Restriction map for pF1KE3768
 (Linear) (Six-base) MAPSORT of: KIEE3768.seq  Check: 8923  from: 1  to: 2862



KIEE3768.orf

 With 182 enzymes: SgfI * 

                        April 23, 2019 13:01  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     411     594    1325    2862 
   Size:        411     183     731    1537

  Fragments arranged by size: 

                1537     731     411     183
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1747    2862 
   Size:       1747    1115
 
AfeI AGC'GCT

Cuts at:      0     786    1237    2862 
   Size:        786     451    1625

  Fragments arranged by size: 

                1625     786     451
 
AflIII A'CryG_T

Cuts at:      0    1157    2272    2862 
   Size:       1157    1115     590

  Fragments arranged by size: 

                1157    1115     590
 
AhdI GACnn_n'nnGTC

Cuts at:      0     669    2862 
   Size:        669    2193
 
AleI CACnn'nnGTG

Cuts at:      0    2236    2862 
   Size:       2236     626
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1405    1888    2862 
   Size:       1405     483     974

  Fragments arranged by size: 

                1405     974     483
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1275    2589    2862 
   Size:       1275    1314     273

  Fragments arranged by size: 

                1314    1275     273
 
ApaI G_GGCC'C

Cuts at:      0      56     345    1628    1682    2206    2771    2850    2862 
   Size:         56     289    1283      54     524     565      79      12

  Fragments arranged by size: 

                1283     565     524     289      79      56      54      12
            
 
ApaLI G'TGCA_C

Cuts at:      0     539    2862 
   Size:        539    2323
 
AvaI C'yCGr_G

Cuts at:      0     530     611     985    1349    1935    2518    2652    2862 
   Size:        530      81     374     364     586     583     134     210

  Fragments arranged by size: 

                 586     583     530     374     364     210     134      81
            
 
BamHI G'GATC_C

Cuts at:      0    1489    2862 
   Size:       1489    1373
 
BanI G'GyrC_C

Cuts at:      0     235     484     570     810    1947    1996    2062    2220 
   Size:        235     249      86     240    1137      49      66     158

Cuts at:   2220    2471    2862 
   Size:        251     391

  Fragments arranged by size: 

                1137     391     251     249     240     235     158      86
                  66      49
 
BanII G_rGCy'C

Cuts at:      0      56     345     453     992    1628    1682    1931    2206 
   Size:         56     289     108     539     636      54     249     275

Cuts at:   2206    2525    2764    2771    2850    2862 
   Size:        319     239       7      79      12

  Fragments arranged by size: 

                 636     539     319     289     275     249     239     108
                  79      56      54      12       7
 
BbeI G_GCGC'C

Cuts at:      0     239     488     814    1951    2000    2066    2224    2862 
   Size:        239     249     326    1137      49      66     158     638

  Fragments arranged by size: 

                1137     638     326     249     239     158      66      49
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     201    1139    2201    2862 
   Size:        201     938    1062     661

  Fragments arranged by size: 

                1062     938     661     201
 
BbvCI CC'TCA_GC

Cuts at:      0    1537    2161    2862 
   Size:       1537     624     701

  Fragments arranged by size: 

                1537     701     624
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     976    1010    2862 
   Size:        976      34    1852

  Fragments arranged by size: 

                1852     976      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     203     808    2071    2311    2862 
   Size:        203     605    1263     240     551

  Fragments arranged by size: 

                1263     605     551     240     203
 
BlpI GC'TnA_GC

Cuts at:      0    1333    2862 
   Size:       1333    1529
 
Bme1580I G_kGCm'C

Cuts at:      0      40      56     345     543     650     985    1628    1682 
   Size:         40      16     289     198     107     335     643      54

Cuts at:   1682    1770    1877    2206    2474    2771    2837    2850    2862 
   Size:         88     107     329     268     297      66      13      12

  Fragments arranged by size: 

                 643     335     329     297     289     268     198     107
                 107      88      66      54      40      16      13      12
            
