Restriction map for pF1KE3827
 (Linear) (Six-base) MAPSORT of: KIEE3827.seq  Check: 4960  from: 1  to: 8451



KIEE3827.orf

 With 182 enzymes: SgfI * 

                        July 16, 2019 14:21  ..

AccI GT'mk_AC

Cuts at:      0    6575    8451 
   Size:       6575    1876
 
AclI AA'CG_TT

Cuts at:      0    6285    8451 
   Size:       6285    2166
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      83      98     275     448    1019    1186    1321    1477 
   Size:         83      15     177     173     571     167     135     156

Cuts at:   1477    2551    2747    2813    2917    3644    4079    4115    4150 
   Size:       1074     196      66     104     727     435      36      35

Cuts at:   4150    4646    5545    6751    7288    8451 
   Size:        496     899    1206     537    1163

  Fragments arranged by size: 

                1206    1163    1074     899     727     571     537     496
                 435     196     177     173     167     156     135     104
                  83      66      36      35      15
 
AfeI AGC'GCT

Cuts at:      0    7611    8451 
   Size:       7611     840
 
AflII C'TTAA_G

Cuts at:      0    2971    8451 
   Size:       2971    5480
 
AflIII A'CryG_T

Cuts at:      0    3830    5765    8451 
   Size:       3830    1935    2686

  Fragments arranged by size: 

                3830    2686    1935
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1052    1308    6840    8451 
   Size:       1052     256    5532    1611

  Fragments arranged by size: 

                5532    1611    1052     256
 
AleI CACnn'nnGTG

Cuts at:      0    4627    8451 
   Size:       4627    3824
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     233    3587    4793    8451 
   Size:        233    3354    1206    3658

  Fragments arranged by size: 

                3658    3354    1206     233
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     135     167    2427    2459    2847    2879    3202    3234 
   Size:        135      32    2260      32     388      32     323      32

Cuts at:   3234    8451 
   Size:       5217

  Fragments arranged by size: 

                5217    2260     388     323     135      32      32      32
                  32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1432    1774    1993    2983    3048    7662    7761    7908 
   Size:       1432     342     219     990      65    4614      99     147

Cuts at:   7908    8451 
   Size:        543

  Fragments arranged by size: 

                4614    1432     990     543     342     219     147      99
                  65
 
ApaI G_GGCC'C

Cuts at:      0    3624    8451 
   Size:       3624    4827
 
ApaLI G'TGCA_C

Cuts at:      0     959    1561    8451 
   Size:        959     602    6890

  Fragments arranged by size: 

                6890     959     602
 
ApoI r'AATT_y

Cuts at:      0     387    1752    1911    2490    5782    6425    7037    8451 
   Size:        387    1365     159     579    3292     643     612    1414

  Fragments arranged by size: 

                3292    1414    1365     643     612     579     387     159
            
 
AseI AT'TA_AT

Cuts at:      0    5083    8451 
   Size:       5083    3368
 
AvaI C'yCGr_G

Cuts at:      0    7085    7704    7795    8451 
   Size:       7085     619      91     656

  Fragments arranged by size: 

                7085     656     619      91
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    3489    3522    8451 
   Size:       3489      33    4929

  Fragments arranged by size: 

                4929    3489      33
 
BamHI G'GATC_C

Cuts at:      0     915    4408    8099    8451 
   Size:        915    3493    3691     352

  Fragments arranged by size: 

                3691    3493     915     352
 
BanI G'GyrC_C

Cuts at:      0     422    2319    3051    4754    8451 
   Size:        422    1897     732    1703    3697

  Fragments arranged by size: 

                3697    1897    1703     732     422
 
BanII G_rGCy'C

Cuts at:      0     576     971    1036    1457    3308    3584    3602    3624 
   Size:        576     395      65     421    1851     276      18      22

Cuts at:   3624    3654    4234    4660    4901    7086    7604    7711    7931 
   Size:         30     580     426     241    2185     518     107     220

Cuts at:   7931    8132    8451 
   Size:        201     319

  Fragments arranged by size: 

                2185    1851     580     576     518     426     421     395
                 319     276     241     220     201     107      65      30
                  22      18
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     266    1643    2135    2528    5377    6301    8451 
   Size:        266    1377     492     393    2849     924    2150

  Fragments arranged by size: 

