Restriction map for pF1KE4277
 (Linear) (Six-base) MAPSORT of: KIEE4277.seq  Check: 6949  from: 1  to: 3840



KIEE4277.orf

 With 182 enzymes: SgfI * 

                        July 16, 2019 14:21  ..

AccI GT'mk_AC

Cuts at:      0     483    3840 
   Size:        483    3357
 
Acc65I G'GTAC_C

Cuts at:      0     459    3840 
   Size:        459    3381
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     555     840    1083    1530    2424    2734    3430    3634 
   Size:        555     285     243     447     894     310     696     204

Cuts at:   3634    3840 
   Size:        206

  Fragments arranged by size: 

                 894     696     555     447     310     285     243     206
                 204
 
AfeI AGC'GCT

Cuts at:      0    2080    3840 
   Size:       2080    1760
 
AflII C'TTAA_G

Cuts at:      0     837    3840 
   Size:        837    3003
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1824    1992    2298    3492    3836    3840 
   Size:       1824     168     306    1194     344       4

  Fragments arranged by size: 

                1824    1194     344     306     168       4
 
ApaLI G'TGCA_C

Cuts at:      0    1450    2696    3840 
   Size:       1450    1246    1144

  Fragments arranged by size: 

                1450    1246    1144
 
ApoI r'AATT_y

Cuts at:      0    1093    1351    1360    3820    3840 
   Size:       1093     258       9    2460      20

  Fragments arranged by size: 

                2460    1093     258      20       9
 
AvaI C'yCGr_G

Cuts at:      0     424    3062    3840 
   Size:        424    2638     778

  Fragments arranged by size: 

                2638     778     424
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1591    1624    3840 
   Size:       1591      33    2216

  Fragments arranged by size: 

                2216    1591      33
 
BamHI G'GATC_C

Cuts at:      0     152    1674    3840 
   Size:        152    1522    2166

  Fragments arranged by size: 

                2166    1522     152
 
BanI G'GyrC_C

Cuts at:      0     442     459    2970    3058    3840 
   Size:        442      17    2511      88     782

  Fragments arranged by size: 

                2511     782     442      88      17
 
BanII G_rGCy'C

Cuts at:      0       8     730    3208    3840 
   Size:          8     722    2478     632

  Fragments arranged by size: 

                2478     722     632       8
 
BbeI G_GCGC'C

Cuts at:      0     446    3840 
   Size:        446    3394
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2381    2885    3549    3840 
   Size:       2381     504     664     291

  Fragments arranged by size: 

                2381     664     504     291
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1253    1946    3840 
   Size:       1253     693    1894

  Fragments arranged by size: 

                1894    1253     693
 
BfrBI ATG'CAT

Cuts at:      0    2590    3840 
   Size:       2590    1250
 
BglI GCCn_nnn'nGGC

Cuts at:      0      34     587    3145    3840 
   Size:         34     553    2558     695

  Fragments arranged by size: 

                2558     695     553      34
 
BglII A'GATC_T

Cuts at:      0    2093    3840 
   Size:       2093    1747
 
BlpI GC'TnA_GC

Cuts at:      0     724    3840 
   Size:        724    3116
 
Bme1580I G_kGCm'C

Cuts at:      0    1454    2700    3063    3840 
   Size:       1454    1246     363     777

  Fragments arranged by size: 

                1454    1246     777     363
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     109    1633    1904    3026    3280    3840 
   Size:        109    1524     271    1122     254     560

  Fragments arranged by size: 

                1524    1122     560     271     254     109
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      93    2970    3840 
   Size:         93    2877     870

  Fragments arranged by size: 

                2877     870      93
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     181     475    2307    2542    2940    3180    3463 
   Size:         88      93     294    1832     235     398     240     283

Cuts at:   3463    3538    3840 
   Size:         75     302

  Fragments arranged by size: 

                1832     398     302     294     283     240     235      93
                  88      75
 
Bpu10I CC'TnA_GC

Cuts at:      0    2842    3840 
   Size:       2842     998
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1420    1573    2561    3840 
   Size:       1420     153     988    1279

  Fragments arranged by size: 

                1420    1279     988     153
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     680    3840 
   Size:        680    3160
 