 
BmgBI CAC'GTC

Cuts at:      0    2070    2199    2442    2862 
   Size:       2070     129     243     420

  Fragments arranged by size: 

                2070     420     243     129
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     603    2862 
   Size:        603    2259
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     244     712     879    1027    1129    1165    2410    2416 
   Size:        244     468     167     148     102      36    1245       6

Cuts at:   2416    2862 
   Size:        446

  Fragments arranged by size: 

                1245     468     446     244     167     148     102      36
                   6
 
Bpu10I CC'TnA_GC

Cuts at:      0    1537    2161    2862 
   Size:       1537     624     701

  Fragments arranged by size: 

                1537     701     624
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     652     715    1378    2862 
   Size:        652      63     663    1484

  Fragments arranged by size: 

                1484     663     652      63
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2649    2709    2862 
   Size:       2649      60     153

  Fragments arranged by size: 

                2649     153      60
 
BsaAI yAC'GTr

Cuts at:      0     225     582     980    2862 
   Size:        225     357     398    1882

  Fragments arranged by size: 

                1882     398     357     225
 
BsaBI GATnn'nnATC

Cuts at:      0    1294    2862 
   Size:       1294    1568
 
BsaHI Gr'CG_yC

Cuts at:      0     206     236     485     811     920    1948    1963    1997 
   Size:        206      30     249     326     109    1028      15      34

Cuts at:   1997    2063    2221    2482    2862 
   Size:         66     158     261     380

  Fragments arranged by size: 

                1028     380     326     261     249     206     158     109
                  66      34      30      15
 
BsaWI w'CCGG_w

Cuts at:      0    2754    2862 
   Size:       2754     108
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     409     439    1605    1635    2251    2281    2862 
   Size:        409      30    1166      30     616      30     581

  Fragments arranged by size: 

                1166     616     581     409      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      19      22      25     112     316     373    1219    1222 
   Size:         19       3       3      87     204      57     846       3

Cuts at:   1222    1261    1727    1732    1852    2113    2862 
   Size:         39     466       5     120     261     749

  Fragments arranged by size: 

                 846     749     466     261     204     120      87      57
                  39      19       5       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     190     214    1016    1519    1681    1734    2043    2365 
   Size:        190      24     802     503     162      53     309     322

Cuts at:   2365    2499    2595    2737    2773    2862 
   Size:        134      96     142      36      89

  Fragments arranged by size: 

                 802     503     322     309     190     162     142     134
                  96      89      53      36      24
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     230     622    1297    2800    2862 
   Size:        230     392     675    1503      62

  Fragments arranged by size: 

                1503     675     392     230      62
 
BsiEI CG_ry'CG

Cuts at:      0     123     198    2090    2145    2187    2315    2665    2715 
   Size:        123      75    1892      55      42     128     350      50

Cuts at:   2715    2862 
   Size:        147

  Fragments arranged by size: 

                1892     350     147     128     123      75      55      50
                  42
 
BsiHKAI G_wGCw'C

Cuts at:      0     543     731    2862 
   Size:        543     188    2131

  Fragments arranged by size: 

                2131     543     188
 
BsmI GAATG_Cn'

Cuts at:      0    1018    2862 
   Size:       1018    1844
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     832    2862 
   Size:        832    2030
 
Bsp1286I G_dGCh'C

Cuts at:      0      40      56     345     453     543     650     731     985 
   Size:         40      16     289     108      90     107      81     254

Cuts at:    985     992    1628    1682    1770    1877    1931    2206    2474 
   Size:          7     636      54      88     107      54     275     268

Cuts at:   2474    2525    2764    2771    2837    2850    2862 
   Size:         51     239       7      66      13      12

  Fragments arranged by size: 

                 636     289     275     268     254     239     108     107
                 107      90      88      81      66      54      54      51
                  40      16      13      12       7       7
 
BspEI T'CCGG_A

Cuts at:      0    2754    2862 
   Size:       2754     108
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     411     550     594    1325    2862 
   Size:        411     139      44     731    1537

