Restriction map for pF1KE4277
##################################
# NO CUT ENZYMES (   75 enzymes) #
##################################

AatII       AclI        AflIII      AgeI        AhdI        
ApaI        AscI        AseI        AsnI        AspEI       
AspI        AviII       AvrII       BbrPI       BbvCI       
BcgI        BclI        BlnI        BsaBI       BsiWI       
Bsp1407I    BspDI       BspLU11I    BsrGI       Bst1107I    
BstBI       BstZ17I     ClaI        CpoI        DraI        
EagI        Eam1105I    EclXI       Eco52I      EcoRV       
FbaI        FseI        FspI        HpaI        MamI        
MluI        NdeI        NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SalI        SapI        
SbfI        SexAI       SfiI        SfuI        SmaI        
SpeI        Sse8387I    SspBI       SwaI        TaqI        
Tth111I     TthHB8I     XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

Acc65I        459  GGTACC               G^GTACC
AccI          482  GTAGAC               GT^[AC][GT]AC
AccIII         19  TCCGGA               T^CCGGA
AcyI          442  GGCGCC               G[GA]^CG[CT]C
AflII         837  CTTAAG               C^TTAAG
AocI          384  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      2078  AGCGCT               AGC^GCT
Asp718I       459  GGTACC               G^GTACC
BbeI          442  GGCGCC               GGCGC^C
BfrI          837  CTTAAG               C^TTAAG
BglII        2093  AGATCT               A^GATCT
BlpI          723  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      723  GCTGAGC              GC^T[ACGT]AGC
BsaAI        3104  TACGTA               [CT]AC^GT[GA]
BsaHI         442  GGCGCC               G[GA]^CG[CT]C
BsaI          674  GGTCTC               GGTCTC(1/5)
BseAI          19  TCCGGA               T^CCGGA
BsiEI        2213  CGACCG               CG[GA][CT]^CG
BspEI          19  TCCGGA               T^CCGGA
BssHII       3725  GCGCGC               G^CGCGC
BstEII        828  GGTTACC              G^GT[ACGT]ACC
BstPI         828  GGTTACC              G^GT[ACGT]ACC
Bsu36I        384  CCTGAGG              CC^T[ACGT]AGG
CelII         723  GCTGAGC              GC^T[ACGT]AGC
DrdI          103  GACGCGGTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     2078  AGCGCT               AGC^GCT
Eco81I        384  CCTGAGG              CC^T[ACGT]AGG
EcoO65I       828  GGTTACC              G^GT[ACGT]ACC
EcoT22I      2588  ATGCAT               ATGCA^T
FauI         2670  GCGGG                CCCGC(4/6)
Hin1I         442  GGCGCC               G[GA]^CG[CT]C
HincII       1192  GTCAAC               GT[CT]^[GA]AC
HindII       1192  GTCAAC               GT[CT]^[GA]AC
KasI          442  GGCGCC               G^GCGCC
KpnI          459  GGTACC               GGTAC^C
KspI           29  CCGCGG               CCGC^GG
MfeI          930  CAATTG               C^AATTG
MroI           19  TCCGGA               T^CCGGA
MslI         3120  CATGTCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI          930  CAATTG               C^AATTG
NaeI         3434  GCCGGC               GCC^GGC
NarI          442  GGCGCC               GG^CGCC
NcoI          527  CCATGG               C^CATGG
NgoMIV       3434  GCCGGC               G^CCGGC
NsiI         2588  ATGCAT               ATGCA^T
PflMI         115  CCAGTCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI         2359  CTGCAG               CTGCA^G
SacI            4  GAGCTC               GAGCT^C
SacII          29  CCGCGG               CCGC^GG
SfoI          442  GGCGCC               GGC^GCC
SgrAI         132  CGCCGGTG             C[GA]^CCGG[CT]G
SnaBI        3104  TACGTA               TAC^GTA
StuI         2813  AGGCCT               AGG^CCT
Van91I        115  CCAGTCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         3360  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

Alw44I       1450  GTGCAC               G^TGCAC
             2696  GTGCAC              
ApaLI        1450  GTGCAC               G^TGCAC
             2696  GTGCAC              
AvaI          424  CCCGAG               C^[CT]CG[GA]G
             3062  CCCGAG              
BalI         1994  TGGCCA               TGG^CCA
             2651  TGGCCA              
BamHI         152  GGATCC               G^GATCC
             1674  GGATCC              
BciVI        1242  GTATCC               GTATCC(6/5)
             1935  GTATCC              
BsaWI          19  TCCGGA               [AT]^CCGG[AT]
             2623  ACCGGA              
BsmBI        2541  CGTCTC               CGTCTC(1/5)
             2301  GAGACG              
BsmI         2035  GCATTC               GAATGC(1/-1)
             2172  GCATTC              
BsoBI         424  CCCGAG               C^[CT]CG[GA]G
             3062  CCCGAG              
BspHI        1614  TCATGA               T^CATGA
             2039  TCATGA              
BspMI        3249  GCAGGT               ACCTGC(4/8)
             3759  GCAGGT              
BsrDI        2803  GCAATG               GCAATG(2/0)
             3767  GCAATG              
DraIII       2965  CACACGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3089  CACACAGTG           
EcoNI         334  CCTGTGGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1923  CCTTATGCAGG         
EcoRI        1093  GAATTC               G^AATTC
             3820  GAATTC              
MluNI        1994  TGGCCA               TGG^CCA
             2651  TGGCCA              
MscI         1994  TGGCCA               TGG^CCA
             2651  TGGCCA              
MseI          838  TTAA                 T^TAA
             1301  TTAA                
RcaI         1614  TCATGA               T^CATGA
             2039  TCATGA              
ScaI         1465  AGTACT               AGT^ACT
             3013  AGTACT              
SfcI         2359  CTGCAG               C^T[GA][CT]AG
             2819  CTATAG              
Tru9I         838  TTAA                 T^TAA
             1301  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (  105 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        Asp700I     AspHI       
AvaII       BanI        BanII       BbsI        BbvI        
BcnI        BfaI        BglI        BmyI        BpmI        
BpuAI       BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrBI       BsrFI       BsrI        BssKI       BssSI       
BstAPI      BstF5I      BstNI       BstUI       BstXI       
BstYI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        HinP1I      HindIII     HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PleI        PpuMI       PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SmlI        SphI        
SspI        StyI        TfiI        TseI        Tsp45I      
Tsp509I     TspRI       XcmI        XhoII       XmnI        
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