                2849    2150    1377     924     492     393     266
 
BbvCI CC'TCA_GC

Cuts at:      0     529     980    3521    4553    7606    8451 
   Size:        529     451    2541    1032    3053     845

  Fragments arranged by size: 

                3053    2541    1032     845     529     451
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     649     683    2017    2051    8451 
   Size:        649      34    1334      34    6400

  Fragments arranged by size: 

                6400    1334     649      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     592    2163    6163    8451 
   Size:        592    1571    4000    2288

  Fragments arranged by size: 

                4000    2288    1571     592
 
BclI T'GATC_A

Cuts at:      0    1185    4995    6146    6911    8451 
   Size:       1185    3810    1151     765    1540

  Fragments arranged by size: 

                3810    1540    1185    1151     765
 
BfrBI ATG'CAT

Cuts at:      0     504    8451 
   Size:        504    7947
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1937    2704    3100    7867    8451 
   Size:       1937     767     396    4767     584

  Fragments arranged by size: 

                4767    1937     767     584     396
 
BglII A'GATC_T

Cuts at:      0    5014    6528    7485    8441    8451 
   Size:       5014    1514     957     956      10

  Fragments arranged by size: 

                5014    1514     957     956      10
 
BlpI GC'TnA_GC

Cuts at:      0    3302    7669    8035    8451 
   Size:       3302    4367     366     416

  Fragments arranged by size: 

                4367    3302     416     366
 
Bme1580I G_kGCm'C

Cuts at:      0     930     963    1234    1565    1611    3624    4759    5387 
   Size:        930      33     271     331      46    2013    1135     628

Cuts at:   5387    7948    8451 
   Size:       2561     503

  Fragments arranged by size: 

                2561    2013    1135     930     628     503     331     271
                  46      33
 
BmgBI CAC'GTC

Cuts at:      0     539    1560    1566    4050    4596    8451 
   Size:        539    1021       6    2484     546    3855

  Fragments arranged by size: 

                3855    2484    1021     546     539       6
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1365    1402    4273    4334    5902    6466    7782    8111 
   Size:       1365      37    2871      61    1568     564    1316     329

Cuts at:   8111    8451 
   Size:        340

  Fragments arranged by size: 

                2871    1568    1365    1316     564     340     329      61
                  37
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    4296    7218    8151    8451 
   Size:       4296    2922     933     300

  Fragments arranged by size: 

                4296    2922     933     300
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     692     708     824    1403    1568    1724    1988    2615 
   Size:        692      16     116     579     165     156     264     627

Cuts at:   2615    3505    3818    4984    5765    6202    7762    7915    7984 
   Size:        890     313    1166     781     437    1560     153      69

Cuts at:   7984    8451 
   Size:        467

  Fragments arranged by size: 

                1560    1166     890     781     692     627     579     467
                 437     313     264     165     156     153     116      69
                  16
 
Bpu10I CC'TnA_GC

Cuts at:      0     529     980    1315    2768    3521    4025    4553    7606 
   Size:        529     451     335    1453     753     504     528    3053

Cuts at:   7606    8451 
   Size:        845

  Fragments arranged by size: 

                3053    1453     845     753     529     528     504     451
                 335
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     856    2287    3011    6034    6564    8014    8451 
   Size:        856    1431     724    3023     530    1450     437

  Fragments arranged by size: 

                3023    1450    1431     856     724     530     437
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     160    3705    7402    8451 
   Size:        160    3545    3697    1049

  Fragments arranged by size: 

                3697    3545    1049     160
 
BsaAI yAC'GTr

Cuts at:      0    6712    8451 
   Size:       6712    1739
 
BsaBI GATnn'nnATC

Cuts at:      0     782    6151    6988    8098    8451 
   Size:        782    5369     837    1110     353

  Fragments arranged by size: 

                5369    1110     837     782     353
 
BsaHI Gr'CG_yC

Cuts at:      0     604    8451 
   Size:        604    7847
 
BsaWI w'CCGG_w

Cuts at:      0     963    4405    4682    4739    8451 
   Size:        963    3442     277      57    3712

  Fragments arranged by size: 

                3712    3442     963     277      57
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     159     189    1316    1346    2427    2457    2847    2877 
   Size:        159      30    1127      30    1081      30     390      30