  Fragments arranged by size: 

                1537     731     411     139      44
 
BsrBI CCG'CTC

Cuts at:      0    1704    1981    2862 
   Size:       1704     277     881

  Fragments arranged by size: 

                1704     881     277
 
BsrFI r'CCGG_y

Cuts at:      0    2140    2310    2474    2606    2664    2862 
   Size:       2140     170     164     132      58     198

  Fragments arranged by size: 

                2140     198     170     164     132      58
 
BssHII G'CGCG_C

Cuts at:      0    2080    2082    2862 
   Size:       2080       2     780

  Fragments arranged by size: 

                2080     780       2
 
BssSI C'ACGA_G

Cuts at:      0     286    2236    2546    2862 
   Size:        286    1950     310     316

  Fragments arranged by size: 

                1950     316     310     286
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2043    2379    2539    2793    2862 
   Size:       2043     336     160     254      69

  Fragments arranged by size: 

                2043     336     254     160      69
 
BstYI r'GATC_y

Cuts at:      0     275    1489    2862 
   Size:        275    1214    1373

  Fragments arranged by size: 

                1373    1214     275
 
Bsu36I CC'TnA_GG

Cuts at:      0     478    2862 
   Size:        478    2384
 
BtgI C'CryG_G

Cuts at:      0      47      65     251     929    1032    1559    1617    1951 
   Size:         47      18     186     678     103     527      58     334

Cuts at:   1951    2152    2181    2466    2525    2532    2850    2862 
   Size:        201      29     285      59       7     318      12

  Fragments arranged by size: 

                 678     527     334     318     285     201     186     103
                  59      58      47      29      18      12       7
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1788    2862 
   Size:       1788    1074
 
BtsI GCAGTG_nn'

Cuts at:      0     413     855    1587    1599    1915    2278    2788    2862 
   Size:        413     442     732      12     316     363     510      74

  Fragments arranged by size: 

                 732     510     442     413     363     316      74      12
            
 
EaeI y'GGCC_r

Cuts at:      0      23     195     926    1039    2072    2087    2142    2184 
   Size:         23     172     731     113    1033      15      55      42

Cuts at:   2184    2228    2312    2662    2712    2862 
   Size:         44      84     350      50     150

  Fragments arranged by size: 

                1033     731     350     172     150     113      84      55
                  50      44      42      23      15
 
EagI C'GGCC_G

Cuts at:      0     195    2087    2142    2184    2312    2662    2712    2862 
   Size:        195    1892      55      42     128     350      50     150

  Fragments arranged by size: 

                1892     350     195     150     128      55      50      42
            
 
EarI CTCTTCn'nnn_

Cuts at:      0     841    2131    2862 
   Size:        841    1290     731

  Fragments arranged by size: 

                1290     841     731
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2493    2862 
   Size:       2493     369
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     244     712     879    1027    1129    1165    1747    2410 
   Size:        244     468     167     148     102      36     582     663

Cuts at:   2410    2416    2862 
   Size:          6     446

  Fragments arranged by size: 

                 663     582     468     446     244     167     148     102
                  36       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1006    1696    2862 
   Size:       1006     690    1166

  Fragments arranged by size: 

                1166    1006     690
 
EcoO109I rG'GnC_Cy

Cuts at:      0      52     342     399     663     794     900    1020    1625 
   Size:         52     290      57     264     131     106     120     605

Cuts at:   1625    1678    2157    2203    2412    2586    2768    2846    2862 
   Size:         53     479      46     209     174     182      78      16

  Fragments arranged by size: 

                 605     479     290     264     209     182     174     131
                 120     106      78      57      53      52      46      16
            
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1389    2862 
   Size:       1389    1473
 
HaeII r_GCGC'y

Cuts at:      0     239     488     788     814    1239    1951    2000    2010 
   Size:        239     249     300      26     425     712      49      10

Cuts at:   2010    2023    2066    2112    2224    2500    2862 
   Size:         13      43      46     112     276     362

  Fragments arranged by size: 

