Restriction map for pF1KE0477
 (Linear) (Six-base) MAPSORT of: KIEE0477.seq  Check: 5793  from: 1  to: 915



KIEE0477.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

Acc65I G'GTAC_C

Cuts at:      0     427     639     915 
   Size:        427     212     276

  Fragments arranged by size: 

                 427     276     212
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     223     569     915 
   Size:        223     346     346

  Fragments arranged by size: 

                 346     346     223
 
AleI CACnn'nnGTG

Cuts at:      0     787     915 
   Size:        787     128
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     805     915 
   Size:        805     110
 
AlwNI CAG_nnn'CTG

Cuts at:      0      43     915 
   Size:         43     872
 
ApoI r'AATT_y

Cuts at:      0     482     682     915 
   Size:        482     200     233

  Fragments arranged by size: 

                 482     233     200
 
AseI AT'TA_AT

Cuts at:      0     523     915 
   Size:        523     392
 
BanI G'GyrC_C

Cuts at:      0      31     427     639     915 
   Size:         31     396     212     276

  Fragments arranged by size: 

                 396     276     212      31
 
Bme1580I G_kGCm'C

Cuts at:      0      36     915 
   Size:         36     879
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     806     915 
   Size:        806     109
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     127     782     915 
   Size:        127     655     133

  Fragments arranged by size: 

                 655     133     127
 
BseYI C'CCAG_C

Cuts at:      0      36     915 
   Size:         36     879
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      89     811     915 
   Size:         89     722     104

  Fragments arranged by size: 

                 722     104      89
 
BsmI GAATG_Cn'

Cuts at:      0     230     915 
   Size:        230     685
 
Bsp1286I G_dGCh'C

Cuts at:      0      36     915 
   Size:         36     879
 
BspHI T'CATG_A

Cuts at:      0     189     564     915 
   Size:        189     375     351

  Fragments arranged by size: 

                 375     351     189
 
BsrDI GCAATG_nn'

Cuts at:      0     742     915 
   Size:        742     173
 
BssHII G'CGCG_C

Cuts at:      0       5     915 
   Size:          5     910
 
BstEII G'GTnAC_C

Cuts at:      0     598     915 
   Size:        598     317
 
BstYI r'GATC_y

Cuts at:      0     473     915 
   Size:        473     442
 
EaeI y'GGCC_r

Cuts at:      0     120     915 
   Size:        120     795
 
EarI CTCTTCn'nnn_

Cuts at:      0     154     881     915 
   Size:        154     727      34

  Fragments arranged by size: 

                 727     154      34
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     223     569     915 
   Size:        223     346     346

  Fragments arranged by size: 

                 346     346     223
 
EcoRI G'AATT_C

Cuts at:      0     682     915 
   Size:        682     233
 
EcoRV GAT'ATC

Cuts at:      0     797     915 
   Size:        797     118
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     631     763     915 
   Size:        631     132     152

  Fragments arranged by size: 

                 631     152     132
 
FspI TGC'GCA

Cuts at:      0      25     915 
   Size:         25     890
 
KpnI G_GTAC'C

Cuts at:      0     431     643     915 
   Size:        431     212     272

  Fragments arranged by size: 

                 431     272     212
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     715     915 
   Size:        715     200
 
MslI CAynn'nnrTG

Cuts at:      0     235     787     915 
   Size:        235     552     128

  Fragments arranged by size: 

                 552     235     128
 
MspA1I CmG'CkG

Cuts at:      0      43     915 
   Size:         43     872
 
SapI GCTCTTCn'nnn_

Cuts at:      0     881     915 
   Size:        881      34
 
SmlI C'TyrA_G

Cuts at:      0     821     915 
   Size:        821      94
 
StyI C'CwwG_G

Cuts at:      0     329     477     906     915 
   Size:        329     148     429       9

  Fragments arranged by size: 

                 429     329     148       9
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     163     195     915 
   Size:        163      32     720

  Fragments arranged by size: 

                 720     163      32
 
Tth111I GACn'n_nGTC

Cuts at:      0      78     915 
   Size:         78     837
 
XmnI GAAnn'nnTTC

Cuts at:      0     394     461     915 
   Size:        394      67     454

  Fragments arranged by size: 

                 454     394      67
 

 Enzymes that do cut:

   Acc65I     AcuI     AleI     AlfI    AlwNI     ApoI     AseI     BanI
 Bme1580I    BpuEI    BseRI    BseYI     BsgI     BsmI Bsp1286I    BspHI
    BsrDI   BssHII   BstEII    BstYI     EaeI     EarI  Eco57MI    EcoRI
    EcoRV     FalI     FspI     KpnI     MmeI     MslI   MspA1I     SapI
     SmlI     StyI     TstI  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII
     BaeI    BamHI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspMI    BsrBI
    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoNI EcoO109I     FseI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     MfeI     MluI     MscI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI     XbaI     XcmI     XhoI     XmaI     ZraI
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