BsaAI yAC'GTr

Cuts at:      0    3106    3840 
   Size:       3106     734
 
BsaHI Gr'CG_yC

Cuts at:      0     443    3840 
   Size:        443    3397
 
BsaWI w'CCGG_w

Cuts at:      0      19    2623    3840 
   Size:         19    2604    1217

  Fragments arranged by size: 

                2604    1217      19
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      25      55     154     184     262     292    3840 
   Size:         25      30      99      30      78      30    3548

  Fragments arranged by size: 

                3548      99      78      30      30      30      25
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      38     402    1444    2340    2466    3544    3840 
   Size:         38     364    1042     896     126    1078     296

  Fragments arranged by size: 

                1078    1042     896     364     296     126      38
 
BseYI C'CCAG_C

Cuts at:      0     185    1678    2724    2899    3840 
   Size:        185    1493    1046     175     941

  Fragments arranged by size: 

                1493    1046     941     185     175
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2704    3178    3490    3840 
   Size:       2704     474     312     350

  Fragments arranged by size: 

                2704     474     350     312
 
BsiEI CG_ry'CG

Cuts at:      0    2216    3840 
   Size:       2216    1624
 
BsiHKAI G_wGCw'C

Cuts at:      0       8    1454    2686    2700    3840 
   Size:          8    1446    1232      14    1140

  Fragments arranged by size: 

                1446    1232    1140      14       8
 
BsmI GAATG_Cn'

Cuts at:      0    2035    2172    3840 
   Size:       2035     137    1668

  Fragments arranged by size: 

                2035    1668     137
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2295    2547    3840 
   Size:       2295     252    1293

  Fragments arranged by size: 

                2295    1293     252
 
Bsp1286I G_dGCh'C

Cuts at:      0       8     730    1454    2686    2700    3063    3208    3840 
   Size:          8     722     724    1232      14     363     145     632

  Fragments arranged by size: 

                1232     724     722     632     363     145      14       8
            
 
BspEI T'CCGG_A

Cuts at:      0      19    3840 
   Size:         19    3821
 
BspHI T'CATG_A

Cuts at:      0    1614    2039    3840 
   Size:       1614     425    1801

  Fragments arranged by size: 

                1801    1614     425
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    3240    3750    3840 
   Size:       3240     510      90

  Fragments arranged by size: 

                3240     510      90
 
BsrBI CCG'CTC

Cuts at:      0     273    3534    3747    3840 
   Size:        273    3261     213      93

  Fragments arranged by size: 

                3261     273     213      93
 
BsrDI GCAATG_nn'

Cuts at:      0    2810    3774    3840 
   Size:       2810     964      66

  Fragments arranged by size: 

                2810     964      66
 
BsrFI r'CCGG_y

Cuts at:      0     133    3170    3434    3840 
   Size:        133    3037     264     406

  Fragments arranged by size: 

                3037     406     264     133
 
BssHII G'CGCG_C

Cuts at:      0    3725    3840 
   Size:       3725     115
 
BssSI C'ACGA_G

Cuts at:      0     633     677    1781    2240    3840 
   Size:        633      44    1104     459    1600

  Fragments arranged by size: 

                1600    1104     633     459      44
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2970    3483    3492    3840 
   Size:       2970     513       9     348

  Fragments arranged by size: 

                2970     513     348       9
 
BstEII G'GTnAC_C

Cuts at:      0     828    3840 
   Size:        828    3012
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     453     528    2743    3840 
   Size:        453      75    2215    1097

  Fragments arranged by size: 

                2215    1097     453      75
 
BstYI r'GATC_y

Cuts at:      0     152     753    1674    2093    3029    3840 
   Size:        152     601     921     419     936     811

  Fragments arranged by size: 

                 936     921     811     601     419     152
 
Bsu36I CC'TnA_GG

Cuts at:      0     385    3840 
   Size:        385    3455
 
BtgI C'CryG_G

Cuts at:      0      29     527    1830    2216    3840 
   Size:         29     498    1303     386    1624

  Fragments arranged by size: 

                1624    1303     498     386      29
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     393    3840 
   Size:        393    3447
 
BtsI GCAGTG_nn'

Cuts at:      0     533    1202    1825    2846    3840 
   Size:        533     669     623    1021     994

  Fragments arranged by size: 

                1021     994     669     623     533
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2874    2909    3840 
   Size:       2874      35     931

  Fragments arranged by size: 

                2874     931      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    2970    3094    3840 
   Size:       2970     124     746

