Restriction map for pF1KE0440
##################################
# NO CUT ENZYMES (  178 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AciI        
AclI        AcyI        AflII       AflIII      AgeI        
Alw44I      AlwNI       Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspHI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbvCI       BciVI       BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsaJI       BsaWI       BseAI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1286I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrFI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstYI       BstZ17I     CelII       CfoI        Cfr10I      
Cfr13I      ClaI        CpoI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        EclXI       
Eco47III    Eco52I      EcoO109I    EcoO65I     EcoRI       
EcoT14I     FauI        FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HindIII     HpaII       KasI        KpnI        KspI        
MaeI        MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MslI        MspI        
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NlaIV       
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        Sau96I      SbfI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspI        StuI        StyI        SwaI        
Tth111I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccI          490  GTAGAC               GT^[AC][GT]AC
AhdI          384  GACTTCACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI          128  GATCC                GGATC(4/5)
AocI          309  CCTTAGG              CC^T[ACGT]AGG
Asp700I        74  GAAATTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         384  GACTTCACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbsI          377  GAAGAC               GAAGAC(2/6)
BbvI          901  GCTGC                GCAGC(8/12)
BcgI           12  GCATCAGAATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI         377  GAAGAC               GAAGAC(2/6)
BseRI         100  GAGGAG               GAGGAG(10/8)
BsmAI         497  GAGAC                GTCTC(1/5)
Bsp1407I      520  TGTACA               T^GTACA
BspMI         897  ACCTGC               ACCTGC(4/8)
BsrGI         520  TGTACA               T^GTACA
BssKI          37  CCAGG                ^CC[ACGT]GG
BstNI          37  CCAGG                CC^[AT]GG
Bsu36I        309  CCTTAGG              CC^T[ACGT]AGG
Cac8I         435  GCCAGC               GC[ACGT]^[ACGT]GC
DraI          480  TTTAAA               TTT^AAA
Eam1105I      384  GACTTCACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        309  CCTTAGG              CC^T[ACGT]AGG
EcoRII         37  CCAGG                ^CC[AT]GG
EcoRV         262  GATATC               GAT^ATC
EcoT22I        65  ATGCAT               ATGCA^T
Fnu4HI        901  GCTGC                GC^[ACGT]GC
HaeIII        561  GGCC                 GG^CC
HincII        274  GTTAAC               GT[CT]^[GA]AC
HindII        274  GTTAAC               GT[CT]^[GA]AC
HpaI          274  GTTAAC               GTT^AAC
HphI          895  TCACC                GGTGA(8/7)
ItaI          901  GCTGC                GC^[ACGT]GC
MvaI           37  CCAGG                CC^[AT]GG
NlaIII         64  CATG                 CATG^
NsiI           65  ATGCAT               ATGCA^T
NspI           63  ACATGC               [GA]CATG^[CT]
ScaI          242  AGTACT               AGT^ACT
ScrFI          37  CCAGG                CC^[ACGT]GG
SfcI          662  CTGTAG               C^T[GA][CT]AG
SspBI         520  TGTACA               T^GTACA
TaqI          126  TCGA                 T^CGA
TseI          901  GCTGC                G^C[AT]GC
TthHB8I       126  TCGA                 T^CGA
XmnI           74  GAAATTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AcsI          323  AAATTC               [GA]^AATT[CT]
              428  AAATTT              
ApoI          323  AAATTC               [GA]^AATT[CT]
              428  AAATTT              
BclI          291  TGATCA               T^GATCA
              864  TGATCA              
BstF5I        105  GGATG                GGATG(2/0)
              738  GGATG               
FbaI          291  TGATCA               T^GATCA
              864  TGATCA              
FokI          105  GGATG                GGATG(9/13)
              738  GGATG               
MaeII         390  ACGT                 A^CGT
              612  ACGT                
MaeIII        461  GTGAC                ^GT[ACGT]AC
              894  GTCAC               
MspA1I        467  CAGCTG               C[AC]G^C[GT]G
              659  CAGCTG              
MwoI          562  GCCTTAACAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              721  GCAATTTTAGC         
PleI          463  GACTC                GAGTC(4/5)
              763  GACTC               
PvuII         467  CAGCTG               CAG^CTG
              659  CAGCTG              
SfaNI         106  GATGC                GCATC(5/9)
               12  GCATC               
Tsp45I        461  GTGAC                ^GT[GC]AC
              894  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   22 enzymes) #
#########################################

AfaI        AluI        BsiYI       BslI        BsrI        
DdeI        DpnII       EarI        EcoNI       HinfI       
Ksp632I     MboI        MboII       MnlI        MseI        
NdeII       RsaI        Sau3AI      TfiI        Tru9I       
Tsp509I     TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com