Restriction map for pF1KE0461
 (Circular) (Six-base) MAPSORT of: pF1KE0461.seq  Check: 9254  from: 1  to: 3758



pF1KE0461  3758 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AccI GT'mk_AC

Cuts at:    796     796 
   Size:       3758
 
Acc65I G'GTAC_C

Cuts at:    774     774 
   Size:       3758
 
AclI AA'CG_TT

Cuts at:   3598    3598 
   Size:       3758
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    557     674    1583    2015    2762    3030     557 
   Size:        117     909     432     747     268    1285

  Fragments arranged by size: 

                1285     909     747     432     268     117
 
AflII C'TTAA_G

Cuts at:    411     411 
   Size:       3758
 
AflIII A'CryG_T

Cuts at:   1110    2214    3191    1110 
   Size:       1104     977    1677

  Fragments arranged by size: 

                1677    1104     977
 
AgeI A'CCGG_T

Cuts at:   2146    2146 
   Size:       3758
 
AlwNI CAG_nnn'CTG

Cuts at:   1063    2630    1063 
   Size:       1567    2191
 
ApaLI G'TGCA_C

Cuts at:   2528    2528 
   Size:       3758
 
ApoI r'AATT_y

Cuts at:    211     247     762    3111    3367     211 
   Size:         36     515    2349     256     602

  Fragments arranged by size: 

                2349     602     515     256      36
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3758
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3758
 
AvaI C'yCGr_G

Cuts at:    778     778 
   Size:       3758
 
BamHI G'GATC_C

Cuts at:    783     783 
   Size:       3758
 
BanI G'GyrC_C

Cuts at:    774    1188    1436    1471     774 
   Size:        414     248      35    3061

  Fragments arranged by size: 

                3061     414     248      35
 
BanII G_rGCy'C

Cuts at:    369     772    1802     369 
   Size:        403    1030    2325

  Fragments arranged by size: 

                2325    1030     403
 
BbeI G_GCGC'C

Cuts at:   1192    1440    1192 
   Size:        248    3510
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    550     584     550 
   Size:         34    3724
 
BciVI GTATCCnnnnn_n'

Cuts at:    297    1656    2417    3758     297 
   Size:       1359     761    1341     297

  Fragments arranged by size: 

                1359    1341     761     297
 
BclI T'GATC_A

Cuts at:   1278    1278 
   Size:       3758
 
BglII A'GATC_T

Cuts at:   1273    3310    1273 
   Size:       2037    1721
 
BlpI GC'TnA_GC

Cuts at:    870     870 
   Size:       3758
 
Bme1580I G_kGCm'C

Cuts at:   1383    1476    2532    1383 
   Size:         93    1056    2609

  Fragments arranged by size: 

                2609    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    970    1051    1386    3530     970 
   Size:         81     335    2144    1198

  Fragments arranged by size: 

                2144    1198     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2125    2125 
   Size:       3758
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    625     934    1813    2305    2603     625 
   Size:        309     879     492     298    1780

  Fragments arranged by size: 

                1780     879     492     309     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3462    3462 
   Size:       3758
 
BsaAI yAC'GTr

Cuts at:   1111    1111 
   Size:       3758
 
BsaBI GATnn'nnATC

Cuts at:      6    1272    3309       6 
   Size:       1266    2037     455

  Fragments arranged by size: 

                2037    1266     455
 
BsaHI Gr'CG_yC

Cuts at:   1189    1437    2139    3635    1189 
   Size:        248     702    1496    1312

  Fragments arranged by size: 

                1496    1312     702     248
 
BsaWI w'CCGG_w

Cuts at:    947    1468    2146    2420    2567    2978     947 
   Size:        521     678     274     147     411    1727

  Fragments arranged by size: 

                1727     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    572     602     572 
   Size:         30    3728
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    368     368 
   Size:       3758
 
BseYI C'CCAG_C

Cuts at:   1183    1941    2518    3014    1183 
   Size:        758     577     496    1927

  Fragments arranged by size: 

                1927     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    101     101 
   Size:       3758
 
BsiEI CG_ry'CG

Cuts at:    104    1300    1346    2554     104 
   Size:       1196      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1196      46
 
