GG'CG_CC

Cuts at:      0     183     621 
   Size:        183     438
 
PfoI T'CCnGG_A

Cuts at:      0     278     404     621 
   Size:        278     126     217

  Fragments arranged by size: 

                 278     217     126
 
PmlI CAC'GTG

Cuts at:      0     391     621 
   Size:        391     230
 
PpuMI rG'GwC_Cy

Cuts at:      0     248     621 
   Size:        248     373
 
PstI C_TGCA'G

Cuts at:      0     137     621 
   Size:        137     484
 
SanDI GG'GwC_CC

Cuts at:      0     248     621 
   Size:        248     373
 
SfcI C'TryA_G

Cuts at:      0     133     569     615     621 
   Size:        133     436      46       6

  Fragments arranged by size: 

                 436     133      46       6
 
SfoI GGC'GCC

Cuts at:      0     184     621 
   Size:        184     437
 
SmaI CCC'GGG

Cuts at:      0     514     621 
   Size:        514     107
 
StuI AGG'CCT

Cuts at:      0     567     621 
   Size:        567      54
 
StyI C'CwwG_G

Cuts at:      0      29     342     621 
   Size:         29     313     279

  Fragments arranged by size: 

                 313     279      29
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     162     621 
   Size:        162     459
 
TatI w'GTAC_w

Cuts at:      0     452     621 
   Size:        452     169
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      37      69     621 
   Size:         37      32     552

  Fragments arranged by size: 

                 552      37      32
 
XmaI C'CCGG_G

Cuts at:      0     512     621 
   Size:        512     109
 

 Enzymes that do cut:

     AcuI   AflIII    ApaLI     ApoI     AvaI    AvrII    BamHI     BanI
     BbeI     BbsI    BciVI     BclI     BglI    BglII Bme1580I     BmrI
     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaXI
    BseRI  BsiHKAI    BsmBI Bsp1286I    BsrFI    BsrGI    BstYI  Eco57MI
 EcoO109I    HaeII     KasI     MslI     NarI     PfoI     PmlI    PpuMI
     PstI    SanDI     SfcI     SfoI     SmaI     StuI     StyI    TaqII
     TatI     TstI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     AscI     AseI
    AsiSI     BaeI    BanII    BbvCI     BcgI    BfrBI     BlpI    BmgBI
     BmtI    BpuEI    BsaWI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BspEI    BspHI    BspMI    BsrBI    BsrDI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PmeI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI
    SexAI     SfiI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI  Tth111I     XbaI     XcmI     XhoI     XmnI
     ZraI
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