Restriction map for pF1KE0575
 (Linear) (Six-base) MAPSORT of: KIEE0575.seq  Check: 2913  from: 1  to: 1056



KIEE0575.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     352    1056 
   Size:        352     704
 
AflIII A'CryG_T

Cuts at:      0     129    1056 
   Size:        129     927
 
AleI CACnn'nnGTG

Cuts at:      0     685    1056 
   Size:        685     371
 
AlwNI CAG_nnn'CTG

Cuts at:      0     584     865    1056 
   Size:        584     281     191

  Fragments arranged by size: 

                 584     281     191
 
ApoI r'AATT_y

Cuts at:      0     287     448    1056 
   Size:        287     161     608

  Fragments arranged by size: 

                 608     287     161
 
AvrII C'CTAG_G

Cuts at:      0     120    1056 
   Size:        120     936
 
BamHI G'GATC_C

Cuts at:      0     515    1056 
   Size:        515     541
 
BanII G_rGCy'C

Cuts at:      0     109    1056 
   Size:        109     947
 
BclI T'GATC_A

Cuts at:      0     647    1056 
   Size:        647     409
 
BglII A'GATC_T

Cuts at:      0      53    1056 
   Size:         53    1003
 
Bme1580I G_kGCm'C

Cuts at:      0     473    1056 
   Size:        473     583
 
BmgBI CAC'GTC

Cuts at:      0     130    1056 
   Size:        130     926
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     200     620     913    1056 
   Size:        200     420     293     143

  Fragments arranged by size: 

                 420     293     200     143
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     342     982    1056 
   Size:        342     640      74

  Fragments arranged by size: 

                 640     342      74
 
Bpu10I CC'TnA_GC

Cuts at:      0      74    1056 
   Size:         74     982
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     997    1056 
   Size:        997      59
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      70     100     116     146     370     395     400     425 
   Size:         70      30      16      30     224      25       5      25

Cuts at:    425    1056 
   Size:        631

  Fragments arranged by size: 

                 631     224      70      30      30      25      25      16
                   5
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     955    1056 
   Size:        955     101
 
BseYI C'CCAG_C

Cuts at:      0     485     511     705    1056 
   Size:        485      26     194     351

  Fragments arranged by size: 

                 485     351     194      26
 
BsiHKAI G_wGCw'C

Cuts at:      0      75    1056 
   Size:         75     981
 
BsmI GAATG_Cn'

Cuts at:      0     480     823    1056 
   Size:        480     343     233

  Fragments arranged by size: 

                 480     343     233
 
Bsp1286I G_dGCh'C

Cuts at:      0      75     109     473    1056 
   Size:         75      34     364     583

  Fragments arranged by size: 

                 583     364      75      34
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1039    1056 
   Size:       1039      17
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     190     687    1056 
   Size:        190     497     369

  Fragments arranged by size: 

                 497     369     190
 
BstYI r'GATC_y

Cuts at:      0      53     515    1056 
   Size:         53     462     541

  Fragments arranged by size: 

                 541     462      53
 
BtgI C'CryG_G

Cuts at:      0     542    1056 
   Size:        542     514
 
EaeI y'GGCC_r

Cuts at:      0     100    1056 
   Size:        100     956
 
EarI CTCTTCn'nnn_

Cuts at:      0     730    1056 
   Size:        730     326
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     352    1056 
   Size:        352     704
 
EcoO109I rG'GnC_Cy

Cuts at:      0     194     269     547     872    1056 
   Size:        194      75     278     325     184

  Fragments arranged by size: 

                 325     278     194     184      75
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      70     102     779     811    1056 
   Size:         70      32     677      32     245

  Fragments arranged by size: 

                 677     245      70      32      32
 
HincII GTy'rAC

Cuts at:      0     719    1056 
   Size:        719     337
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     251     264    1056 
   Size:        251      13     792

  Fragments arranged by size: 

                 792     251      13
 
MslI CAynn'nnrTG

Cuts at:      0      68     685    1056 
   Size:         68     617     371

  Fragments arranged by size: 

                 617     371      68
 
MspA1I CmG'CkG

Cuts at:      0     316     929    1056 
   Size:        316     613     127

  Fragments arranged by size: 

                 613     316     127
 
NcoI C'CATG_G

Cuts at:      0     542    1056 
   Size:        542     514
 
NdeI CA'TA_TG

Cuts at:      0     169    1036    1056 
   Size:        169     867      20

  Fragments arranged by size: 

                 867     169      20
 
NspI r_CATG'y

Cuts at:      0      67     400    1056 
   Size:         67     333     656

  Fragments arranged by size: 

                 656     333      67
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     504     865    1056 
   Size:        504     361     191

  Fragments arranged by size: 

                 504     361     191
 
PpuMI rG'GwC_Cy

Cuts at:      0     194     269     547     872    1056 
   Size:        194      75     278     325     184

  Fragments arranged by size: 

                 325     278     194     184      75
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     748     780    1056 
   Size:        748      32     276

  Fragments arranged by size: 

                 748     276      32
 
PstI C_TGCA'G

Cuts at:      0     733    1056 
   Size:        733     323
 
PvuII CAG'CTG

Cuts at:      0     316    1056 
   Size:        316     740
 
SanDI GG'GwC_CC

Cuts at:      0     194    1056 
   Size:        194     862
 
ScaI AGT'ACT

Cuts at:      0     962    1056 
   Size:        962      94
 
SfcI C'TryA_G

Cuts at:      0     273     576     729     876    1056 
   Size:        273     303     153     147     180

  Fragments arranged by size: 

                 303     273     180     153     147
 
SmlI C'TyrA_G

Cuts at:      0    1012    1056 
   Size:       1012      44
 
SphI G_CATG'C

Cuts at:      0     400    1056 
   Size:        400     656
 
SspI AAT'ATT

Cuts at:      0     179    1056 
   Size:        179     877
 
StyI C'CwwG_G

Cuts at:      0     120     542    1056 
   Size:        120     422     514

  Fragments arranged by size: 

                 514     422     120
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     395     697    1056 
   Size:        395     302     359

  Fragments arranged by size: 

                 395     359     302
 
TatI w'GTAC_w

Cuts at:      0     960    1056 
   Size:        960      96
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     116     148     370     395     402     427    1056 
   Size:        116      32     222      25       7      25     629

  Fragments arranged by size: 

                 629     222     116      32      25      25       7
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     633     702     840    1056 
   Size:        633      69     138     216

  Fragments arranged by size: 

                 633     216     138      69
 

 Enzymes that do cut:

     AcuI   AflIII     AleI    AlwNI     ApoI    AvrII    BamHI    BanII
     BclI    BglII Bme1580I    BmgBI     BmrI     BplI   Bpu10I    BpuEI
    BsaXI    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I    BspMI    BstXI
    BstYI     BtgI     EaeI     EarI  Eco57MI EcoO109I    Hin4I   HincII
     MmeI     MslI   MspA1I     NcoI     NdeI     NspI    PflMI    PpuMI
     PsrI     PstI    PvuII    SanDI     ScaI     SfcI     SmlI     SphI
     SspI     StyI    TaqII     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI     BaeI     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI
    BfrBI     BglI     BlpI     BmtI     BpmI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     NaeI     NarI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PvuI    RsrII
     SacI    SacII     SalI     SapI     SbfI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     StuI     SwaI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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