Restriction map for pF1KE4166
 (Linear) (Six-base) MAPSORT of: KIEE4166.seq  Check: 3534  from: 1  to: 1854



KIEE4166.orf

 With 182 enzymes: SgfI * 

                        July 16, 2019 14:21  ..

AatII G_ACGT'C

Cuts at:      0      55     137     251    1118    1854 
   Size:         55      82     114     867     736

  Fragments arranged by size: 

                 867     736     114      82      55
 
AccI GT'mk_AC

Cuts at:      0    1520    1854 
   Size:       1520     334
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     561    1854 
   Size:        561    1293
 
AhdI GACnn_n'nnGTC

Cuts at:      0     366    1494    1854 
   Size:        366    1128     360

  Fragments arranged by size: 

                1128     366     360
 
AlwNI CAG_nnn'CTG

Cuts at:      0     564     597     675    1854 
   Size:        564      33      78    1179

  Fragments arranged by size: 

                1179     564      78      33
 
ApaI G_GGCC'C

Cuts at:      0     326    1027    1272    1854 
   Size:        326     701     245     582

  Fragments arranged by size: 

                 701     582     326     245
 
AvaI C'yCGr_G

Cuts at:      0      88     415    1027    1854 
   Size:         88     327     612     827

  Fragments arranged by size: 

                 827     612     327      88
 
AvrII C'CTAG_G

Cuts at:      0    1137    1854 
   Size:       1137     717
 
BamHI G'GATC_C

Cuts at:      0     290    1854 
   Size:        290    1564
 
BanI G'GyrC_C

Cuts at:      0     150     330     653     964    1325    1854 
   Size:        150     180     323     311     361     529

  Fragments arranged by size: 

                 529     361     323     311     180     150
 
BanII G_rGCy'C

Cuts at:      0     326     379     626     902    1027    1034    1272    1685 
   Size:        326      53     247     276     125       7     238     413

Cuts at:   1685    1854 
   Size:        169

  Fragments arranged by size: 

                 413     326     276     247     238     169     125      53
                   7
 
BbeI G_GCGC'C

Cuts at:      0     968    1854 
   Size:        968     886
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      55     694    1854 
   Size:         55     639    1160

  Fragments arranged by size: 

                1160     639      55
 
BbvCI CC'TCA_GC

Cuts at:      0    1816    1854 
   Size:       1816      38
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1072    1854 
   Size:       1072     782
 
BglI GCCn_nnn'nGGC

Cuts at:      0     490     670    1717    1804    1854 
   Size:        490     180    1047      87      50

  Fragments arranged by size: 

                1047     490     180      87      50
 
Bme1580I G_kGCm'C

Cuts at:      0     155     326     335     656     881    1027    1272    1328 
   Size:        155     171       9     321     225     146     245      56

Cuts at:   1328    1420    1854 
   Size:         92     434

  Fragments arranged by size: 

                 434     321     245     225     171     155     146      92
                  56       9
 
BmgBI CAC'GTC

Cuts at:      0      12     684    1854 
   Size:         12     672    1170

  Fragments arranged by size: 

                1170     672      12
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      37    1854 
   Size:         37    1817
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     697     859    1854 
   Size:        697     162     995

  Fragments arranged by size: 

                 995     697     162
 
Bpu10I CC'TnA_GC

Cuts at:      0    1816    1854 
   Size:       1816      38
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     418     787    1854 
   Size:        418     369    1067

  Fragments arranged by size: 

                1067     418     369
 
BsaAI yAC'GTr

Cuts at:      0     876    1854 
   Size:        876     978
 
BsaBI GATnn'nnATC

Cuts at:      0     121    1854 
   Size:        121    1733
 
BsaHI Gr'CG_yC

Cuts at:      0      52     134     248     965    1115    1448    1854 
   Size:         52      82     114     717     150     333     406

  Fragments arranged by size: 

                 717     406     333     150     114      82      52
 
BsaWI w'CCGG_w

Cuts at:      0    1118    1854 
   Size:       1118     736
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     844     874    1854 
   Size:        844      30     980

  Fragments arranged by size: 

                 980     844      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      94     106     127     211     223     271    1557    1854 
   Size:         94      12      21      84      12      48    1286     297

  Fragments arranged by size: 

                1286     297      94      84      48      21      12      12
            
 
BseYI C'CCAG_C

Cuts at:      0    1008    1674    1684    1703    1782    1854 
   Size:       1008     666      10      19      79      72

  Fragments arranged by size: 

                1008     666      79      72      19      10
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     720     946    1057    1393    1854 
   Size:        720     226     111     336     461

  Fragments arranged by size: 

                 720     461     336     226     111
 
BsiEI CG_ry'CG

Cuts at:      0     370     786    1062    1314    1619    1854 
   Size:        370     416     276     252     305     235

  Fragments arranged by size: 

                 416     370     305     276     252     235
 
BsiHKAI G_wGCw'C

Cuts at:      0     379     395     626     902    1034    1854 
   Size:        379      16     231     276     132     820

  Fragments arranged by size: 

