Restriction map for pF1KE0439
 (Linear) (Six-base) MAPSORT of: KIEE0439.seq  Check: 3585  from: 1  to: 942



KIEE0439.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AccI GT'mk_AC

Cuts at:      0     167     593     942 
   Size:        167     426     349

  Fragments arranged by size: 

                 426     349     167
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     391     942 
   Size:        391     551
 
AleI CACnn'nnGTG

Cuts at:      0     157     942 
   Size:        157     785
 
AlwNI CAG_nnn'CTG

Cuts at:      0     145     526     942 
   Size:        145     381     416

  Fragments arranged by size: 

                 416     381     145
 
ApaI G_GGCC'C

Cuts at:      0     772     942 
   Size:        772     170
 
ApoI r'AATT_y

Cuts at:      0     271     942 
   Size:        271     671
 
AvrII C'CTAG_G

Cuts at:      0     609     942 
   Size:        609     333
 
BanI G'GyrC_C

Cuts at:      0     115     942 
   Size:        115     827
 
BanII G_rGCy'C

Cuts at:      0     389     772     942 
   Size:        389     383     170

  Fragments arranged by size: 

                 389     383     170
 
BbeI G_GCGC'C

Cuts at:      0     119     942 
   Size:        119     823
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     289     580     731     919     942 
   Size:        289     291     151     188      23

  Fragments arranged by size: 

                 291     289     188     151      23
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     795     942 
   Size:        795     147
 
Bme1580I G_kGCm'C

Cuts at:      0     772     942 
   Size:        772     170
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     514     942 
   Size:        514     428
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     938     942 
   Size:        938       4
 
Bpu10I CC'TnA_GC

Cuts at:      0     289     942 
   Size:        289     653
 
BsaAI yAC'GTr

Cuts at:      0      87     942 
   Size:         87     855
 
BsaHI Gr'CG_yC

Cuts at:      0     116     942 
   Size:        116     826
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     430     460     942 
   Size:        430      30     482

  Fragments arranged by size: 

                 482     430      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      30     942 
   Size:         30     912
 
BseYI C'CCAG_C

Cuts at:      0     865     942 
   Size:        865      77
 
BsiHKAI G_wGCw'C

Cuts at:      0     295     389     942 
   Size:        295      94     553

  Fragments arranged by size: 

                 553     295      94
 
Bsp1286I G_dGCh'C

Cuts at:      0     295     389     772     942 
   Size:        295      94     383     170

  Fragments arranged by size: 

                 383     295     170      94
 
BspHI T'CATG_A

Cuts at:      0     530     942 
   Size:        530     412
 
BsrDI GCAATG_nn'

Cuts at:      0     122     942 
   Size:        122     820
 
BsrFI r'CCGG_y

Cuts at:      0     118     262     754     942 
   Size:        118     144     492     188

  Fragments arranged by size: 

                 492     188     144     118
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      24     942 
   Size:         24     918
 
BstEII G'GTnAC_C

Cuts at:      0     757     942 
   Size:        757     185
 
Bsu36I CC'TnA_GG

Cuts at:      0     220     257     942 
   Size:        220      37     685

  Fragments arranged by size: 

                 685     220      37
 
BtgI C'CryG_G

Cuts at:      0     764     942 
   Size:        764     178
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     281     942 
   Size:        281     661
 
BtsI GCAGTG_nn'

Cuts at:      0     734     942 
   Size:        734     208
 
EaeI y'GGCC_r

Cuts at:      0      62     942 
   Size:         62     880
 
EarI CTCTTCn'nnn_

Cuts at:      0     394     942 
   Size:        394     548
 
EcoICRI GAG'CTC

Cuts at:      0     387     942 
   Size:        387     555
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     391     938     942 
   Size:        391     547       4

  Fragments arranged by size: 

                 547     391       4
 
HaeII r_GCGC'y

Cuts at:      0     119     942 
   Size:        119     823
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     304     336     942 
   Size:        304      32     606

  Fragments arranged by size: 

                 606     304      32
 
KasI G'GCGC_C

Cuts at:      0     115     942 
   Size:        115     827
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     774     942 
   Size:        774     168
 
MslI CAynn'nnrTG

Cuts at:      0     157     739     942 
   Size:        157     582     203

  Fragments arranged by size: 

                 582     203     157
 
NaeI GCC'GGC

Cuts at:      0     120     264     942 
   Size:        120     144     678

  Fragments arranged by size: 

                 678     144     120
 
NarI GG'CG_CC

Cuts at:      0     116     942 
   Size:        116     826
 
NcoI C'CATG_G

Cuts at:      0     764     942 
   Size:        764     178
 
NgoMIV G'CCGG_C

Cuts at:      0     118     262     942 
   Size:        118     144     680

  Fragments arranged by size: 

                 680     144     118
 
PmlI CAC'GTG

Cuts at:      0      87     942 
   Size:         87     855
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     886     918     942 
   Size:        886      32      24

  Fragments arranged by size: 

                 886      32      24
 
PspOMI G'GGCC_C

Cuts at:      0     768     942 
   Size:        768     174
 
PstI C_TGCA'G

Cuts at:      0     141     942 
   Size:        141     801
 
SacI G_AGCT'C

Cuts at:      0     389     942 
   Size:        389     553
 
SapI GCTCTTCn'nnn_

Cuts at:      0     394     942 
   Size:        394     548
 
SfcI C'TryA_G

Cuts at:      0     137     942 
   Size:        137     805
 
SfoI GGC'GCC

Cuts at:      0     117     942 
   Size:        117     825
 
StyI C'CwwG_G

Cuts at:      0     609     764     942 
   Size:        609     155     178

  Fragments arranged by size: 

                 609     178     155
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     506     815     942 
   Size:        506     309     127

  Fragments arranged by size: 

                 506     309     127
 
XbaI T'CTAG_A

Cuts at:      0     783     942 
   Size:        783     159
 
XmnI GAAnn'nnTTC

Cuts at:      0     901     942 
   Size:        901      41
 

 Enzymes that do cut:

     AccI     AcuI     AleI    AlwNI     ApaI     ApoI    AvrII     BanI
    BanII     BbeI     BbsI    BciVI Bme1580I     BmrI     BpmI   Bpu10I
    BsaAI    BsaHI    BsaXI    BseRI    BseYI  BsiHKAI Bsp1286I    BspHI
    BsrDI    BsrFI   BstAPI   BstEII   Bsu36I     BtgI    BtgZI     BtsI
     EaeI     EarI  EcoICRI  Eco57MI    HaeII    Hin4I     KasI     MmeI
     MslI     NaeI     NarI     NcoI   NgoMIV     PmlI     PpiI   PspOMI
     PstI     SacI     SapI     SfcI     SfoI     StyI    TaqII     XbaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI     AvaI
     BaeI    BamHI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmtI     BplI    BpuEI     BsaI    BsaBI    BsaWI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI
    BsrGI   BssHII    BssSI    BstBI    BstXI    BstYI  BstZ17I     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EciI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MscI   MspA1I     NdeI     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI
    PvuII    RsrII    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I     XcmI
     XhoI     XmaI     ZraI
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