BsiHKAI G_wGCw'C

Cuts at:    369     772    1550    1740    2532     369 
   Size:        403     778     190     792    1595

  Fragments arranged by size: 

                1595     792     778     403     190
 
BsmI GAATG_Cn'

Cuts at:    663     663 
   Size:       3758
 
Bsp1286I G_dGCh'C

Cuts at:    369     772    1383    1476    1550    1740    1802    2532     369 
   Size:        403     611      93      74     190      62     730    1595

  Fragments arranged by size: 

                1595     730     611     403     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   2978    2978 
   Size:       3758
 
BspHI T'CATG_A

Cuts at:   2934    2934 
   Size:       3758
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    808    1022    1324    1705     808 
   Size:        214     302     381    2861

  Fragments arranged by size: 

                2861     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2050    2104    3588    3753      50 
   Size:       2000      54    1484     165      55

  Fragments arranged by size: 

                2000    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1670    1670 
   Size:       3758
 
BsrFI r'CCGG_y

Cuts at:    707    1756    1937    2146     707 
   Size:       1049     181     209    2319

  Fragments arranged by size: 

                2319    1049     209     181
 
BsrGI T'GTAC_A

Cuts at:    675     675 
   Size:       3758
 
BssSI C'ACGA_G

Cuts at:   2029    2387    2029 
   Size:        358    3400
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1135    1135 
   Size:       3758
 
BstBI TT'CG_AA

Cuts at:   2119    2119 
   Size:       3758
 
BstYI r'GATC_y

Cuts at:      1     651     783    1250    1273    1608    1854    2866    2952 
   Size:        650     132     467      23     335     246    1012      86

Cuts at:   2952    2964    3310       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     650     467     449     346     335     246     132
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3758
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1708    1889    2052    1708 
   Size:        181     163    3414

  Fragments arranged by size: 

                3414     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1001    1001 
   Size:       3758
 
DraI TTT'AAA

Cuts at:    144     757     144 
   Size:        613    3145
 
DrdI GACnn_nn'nnGTC

Cuts at:   1464    2322    1464 
   Size:        858    2900
 
EaeI y'GGCC_r

Cuts at:    276    1343    1517    1908    1935    3280     276 
   Size:       1067     174     391      27    1345     754

  Fragments arranged by size: 

                1345    1067     754     391     174      27
 
EagI C'GGCC_G

Cuts at:   1343    1343 
   Size:       3758
 
EarI CTCTTCn'nnn_

Cuts at:   1781    1991    1781 
   Size:        210    3548
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1966    2276    2422    3568    1966 
   Size:        310     146    1146    2156

  Fragments arranged by size: 

                2156    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    367     770     367 
   Size:        403    3355
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    557     674    1583    2015    2762    3030     557 
   Size:        117     909     432     747     268    1285

  Fragments arranged by size: 

                1285     909     747     432     268     117
 
EcoO109I rG'GnC_Cy

Cuts at:    897     897 
   Size:       3758
 
EcoRI G'AATT_C

Cuts at:    762     762 
   Size:       3758
 
EcoRV GAT'ATC

Cuts at:    384     384 
   Size:       3758
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1777    1777 
   Size:       3758
 
FspI TGC'GCA

Cuts at:   1539    1539 
   Size:       3758
 
HaeII r_GCGC'y

Cuts at:   1192    1440    2462    3441    1192 
   Size:        248    1022     979    1509

  Fragments arranged by size: 

                1509    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1600    1630    1632    1662    1600 
   Size:         30       2      30    3696

  Fragments arranged by size: 

                3696      30      30       2
 
HincII GTy'rAC

Cuts at:    425     797     425 
   Size:        372    3386
 
HindIII A'AGCT_T

Cuts at:    645     645 
   Size:       3758
 
KasI G'GCGC_C

Cuts at:   1188    1436    1188 
   Size:        248    3510
 
KpnI G_GTAC'C

Cuts at:    778     778 
   Size:       3758
 
MfeI C'AATT_G

Cuts at:    236     236 
   Size:       3758
 
MluI A'CGCG_T

Cuts at:   3191    3191 
   Size:       3758
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2429    2613    2429 
   Size:        184    3574
 