Cuts at:   2877    3100    3130    4829    4859    8451 
   Size:        223      30    1699      30    3592

  Fragments arranged by size: 

                3592    1699    1127    1081     390     223     159      30
                  30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     676    1096    1099    1738    3179    3760    4003    4615 
   Size:        676     420       3     639    1441     581     243     612

Cuts at:   4615    4618    4621    4850    6907    7594    7813    7894    7897 
   Size:          3       3     229    2057     687     219      81       3

Cuts at:   7897    7939    8140    8451 
   Size:         42     201     311

  Fragments arranged by size: 

                2057    1441     687     676     639     612     581     420
                 311     243     229     219     201      81      42       3
                   3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     519    2092    3624    4694    5696    7871    8451 
   Size:        519    1573    1532    1070    1002    2175     580

  Fragments arranged by size: 

                2175    1573    1532    1070    1002     580     519
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     229     628    2006    4789    7197    7909    8129    8451 
   Size:        229     399    1378    2783    2408     712     220     322

  Fragments arranged by size: 

                2783    2408    1378     712     399     322     229     220
            
 
BsiHKAI G_wGCw'C

Cuts at:      0     576     963    1015    1565    3110    3584    3602    3679 
   Size:        576     387      52     550    1545     474      18      77

Cuts at:   3679    3804    4660    4901    7604    7711    7931    8451 
   Size:        125     856     241    2703     107     220     520

  Fragments arranged by size: 

                2703    1545     856     576     550     520     474     387
                 241     220     125     107      77      52      18
 
BsmI GAATG_Cn'

Cuts at:      0     415     672    1298    3384    6282    6741    7446    8451 
   Size:        415     257     626    2086    2898     459     705    1005

  Fragments arranged by size: 

                2898    2086    1005     705     626     459     415     257
            
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     800    1964    6616    8451 
   Size:        800    1164    4652    1835

  Fragments arranged by size: 

                4652    1835    1164     800
 
Bsp1286I G_dGCh'C

Cuts at:      0     576     930     963     971    1015    1036    1234    1457 
   Size:        576     354      33       8      44      21     198     223

Cuts at:   1457    1565    1611    3110    3308    3584    3602    3624    3654 
   Size:        108      46    1499     198     276      18      22      30

Cuts at:   3654    3679    3804    4234    4660    4759    4901    5387    7086 
   Size:         25     125     430     426      99     142     486    1699

Cuts at:   7086    7604    7711    7931    7948    8132    8451 
   Size:        518     107     220      17     184     319

  Fragments arranged by size: 

                1699    1499     576     518     486     430     426     354
                 319     276     223     220     198     198     184     142
                 125     108     107      99      46      44      33      30
                  25      22      21      18      17       8
 
BspEI T'CCGG_A

Cuts at:      0    4682    8451 
   Size:       4682    3769
 
BspHI T'CATG_A

Cuts at:      0     183     786    1457    2889    6056    6650    8451 
   Size:        183     603     671    1432    3167     594    1801

  Fragments arranged by size: 

                3167    1801    1432     671     603     594     183
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1650    7560    7778    8451 
   Size:       1650    5910     218     673

  Fragments arranged by size: 

                5910    1650     673     218
 
BsrBI CCG'CTC

Cuts at:      0    3121    6186    7235    7854    7920    8031    8390    8451 
   Size:       3121    3065    1049     619      66     111     359      61

  Fragments arranged by size: 

                3121    3065    1049     619     359     111      66      61
            
 
BsrDI GCAATG_nn'

Cuts at:      0     170    1847    5760    8451 
   Size:        170    1677    3913    2691

  Fragments arranged by size: 

                3913    2691    1677     170
 
BsrGI T'GTAC_A

Cuts at:      0     130    4180    5041    6068    8451 
   Size:        130    4050     861    1027    2383

  Fragments arranged by size: 

                4050    2383    1027     861     130
 
BssSI C'ACGA_G

Cuts at:      0    7832    8451 
   Size:       7832     619
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     316    1019    1993    2962    8451 
   Size:        316     703     974     969    5489

  Fragments arranged by size: 

                5489     974     969     703     316
 
BstBI TT'CG_AA

Cuts at:      0    2494    8451 
   Size:       2494    5957
 
BstEII G'GTnAC_C

Cuts at:      0    2793    8451 
   Size:       2793    5658
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     686    1626    3594    3616    5922    8451 
   Size:        686     940    1968      22    2306    2529