                 820     379     276     231     132      16
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     255    1854 
   Size:        255    1599
 
Bsp1286I G_dGCh'C

Cuts at:      0     155     326     335     379     395     626     656     881 
   Size:        155     171       9      44      16     231      30     225

Cuts at:    881     902    1027    1034    1272    1328    1420    1685    1854 
   Size:         21     125       7     238      56      92     265     169

  Fragments arranged by size: 

                 265     238     231     225     171     169     155     125
                  92      56      44      30      21      16       9       7
            
 
BspEI T'CCGG_A

Cuts at:      0    1118    1854 
   Size:       1118     736
 
BspHI T'CATG_A

Cuts at:      0     401    1854 
   Size:        401    1453
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     371     436    1365    1589    1854 
   Size:        371      65     929     224     265

  Fragments arranged by size: 

                 929     371     265     224      65
 
BsrBI CCG'CTC

Cuts at:      0    1648    1854 
   Size:       1648     206
 
BsrFI r'CCGG_y

Cuts at:      0     731     785    1057    1061    1614    1637    1854 
   Size:        731      54     272       4     553      23     217

  Fragments arranged by size: 

                 731     553     272     217      54      23       4
 
BssSI C'ACGA_G

Cuts at:      0     790    1199    1335    1854 
   Size:        790     409     136     519

  Fragments arranged by size: 

                 790     519     409     136
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1510    1854 
   Size:       1510     344
 
BstBI TT'CG_AA

Cuts at:      0     279    1854 
   Size:        279    1575
 
BstEII G'GTnAC_C

Cuts at:      0    1101    1854 
   Size:       1101     753
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1443    1854 
   Size:       1443     411
 
BstYI r'GATC_y

Cuts at:      0     212     290    1854 
   Size:        212      78    1564

  Fragments arranged by size: 

                1564     212      78
 
Bsu36I CC'TnA_GG

Cuts at:      0     454     527    1854 
   Size:        454      73    1327

  Fragments arranged by size: 

                1327     454      73
 
BtgI C'CryG_G

Cuts at:      0     128     380     977    1272    1314    1715    1802    1854 
   Size:        128     252     597     295      42     401      87      52

  Fragments arranged by size: 

                 597     401     295     252     128      87      52      42
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     845    1424    1854 
   Size:        845     579     430

  Fragments arranged by size: 

                 845     579     430
 
BtsI GCAGTG_nn'

Cuts at:      0    1491    1854 
   Size:       1491     363
 
DraIII CAC_nnn'GTG

Cuts at:      0     165    1510    1854 
   Size:        165    1345     344

  Fragments arranged by size: 

                1345     344     165
 
EaeI y'GGCC_r

Cuts at:      0     443     783    1059    1311    1433    1616    1718    1805 
   Size:        443     340     276     252     122     183     102      87

Cuts at:   1805    1854 
   Size:         49

  Fragments arranged by size: 

                 443     340     276     252     183     122     102      87
                  49
 
EagI C'GGCC_G

Cuts at:      0     783    1059    1311    1616    1854 
   Size:        783     276     252     305     238

  Fragments arranged by size: 

                 783     305     276     252     238
 
EarI CTCTTCn'nnn_

Cuts at:      0     614     760     775    1201    1764    1840    1854 
   Size:        614     146      15     426     563      76      14

  Fragments arranged by size: 

                 614     563     426     146      76      15      14
 
EcoICRI GAG'CTC

Cuts at:      0     377     624     900    1032    1854 
   Size:        377     247     276     132     822

  Fragments arranged by size: 

                 822     377     276     247     132
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     561     697     859    1854 
   Size:        561     136     162     995

  Fragments arranged by size: 

                 995     561     162     136
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     601    1255    1408    1854 
   Size:        601     654     153     446

  Fragments arranged by size: 

                 654     601     446     153
 
EcoO109I rG'GnC_Cy

Cuts at:      0      96     322     594     672    1023    1024    1268    1836 
   Size:         96     226     272      78     351       1     244     568

Cuts at:   1836    1854 
   Size:         18

  Fragments arranged by size: 

                 568     351     272     244     226      96      78      18
                   1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1308    1854 
   Size:       1308     546
 
FseI GG_CCGG'CC

Cuts at:      0    1061    1854 
   Size:       1061     793
 
FspI TGC'GCA

Cuts at:      0      16    1854 
   Size:         16    1838
 
FspAI rTGC'GCAy

Cuts at:      0      16    1854 
   Size:         16    1838
 
HaeII r_GCGC'y

Cuts at:      0     565     610     819     968    1018    1854 
   Size:        565      45     209     149      50     836

  Fragments arranged by size: 

                 836     565     209     149      50      45
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     352     384     844     876    1480    1512    1854 
   Size:        352      32     460      32     604      32     342

  Fragments arranged by size: 

