Restriction map for pF1KE0577
##################################
# NO CUT ENZYMES (  159 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AviII       
BalI        BamHI       BanII       BbrPI       BbvCI       
BcgI        BciVI       BclI        BcnI        BfrI        
BglI        BglII       BpmI        BsaAI       BsaBI       
BsaI        BsaWI       BseAI       BseRI       BsiEI       
BsiWI       BsmBI       BsmFI       BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrFI       BsrGI       BssHII      Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
EaeI        EagI        Eam1105I    EclXI       Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
FauI        FbaI        FseI        FspI        HapII       
HincII      HindII      HindIII     HpaI        HpaII       
KpnI        KspI        MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MspA1I      
MspI        MunI        MvnI        NaeI        NciI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        StuI        SwaI        TfiI        
Tth111I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AcyI          730  GGCGCC               G[GA]^CG[CT]C
Aor51HI       427  AGCGCT               AGC^GCT
AspHI         667  GTGCTC               G[AT]GC[AT]^C
AvaII         577  GGTCC                G^G[AT]CC
AvrII         241  CCTAGG               C^CTAGG
BanI          730  GGCGCC               G^G[CT][GA]CC
BbeI          730  GGCGCC               GGCGC^C
BbsI          808  GAAGAC               GAAGAC(2/6)
BbvI           72  GCTGC                GCAGC(8/12)
BlnI          241  CCTAGG               C^CTAGG
BlpI          800  GCTTAGC              GC^T[ACGT]AGC
BmyI          667  GTGCTC               G[AGT]GC[ACT]^C
Bpu1102I      800  GCTTAGC              GC^T[ACGT]AGC
BpuAI         808  GAAGAC               GAAGAC(2/6)
BsaHI         730  GGCGCC               G[GA]^CG[CT]C
BsgI           73  CTGCAC               GTGCAG(16/14)
BsiHKAI       667  GTGCTC               G[AT]GC[AT]^C
BsiYI         790  CCTGCATCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          790  CCTGCATCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         748  GTCTC                GTCTC(1/5)
BsmI          337  GAATGC               GAATGC(1/-1)
Bsp1286I      667  GTGCTC               G[AGT]GC[ACT]^C
BspMI         512  GCAGGT               ACCTGC(4/8)
BssKI         348  CCTGG                ^CC[ACGT]GG
BssSI         662  CTCGTG               CACGAG(-5/-1)
BstNI         348  CCTGG                CC^[AT]GG
Cac8I         900  GCATGC               GC[ACGT]^[ACGT]GC
CelII         800  GCTTAGC              GC^T[ACGT]AGC
Cfr13I        577  GGTCC                G^G[ACGT]CC
Eco47III      427  AGCGCT               AGC^GCT
EcoRII        348  CCTGG                ^CC[AT]GG
EcoRV         279  GATATC               GAT^ATC
HaeIII        360  GGCC                 GG^CC
HgaI          786  GCGTC                GACGC(5/10)
Hin1I         730  GGCGCC               G[GA]^CG[CT]C
HinfI          24  GACTC                G^A[ACGT]TC
KasI          730  GGCGCC               G^GCGCC
MaeII          77  ACGT                 A^CGT
MaeIII         31  GTGAC                ^GT[ACGT]AC
MvaI          348  CCTGG                CC^[AT]GG
MwoI          319  GCTTCCAGTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI          730  GGCGCC               GG^CGCC
NlaIV         730  GGCGCC               GG[ACGT]^[ACGT]CC
PleI           24  GACTC                GAGTC(4/5)
PstI          510  CTGCAG               CTGCA^G
Sau96I        577  GGTCC                G^G[ACGT]CC
ScrFI         348  CCTGG                CC^[ACGT]GG
SfoI          730  GGCGCC               GGC^GCC
SmlI          879  CTTGAG               C^T[CT][GA]AG
SphI          900  GCATGC               GCATG^C
TaqI           63  TCGA                 T^CGA
TseI           72  GCTGC                G^C[AT]GC
Tsp45I         31  GTGAC                ^GT[GC]AC
TthHB8I        63  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AciI          369  CCGC                 CCGC(-3/-1)
              728  GCGG                
AcsI          545  AAATTT               [GA]^AATT[CT]
              563  AAATTT              
ApoI          545  AAATTT               [GA]^AATT[CT]
              563  AAATTT              
BfaI          242  CTAG                 C^TAG
              286  CTAG                
BsrDI         274  GCAATG               GCAATG(2/0)
              572  GCAATG              
BstF5I        469  CATCC                GGATG(2/0)
              245  GGATG               
CfoI          428  GCGC                 GCG^C
              731  GCGC                
DpnII          61  GATC                 ^GATC
              771  GATC                
DsaI           67  CCATGG               C^C[GA][CT]GG
              119  CCATGG              
EarI           82  CTCTTC               CTCTTC(1/4)
              911  GAAGAG              
EcoT22I       898  ATGCAT               ATGCA^T
              902  ATGCAT              
Fnu4HI         72  GCTGC                GC^[ACGT]GC
              728  GCGGC               
FokI          469  CATCC                GGATG(9/13)
              245  GGATG               
HaeII         427  AGCGCT               [GA]GCGC^[CT]
              730  GGCGCC              
HhaI          428  GCGC                 GCG^C
              731  GCGC                
HinP1I        428  GCGC                 G^CGC
              731  GCGC                
ItaI           72  GCTGC                GC^[ACGT]GC
              728  GCGGC               
Ksp632I        82  CTCTTC               CTCTTC(1/4)
              911  GAAGAG              
MaeI          242  CTAG                 C^TAG
              286  CTAG                
MboI           61  GATC                 ^GATC
              771  GATC                
NcoI           67  CCATGG               C^CATGG
              119  CCATGG              
NdeII          61  GATC                 ^GATC
              771  GATC                
NsiI          898  ATGCAT               ATGCA^T
              902  ATGCAT              
NspI          724  ACATGC               [GA]CATG^[CT]
              900  GCATGC              
Sau3AI         61  GATC                 ^GATC
              771  GATC                
SfcI          332  CTACAG               C^T[GA][CT]AG
              510  CTGCAG              
TspRI         324  CAGTG                CA[GC]TG[ACGT][ACGT]^
              404  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   18 enzymes) #
#########################################

AfaI        AluI        BsaJI       BsrI        DdeI        
EcoT14I     HphI        MboII       MnlI        MseI        
MslI        NdeI        NlaIII      RsaI        SfaNI       
StyI        Tru9I       Tsp509I     
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