  Fragments arranged by size: 

                2529    2306    1968     940     686      22
 
BstYI r'GATC_y

Cuts at:      0     915    2103    4408    4959    5014    6090    6528    6930 
   Size:        915    1188    2305     551      55    1076     438     402

Cuts at:   6930    7298    7485    8099    8441    8451 
   Size:        368     187     614     342      10

  Fragments arranged by size: 

                2305    1188    1076     915     614     551     438     402
                 368     342     187      55      10
 
Bsu36I CC'TnA_GG

Cuts at:      0    3725    3944    4651    8123    8451 
   Size:       3725     219     707    3472     328

  Fragments arranged by size: 

                3725    3472     707     328     219
 
BtgI C'CryG_G

Cuts at:      0     437     567     971    3509    3953    4539    5588    8451 
   Size:        437     130     404    2538     444     586    1049    2863

  Fragments arranged by size: 

                2863    2538    1049     586     444     437     404     130
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    4736    8451 
   Size:       4736    3715
 
BtsI GCAGTG_nn'

Cuts at:      0    1326    1430    2382    2552    3016    3802    4470    5487 
   Size:       1326     104     952     170     464     786     668    1017

Cuts at:   5487    5581    6502    8451 
   Size:         94     921    1949

  Fragments arranged by size: 

                1949    1326    1017     952     921     786     668     464
                 170     104      94
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    6294    6329    8451 
   Size:       6294      35    2122

  Fragments arranged by size: 

                6294    2122      35
 
DraI TTT'AAA

Cuts at:      0     831    4490    6290    6860    8451 
   Size:        831    3659    1800     570    1591

  Fragments arranged by size: 

                3659    1800    1591     831     570
 
DraIII CAC_nnn'GTG

Cuts at:      0     443    1019    2953    4342    8451 
   Size:        443     576    1934    1389    4109

  Fragments arranged by size: 

                4109    1934    1389     576     443
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1564    8451 
   Size:       1564    6887
 
EaeI y'GGCC_r

Cuts at:      0     564     942    2512    3071    7479    7763    8451 
   Size:        564     378    1570     559    4408     284     688

  Fragments arranged by size: 

                4408    1570     688     564     559     378     284
 
EarI CTCTTCn'nnn_

Cuts at:      0      59     995    1136    2679    2832    2840    3306    4217 
   Size:         59     936     141    1543     153       8     466     911

Cuts at:   4217    4376    4889    4949    5171    5224    5287    6655    7136 
   Size:        159     513      60     222      53      63    1368     481

Cuts at:   7136    7234    7241    7460    8432    8451 
   Size:         98       7     219     972      19

  Fragments arranged by size: 

                1543    1368     972     936     911     513     481     466
                 222     219     159     153     141      98      63      60
                  59      53      19       8       7
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    6103    8451 
   Size:       6103    2348
 
EcoICRI GAG'CTC

Cuts at:      0     574    3582    3600    4658    4899    7602    7709    7929 
   Size:        574    3008      18    1058     241    2703     107     220

Cuts at:   7929    8451 
   Size:        522

  Fragments arranged by size: 

                3008    2703    1058     574     522     241     220     107
                  18
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      83      98     275     448     692     708     824    1019 
   Size:         83      15     177     173     244      16     116     195

Cuts at:   1019    1186    1321    1403    1477    1568    1724    1988    2551 
   Size:        167     135      82      74      91     156     264     563

Cuts at:   2551    2615    2747    2813    2917    3505    3644    3818    4079 
   Size:         64     132      66     104     588     139     174     261

Cuts at:   4079    4115    4150    4646    4984    5545    5765    6202    6751 
   Size:         36      35     496     338     561     220     437     549

Cuts at:   6751    7288    7762    7915    7984    8451 
   Size:        537     474     153      69     467

  Fragments arranged by size: 

                 588     563     561     549     537     496     474     467
                 437     338     264     261     244     220     195     177
                 174     173     167     156     153     139     135     132
                 116     104      91      83      82      74      69      66
                  64      36      35      16      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    4683    6553    7519    7912    8065    8451 
   Size:       4683    1870     966     393     153     386

  Fragments arranged by size: 

