Restriction map for pF1KE4166
##################################
# NO CUT ENZYMES (   88 enzymes) #
##################################

Acc65I      AclI        AcsI        AflII       AflIII      
AgeI        Alw44I      Aor51HI     ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspI        BcgI        BclI        BfrI        BglII       
BlpI        Bpu1102I    BsiWI       BsmI        Bsp1407I    
BspDI       BspLU11I    BsrDI       BsrGI       BssHII      
Bst1107I    BstZ17I     CelII       ClaI        CpoI        
DraI        DrdI        Eco47III    EcoRI       EcoRV       
EcoT22I     FbaI        HincII      HindII      HindIII     
HpaI        KpnI        MfeI        MluI        MseI        
MunI        NdeI        NheI        NotI        NruI        
NsiI        NspI        PacI        PaeR7I      PflFI       
PinAI       PleI        PmeI        PshAI       PshBI       
Psp1406I    PvuI        PvuII       RsrII       SalI        
SexAI       SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        SspBI       SspI        SwaI        
Tru9I       Tsp509I     Tth111I     XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccI         1519  GTCTAC               GT^[AC][GT]AC
AccIII       1118  TCCGGA               T^CCGGA
AfaI         1395  GTAC                 GT^AC
AviII          14  TGCGCA               TGC^GCA
AvrII        1137  CCTAGG               C^CTAGG
BalI         1433  TGGCCA               TGG^CCA
BamHI         290  GGATCC               G^GATCC
BbeI          964  GGCGCC               GGCGC^C
BbrPI         874  CACGTG               CAC^GTG
BbvCI        1815  CCTCAGC              CCTCAGC(-5/-2)
BciVI        1078  GGATAC               GTATCC(6/5)
BlnI         1137  CCTAGG               C^CTAGG
BsaAI         874  CACGTG               [CT]AC^GT[GA]
BsaBI         117  GATCACGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        1118  TCCGGA               [AT]^CCGG[AT]
BseAI        1118  TCCGGA               T^CCGGA
BsmBI         249  CGTCTC               CGTCTC(1/5)
BspEI        1118  TCCGGA               T^CCGGA
BspHI         401  TCATGA               T^CATGA
BsrBI        1646  CCGCTC               CCGCTC(-3/-3)
BstAPI       1504  GCACATTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         278  TTCGAA               TT^CGAA
BstEII       1101  GGTGACC              G^GT[ACGT]ACC
BstPI        1101  GGTGACC              G^GT[ACGT]ACC
BstXI        1436  CCACATCGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoO65I      1101  GGTGACC              G^GT[ACGT]ACC
FseI         1056  GGCCGGCC             GGCCGG^CC
FspI           14  TGCGCA               TGC^GCA
HinfI         276  GATTC                G^A[ACGT]TC
KasI          964  GGCGCC               G^GCGCC
MamI          117  GATCACGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        1433  TGGCCA               TGG^CCA
MroI         1118  TCCGGA               T^CCGGA
MscI         1433  TGGCCA               TGG^CCA
MslI          339  CATCCTAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI          964  GGCGCC               GG^CGCC
NspV          278  TTCGAA               TT^CGAA
PmaCI         874  CACGTG               CAC^GTG
PmlI          874  CACGTG               CAC^GTG
RcaI          401  TCATGA               T^CATGA
RsaI         1395  GTAC                 GT^AC
SbfI         1296  CCTGCAGG             CCTGCA^GG
ScaI         1394  AGTACT               AGT^ACT
SfoI          964  GGCGCC               GGC^GCC
SfuI          278  TTCGAA               TT^CGAA
Sse8387I     1296  CCTGCAGG             CCTGCA^GG
StuI         1301  AGGCCT               AGG^CCT
TfiI          276  GATTC                G^A[AT]TC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AhdI          361  GACCTGCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1489  GACCCAAAGTC         
AocI          453  CCTGAGG              CC^T[ACGT]AGG
              526  CCTGAGG             
AspEI         361  GACCTGCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1489  GACCCAAAGTC         
BbsI           48  GAAGAC               GAAGAC(2/6)
              687  GAAGAC              
BfaI          343  CTAG                 C^TAG
             1138  CTAG                
BpmI          676  CTGGAG               CTGGAG(16/14)
              838  CTGGAG              
BpuAI          48  GAAGAC               GAAGAC(2/6)
              687  GAAGAC              
BsaI          424  GAGACC               GGTCTC(1/5)
              793  GAGACC              
BstYI         212  AGATCC               [GA]^GATC[CT]
              290  GGATCC              
Bsu36I        453  CCTGAGG              CC^T[ACGT]AGG
              526  CCTGAGG             
DraIII        160  CACATCGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1505  CACATTGTG           
Eam1105I      361  GACCTGCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1489  GACCCAAAGTC         
Eco81I        453  CCTGAGG              CC^T[ACGT]AGG
              526  CCTGAGG             
KspI          128  CCGCGG               CCGC^GG
             1314  CCGCGG              
MaeI          343  CTAG                 C^TAG
             1138  CTAG                
MflI          212  AGATCC               [GA]^GATC[CT]
              290  GGATCC              
NaeI          785  GCCGGC               GCC^GGC
             1057  GCCGGC              
NcoI          380  CCATGG               C^CATGG
             1715  CCATGG              
NgoMIV        785  GCCGGC               G^CCGGC
             1057  GCCGGC              
PflMI         159  CCACATCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1530  CCATTACATGG         
PstI          888  CTGCAG               CTGCA^G
             1297  CTGCAG              
SacII         128  CCGCGG               CCGC^GG
             1314  CCGCGG              
SfiI          663  GGCCCCGCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             1797  GGCCGCCGTGGCC       
TaqI          279  TCGA                 T^CGA
             1364  TCGA                
TthHB8I       279  TCGA                 T^CGA
             1364  TCGA                
Van91I        159  CCACATCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1530  CCATTACATGG         
XhoII         212  AGATCC               [GA]^GATC[CT]
              290  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   95 enzymes) #
#########################################

AatII       AccII       AciI        AcyI        AluI        
AlwI        AlwNI       ApaI        AspHI       AvaI        
AvaII       BanI        BanII       BbvI        BcnI        
BglI        BmyI        BsaHI       BsaJI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrFI       BsrI        BssKI       BssSI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EagI        EarI        EclXI       Eco52I      
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HpaII       
HphI        ItaI        Ksp632I     MaeII       MaeIII      
MboI        MboII       MnlI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PpuMI       SacI        SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StyI        TseI        Tsp45I      TspRI       XcmI        
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