Restriction map for pF1KE1978
 (Linear) (Six-base) MAPSORT of: KIEE1978.seq  Check: 7550  from: 1  to: 1665



KIEE1978.orf

 With 182 enzymes: SgfI * 

                        June 30, 2019 14:46  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     223     730    1606    1665 
   Size:        223     507     876      59

  Fragments arranged by size: 

                 876     507     223      59
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     362     489    1665 
   Size:        362     127    1176

  Fragments arranged by size: 

                1176     362     127
 
AlwNI CAG_nnn'CTG

Cuts at:      0     261    1101    1341    1665 
   Size:        261     840     240     324

  Fragments arranged by size: 

                 840     324     261     240
 
ApaI G_GGCC'C

Cuts at:      0     291     588     965    1089    1430    1665 
   Size:        291     297     377     124     341     235

  Fragments arranged by size: 

                 377     341     297     291     235     124
 
ApoI r'AATT_y

Cuts at:      0    1552    1665 
   Size:       1552     113
 
AvaI C'yCGr_G

Cuts at:      0     165     353     589     964    1166    1665 
   Size:        165     188     236     375     202     499

  Fragments arranged by size: 

                 499     375     236     202     188     165
 
BamHI G'GATC_C

Cuts at:      0     950    1665 
   Size:        950     715
 
BanI G'GyrC_C

Cuts at:      0     211     247     658    1483    1594    1665 
   Size:        211      36     411     825     111      71

  Fragments arranged by size: 

                 825     411     211     111      71      36
 
BanII G_rGCy'C

Cuts at:      0     172     291     298     588     746     965    1089    1116 
   Size:        172     119       7     290     158     219     124      27

Cuts at:   1116    1165    1430    1665 
   Size:         49     265     235

  Fragments arranged by size: 

                 290     265     235     219     172     158     124     119
                  49      27       7
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     487    1665 
   Size:        487    1178
 
BbvCI CC'TCA_GC

Cuts at:      0    1342    1665 
   Size:       1342     323
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1157    1191    1665 
   Size:       1157      34     474

  Fragments arranged by size: 

                1157     474      34
 
BclI T'GATC_A

Cuts at:      0    1491    1665 
   Size:       1491     174
 
BglI GCCn_nnn'nGGC

Cuts at:      0     167    1665 
   Size:        167    1498
 
BlpI GC'TnA_GC

Cuts at:      0    1197    1665 
   Size:       1197     468
 
Bme1580I G_kGCm'C

Cuts at:      0     250     291     588     965    1089    1144    1430    1665 
   Size:        250      41     297     377     124      55     286     235

  Fragments arranged by size: 

                 377     297     286     250     235     124      55      41
            
 
BmgBI CAC'GTC

Cuts at:      0     147     402    1665 
   Size:        147     255    1263

  Fragments arranged by size: 

                1263     255     147
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     172    1665 
   Size:        172    1493
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     990    1665 
   Size:        990     675
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     238    1140    1270    1622    1665 
   Size:        238     902     130     352      43

  Fragments arranged by size: 

                 902     352     238     130      43
 
Bpu10I CC'TnA_GC

Cuts at:      0     410    1342    1665 
   Size:        410     932     323

  Fragments arranged by size: 

                 932     410     323
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1163    1665 
   Size:       1163     502
 
BsaAI yAC'GTr

Cuts at:      0    1041    1665 
   Size:       1041     624
 
BsaWI w'CCGG_w

Cuts at:      0    1646    1665 
   Size:       1646      19
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0       7     853    1095    1604    1665 
   Size:          7     846     242     509      61

  Fragments arranged by size: 

                 846     509     242      61       7
 
BseYI C'CCAG_C

Cuts at:      0     623     684    1244    1665 
   Size:        623      61     560     421

  Fragments arranged by size: 

                 623     560     421      61
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     201     550    1021    1665 
   Size:        201     349     471     644

  Fragments arranged by size: 

                 644     471     349     201
 
BsiEI CG_ry'CG

Cuts at:      0    1138    1665 
   Size:       1138     527
 
BsiHKAI G_wGCw'C

Cuts at:      0    1354    1412    1665 
   Size:       1354      58     253

  Fragments arranged by size: 

                1354     253      58
 
Bsp1286I G_dGCh'C

Cuts at:      0     172     250     291     298     588     746     965    1089 
   Size:        172      78      41       7     290     158     219     124

Cuts at:   1089    1116    1144    1165    1354    1412    1430    1665 
   Size:         27      28      21     189      58      18     235

  Fragments arranged by size: 

                 290     235     219     189     172     158     124      78
                  58      41      28      27      21      18       7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     223     605     730     947    1431    1606    1665 
   Size:        223     382     125     217     484     175      59