                4683    1870     966     393     386     153
 
EcoO109I rG'GnC_Cy

Cuts at:      0     231     367    2620    3620    3621    3856    4021    4699 
   Size:        231     136    2253    1000       1     235     165     678

Cuts at:   4699    5413    7264    7758    7860    7905    8058    8236    8298 
   Size:        714    1851     494     102      45     153     178      62

Cuts at:   8298    8451 
   Size:        153

  Fragments arranged by size: 

                2253    1851    1000     714     678     494     235     231
                 178     165     153     153     136     102      62      45
                   1
 
EcoRI G'AATT_C

Cuts at:      0     387    1752    6425    7037    8451 
   Size:        387    1365    4673     612    1414

  Fragments arranged by size: 

                4673    1414    1365     612     387
 
EcoRV GAT'ATC

Cuts at:      0    4401    8451 
   Size:       4401    4050
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     250    2755    4312    5575    6787    6837    7120    8451 
   Size:        250    2505    1557    1263    1212      50     283    1331

  Fragments arranged by size: 

                2505    1557    1331    1263    1212     283     250      50
            
 
HaeII r_GCGC'y

Cuts at:      0    7613    7684    7755    8451 
   Size:       7613      71      71     696

  Fragments arranged by size: 

                7613     696      71      71
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     159     191     723     755     870     902    1038    1070 
   Size:        159      32     532      32     115      32     136      32

Cuts at:   1070    1294    1326    1334    1366    1828    1860    3307    3339 
   Size:        224      32       8      32     462      32    1447      32

Cuts at:   3339    3444    3476    4192    4224    4829    4861    4951    4980 
   Size:        105      32     716      32     605      32      90      29

Cuts at:   4980    4983    5012    5563    5595    5752    5784    5896    5928 
   Size:          3      29     551      32     157      32     112      32

Cuts at:   5928    6075    6107    6628    6660    6826    6858    6915    6947 
   Size:        147      32     521      32     166      32      57      32

Cuts at:   6947    7308    7340    8451 
   Size:        361      32    1111

  Fragments arranged by size: 

                1447    1111     716     605     551     532     521     462
                 361     224     166     159     157     147     136     115
                 112     105      90      57      32      32      32      32
                  32      32      32      32      32      32      32      32
                  32      32      32      32      32      32      32      29
                  29       8       3
 
HincII GTy'rAC

Cuts at:      0     536    2002    4932    5379    5837    6845    8339    8451 
   Size:        536    1466    2930     447     458    1008    1494     112

  Fragments arranged by size: 

                2930    1494    1466    1008     536     458     447     112
            
 
HindIII A'AGCT_T

Cuts at:      0    6703    8451 
   Size:       6703    1748
 
HpaI GTT'AAC

Cuts at:      0    8339    8451 
   Size:       8339     112
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     728     870    3180    3420    4239    4827    5923    7052 
   Size:        728     142    2310     240     819     588    1096    1129

Cuts at:   7052    7118    7262    8451 
   Size:         66     144    1189

  Fragments arranged by size: 

                2310    1189    1129    1096     819     728     588     240
                 144     142      66
 
MscI TGG'CCA

Cuts at:      0     944    3073    7481    7765    8451 
   Size:        944    2129    4408     284     686

  Fragments arranged by size: 

                4408    2129     944     686     284
 
MslI CAynn'nnrTG

Cuts at:      0    3592    3614    4463    4627    6655    6922    8451 
   Size:       3592      22     849     164    2028     267    1529

  Fragments arranged by size: 

                3592    2028    1529     849     267     164      22
 
MspA1I CmG'CkG

Cuts at:      0     569    1196    1546    1771    2908    3503    3628    3796 
   Size:        569     627     350     225    1137     595     125     168

Cuts at:   3796    4694    5423    5922    6456    6980    8013    8043    8451 
   Size:        898     729     499     534     524    1033      30     408

  Fragments arranged by size: 

                1137    1033     898     729     627     595     569     534
                 524     499     408     350     225     168     125      30
            
 
NcoI C'CATG_G

Cuts at:      0    3509    4539    8451 
   Size:       3509    1030    3912

  Fragments arranged by size: 