  Fragments arranged by size: 

                2970     746     124
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     109    3840 
   Size:        109    3731
 
EaeI y'GGCC_r

Cuts at:      0      46    1994    2651    3146    3840 
   Size:         46    1948     657     495     694

  Fragments arranged by size: 

                1948     694     657     495      46
 
EarI CTCTTCn'nnn_

Cuts at:      0    1118    3195    3326    3599    3840 
   Size:       1118    2077     131     273     241

  Fragments arranged by size: 

                2077    1118     273     241     131
 
EcoICRI GAG'CTC

Cuts at:      0       6    3840 
   Size:          6    3834
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     181     475     555     840    1083    1530    2307 
   Size:         88      93     294      80     285     243     447     777

Cuts at:   2307    2424    2542    2734    2940    3180    3430    3463    3538 
   Size:        117     118     192     206     240     250      33      75

Cuts at:   3538    3634    3840 
   Size:         96     206

  Fragments arranged by size: 

                 777     447     294     285     250     243     240     206
                 206     192     118     117      96      93      88      80
                  75      33
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     338    1927    3840 
   Size:        338    1589    1913

  Fragments arranged by size: 

                1913    1589     338
 
EcoO109I rG'GnC_Cy

Cuts at:      0      96     473     981    1285    2230    2714    3840 
   Size:         96     377     508     304     945     484    1126

  Fragments arranged by size: 

                1126     945     508     484     377     304      96
 
EcoRI G'AATT_C

Cuts at:      0    1093    3820    3840 
   Size:       1093    2727      20

  Fragments arranged by size: 

                2727    1093      20
 
HaeII r_GCGC'y

Cuts at:      0      40      67     446     582    2082    3420    3840 
   Size:         40      27     379     136    1500    1338     420

  Fragments arranged by size: 

                1500    1338     420     379     136      40      27
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      25      57     476     508     658     690    1695    1727 
   Size:         25      32     419      32     150      32    1005      32

Cuts at:   1727    1936    1950    1968    1982    2085    2098    2117    2130 
   Size:        209      14      18      14     103      13      19      13

Cuts at:   2130    2575    2607    3840 
   Size:        445      32    1233

  Fragments arranged by size: 

                1233    1005     445     419     209     150     103      32
                  32      32      32      32      25      19      18      14
                  14      13      13
 
HincII GTy'rAC

Cuts at:      0    1194    3840 
   Size:       1194    2646
 
HindIII A'AGCT_T

Cuts at:      0     619    1640    3796    3840 
   Size:        619    1021    2156      44

  Fragments arranged by size: 

                2156    1021     619      44
 
KasI G'GCGC_C

Cuts at:      0     442    3840 
   Size:        442    3398
 
KpnI G_GTAC'C

Cuts at:      0     463    3840 
   Size:        463    3377
 
MfeI C'AATT_G

Cuts at:      0     930    3840 
   Size:        930    2910
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      98     125    2515    3840 
   Size:         98      27    2390    1325

  Fragments arranged by size: 

                2390    1325      98      27
 
MscI TGG'CCA

Cuts at:      0    1996    2653    3840 
   Size:       1996     657    1187

  Fragments arranged by size: 

                1996    1187     657
 
MslI CAynn'nnrTG

Cuts at:      0    3124    3840 
   Size:       3124     716
 
MspA1I CmG'CkG

Cuts at:      0      31      84     860    2667    3836    3840 
   Size:         31      53     776    1807    1169       4

  Fragments arranged by size: 

                1807    1169     776      53      31       4
 
NaeI GCC'GGC

Cuts at:      0    3436    3840 
   Size:       3436     404
 
NarI GG'CG_CC

Cuts at:      0     443    3840 
   Size:        443    3397
 
NcoI C'CATG_G

Cuts at:      0     527    3840 
   Size:        527    3313
 
NgoMIV G'CCGG_C

Cuts at:      0    3434    3840 
   Size:       3434     406
 
NsiI A_TGCA'T

Cuts at:      0    2592    3840 
   Size:       2592    1248
 
NspI r_CATG'y

Cuts at:      0     144    2590    3123    3379    3759    3840 
   Size:        144    2446     533     256     380      81

  Fragments arranged by size: 

                2446     533     380     256     144      81
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     121    3840 
   Size:        121    3719
 
