Restriction map for pF1KE0459
##################################
# NO CUT ENZYMES (  168 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmBI       BsoBI       Bsp1407I    BspDI       
BspEI       BspLU11I    BsrBI       BsrDI       BsrGI       
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstXI       BstZ17I     CelII       ClaI        
CpoI        DraI        DraIII      DrdI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT14I     FbaI        FseI        FspI        Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        Ksp632I     MaeI        MaeII       MamI        
MboII       MfeI        MluI        MluNI       MroI        
MscI        MseI        MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RsaI        RsrII       SacI        SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        Tru9I       Tsp509I     
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AlwI          370  GGATC                GGATC(4/5)
AlwNI          71  CAGGCGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI           68  CCTCAGG              CC^T[ACGT]AGG
BanII         109  GGGCTC               G[GA]GC[CT]^C
BmyI          109  GGGCTC               G[AGT]GC[ACT]^C
BsaI          315  GAGACC               GGTCTC(1/5)
BsmAI         315  GAGAC                GTCTC(1/5)
BsmI          235  GAATGC               GAATGC(1/-1)
Bsp1286I      109  GGGCTC               G[AGT]GC[ACT]^C
BspHI          48  TCATGA               T^CATGA
BspMI         116  GCAGGT               ACCTGC(4/8)
BssHII        137  GCGCGC               G^CGCGC
BstYI         370  GGATCT               [GA]^GATC[CT]
Bsu36I         68  CCTCAGG              CC^T[ACGT]AGG
DpnII         371  GATC                 ^GATC
DsaI           28  CCGCGG               C^C[GA][CT]GG
Eco81I         68  CCTCAGG              CC^T[ACGT]AGG
EcoT22I       343  ATGCAT               ATGCA^T
HaeII          73  GGCGCT               [GA]GCGC^[CT]
HinfI         248  GATTC                G^A[ACGT]TC
KspI           28  CCGCGG               CCGC^GG
MaeIII         38  GTCAC                ^GT[ACGT]AC
MboI          371  GATC                 ^GATC
MflI          370  GGATCT               [GA]^GATC[CT]
MslI           43  CATCATCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I         28  CCGCGG               C[AC]G^C[GT]G
NdeII         371  GATC                 ^GATC
NsiI          343  ATGCAT               ATGCA^T
NspI          345  GCATGC               [GA]CATG^[CT]
PpuMI         299  GGGTCCC              [GA]G^G[AT]CC[CT]
RcaI           48  TCATGA               T^CATGA
SacII          28  CCGCGG               CCGC^GG
Sau3AI        371  GATC                 ^GATC
SphI          345  GCATGC               GCATG^C
TaqI          202  TCGA                 T^CGA
TfiI          248  GATTC                G^A[AT]TC
Tsp45I         38  GTCAC                ^GT[GC]AC
TthHB8I       202  TCGA                 T^CGA
XhoII         370  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AluI          409  AGCT                 AG^CT
              433  AGCT                
BcnI           83  CCGGG                CC^[GC]GG
               91  CCGGG               
BpmI          411  CTCCAG               CTGGAG(16/14)
               59  CTGGAG              
BsrFI          11  GCCGGT               [GA]^CCGG[CT]
              437  ACCGGC              
BsrI           58  ACTGG                ACTGG(1/-1)
              296  ACTGG               
BstF5I        144  GGATG                GGATG(2/0)
              341  GGATG               
Cfr10I         11  GCCGGT               [GA]^CCGG[CT]
              437  ACCGGC              
DdeI           69  CTCAG                C^T[ACGT]AG
              313  CTGAG               
DraII         299  GGGTCCC              [GA]G^G[ACGT]CC[CT]
              415  AGGCCCC             
EcoO109I      299  GGGTCCC              [GA]G^G[ACGT]CC[CT]
              415  AGGCCCC             
FauI           27  CCCGC                CCCGC(4/6)
               97  GCGGG               
FokI          144  GGATG                GGATG(9/13)
              341  GGATG               
HgaI          104  GCGTC                GACGC(5/10)
              452  GCGTC               
HphI          378  GGTGA                GGTGA(8/7)
               39  TCACC               
NciI           83  CCGGG                CC^[GC]GG
               91  CCGGG               
NlaIII         49  CATG                 CATG^
              346  CATG                
PstI          114  CTGCAG               CTGCA^G
              445  CTGCAG              
SfaNI         204  GATGC                GCATC(5/9)
              342  GATGC               
SfcI          114  CTGCAG               C^T[GA][CT]AG
              445  CTGCAG              
TspRI          57  CACTG                CA[GC]TG[ACGT][ACGT]^
              287  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   31 enzymes) #
#########################################

AccII       AciI        AvaII       BbvI        BsaJI       
BsiYI       BslI        BsmFI       BssKI       BstNI       
BstUI       Cac8I       CfoI        Cfr13I      EcoRII      
Fnu4HI      HaeIII      HapII       HhaI        HinP1I      
HpaII       ItaI        MnlI        MspI        MvaI        
MvnI        MwoI        NlaIV       Sau96I      ScrFI       
TseI        
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