Restriction map for pF1KE1978
##################################
# NO CUT ENZYMES (  100 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AvrII       BbeI        
BciVI       BfaI        BfrI        BglII       BlnI        
BsaBI       BsaHI       BseAI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstZ17I     ClaI        CpoI        DraI        
Eam1105I    Eco47III    EcoRI       EcoRV       EcoT22I     
FseI        HgaI        Hin1I       HindIII     HpaI        
KasI        KpnI        MaeI        MamI        MfeI        
MluI        MroI        MunI        NarI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SalI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        Van91I      XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   64 enzymes) #
###################################

AccII        1064  CGCG                 CG^CG
AcsI         1552  AAATTC               [GA]^AATT[CT]
ApoI         1552  AAATTC               [GA]^AATT[CT]
Asp700I      1317  GAAGCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1606  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         716  TGCGCA               TGC^GCA
BamHI         950  GGATCC               G^GATCC
BbrPI        1039  CACGTG               CAC^GTG
BbsI          480  GAAGAC               GAAGAC(2/6)
BbvCI        1341  GCTGAGG              CCTCAGC(-5/-2)
BcgI         1168  CGAGACCCATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         1491  TGATCA               T^GATCA
BglI          161  GCCGCTCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         1196  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1196  GCTCAGC              GC^T[ACGT]AGC
BpuAI         480  GAAGAC               GAAGAC(2/6)
BsaAI        1039  CACGTG               [CT]AC^GT[GA]
BsaI         1169  GAGACC               GGTCTC(1/5)
BsaWI        1646  ACCGGA               [AT]^CCGG[AT]
BsiEI        1135  CGGCCG               CG[GA][CT]^CG
BsmAI        1169  GAGAC                GTCTC(1/5)
BsrBI         162  CCGCTC               CCGCTC(-3/-3)
BsrDI         422  GCAATG               GCAATG(2/0)
BstAPI       1038  GCACGTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI        1064  CGCG                 CG^CG
BstXI         291  CCAGGGCTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         950  GGATCC               [GA]^GATC[CT]
CelII        1196  GCTCAGC              GC^T[ACGT]AGC
DraIII       1353  CACCGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          382  GACTGGAAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1135  CGGCCG               C^GGCCG
EclXI        1135  CGGCCG               C^GGCCG
Eco52I       1135  CGGCCG               C^GGCCG
FbaI         1491  TGATCA               T^GATCA
FspI          716  TGCGCA               TGC^GCA
HincII        790  GTCAAC               GT[CT]^[GA]AC
HindII        790  GTCAAC               GT[CT]^[GA]AC
KspI         1063  CCGCGG               CCGC^GG
MflI          950  GGATCC               [GA]^GATC[CT]
MseI          932  TTAA                 T^TAA
MslI         1175  CATGCCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI         1064  CGCG                 CG^CG
NcoI         1191  CCATGG               C^CATGG
NspI          429  GCATGT               [GA]CATG^[CT]
PflFI        1606  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        1039  CACGTG               CAC^GTG
PmlI         1039  CACGTG               CAC^GTG
PstI         1128  CTGCAG               CTGCA^G
PvuII        1246  CAGCTG               CAG^CTG
SacII        1063  CCGCGG               CCGC^GG
ScaI          971  AGTACT               AGT^ACT
SmaI          353  CCCGGG               CCC^GGG
StuI          830  AGGCCT               AGG^CCT
TaqI          779  TCGA                 T^CGA
TfiI         1536  GAATC                G^A[AT]TC
Tru9I         932  TTAA                 T^TAA
Tsp509I      1553  AATT                 ^AATT
Tth111I      1606  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       779  TCGA                 T^CGA
XcmI          338  CCACTGAAGCTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         950  GGATCC               [GA]^GATC[CT]
XmaCI         353  CCCGGG               C^CCGGG
XmaI          353  CCCGGG               C^CCGGG
XmnI         1317  GAAGCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AocI          636  CCTCAGG              CC^T[ACGT]AGG
             1431  CCTGAGG             
AspHI        1350  GAGCAC               G[AT]GC[AT]^C
             1408  GTGCTC              
BsiHKAI      1350  GAGCAC               G[AT]GC[AT]^C
             1408  GTGCTC              
BsrFI         442  GCCGGC               [GA]^CCGG[CT]
             1133  GCCGGC              
BstEII       1203  GGTCACC              G^GT[ACGT]ACC
             1254  GGTGACC             
BstPI        1203  GGTCACC              G^GT[ACGT]ACC
             1254  GGTGACC             
Bsu36I        636  CCTCAGG              CC^T[ACGT]AGG
             1431  CCTGAGG             
Cfr10I        442  GCCGGC               [GA]^CCGG[CT]
             1133  GCCGGC              
EarI         1321  CTCTTC               CTCTTC(1/4)
             1158  GAAGAG              
Eco81I        636  CCTCAGG              CC^T[ACGT]AGG
             1431  CCTGAGG             
EcoNI         237  CCTACAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              632  CCTACCTCAGG         
EcoO65I      1203  GGTCACC              G^GT[ACGT]ACC
             1254  GGTGACC             
Ksp632I      1321  CTCTTC               CTCTTC(1/4)
             1158  GAAGAG              
NaeI          442  GCCGGC               GCC^GGC
             1133  GCCGGC              
NgoMIV        442  GCCGGC               G^CCGGC
             1133  GCCGGC              
PpuMI         593  GGGACCT              [GA]G^G[AT]CC[CT]
             1058  AGGACCC             
SapI         1158  GAAGAGC              GCTCTTC(1/4)
             1320  GCTCTTC             


#########################################
# 3 OR MORE CUT ENZYMES (   77 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
ApaI        AvaI        AvaII       BalI        BanI        
BanII       BbvI        BcnI        BmyI        BpmI        
BsaJI       BseRI       BsgI        BsiYI       BslI        
BsmFI       BsoBI       Bsp1286I    BspMI       BsrI        
BssKI       BstF5I      BstNI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MaeII       MaeIII      MboI        MboII       
MluNI       MnlI        MscI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PleI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TseI        
Tsp45I      TspRI       
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