Restriction map for pF1KE0572
##################################
# NO CUT ENZYMES (  148 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BalI        BamHI       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BfrI        BglII       BlnI        
BsaAI       BsaBI       BseAI       BsiEI       BsiWI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstUI       
BstYI       BstZ17I     CfoI        Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      EcoNI       EcoRI       EcoRV       EcoT22I     
FauI        FseI        FspI        HaeII       HhaI        
HinP1I      HincII      HindII      HindIII     HpaI        
KasI        KpnI        Ksp632I     KspI        MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MslI        MunI        MvnI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        SspBI       SspI        StuI        
SwaI        Tth111I     XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AciI          306  CCGC                 CCGC(-3/-1)
AcyI           49  GACGCC               G[GA]^CG[CT]C
AfaI          701  GTAC                 GT^AC
AocI          422  CCTCAGG              CC^T[ACGT]AGG
ApaI          567  GGGCCC               GGGCC^C
AspHI         286  GAGCAC               G[AT]GC[AT]^C
AvaI           85  CTCGGG               C^[CT]CG[GA]G
BanI            3  GGCACC               G^G[CT][GA]CC
BbsI          691  GTCTTC               GAAGAC(2/6)
BclI          131  TGATCA               T^GATCA
BcnI          138  CCGGG                CC^[GC]GG
BfaI          698  CTAG                 C^TAG
BglI          323  GCCCTGCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          115  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      115  GCTGAGC              GC^T[ACGT]AGC
BpuAI         691  GTCTTC               GAAGAC(2/6)
BsaHI          49  GACGCC               G[GA]^CG[CT]C
BseRI          44  GAGGAG               GAGGAG(10/8)
BsgI           23  CTGCAC               GTGCAG(16/14)
BsiHKAI       286  GAGCAC               G[AT]GC[AT]^C
BsmBI          47  GAGACG               CGTCTC(1/5)
BsoBI          85  CTCGGG               C^[CT]CG[GA]G
BspMI         630  GCAGGT               ACCTGC(4/8)
BstEII        417  GGTGACC              G^GT[ACGT]ACC
BstPI         417  GGTGACC              G^GT[ACGT]ACC
Bsu36I        422  CCTCAGG              CC^T[ACGT]AGG
CelII         115  GCTGAGC              GC^T[ACGT]AGC
DpnII         132  GATC                 ^GATC
DsaI            7  CCATGG               C^C[GA][CT]GG
Eco81I        422  CCTCAGG              CC^T[ACGT]AGG
EcoO65I       417  GGTGACC              G^GT[ACGT]ACC
FbaI          131  TGATCA               T^GATCA
HgaI           49  GACGC                GACGC(5/10)
Hin1I          49  GACGCC               G[GA]^CG[CT]C
MaeI          698  CTAG                 C^TAG
MaeII         434  ACGT                 A^CGT
MboI          132  GATC                 ^GATC
MboII         692  TCTTC                GAAGA(8/7)
MseI          200  TTAA                 T^TAA
NciI          138  CCGGG                CC^[GC]GG
NcoI            7  CCATGG               C^CATGG
NdeII         132  GATC                 ^GATC
PflMI         104  CCACCCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          520  GACTC                GAGTC(4/5)
PpuMI         169  GGGACCC              [GA]G^G[AT]CC[CT]
RsaI          701  GTAC                 GT^AC
Sau3AI        132  GATC                 ^GATC
TaqI          467  TCGA                 T^CGA
Tru9I         200  TTAA                 T^TAA
Tsp509I       705  AATT                 ^AATT
TthHB8I       467  TCGA                 T^CGA
Van91I        104  CCACCCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AvaII         170  GGACC                G^G[AT]CC
              512  GGACC               
BsaWI         158  TCCGGT               [AT]^CCGG[AT]
              666  TCCGGT              
BsmFI         169  GGGAC                GGGAC(10/14)
              224  GGGAC               
BstAPI        621  GCATATCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              749  GCAGCCCCTGC         
BstXI         214  CCAGAACCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              407  CCAGTCCTGTGG        
Cac8I         328  GCAGGC               GC[ACGT]^[ACGT]GC
              617  GCAGGC              
EcoT14I         7  CCATGG               C^C[AT][AT]GG
              515  CCTTGG              
PvuII          28  CAGCTG               CAG^CTG
              526  CAGCTG              
SbfI          325  CCTGCAGG             CCTGCA^GG
              627  CCTGCAGG            
SfaNI         594  GATGC                GCATC(5/9)
              602  GATGC               
Sse8387I      325  CCTGCAGG             CCTGCA^GG
              627  CCTGCAGG            
StyI            7  CCATGG               C^C[AT][AT]GG
              515  CCTTGG              


#########################################
# 3 OR MORE CUT ENZYMES (   46 enzymes) #
#########################################

AluI        AlwNI       BanII       BbvI        BmyI        
BpmI        BsaI        BsaJI       BsiYI       BslI        
BsmAI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cfr13I      DdeI        DraII       EcoO109I    
EcoRII      Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        MaeIII      
MnlI        MspA1I      MspI        MvaI        MwoI        
NlaIII      NlaIV       PstI        Sau96I      ScrFI       
SfcI        TfiI        TseI        Tsp45I      TspRI       
XcmI        
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