Restriction map for pF1KE0575
##################################
# NO CUT ENZYMES (  155 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AviII       BalI        
BanI        BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BcnI        BfrI        BglI        
BlpI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsiWI       BsmBI       BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FseI        FspI        HaeII       
HapII       HgaI        Hin1I       HindIII     HpaI        
HpaII       KasI        KpnI        KspI        MamI        
MfeI        MluI        MluNI       MroI        MscI        
MseI        MspI        MunI        MvnI        NaeI        
NarI        NciI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SspBI       StuI        SwaI        Tru9I       Tth111I     
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AflIII        129  ACGTGT               A^C[GA][CT]GT
AspHI          71  GTGCTC               G[AT]GC[AT]^C
AvrII         120  CCTAGG               C^CTAGG
BamHI         515  GGATCC               G^GATCC
BanII         105  GAGCCC               G[GA]GC[CT]^C
BclI          647  TGATCA               T^GATCA
BfaI          121  CTAG                 C^TAG
BglII          53  AGATCT               A^GATCT
BlnI          120  CCTAGG               C^CTAGG
BseRI         964  CTCCTC               GAGGAG(10/8)
BsiHKAI        71  GTGCTC               G[AT]GC[AT]^C
BsmAI         810  GAGAC                GTCTC(1/5)
BspMI        1030  ACCTGC               ACCTGC(4/8)
BstF5I        503  GGATG                GGATG(2/0)
DsaI          542  CCATGG               C^C[GA][CT]GG
EaeI          100  TGGCCG               [CT]^GGCC[GA]
EarI          724  CTCTTC               CTCTTC(1/4)
FbaI          647  TGATCA               T^GATCA
FokI          503  GGATG                GGATG(9/13)
HincII        717  GTTGAC               GT[CT]^[GA]AC
HindII        717  GTTGAC               GT[CT]^[GA]AC
Ksp632I       724  CTCTTC               CTCTTC(1/4)
MaeI          121  CTAG                 C^TAG
NcoI          542  CCATGG               C^CATGG
PstI          729  CTGCAG               CTGCA^G
PvuII         314  CAGCTG               CAG^CTG
ScaI          960  AGTACT               AGT^ACT
SmlI         1012  CTCAAG               C^T[CT][GA]AG
SphI          396  GCATGC               GCATG^C
SspI          177  AATATT               AAT^ATT
TaqI          527  TCGA                 T^CGA
TthHB8I       527  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AcsI          287  GAATTT               [GA]^AATT[CT]
              448  GAATTT              
AfaI          756  GTAC                 GT^AC
              961  GTAC                
AlwI          516  GATCC                GGATC(4/5)
              515  GGATC               
AlwNI         579  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              860  CAGTCCCTG           
ApoI          287  GAATTT               [GA]^AATT[CT]
              448  GAATTT              
BsmI          480  GCATTC               GAATGC(1/-1)
              823  GCATTC              
BstXI         183  CCAAACAACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              680  CCACCCAGGTGG        
BstYI          53  AGATCT               [GA]^GATC[CT]
              515  GGATCC              
CfoI          376  GCGC                 GCG^C
              420  GCGC                
EcoT14I       120  CCTAGG               C^C[AT][AT]GG
              542  CCATGG              
HhaI          376  GCGC                 GCG^C
              420  GCGC                
HinP1I        376  GCGC                 G^CGC
              420  GCGC                
MaeII         129  ACGT                 A^CGT
              524  ACGT                
MflI           53  AGATCT               [GA]^GATC[CT]
              515  GGATCC              
MslI           64  CATGCGGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              681  CACCCAGGTG          
MspA1I        314  CAGCTG               C[AC]G^C[GT]G
              927  CCGCTG              
NdeI          168  CATATG               CA^TATG
             1035  CATATG              
NspI           63  ACATGC               [GA]CATG^[CT]
              396  GCATGC              
PflMI         498  CCAGGGGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              859  CCAGTCCCTGG         
RsaI          756  GTAC                 GT^AC
              961  GTAC                
SfaNI         581  GATGC                GCATC(5/9)
              881  GATGC               
StyI          120  CCTAGG               C^C[AT][AT]GG
              542  CCATGG              
Tsp509I       288  AATT                 ^AATT
              449  AATT                
Van91I        498  CCAGGGGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              859  CCAGTCCCTGG         
XhoII          53  AGATCT               [GA]^GATC[CT]
              515  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   46 enzymes) #
#########################################

AciI        AluI        AvaII       BbvI        BmyI        
BsaJI       BsiYI       BslI        BsmFI       Bsp1286I    
BsrI        BssKI       BstNI       Cac8I       Cfr13I      
DdeI        DpnII       DraII       EcoO109I    EcoRII      
FauI        Fnu4HI      HaeIII      HinfI       HphI        
ItaI        MaeIII      MboI        MboII       MnlI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PleI        PpuMI       Sau3AI      Sau96I      ScrFI       
SfcI        TfiI        TseI        Tsp45I      TspRI       
XcmI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com