Restriction map for pF1KE3228
 (Linear) (Six-base) MAPSORT of: KIEE3228.seq  Check: 5671  from: 1  to: 1560



KIEE3228.orf

 With 182 enzymes: SgfI * 

                        June 19, 2019 14:13  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     892     922    1560 
   Size:        892      30     638

  Fragments arranged by size: 

                 892     638      30
 
AfeI AGC'GCT

Cuts at:      0    1105    1539    1560 
   Size:       1105     434      21

  Fragments arranged by size: 

                1105     434      21
 
AleI CACnn'nnGTG

Cuts at:      0     199    1560 
   Size:        199    1361
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1294    1326    1560 
   Size:       1294      32     234

  Fragments arranged by size: 

                1294     234      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      77     870    1095    1560 
   Size:         77     793     225     465

  Fragments arranged by size: 

                 793     465     225      77
 
AvaI C'yCGr_G

Cuts at:      0     134    1560 
   Size:        134    1426
 
BanI G'GyrC_C

Cuts at:      0      42    1491    1560 
   Size:         42    1449      69

  Fragments arranged by size: 

                1449      69      42
 
BclI T'GATC_A

Cuts at:      0     635    1118    1560 
   Size:        635     483     442

  Fragments arranged by size: 

                 635     483     442
 
BglI GCCn_nnn'nGGC

Cuts at:      0     761    1560 
   Size:        761     799
 
BglII A'GATC_T

Cuts at:      0      35    1560 
   Size:         35    1525
 
Bme1580I G_kGCm'C

Cuts at:      0      47    1496    1560 
   Size:         47    1449      64

  Fragments arranged by size: 

                1449      64      47
 
BmgBI CAC'GTC

Cuts at:      0     651    1560 
   Size:        651     909
 
BmtI G_CTAG'C

Cuts at:      0    1396    1560 
   Size:       1396     164
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     495     766    1150    1533    1560 
   Size:        495     271     384     383      27

  Fragments arranged by size: 

                 495     384     383     271      27
 
Bpu10I CC'TnA_GC

Cuts at:      0     309    1560 
   Size:        309    1251
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     339    1560 
   Size:        339    1221
 
BsaAI yAC'GTr

Cuts at:      0     729    1560 
   Size:        729     831
 
BsaBI GATnn'nnATC

Cuts at:      0     721    1560 
   Size:        721     839
 
BsaHI Gr'CG_yC

Cuts at:      0     188     980    1261    1560 
   Size:        188     792     281     299

  Fragments arranged by size: 

                 792     299     281     188
 
BsaWI w'CCGG_w

Cuts at:      0     293    1560 
   Size:        293    1267
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     411     874    1003    1018    1042    1228    1453    1536 
   Size:        411     463     129      15      24     186     225      83

Cuts at:   1536    1560 
   Size:         24

  Fragments arranged by size: 

                 463     411     225     186     129      83      24      24
                  15
 
BseYI C'CCAG_C

Cuts at:      0    1471    1560 
   Size:       1471      89
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     700     751    1229    1302    1560 
   Size:         49     651      51     478      73     258

  Fragments arranged by size: 

                 651     478     258      73      51      49
 
BsiEI CG_ry'CG

Cuts at:      0     923    1463    1560 
   Size:        923     540      97

  Fragments arranged by size: 

                 923     540      97
 
BsiHKAI G_wGCw'C

Cuts at:      0     776     843     910    1560 
   Size:        776      67      67     650

  Fragments arranged by size: 

                 776     650      67      67
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1365    1560 
   Size:       1365     195
 
Bsp1286I G_dGCh'C

Cuts at:      0      47     776     843     910    1496    1560 
   Size:         47     729      67      67     586      64

  Fragments arranged by size: 

                 729     586      67      67      64      47
 
BspEI T'CCGG_A

Cuts at:      0     293    1560 
   Size:        293    1267
 
BspHI T'CATG_A

Cuts at:      0     560    1367    1560 
   Size:        560     807     193

  Fragments arranged by size: 

                 807     560     193
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     887    1560 
   Size:        887     673
 
BsrBI CCG'CTC

Cuts at:      0    1134    1365    1560 
   Size:       1134     231     195

  Fragments arranged by size: 

                1134     231     195
 
BsrDI GCAATG_nn'

Cuts at:      0     307    1560 
   Size:        307    1253
 
BsrFI r'CCGG_y

Cuts at:      0     755    1560 
   Size:        755     805
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     495    1560 
   Size:        495    1065
 
BstYI r'GATC_y

Cuts at:      0      35      73    1560 
   Size:         35      38    1487

  Fragments arranged by size: 

                1487      38      35
 
Bsu36I CC'TnA_GG

Cuts at:      0    1025    1560 
   Size:       1025     535
 
BtgI C'CryG_G

Cuts at:      0      47     548    1560 
   Size:         47     501    1012

  Fragments arranged by size: 

                1012     501      47
 
BtsI GCAGTG_nn'

Cuts at:      0     955    1560 
   Size:        955     605
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     979    1560 
   Size:        979     581
 
EaeI y'GGCC_r

Cuts at:      0     920    1460    1560 
   Size:        920     540     100

  Fragments arranged by size: 