                 712     425     362     300     276     249     239     112
                  49      46      43      26      13      10
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     105     137     655     687    2251    2283    2862 
   Size:        105      32     518      32    1564      32     579

  Fragments arranged by size: 

                1564     579     518     105      32      32      32
 
HincII GTy'rAC

Cuts at:      0     591    2862 
   Size:        591    2271
 
KasI G'GCGC_C

Cuts at:      0     235     484     810    1947    1996    2062    2220    2862 
   Size:        235     249     326    1137      49      66     158     642

  Fragments arranged by size: 

                1137     642     326     249     235     158      66      49
            
 
MluI A'CGCG_T

Cuts at:      0    2272    2862 
   Size:       2272     590
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1492    2282    2862 
   Size:       1492     790     580

  Fragments arranged by size: 

                1492     790     580
 
MscI TGG'CCA

Cuts at:      0      25    2862 
   Size:         25    2837
 
MslI CAynn'nnrTG

Cuts at:      0     313     635     778    2236    2803    2862 
   Size:        313     322     143    1458     567      59

  Fragments arranged by size: 

                1458     567     322     313     143      59
 
MspA1I CmG'CkG

Cuts at:      0      49      67     194     244     253     264     415    1254 
   Size:         49      18     127      50       9      11     151     839

Cuts at:   1254    1332    1619    2106    2154    2468    2508    2527    2862 
   Size:         78     287     487      48     314      40      19     335

  Fragments arranged by size: 

                 839     487     335     314     287     151     127      78
                  50      49      48      40      19      18      11       9
            
 
NaeI GCC'GGC

Cuts at:      0    2142    2608    2666    2862 
   Size:       2142     466      58     196

  Fragments arranged by size: 

                2142     466     196      58
 
NarI GG'CG_CC

Cuts at:      0     236     485     811    1948    1997    2063    2221    2862 
   Size:        236     249     326    1137      49      66     158     641

  Fragments arranged by size: 

                1137     641     326     249     236     158      66      49
            
 
NcoI C'CATG_G

Cuts at:      0    2532    2862 
   Size:       2532     330
 
NgoMIV G'CCGG_C

Cuts at:      0    2140    2606    2664    2862 
   Size:       2140     466      58     198

  Fragments arranged by size: 

                2140     466     198      58
 
NotI GC'GGCC_GC

Cuts at:      0     195    2862 
   Size:        195    2667
 
NspI r_CATG'y

Cuts at:      0    1161    2456    2862 
   Size:       1161    1295     406

  Fragments arranged by size: 

                1295    1161     406
 
PasI CC'CwG_GG

Cuts at:      0    2764    2862 
   Size:       2764      98
 
PciI A'CATG_T

Cuts at:      0    1157    2862 
   Size:       1157    1705
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      32    2491    2862 
   Size:         32    2459     371

  Fragments arranged by size: 

                2459     371      32
 
PfoI T'CCnGG_A

Cuts at:      0     689    1004    1241    2862 
   Size:        689     315     237    1621

  Fragments arranged by size: 

                1621     689     315     237
 
PmlI CAC'GTG

Cuts at:      0     582     980    2862 
   Size:        582     398    1882

  Fragments arranged by size: 

                1882     582     398
 
PpuMI rG'GwC_Cy

Cuts at:      0     399     663     794     900    1020    2412    2862 
   Size:        399     264     131     106     120    1392     450

  Fragments arranged by size: 

                1392     450     399     264     131     120     106
 
PspOMI G'GGCC_C

Cuts at:      0      52     341    1624    1678    2202    2767    2846    2862 
   Size:         52     289    1283      54     524     565      79      16

  Fragments arranged by size: 

                1283     565     524     289      79      54      52      16
            
 
PstI C_TGCA'G

Cuts at:      0     296     458    1130    1697    2862 
   Size:        296     162     672     567    1165

  Fragments arranged by size: 

                1165     672     567     296     162
 
PvuI CG_AT'CG

Cuts at:      0     123    2862 
   Size:        123    2739
 
PvuII CAG'CTG

Cuts at:      0     264    1254    1332    2862 
   Size:        264     990      78    1530