                3912    3509    1030
 
NdeI CA'TA_TG

Cuts at:      0    6300    8451 
   Size:       6300    2151
 
NsiI A_TGCA'T

Cuts at:      0     506    8451 
   Size:        506    7945
 
NspI r_CATG'y

Cuts at:      0    2000    3834    5769    8451 
   Size:       2000    1834    1935    2682

  Fragments arranged by size: 

                2682    2000    1935    1834
 
PasI CC'CwG_GG

Cuts at:      0    1027    3222    8451 
   Size:       1027    2195    5229

  Fragments arranged by size: 

                5229    2195    1027
 
PciI A'CATG_T

Cuts at:      0    3830    5765    8451 
   Size:       3830    1935    2686

  Fragments arranged by size: 

                3830    2686    1935
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     443     802    1478    2866    3048    4545    4915    7662 
   Size:        443     359     676    1388     182    1497     370    2747

Cuts at:   7662    7761    8451 
   Size:         99     690

  Fragments arranged by size: 

                2747    1497    1388     690     676     443     370     359
                 182      99
 
PfoI T'CCnGG_A

Cuts at:      0     275     685     801    2865    4250    5100    7160    8451 
   Size:        275     410     116    2064    1385     850    2060    1291

  Fragments arranged by size: 

                2064    2060    1385    1291     850     410     275     116
            
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1439    1471    2427    2459    2656    2688    2847    2879 
   Size:       1439      32     956      32     197      32     159      32

Cuts at:   2879    8197    8229    8451 
   Size:       5318      32     222

  Fragments arranged by size: 

                5318    1439     956     222     197     159      32      32
                  32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     367    2620    3856    5413    7264    7758    7905    8058 
   Size:        367    2253    1236    1557    1851     494     147     153

Cuts at:   8058    8451 
   Size:        393

  Fragments arranged by size: 

                2253    1851    1557    1236     494     393     367     153
                 147
 
PshAI GACnn'nnGTC

Cuts at:      0    8063    8451 
   Size:       8063     388
 
PspOMI G'GGCC_C

Cuts at:      0    3620    8451 
   Size:       3620    4831
 
PspXI vC'TCGA_Gb

Cuts at:      0    7795    8451 
   Size:       7795     656
 
PstI C_TGCA'G

Cuts at:      0    1437    2562    2766    3178    3566    7619    7634    7775 
   Size:       1437    1125     204     412     388    4053      15     141

Cuts at:   7775    8451 
   Size:        676

  Fragments arranged by size: 

                4053    1437    1125     676     412     388     204     141
                  15
 
PvuII CAG'CTG

Cuts at:      0    1196    1546    1771    2908    3503    3628    4694    5423 
   Size:       1196     350     225    1137     595     125    1066     729

Cuts at:   5423    5922    6980    8013    8451 
   Size:        499    1058    1033     438

  Fragments arranged by size: 

                1196    1137    1066    1058    1033     729     595     499
                 438     350     225     125
 
RsrII CG'GwC_CG

Cuts at:      0    5636    8451 
   Size:       5636    2815
 
SacI G_AGCT'C

Cuts at:      0     576    3584    3602    4660    4901    7604    7711    7931 
   Size:        576    3008      18    1058     241    2703     107     220

Cuts at:   7931    8451 
   Size:        520

  Fragments arranged by size: 

                3008    2703    1058     576     520     241     220     107
                  18
 
SacII CC_GC'GG

Cuts at:      0     570    8451 
   Size:        570    7881
 
SanDI GG'GwC_CC

Cuts at:      0    2620    5413    8451 
   Size:       2620    2793    3038

  Fragments arranged by size: 

                3038    2793    2620
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2840    4376    4889    5287    8451 
   Size:       2840    1536     513     398    3164

  Fragments arranged by size: 

                3164    2840    1536     513     398
 
ScaI AGT'ACT

Cuts at:      0     138    2349    3903    3939    4774    8451 
   Size:        138    2211    1554      36     835    3677

  Fragments arranged by size: 

                3677    2211    1554     835     138      36
 
SexAI A'CCwGG_T

Cuts at:      0    7538    8451 
   Size:       7538     913
 
SfcI C'TryA_G

Cuts at:      0    1104    1433    2462    2558    2762    3174    3406    3562 
   Size:       1104     329    1029      96     204     412     232     156

Cuts at:   3562    4106    4533    5424    6572    7615    7630    7771    8451 
   Size:        544     427     891    1148    1043      15     141     680