PfoI T'CCnGG_A

Cuts at:      0    2558    3840 
   Size:       2558    1282
 
PpuMI rG'GwC_Cy

Cuts at:      0      96     473     981    1285    3840 
   Size:         96     377     508     304    2555

  Fragments arranged by size: 

                2555     508     377     304      96
 
PstI C_TGCA'G

Cuts at:      0    2363    3840 
   Size:       2363    1477
 
PvuII CAG'CTG

Cuts at:      0     860    2667    3836    3840 
   Size:        860    1807    1169       4

  Fragments arranged by size: 

                1807    1169     860       4
 
SacI G_AGCT'C

Cuts at:      0       8    3840 
   Size:          8    3832
 
SacII CC_GC'GG

Cuts at:      0      32    3840 
   Size:         32    3808
 
ScaI AGT'ACT

Cuts at:      0    1467    3015    3840 
   Size:       1467    1548     825

  Fragments arranged by size: 

                1548    1467     825
 
SfcI C'TryA_G

Cuts at:      0    2359    2819    3840 
   Size:       2359     460    1021

  Fragments arranged by size: 

                2359    1021     460
 
SfoI GGC'GCC

Cuts at:      0     444    3840 
   Size:        444    3396
 
SgrAI Cr'CCGG_yG

Cuts at:      0     133    3840 
   Size:        133    3707
 
SmlI C'TyrA_G

Cuts at:      0     837    1399    1588    2576    3840 
   Size:        837     562     189     988    1264

  Fragments arranged by size: 

                1264     988     837     562     189
 
SnaBI TAC'GTA

Cuts at:      0    3106    3840 
   Size:       3106     734
 
SphI G_CATG'C

Cuts at:      0    2590    3379    3759    3840 
   Size:       2590     789     380      81

  Fragments arranged by size: 

                2590     789     380      81
 
SspI AAT'ATT

Cuts at:      0    1003    1071    2369    3840 
   Size:       1003      68    1298    1471

  Fragments arranged by size: 

                1471    1298    1003      68
 
StuI AGG'CCT

Cuts at:      0    2815    3840 
   Size:       2815    1025
 
StyI C'CwwG_G

Cuts at:      0     527     804     977    1478    1976    3840 
   Size:        527     277     173     501     498    1864

  Fragments arranged by size: 

                1864     527     501     498     277     173
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     231     235    1278    2738    3840 
   Size:        231       4    1043    1460    1102

  Fragments arranged by size: 

                1460    1102    1043     231       4
 
TatI w'GTAC_w

Cuts at:      0     505     661    1252    1465    3013    3840 
   Size:        505     156     591     213    1548     827

  Fragments arranged by size: 

                1548     827     591     505     213     156
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     154     186    3840 
   Size:        154      32    3654

  Fragments arranged by size: 

                3654     154      32
 
XbaI T'CTAG_A

Cuts at:      0    3360    3840 
   Size:       3360     480
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1332    2646    3438    3840 
   Size:       1332    1314     792     402

  Fragments arranged by size: 

                1332    1314     792     402
 
XmnI GAAnn'nnTTC

Cuts at:      0     899     950    1003    3840 
   Size:        899      51      53    2837

  Fragments arranged by size: 

                2837     899      53      51
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AfeI    AflII    AlwNI    ApaLI     ApoI
     AvaI     BaeI    BamHI     BanI    BanII     BbeI     BbsI    BciVI
    BfrBI     BglI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI
   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI
   DraIII     DrdI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI     MfeI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NsiI     NspI    PflMI     PfoI    PpuMI     PstI    PvuII     SacI
    SacII     ScaI     SfcI     SfoI    SgrAI     SmlI    SnaBI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI     XbaI     XcmI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI   AflIII     AgeI     AhdI     AleI     AlfI
     AloI     ApaI     AscI     AseI    AsiSI    AvrII    BbvCI     BcgI
     BclI    BmgBI     BmtI    BsaBI    BsiWI    BsrGI    BstBI  BstZ17I
     ClaI     DraI     EagI     EciI    EcoRV     FalI     FseI     FspI
    FspAI     HpaI     MluI     NdeI     NheI     NotI     NruI     PacI
     PasI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SalI    SanDI     SapI     SbfI
    SexAI     SfiI     SgfI     SmaI     SpeI     SrfI     SwaI  Tth111I
     XhoI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com