MscI TGG'CCA

Cuts at:    278    1519     278 
   Size:       1241    2517
 
MslI CAynn'nnrTG

Cuts at:   1747    1874    1747 
   Size:        127    3631
 
MspA1I CmG'CkG

Cuts at:    419     869    1183    1543    2556    2801     419 
   Size:        450     314     360    1013     245    1376

  Fragments arranged by size: 

                1376    1013     450     360     314     245
 
NaeI GCC'GGC

Cuts at:    709    1939     709 
   Size:       1230    2528
 
NarI GG'CG_CC

Cuts at:   1189    1437    1189 
   Size:        248    3510
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3758
 
NdeI CA'TA_TG

Cuts at:    513     513 
   Size:       3758
 
NgoMIV G'CCGG_C

Cuts at:    707    1937     707 
   Size:       1230    2528
 
NspI r_CATG'y

Cuts at:    811    1311    2218     811 
   Size:        500     907    2351

  Fragments arranged by size: 

                2351     907     500
 
PciI A'CATG_T

Cuts at:   2214    2214 
   Size:       3758
 
PmeI GTTT'AAAC

Cuts at:    757     757 
   Size:       3758
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2897    2929    2897 
   Size:         32    3726
 
PstI C_TGCA'G

Cuts at:    296     805    1490     296 
   Size:        509     685    2564

  Fragments arranged by size: 

                2564     685     509
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3758
 
PvuII CAG'CTG

Cuts at:    419    1183    1543     419 
   Size:        764     360    2634

  Fragments arranged by size: 

                2634     764     360
 
RsrII CG'GwC_CG

Cuts at:   1953    1953 
   Size:       3758
 
SacI G_AGCT'C

Cuts at:    369     772     369 
   Size:        403    3355
 
SalI G'TCGA_C

Cuts at:    795     795 
   Size:       3758
 
SapI GCTCTTCn'nnn_

Cuts at:   1781    1991    1781 
   Size:        210    3548
 
SbfI CC_TGCA'GG

Cuts at:    805     805 
   Size:       3758
 
ScaI AGT'ACT

Cuts at:   3275    3275 
   Size:       3758
 
SfcI C'TryA_G

Cuts at:     33     292     348     801    1486    2479    2670      33 
   Size:        259      56     453     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     453     259     191      56
 
SfoI GGC'GCC

Cuts at:   1190    1438    1190 
   Size:        248    3510
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3758
 
SmaI CCC'GGG

Cuts at:    780     780 
   Size:       3758
 
SmlI C'TyrA_G

Cuts at:    411     604     913    1828    2320    2582     411 
   Size:        193     309     915     492     262    1587

  Fragments arranged by size: 

                1587     915     492     309     262     193
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3758
 
SphI G_CATG'C

Cuts at:    811    1311     811 
   Size:        500    3258
 
StyI C'CwwG_G

Cuts at:    107     892    1246     107 
   Size:        785     354    2619

  Fragments arranged by size: 

                2619     785     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1341    1474    1933    2142    1341 
   Size:        133     459     209    2957

  Fragments arranged by size: 

                2957     459     209     133
 
TatI w'GTAC_w

Cuts at:    397     675    3273     397 
   Size:        278    2598     882

  Fragments arranged by size: 

                2598     882     278
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3275    3307    3275 
   Size:         32    3726
 
Tth111I GACn'n_nGTC

Cuts at:   1555    1555 
   Size:       3758
 
XbaI T'CTAG_A

Cuts at:    789     789 
   Size:       3758
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1253    1253 
   Size:       3758
 
XmaI C'CCGG_G

Cuts at:    778     778 
   Size:       3758
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BcgI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI
     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI
    BstBI    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV
     FalI     FspI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NspI     PciI     PmeI     PpiI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI     BbsI    BbvCI    BfrBI     BglI    BmgBI
     BmtI     BpmI   Bpu10I    BsiWI    BsmBI   BssHII   BstEII    BstXI
  BstZ17I   Bsu36I     ClaI    CspCI   DraIII    EcoNI     FseI    FspAI
     HpaI     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PfoI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI
     StuI     SwaI     XhoI     XmnI     ZraI
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