Restriction map for pF1KE0461
##################################
# NO CUT ENZYMES (  172 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AvaII       
AviII       AvrII       BamHI       BanI        BbeI        
BbrPI       BbsI        BbvCI       BclI        BcnI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaJI       BsaWI       BseAI       BsiEI       
BsiWI       BsmAI       BsmBI       BsoBI       BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BssHII      BssKI       BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstNI       BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr13I      ClaI        CpoI        DraII       
DraIII      DrdI        DsaI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRII      
EcoT14I     EcoT22I     FbaI        FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HpaI        KasI        KpnI        Ksp632I     KspI        
MaeII       MamI        MluI        MroI        MslI        
MvaI        MvnI        NarI        NciI        NcoI        
NheI        NlaIV       NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SapI        
Sau96I      SbfI        ScaI        ScrFI       SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        StyI        SwaI        Tsp45I      Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   60 enzymes) #
###################################

AflII         303  CTTAAG               C^TTAAG
AlwI          543  GGATC                GGATC(4/5)
AspHI         257  GAGCTC               G[AT]GC[AT]^C
BalI          168  TGGCCA               TGG^CCA
BanII         257  GAGCTC               G[GA]GC[CT]^C
BcgI          453  CGAAGCGAGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         195  GGATAC               GTATCC(6/5)
BfaI          281  CTAG                 C^TAG
BfrI          303  CTTAAG               C^TTAAG
BmyI          257  GAGCTC               G[AGT]GC[ACT]^C
BseRI         245  GAGGAG               GAGGAG(10/8)
BsgI            8  CTGCAC               GTGCAG(16/14)
BsiHKAI       257  GAGCTC               G[AT]GC[AT]^C
BsiYI         589  CCGCAGGGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          589  CCGCAGGGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI          53  GGGAC                GGGAC(10/14)
BsmI          549  GAATGC               GAATGC(1/-1)
Bsp1286I      257  GAGCTC               G[AGT]GC[ACT]^C
Bsp1407I      567  TGTACA               T^GTACA
BsrFI         599  GCCGGC               [GA]^CCGG[CT]
BsrGI         567  TGTACA               T^GTACA
BsrI          608  CCAGT                ACTGG(1/-1)
BstF5I        531  GGATG                GGATG(2/0)
BstYI         543  GGATCT               [GA]^GATC[CT]
Cfr10I        599  GCCGGC               [GA]^CCGG[CT]
DdeI          557  CTGAG                C^T[ACGT]AG
DpnII         544  GATC                 ^GATC
DraI           34  TTTAAA               TTT^AAA
EaeI          168  TGGCCA               [CT]^GGCC[GA]
EcoRV         274  GATATC               GAT^ATC
FokI          531  GGATG                GGATG(9/13)
HapII         600  CCGG                 C^CGG
HincII        315  GTTGAC               GT[CT]^[GA]AC
HindII        315  GTTGAC               GT[CT]^[GA]AC
HindIII       537  AAGCTT               A^AGCTT
HpaII         600  CCGG                 C^CGG
HphI          335  TCACC                GGTGA(8/7)
MaeI          281  CTAG                 C^TAG
MboI          544  GATC                 ^GATC
MfeI          128  CAATTG               C^AATTG
MflI          543  GGATCT               [GA]^GATC[CT]
MluNI         168  TGGCCA               TGG^CCA
MscI          168  TGGCCA               TGG^CCA
MspA1I        309  CAGCTG               C[AC]G^C[GT]G
MspI          600  CCGG                 C^CGG
MunI          128  CAATTG               C^AATTG
NaeI          599  GCCGGC               GCC^GGC
NdeI          404  CATATG               CA^TATG
NdeII         544  GATC                 ^GATC
NgoMIV        599  GCCGGC               G^CCGGC
NlaIII         57  CATG                 CATG^
PleI          559  GAGTC                GAGTC(4/5)
PstI          184  CTGCAG               CTGCA^G
PvuII         309  CAGCTG               CAG^CTG
SacI          257  GAGCTC               GAGCT^C
Sau3AI        544  GATC                 ^GATC
SspBI         567  TGTACA               T^GTACA
TfiI          330  GAATC                G^A[AT]TC
TspRI         609  CAGTG                CA[GC]TG[ACGT][ACGT]^
XhoII         543  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AciI          589  CCGC                 CCGC(-3/-1)
              253  GCGG                
AcsI          103  AAATTT               [GA]^AATT[CT]
              139  GAATTT              
ApoI          103  AAATTT               [GA]^AATT[CT]
              139  GAATTT              
Cac8I          77  GCTTGC               GC[ACGT]^[ACGT]GC
              599  GCCGGC              
FauI          588  CCCGC                CCCGC(4/6)
              253  GCGGG               
HaeIII        169  GGCC                 GG^CC
              598  GGCC                
HinfI         330  GAATC                G^A[ACGT]TC
              559  GAGTC               
MaeIII        226  GTTAC                ^GT[ACGT]AC
              400  GTTAC               
MseI           35  TTAA                 T^TAA
              304  TTAA                
MwoI          250  GCTGCGGGAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              635  GCTGTGGCTGC         
SfaNI         322  GATGC                GCATC(5/9)
              487  GATGC               
SfcI          184  CTGCAG               C^T[GA][CT]AG
              240  CTACAG              
SmlI          303  CTTAAG               C^T[CT][GA]AG
              496  CTTGAG              
TaqI          158  TCGA                 T^CGA
              452  TCGA                
Tru9I          35  TTAA                 T^TAA
              304  TTAA                
TthHB8I       158  TCGA                 T^CGA
              452  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   10 enzymes) #
#########################################

AfaI        AluI        BbvI        Fnu4HI      ItaI        
MboII       MnlI        RsaI        TseI        Tsp509I     
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