Restriction map for pF1KE3228
##################################
# NO CUT ENZYMES (  108 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AviII       
AvrII       BalI        BamHI       BanII       BbeI        
BbsI        BbvCI       BcgI        BciVI       BfrI        
BlnI        BlpI        Bpu1102I    BpuAI       BsaI        
BsiWI       BsmI        Bsp1407I    BspDI       BspLU11I    
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstZ17I     CelII       
ClaI        CpoI        DraI        DraIII      Eam1105I    
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FseI        FspI        HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MfeI        
MluI        MluNI       MscI        MunI        NarI        
NdeI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmeI        PshBI       
Psp1406I    PstI        PvuI        RsrII       SacI        
SacII       SalI        SbfI        SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        XbaI        
XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccIII        293  TCCGGA               T^CCGGA
AocI         1024  CCTGAGG              CC^T[ACGT]AGG
AspI         1093  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          134  CCCGGG               C^[CT]CG[GA]G
BbrPI         727  CACGTG               CAC^GTG
BglI          755  GCCGGCACGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII          35  AGATCT               A^GATCT
BsaAI         727  CACGTG               [CT]AC^GT[GA]
BsaBI         717  GATGAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         293  TCCGGA               [AT]^CCGG[AT]
BseAI         293  TCCGGA               T^CCGGA
BsmBI        1371  GAGACG               CGTCTC(1/5)
BsoBI         134  CCCGGG               C^[CT]CG[GA]G
BspEI         293  TCCGGA               T^CCGGA
BspMI         878  ACCTGC               ACCTGC(4/8)
BsrDI         300  GCAATG               GCAATG(2/0)
BsrFI         755  GCCGGC               [GA]^CCGG[CT]
BstXI         488  CCAATATCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1024  CCTGAGG              CC^T[ACGT]AGG
Cfr10I        755  GCCGGC               [GA]^CCGG[CT]
DrdI          973  GACGCGGGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1024  CCTGAGG              CC^T[ACGT]AGG
EcoT14I       548  CCATGG               C^C[AT][AT]GG
MaeIII       1384  GTCAC                ^GT[ACGT]AC
MamI          717  GATGAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI          293  TCCGGA               T^CCGGA
NaeI          755  GCCGGC               GCC^GGC
NcoI          548  CCATGG               C^CATGG
NgoMIV        755  GCCGGC               G^CCGGC
NheI         1392  GCTAGC               G^CTAGC
NspI         1443  GCATGT               [GA]CATG^[CT]
PflFI        1093  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         727  CACGTG               CAC^GTG
PmlI          727  CACGTG               CAC^GTG
PshAI        1260  GACGCCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI          903  GAAGAGC              GCTCTTC(1/4)
ScaI         1055  AGTACT               AGT^ACT
SexAI        1094  ACCTGGT              A^CC[AT]GGT
SfcI          473  CTACAG               C^T[GA][CT]AG
SmaI          134  CCCGGG               CCC^GGG
SmlI          318  CTTGAG               C^T[CT][GA]AG
StuI          807  AGGCCT               AGG^CCT
StyI          548  CCATGG               C^C[AT][AT]GG
TfiI         1288  GAATC                G^A[AT]TC
Tsp45I       1384  GTCAC                ^GT[GC]AC
Tsp509I       360  AATT                 ^AATT
Tth111I      1093  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI         134  CCCGGG               C^CCGGG
XmaI          134  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

Aor51HI      1103  AGCGCT               AGC^GCT
             1537  AGCGCT              
BanI           42  GGTGCC               G^G[CT][GA]CC
             1491  GGTGCC              
BclI          635  TGATCA               T^GATCA
             1118  TGATCA              
BfaI          389  CTAG                 C^TAG
             1393  CTAG                
BsiEI         920  CGGCCG               CG[GA][CT]^CG
             1460  CGGCCG              
BsmAI         399  GAGAC                GTCTC(1/5)
             1371  GAGAC               
BspHI         560  TCATGA               T^CATGA
             1367  TCATGA              
BsrBI        1363  CCGCTC               CCGCTC(-3/-3)
             1132  GAGCGG              
BstYI          35  AGATCT               [GA]^GATC[CT]
               73  AGATCC              
DsaI           47  CCGTGG               C^C[GA][CT]GG
              548  CCATGG              
EaeI          920  CGGCCG               [CT]^GGCC[GA]
             1460  CGGCCG              
EagI          920  CGGCCG               C^GGCCG
             1460  CGGCCG              
EclXI         920  CGGCCG               C^GGCCG
             1460  CGGCCG              
Eco47III     1103  AGCGCT               AGC^GCT
             1537  AGCGCT              
Eco52I        920  CGGCCG               C^GGCCG
             1460  CGGCCG              
FbaI          635  TGATCA               T^GATCA
             1118  TGATCA              
MaeI          389  CTAG                 C^TAG
             1393  CTAG                
MflI           35  AGATCT               [GA]^GATC[CT]
               73  AGATCC              
MseI          437  TTAA                 T^TAA
              503  TTAA                
PflMI        1089  CCAGGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1101  CCAGCGCTTGG         
RcaI          560  TCATGA               T^CATGA
             1367  TCATGA              
Tru9I         437  TTAA                 T^TAA
              503  TTAA                
Van91I       1089  CCAGGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1101  CCAGCGCTTGG         
XhoII          35  AGATCT               [GA]^GATC[CT]
               73  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   77 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       AspHI       AvaII       BbvI        
BcnI        BmyI        BpmI        BsaHI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       EarI        EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeII       MboI        MboII       MnlI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        PpuMI       PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       TaqI        TseI        
TspRI       TthHB8I     
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