  Fragments arranged by size: 

                1530     990     264      78
 
SacII CC_GC'GG

Cuts at:      0      50      68     254    1620    2155    2469    2528    2862 
   Size:         50      18     186    1366     535     314      59     334

  Fragments arranged by size: 

                1366     535     334     314     186      59      50      18
            
 
SanDI GG'GwC_CC

Cuts at:      0    1020    2862 
   Size:       1020    1842
 
SfcI C'TryA_G

Cuts at:      0     292     454    1126    1693    2862 
   Size:        292     162     672     567    1169

  Fragments arranged by size: 

                1169     672     567     292     162
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    2311    2862 
   Size:       2311     551
 
SfoI GGC'GCC

Cuts at:      0     237     486     812    1949    1998    2064    2222    2862 
   Size:        237     249     326    1137      49      66     158     640

  Fragments arranged by size: 

                1137     640     326     249     237     158      66      49
            
 
SgrAI Cr'CCGG_yG

Cuts at:      0    2474    2862 
   Size:       2474     388
 
SmaI CCC'GGG

Cuts at:      0     532     613    1351    1937    2862 
   Size:        532      81     738     586     925

  Fragments arranged by size: 

                 925     738     586     532      81
 
SmlI C'TyrA_G

Cuts at:      0     667     730    1393    2862 
   Size:        667      63     663    1469

  Fragments arranged by size: 

                1469     667     663      63
 
SphI G_CATG'C

Cuts at:      0    2456    2862 
   Size:       2456     406
 
StuI AGG'CCT

Cuts at:      0    1462    2862 
   Size:       1462    1400
 
StyI C'CwwG_G

Cuts at:      0      26     923    1178    2532    2862 
   Size:         26     897     255    1354     330

  Fragments arranged by size: 

                1354     897     330     255      26
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1815    2367    2452    2862 
   Size:       1815     552      85     410

  Fragments arranged by size: 

                1815     552     410      85
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     409     441    1605    1637    2862 
   Size:        409      32    1164      32    1225

  Fragments arranged by size: 

                1225    1164     409      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1896    1965    2862 
   Size:       1896      69     897

  Fragments arranged by size: 

                1896     897      69
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     198     222    2379    2862 
   Size:        198      24    2157     483

  Fragments arranged by size: 

                2157     483     198      24
 
XmaI C'CCGG_G

Cuts at:      0     530     611    1349    1935    2862 
   Size:        530      81     738     586     927

  Fragments arranged by size: 

                 927     738     586     530      81
 

 Enzymes that do cut:

     AarI     AcuI     AfeI   AflIII     AhdI     AleI     AlfI    AlwNI
     ApaI    ApaLI     AvaI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI     BglI     BlpI Bme1580I    BmgBI     BmrI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspMI    BsrBI    BsrFI   BssHII    BssSI    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI     EaeI     EagI     EarI     EciI
  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I   HincII     KasI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NotI     NspI     PasI     PciI    PflMI     PfoI     PmlI
    PpuMI   PspOMI     PstI     PvuI    PvuII    SacII    SanDI     SfcI
     SfiI     SfoI    SgrAI     SmaI     SmlI     SphI     StuI     StyI
    TaqII     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII     AgeI     AloI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BciVI     BclI    BfrBI
    BglII     BmtI     BplI    BsiWI    BspHI    BsrDI    BsrGI   BstAPI
    BstBI   BstEII  BstZ17I     ClaI    CspCI     DraI   DraIII     DrdI
  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI  HindIII     HpaI
     KpnI     MfeI     NdeI     NheI     NruI     NsiI     PacI     PmeI
     PpiI    PshAI     PsiI    PspXI     PsrI    RsrII     SacI     SalI
     SapI     SbfI     ScaI    SexAI     SgfI    SnaBI     SpeI     SrfI
     SspI     SwaI     TatI     XbaI     XhoI     XmnI     ZraI
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