Restriction map for pF1KE0477
##################################
# NO CUT ENZYMES (  158 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
AspEI       AspHI       AvaI        AvrII       BalI        
BamHI       BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsiEI       
BsiHKAI     BsiWI       BsmAI       BsmBI       BsoBI       
Bsp1407I    BspDI       BspEI       BspLU11I    BspMI       
BsrBI       BsrFI       BsrGI       BssSI       Bst1107I    
BstAPI      BstBI       BstXI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoT22I     FauI        FbaI        
FseI        HaeII       HgaI        Hin1I       HincII      
HindII      HindIII     HpaI        KasI        KspI        
MaeI        MamI        MfeI        MluI        MluNI       
MroI        MscI        MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       Psp1406I    
PstI        PvuI        PvuII       RsrII       SacI        
SacII       SalI        SbfI        ScaI        SexAI       
SfaNI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TaqI        TthHB8I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AlwNI          38  CAGCCGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AseI          522  ATTAAT               AT^TAAT
AsnI          522  ATTAAT               AT^TAAT
AspI           75  GACCGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII          23  TGCGCA               TGC^GCA
BmyI           32  GTGCCC               G[AGT]GC[ACT]^C
BsmI          230  GCATTC               GAATGC(1/-1)
Bsp1286I       32  GTGCCC               G[AGT]GC[ACT]^C
BsrDI         743  CATTGC               GCAATG(2/0)
BssHII          5  GCGCGC               G^CGCGC
BstEII        598  GGTTACC              G^GT[ACGT]ACC
BstF5I        366  GGATG                GGATG(2/0)
BstNI         603  CCTGG                CC^[AT]GG
BstPI         598  GGTTACC              G^GT[ACGT]ACC
BstYI         473  AGATCC               [GA]^GATC[CT]
EaeI          120  CGGCCA               [CT]^GGCC[GA]
EcoO65I       598  GGTTACC              G^GT[ACGT]ACC
EcoRI         682  GAATTC               G^AATTC
EcoRII        603  CCTGG                ^CC[AT]GG
EcoRV         795  GATATC               GAT^ATC
FokI          366  GGATG                GGATG(9/13)
FspI           23  TGCGCA               TGC^GCA
MaeII         788  ACGT                 A^CGT
MflI          473  AGATCC               [GA]^GATC[CT]
MspA1I         41  CCGCTG               C[AC]G^C[GT]G
MvaI          603  CCTGG                CC^[AT]GG
PflFI          75  GACCGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshBI         522  ATTAAT               AT^TAAT
SapI          886  GAAGAGC              GCTCTTC(1/4)
SmlI          821  CTCAAG               C^T[CT][GA]AG
Tsp45I        170  GTCAC                ^GT[GC]AC
Tth111I        75  GACCGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoII         473  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

Acc65I        427  GGTACC               G^GTACC
              639  GGTACC              
AcsI          482  GAATTT               [GA]^AATT[CT]
              682  GAATTC              
AlwI          474  GATCC                GGATC(4/5)
              544  GATCC               
ApoI          482  GAATTT               [GA]^AATT[CT]
              682  GAATTC              
Asp700I       390  GAATATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              457  GAAGTTATTC          
Asp718I       427  GGTACC               G^GTACC
              639  GGTACC              
AvaII          62  GGACC                G^G[AT]CC
               74  GGACC               
BcnI           77  CCGGG                CC^[GC]GG
              595  CCGGG               
BseRI         112  GAGGAG               GAGGAG(10/8)
              767  GAGGAG              
BsgI           68  GTGCAG               GTGCAG(16/14)
              790  GTGCAG              
BsmFI          61  GGGAC                GGGAC(10/14)
               73  GGGAC               
BspHI         189  TCATGA               T^CATGA
              564  TCATGA              
DdeI          110  CTGAG                C^T[ACGT]AG
              437  CTCAG               
EarI          148  CTCTTC               CTCTTC(1/4)
              886  GAAGAG              
HaeIII         96  GGCC                 GG^CC
              121  GGCC                
HapII          77  CCGG                 C^CGG
              595  CCGG                
HpaII          77  CCGG                 C^CGG
              595  CCGG                
KpnI          427  GGTACC               GGTAC^C
              639  GGTACC              
Ksp632I       148  CTCTTC               CTCTTC(1/4)
              886  GAAGAG              
MslI          231  CATTCCTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              783  CACAAACGTG          
MspI           77  CCGG                 C^CGG
              595  CCGG                
NciI           77  CCGGG                CC^[GC]GG
              595  CCGGG               
RcaI          189  TCATGA               T^CATGA
              564  TCATGA              
TspRI          66  CAGTG                CA[GC]TG[ACGT][ACGT]^
              108  CACTG               
XmnI          390  GAATATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              457  GAAGTTATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   42 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        BanI        
BbvI        BsaJI       BsiYI       BslI        BsrI        
BssKI       BstUI       Cac8I       CfoI        Cfr13I      
DpnII       EcoT14I     Fnu4HI      HhaI        HinP1I      
HinfI       HphI        ItaI        MaeIII      MboI        
MboII       MnlI        MseI        MvnI        MwoI        
NdeII       NlaIII      NlaIV       RsaI        Sau3AI      
Sau96I      ScrFI       StyI        TfiI        Tru9I       
TseI        Tsp509I     
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