                 920     540     100
 
EagI C'GGCC_G

Cuts at:      0     920    1460    1560 
   Size:        920     540     100

  Fragments arranged by size: 

                 920     540     100
 
EarI CTCTTCn'nnn_

Cuts at:      0     559     898    1244    1560 
   Size:        559     339     346     316

  Fragments arranged by size: 

                 559     346     339     316
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     253    1560 
   Size:        253    1307
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     495     766     892     922    1150    1533    1560 
   Size:        495     271     126      30     228     383      27

  Fragments arranged by size: 

                 495     383     271     228     126      30      27
 
EcoO109I rG'GnC_Cy

Cuts at:      0      59     876    1092    1354    1560 
   Size:         59     817     216     262     206

  Fragments arranged by size: 

                 817     262     216     206      59
 
HaeII r_GCGC'y

Cuts at:      0     931     961    1107    1541    1560 
   Size:        931      30     146     434      19

  Fragments arranged by size: 

                 931     434     146      30      19
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     392     424     520     552    1560 
   Size:        392      32      96      32    1008

  Fragments arranged by size: 

                1008     392      96      32      32
 
MslI CAynn'nnrTG

Cuts at:      0     115     199     727    1560 
   Size:        115      84     528     833

  Fragments arranged by size: 

                 833     528     115      84
 
MspA1I CmG'CkG

Cuts at:      0     900    1475    1503    1560 
   Size:        900     575      28      57

  Fragments arranged by size: 

                 900     575      57      28
 
NaeI GCC'GGC

Cuts at:      0     757    1560 
   Size:        757     803
 
NcoI C'CATG_G

Cuts at:      0     548    1560 
   Size:        548    1012
 
NgoMIV G'CCGG_C

Cuts at:      0     755    1560 
   Size:        755     805
 
NheI G'CTAG_C

Cuts at:      0    1392    1560 
   Size:       1392     168
 
NspI r_CATG'y

Cuts at:      0    1447    1560 
   Size:       1447     113
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1095    1107    1560 
   Size:       1095      12     453

  Fragments arranged by size: 

                1095     453      12
 
PfoI T'CCnGG_A

Cuts at:      0    1196    1528    1549    1560 
   Size:       1196     332      21      11

  Fragments arranged by size: 

                1196     332      21      11
 
PmlI CAC'GTG

Cuts at:      0     729    1560 
   Size:        729     831
 
PpuMI rG'GwC_Cy

Cuts at:      0     876    1092    1354    1560 
   Size:        876     216     262     206

  Fragments arranged by size: 

                 876     262     216     206
 
PshAI GACnn'nnGTC

Cuts at:      0    1264    1560 
   Size:       1264     296
 
PvuII CAG'CTG

Cuts at:      0     900    1475    1503    1560 
   Size:        900     575      28      57

  Fragments arranged by size: 

                 900     575      57      28
 
SapI GCTCTTCn'nnn_

Cuts at:      0     898    1560 
   Size:        898     662
 
ScaI AGT'ACT

Cuts at:      0    1057    1560 
   Size:       1057     503
 
SexAI A'CCwGG_T

Cuts at:      0    1094    1560 
   Size:       1094     466
 
SfcI C'TryA_G

Cuts at:      0     473    1560 
   Size:        473    1087
 
SmaI CCC'GGG

Cuts at:      0     136    1560 
   Size:        136    1424
 
SmlI C'TyrA_G

Cuts at:      0     318    1560 
   Size:        318    1242
 
StuI AGG'CCT

Cuts at:      0     809    1560 
   Size:        809     751
 
StyI C'CwwG_G

Cuts at:      0     548    1560 
   Size:        548    1012
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      30    1560 
   Size:         30    1530
 
TatI w'GTAC_w

Cuts at:      0     242     707    1055    1146    1560 
   Size:        242     465     348      91     414

  Fragments arranged by size: 

                 465     414     348     242      91
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     288     320    1560 
   Size:        288      32    1240

  Fragments arranged by size: 

                1240     288      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1096    1560 
   Size:       1096     464
 
XmaI C'CCGG_G

Cuts at:      0     134    1560 
   Size:        134    1426
 

 Enzymes that do cut:

     AcuI     AfeI     AleI     AloI    AlwNI     AvaI     BanI     BclI
     BglI    BglII Bme1580I    BmgBI     BmtI     BpmI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BstXI    BstYI   Bsu36I     BtgI     BtsI     DrdI     EaeI
     EagI     EarI     EciI  Eco57MI EcoO109I    HaeII    Hin4I     MslI
   MspA1I     NaeI     NcoI   NgoMIV     NheI     NspI    PflMI     PfoI
     PmlI    PpuMI    PshAI    PvuII     SapI     ScaI    SexAI     SfcI
     SmaI     SmlI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AlfI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI    BfrBI     BlpI     BmrI     BplI     BsaI    BsaXI    BsiWI
     BsmI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I
    BtgZI     ClaI    CspCI     DraI   DraIII  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     NarI     NdeI
     NotI     NruI     NsiI     PacI     PasI     PciI     PmeI     PpiI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SbfI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     XbaI     XcmI
     XhoI     XmnI     ZraI
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