Restriction map for pF1KE0577
 (Linear) (Six-base) MAPSORT of: KIEE0577.seq  Check: 8547  from: 1  to: 927



KIEE0577.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     827     927 
   Size:        827     100
 
AfeI AGC'GCT

Cuts at:      0     429     927 
   Size:        429     498
 
AleI CACnn'nnGTG

Cuts at:      0     121     927 
   Size:        121     806
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     128     160     927 
   Size:        128      32     767

  Fragments arranged by size: 

                 767     128      32
 
ApoI r'AATT_y

Cuts at:      0     545     563     927 
   Size:        545      18     364

  Fragments arranged by size: 

                 545     364      18
 
AvrII C'CTAG_G

Cuts at:      0     241     927 
   Size:        241     686
 
BanI G'GyrC_C

Cuts at:      0     730     927 
   Size:        730     197
 
BbeI G_GCGC'C

Cuts at:      0     734     927 
   Size:        734     193
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     815     927 
   Size:        815     112
 
BfrBI ATG'CAT

Cuts at:      0     900     904     927 
   Size:        900       4      23

  Fragments arranged by size: 

                 900      23       4
 
BlpI GC'TnA_GC

Cuts at:      0     801     927 
   Size:        801     126
 
BmgBI CAC'GTC

Cuts at:      0      78     927 
   Size:         78     849
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     297     927 
   Size:        297     630
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     900     927 
   Size:        900      27
 
BsaHI Gr'CG_yC

Cuts at:      0     731     927 
   Size:        731     196
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     927 
   Size:         58     869
 
BsiHKAI G_wGCw'C

Cuts at:      0     671     927 
   Size:        671     256
 
BsmI GAATG_Cn'

Cuts at:      0     343     927 
   Size:        343     584
 
Bsp1286I G_dGCh'C

Cuts at:      0     671     927 
   Size:        671     256
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     503     927 
   Size:        503     424
 
BsrDI GCAATG_nn'

Cuts at:      0     281     579     927 
   Size:        281     298     348

  Fragments arranged by size: 

                 348     298     281
 
BssSI C'ACGA_G

Cuts at:      0     662     927 
   Size:        662     265
 
BtgI C'CryG_G

Cuts at:      0      67     119     927 
   Size:         67      52     808

  Fragments arranged by size: 

                 808      67      52
 
EarI CTCTTCn'nnn_

Cuts at:      0      88     906     927 
   Size:         88     818      21

  Fragments arranged by size: 

                 818      88      21
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     827     927 
   Size:        827     100
 
EcoRV GAT'ATC

Cuts at:      0     281     927 
   Size:        281     646
 
HaeII r_GCGC'y

Cuts at:      0     431     734     927 
   Size:        431     303     193

  Fragments arranged by size: 

                 431     303     193
 
KasI G'GCGC_C

Cuts at:      0     730     927 
   Size:        730     197
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     440     927 
   Size:        440     487
 
MslI CAynn'nnrTG

Cuts at:      0     118     121     637     781     927 
   Size:        118       3     516     144     146

  Fragments arranged by size: 

                 516     146     144     118       3
 
NarI GG'CG_CC

Cuts at:      0     731     927 
   Size:        731     196
 
NcoI C'CATG_G

Cuts at:      0      67     119     927 
   Size:         67      52     808

  Fragments arranged by size: 

                 808      67      52
 
NdeI CA'TA_TG

Cuts at:      0     173     382     450     927 
   Size:        173     209      68     477

  Fragments arranged by size: 

                 477     209     173      68
 
NsiI A_TGCA'T

Cuts at:      0     902     906     927 
   Size:        902       4      21

  Fragments arranged by size: 

                 902      21       4
 
NspI r_CATG'y

Cuts at:      0     728     904     927 
   Size:        728     176      23

  Fragments arranged by size: 

                 728     176      23
 
PsiI TTA'TAA

Cuts at:      0     847     919     927 
   Size:        847      72       8

  Fragments arranged by size: 

                 847      72       8
 
PstI C_TGCA'G

Cuts at:      0     514     927 
   Size:        514     413
 
SfcI C'TryA_G

Cuts at:      0     332     510     927 
   Size:        332     178     417

  Fragments arranged by size: 

                 417     332     178
 
SfoI GGC'GCC

Cuts at:      0     732     927 
   Size:        732     195
 
SmlI C'TyrA_G

Cuts at:      0     879     927 
   Size:        879      48
 
SphI G_CATG'C

Cuts at:      0     904     927 
   Size:        904      23
 
StyI C'CwwG_G

Cuts at:      0      67     119     132     192     241     927 
   Size:         67      52      13      60      49     686

  Fragments arranged by size: 

                 686      67      60      52      49      13
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     316     927 
   Size:        316     611
 
TatI w'GTAC_w

Cuts at:      0     182     220     755     927 
   Size:        182      38     535     172

  Fragments arranged by size: 

                 535     182     172      38
 

 Enzymes that do cut:

     AcuI     AfeI     AleI     AloI     ApoI    AvrII     BanI     BbeI
     BbsI    BfrBI     BlpI    BmgBI     BmrI    BpuEI    BsaHI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspMI    BsrDI    BssSI     BtgI     EarI
  Eco57MI    EcoRV    HaeII     KasI     MmeI     MslI     NarI     NcoI
     NdeI     NsiI     NspI     PsiI     PstI     SfcI     SfoI     SmlI
     SphI     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AlfI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI     BaeI    BamHI    BanII    BbvCI     BcgI    BciVI     BclI
     BglI    BglII Bme1580I     BmtI     BplI     BpmI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI    BsiEI    BsiWI
    BsmBI    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI     FalI     FseI     FspI    FspAI
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MscI
   MspA1I     NaeI   NgoMIV     NheI     NotI     NruI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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