Restriction map for pF1KE3768
##################################
# NO CUT ENZYMES (   83 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AfaI        AflII       AgeI        ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AviII       
AvrII       BciVI       BclI        BfrI        BglII       
BlnI        BsiWI       Bsp1407I    BspDI       BspHI       
BsrDI       BsrGI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     ClaI        CpoI        
DraI        DraIII      DrdI        EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HindIII     HpaI        KpnI        MfeI        MseI        
MunI        NdeI        NheI        NruI        NsiI        
NspV        PacI        PaeR7I      PinAI       PmeI        
PshAI       PshBI       Psp1406I    RcaI        RsaI        
RsrII       SacI        SalI        SapI        SbfI        
ScaI        SexAI       SfuI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        SwaI        Tru9I       
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AccIII       2754  TCCGGA               T^CCGGA
AhdI          664  GACCTCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        539  GTGCAC               G^TGCAC
AocI          477  CCTTAGG              CC^T[ACGT]AGG
ApaLI         539  GTGCAC               G^TGCAC
AspEI         664  GACCTCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI           23  TGGCCA               TGG^CCA
BamHI        1489  GGATCC               G^GATCC
BcgI          987  CGAGCCCTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI         1332  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1332  GCTGAGC              GC^T[ACGT]AGC
BsaBI        1290  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        2754  TCCGGA               [AT]^CCGG[AT]
BseAI        2754  TCCGGA               T^CCGGA
BsmBI         826  CGTCTC               CGTCTC(1/5)
BsmI         1012  GAATGC               GAATGC(1/-1)
BspEI        2754  TCCGGA               T^CCGGA
BspLU11I     1157  ACATGT               A^CATGT
Bsu36I        477  CCTTAGG              CC^T[ACGT]AGG
CelII        1332  GCTGAGC              GC^T[ACGT]AGC
Eam1105I      664  GACCTCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        477  CCTTAGG              CC^T[ACGT]AGG
HincII        589  GTCAAC               GT[CT]^[GA]AC
HindII        589  GTCAAC               GT[CT]^[GA]AC
MamI         1290  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI         2272  ACGCGT               A^CGCGT
MluNI          23  TGGCCA               TGG^CCA
MroI         2754  TCCGGA               T^CCGGA
MscI           23  TGGCCA               TGG^CCA
NcoI         2532  CCATGG               C^CATGG
NotI          194  GCGGCCGC             GC^GGCCGC
PvuI          120  CGATCG               CGAT^CG
SfiI         2304  GGCCGCACCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI        2473  CACCGGCG             C[GA]^CCGG[CT]G
SphI         2452  GCATGC               GCATG^C
StuI         1460  AGGCCT               AGG^CCT
TfiI         1149  GATTC                G^A[AT]TC
Tsp509I       471  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AflIII       1157  ACATGT               A^C[GA][CT]GT
             2272  ACGCGT              
AlwNI        1270  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2584  CAGGCCCTG           
Aor51HI       784  AGCGCT               AGC^GCT
             1235  AGCGCT              
AspHI         539  GTGCAC               G[AT]GC[AT]^C
              727  GTGCTC              
AspI         1893  GACCCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1962  GACGCCGTC           
BbrPI         580  CACGTG               CAC^GTG
              978  CACGTG              
BbvCI        2160  CCTCAGC              CCTCAGC(-5/-2)
             1536  GCTGAGG             
BsaI         2655  GAGACC               GGTCTC(1/5)
             2703  GGTCTC              
BsiHKAI       539  GTGCAC               G[AT]GC[AT]^C
              727  GTGCTC              
BsrBI        1979  CCGCTC               CCGCTC(-3/-3)
             1702  GAGCGG              
BssHII       2080  GCGCGC               G^CGCGC
             2082  GCGCGC              
BstYI         275  AGATCC               [GA]^GATC[CT]
             1489  GGATCC              
EarI          835  CTCTTC               CTCTTC(1/4)
             2125  CTCTTC              
Eco47III      784  AGCGCT               AGC^GCT
             1235  AGCGCT              
EcoNI        1002  CCTCCTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1692  CCTGCAGAAGG         
HphI         2095  GGTGA                GGTGA(8/7)
             2723  TCACC               
Ksp632I       835  CTCTTC               CTCTTC(1/4)
             2125  CTCTTC              
MflI          275  AGATCC               [GA]^GATC[CT]
             1489  GGATCC              
NspI         1157  ACATGT               [GA]CATG^[CT]
             2452  GCATGC              
PflFI        1893  GACCCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1962  GACGCCGTC           
PflMI          26  CCAAGGAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2485  CCATCGGATGG         
PleI         1554  GACTC                GAGTC(4/5)
             1111  GAGTC               
PmaCI         580  CACGTG               CAC^GTG
              978  CACGTG              
PmlI          580  CACGTG               CAC^GTG
              978  CACGTG              
Tth111I      1893  GACCCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1962  GACGCCGTC           
Van91I         26  CCAAGGAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2485  CCATCGGATGG         
XhoII         275  AGATCC               [GA]^GATC[CT]
             1489  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (  110 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AlwI        
ApaI        AvaI        AvaII       BanI        BanII       
BbeI        BbsI        BbvI        BcnI        BfaI        
BglI        BmyI        BpmI        BpuAI       BsaAI       
BsaHI       BsaJI       BseRI       BsgI        BsiEI       
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrFI       BsrI        BssKI       
BssSI       BstF5I      BstNI       BstUI       BstXI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EagI        
EclXI       Eco52I      EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       ItaI        KasI        KspI        
MaeI        MaeII       MaeIII      MboI        MboII       
MnlI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NaeI        NarI        NciI        
NdeII       NgoMIV      NlaIII      NlaIV       PpuMI       
PstI        PvuII       SacII       Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SfoI        SmaI        
SmlI        StyI        TaqI        TseI        Tsp45I      
TspRI       TthHB8I     XcmI        XmaCI       XmaI        
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