                 604     460     352     342      32      32      32
 
KasI G'GCGC_C

Cuts at:      0     964    1854 
   Size:        964     890
 
MscI TGG'CCA

Cuts at:      0    1435    1854 
   Size:       1435     419
 
MslI CAynn'nnrTG

Cuts at:      0     343    1854 
   Size:        343    1511
 
MspA1I CmG'CkG

Cuts at:      0     130    1239    1316    1854 
   Size:        130    1109      77     538

  Fragments arranged by size: 

                1109     538     130      77
 
NaeI GCC'GGC

Cuts at:      0     787    1059    1854 
   Size:        787     272     795

  Fragments arranged by size: 

                 795     787     272
 
NarI GG'CG_CC

Cuts at:      0     965    1854 
   Size:        965     889
 
NcoI C'CATG_G

Cuts at:      0     380    1715    1854 
   Size:        380    1335     139

  Fragments arranged by size: 

                1335     380     139
 
NgoMIV G'CCGG_C

Cuts at:      0     785    1057    1854 
   Size:        785     272     797

  Fragments arranged by size: 

                 797     785     272
 
PasI CC'CwG_GG

Cuts at:      0     326    1019    1854 
   Size:        326     693     835

  Fragments arranged by size: 

                 835     693     326
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     165    1536    1854 
   Size:        165    1371     318

  Fragments arranged by size: 

                1371     318     165
 
PmlI CAC'GTG

Cuts at:      0     876    1854 
   Size:        876     978
 
PpuMI rG'GwC_Cy

Cuts at:      0      96     594    1836    1854 
   Size:         96     498    1242      18

  Fragments arranged by size: 

                1242     498      96      18
 
PspOMI G'GGCC_C

Cuts at:      0     322    1023    1268    1854 
   Size:        322     701     245     586

  Fragments arranged by size: 

                 701     586     322     245
 
PstI C_TGCA'G

Cuts at:      0     892    1301    1854 
   Size:        892     409     553

  Fragments arranged by size: 

                 892     553     409
 
SacI G_AGCT'C

Cuts at:      0     379     626     902    1034    1854 
   Size:        379     247     276     132     820

  Fragments arranged by size: 

                 820     379     276     247     132
 
SacII CC_GC'GG

Cuts at:      0     131    1317    1854 
   Size:        131    1186     537

  Fragments arranged by size: 

                1186     537     131
 
SapI GCTCTTCn'nnn_

Cuts at:      0     614     775    1840    1854 
   Size:        614     161    1065      14

  Fragments arranged by size: 

                1065     614     161      14
 
SbfI CC_TGCA'GG

Cuts at:      0    1301    1854 
   Size:       1301     553
 
ScaI AGT'ACT

Cuts at:      0    1396    1854 
   Size:       1396     458
 
SfcI C'TryA_G

Cuts at:      0     825     888    1297    1830    1854 
   Size:        825      63     409     533      24

  Fragments arranged by size: 

                 825     533     409      63      24
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     670    1804    1854 
   Size:        670    1134      50

  Fragments arranged by size: 

                1134     670      50
 
SfoI GGC'GCC

Cuts at:      0     966    1854 
   Size:        966     888
 
StuI AGG'CCT

Cuts at:      0    1303    1854 
   Size:       1303     551
 
StyI C'CwwG_G

Cuts at:      0     380     909    1128    1137    1571    1715    1854 
   Size:        380     529     219       9     434     144     139

  Fragments arranged by size: 

                 529     434     380     219     144     139       9
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     172    1717    1854 
   Size:        172    1545     137

  Fragments arranged by size: 

                1545     172     137
 
TatI w'GTAC_w

Cuts at:      0    1394    1854 
   Size:       1394     460
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1028    1060    1721    1753    1854 
   Size:       1028      32     661      32     101

  Fragments arranged by size: 

                1028     661     101      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     162     342    1016    1854 
   Size:        162     180     674     838

  Fragments arranged by size: 

                 838     674     180     162
 
ZraI GAC'GTC

Cuts at:      0      53     135     249    1116    1854 
   Size:         53      82     114     867     738

  Fragments arranged by size: 

                 867     738     114      82      53
 

 Enzymes that do cut:

    AatII     AccI     AcuI     AhdI    AlwNI     ApaI     AvaI    AvrII
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI    BciVI     BglI
 Bme1580I    BmgBI     BmrI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI   DraIII     EaeI     EagI     EarI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     FalI     FseI     FspI    FspAI    HaeII    Hin4I     KasI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     PasI
    PflMI     PmlI    PpuMI   PspOMI     PstI     SacI    SacII     SapI
     SbfI     ScaI     SfcI     SfiI     SfoI     StuI     StyI    TaqII
     TatI     TstI     XcmI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AleI
     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI     BaeI
     BcgI     BclI    BfrBI    BglII     BlpI     BmtI     BplI    BpuEI
    BsiWI     BsmI    BsrDI    BsrGI   BssHII  BstZ17I     ClaI    CspCI
     DraI     DrdI     EciI    EcoRI    EcoRV   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     NdeI     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI     PfoI     PmeI     PpiI    PshAI
     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII     SalI    SanDI
    SexAI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI  Tth111I     XbaI     XhoI     XmaI     XmnI
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