  Fragments arranged by size: 

                1148    1104    1043    1029     891     680     544     427
                 412     329     232     204     156     141      96      15
            
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    7867    8451 
   Size:       7867     584
 
SmlI C'TyrA_G

Cuts at:      0     871    2266    2971    3026    6049    6543    7795    7993 
   Size:        871    1395     705      55    3023     494    1252     198

Cuts at:   7993    8451 
   Size:        458

  Fragments arranged by size: 

                3023    1395    1252     871     705     494     458     198
                  55
 
SpeI A'CTAG_T

Cuts at:      0    3232    8451 
   Size:       3232    5219
 
SspI AAT'ATT

Cuts at:      0    1536    5678    6491    8451 
   Size:       1536    4142     813    1960

  Fragments arranged by size: 

                4142    1960    1536     813
 
StuI AGG'CCT

Cuts at:      0    1241    2537    3823    8451 
   Size:       1241    1296    1286    4628

  Fragments arranged by size: 

                4628    1296    1286    1241
 
StyI C'CwwG_G

Cuts at:      0     620    2390    2918    3095    3509    3575    4539    5621 
   Size:        620    1770     528     177     414      66     964    1082

Cuts at:   5621    6359    6680    8451 
   Size:        738     321    1771

  Fragments arranged by size: 

                1771    1770    1082     964     738     620     528     414
                 321     177      66
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     809    1965    3349    3502    4353    4460    4922    5624 
   Size:        809    1156    1384     153     851     107     462     702

Cuts at:   5624    7369    8451 
   Size:       1745    1082

  Fragments arranged by size: 

                1745    1384    1156    1082     851     809     702     462
                 153     107
 
TatI w'GTAC_w

Cuts at:      0     130     136    1507    2347    3901    3937    4180    4293 
   Size:        130       6    1371     840    1554      36     243     113

Cuts at:   4293    4772    5041    6068    6815    8451 
   Size:        479     269    1027     747    1636

  Fragments arranged by size: 

                1636    1554    1371    1027     840     747     479     269
                 243     130     113      36       6
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1316    1348    3100    3132    3539    3571    5108    5140 
   Size:       1316      32    1752      32     407      32    1537      32

Cuts at:   5140    6648    6680    8451 
   Size:       1508      32    1771

  Fragments arranged by size: 

                1771    1752    1537    1508    1316     407      32      32
                  32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1348    8451 
   Size:       1348    7103
 
XbaI T'CTAG_A

Cuts at:      0    4818    4869    8451 
   Size:       4818      51    3582

  Fragments arranged by size: 

                4818    3582      51
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1378    3762    3849    4347    5406    8451 
   Size:       1378    2384      87     498    1059    3045

  Fragments arranged by size: 

                3045    2384    1378    1059     498      87
 
XhoI C'TCGA_G

Cuts at:      0    7795    8451 
   Size:       7795     656
 
XmnI GAAnn'nnTTC

Cuts at:      0     263    5443    7037    7103    8451 
   Size:        263    5180    1594      66    1348

  Fragments arranged by size: 

                5180    1594    1348     263      66
 

 Enzymes that do cut:

     AccI     AclI     AcuI     AfeI    AflII   AflIII     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI     AvaI
     BaeI    BamHI     BanI    BanII     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI    CspCI     DraI   DraIII     DrdI     EaeI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI    HaeII
    Hin4I   HincII  HindIII     HpaI     MmeI     MscI     MslI   MspA1I
     NcoI     NdeI     NsiI     NspI     PasI     PciI    PflMI     PfoI
     PpiI    PpuMI    PshAI   PspOMI    PspXI     PstI    PvuII    RsrII
     SacI    SacII    SanDI     SapI     ScaI    SexAI     SfcI     SfiI
     SmlI     SpeI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AgeI     AscI    AsiSI    AvrII     BbeI
     BmtI    BsiEI    BsiWI    BsrFI   BssHII  BstZ17I     ClaI     EagI
     FseI     FspI    FspAI     KasI     KpnI     MfeI     MluI     NaeI
     NarI   NgoMIV     NheI     NotI     NruI     PacI     PmeI     PmlI
     PsiI     PsrI     PvuI     SalI     SbfI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SphI     SrfI     SwaI     XmaI     ZraI
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