  Fragments arranged by size: 

                 484     382     223     217     175     125      59
 
BsrBI CCG'CTC

Cuts at:      0     164    1665 
   Size:        164    1501
 
BsrDI GCAATG_nn'

Cuts at:      0     429    1665 
   Size:        429    1236
 
BsrFI r'CCGG_y

Cuts at:      0     442    1133    1665 
   Size:        442     691     532

  Fragments arranged by size: 

                 691     532     442
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1044    1665 
   Size:       1044     621
 
BstEII G'GTnAC_C

Cuts at:      0    1203    1254    1665 
   Size:       1203      51     411

  Fragments arranged by size: 

                1203     411      51
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     298    1665 
   Size:        298    1367
 
BstYI r'GATC_y

Cuts at:      0     950    1665 
   Size:        950     715
 
Bsu36I CC'TnA_GG

Cuts at:      0     637    1432    1665 
   Size:        637     795     233

  Fragments arranged by size: 

                 795     637     233
 
BtgI C'CryG_G

Cuts at:      0     804    1063    1191    1301    1665 
   Size:        804     259     128     110     364

  Fragments arranged by size: 

                 804     364     259     128     110
 
BtsI GCAGTG_nn'

Cuts at:      0     707    1295    1665 
   Size:        707     588     370

  Fragments arranged by size: 

                 707     588     370
 
DraIII CAC_nnn'GTG

Cuts at:      0    1358    1665 
   Size:       1358     307
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     388    1665 
   Size:        388    1277
 
EaeI y'GGCC_r

Cuts at:      0     128     440     690     915    1135    1332    1415    1542 
   Size:        128     312     250     225     220     197      83     127

Cuts at:   1542    1665 
   Size:        123

  Fragments arranged by size: 

                 312     250     225     220     197     128     127     123
                  83
 
EagI C'GGCC_G

Cuts at:      0    1135    1665 
   Size:       1135     530
 
EarI CTCTTCn'nnn_

Cuts at:      0    1153    1327    1665 
   Size:       1153     174     338

  Fragments arranged by size: 

                1153     338     174
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     148    1665 
   Size:        148    1517
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     238     362     489    1140    1270    1622    1665 
   Size:        238     124     127     651     130     352      43

  Fragments arranged by size: 

                 651     352     238     130     127     124      43
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     241     636    1665 
   Size:        241     395    1029

  Fragments arranged by size: 

                1029     395     241
 
EcoO109I rG'GnC_Cy

Cuts at:      0     584     585     594    1059    1085    1086    1236    1275 
   Size:        584       1       9     465      26       1     150      39

Cuts at:   1275    1426    1427    1665 
   Size:        151       1     238

  Fragments arranged by size: 

                 584     465     238     151     150      39      26       9
                   1       1       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1341    1665 
   Size:       1341     324
 
FspI TGC'GCA

Cuts at:      0     718    1665 
   Size:        718     947
 
HaeII r_GCGC'y

Cuts at:      0     261     274     312     897    1665 
   Size:        261      13      38     585     768

  Fragments arranged by size: 

                 768     585     261      38      13
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1441    1473    1665 
   Size:       1441      32     192

  Fragments arranged by size: 

                1441     192      32
 
HincII GTy'rAC

Cuts at:      0     792    1665 
   Size:        792     873
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     674    1665 
   Size:        674     991
 
MscI TGG'CCA

Cuts at:      0    1334    1417    1544    1665 
   Size:       1334      83     127     121

  Fragments arranged by size: 

                1334     127     121      83
 
MslI CAynn'nnrTG

Cuts at:      0    1179    1665 
   Size:       1179     486
 
MspA1I CmG'CkG

Cuts at:      0     564    1065    1201    1248    1665 
   Size:        564     501     136      47     417

  Fragments arranged by size: 

                 564     501     417     136      47
 
NaeI GCC'GGC

Cuts at:      0     444    1135    1665 
   Size:        444     691     530

  Fragments arranged by size: 

                 691     530     444
 
NcoI C'CATG_G

Cuts at:      0    1191    1665 
   Size:       1191     474
 
NgoMIV G'CCGG_C

Cuts at:      0     442    1133    1665 
   Size:        442     691     532

  Fragments arranged by size: 

                 691     532     442
 
NspI r_CATG'y

Cuts at:      0     433    1665 
   Size:        433    1232
 
PasI CC'CwG_GG

Cuts at:      0     291    1665 
   Size:        291    1374
 
PfoI T'CCnGG_A

Cuts at:      0    1638    1665 
   Size:       1638      27
 
PmlI CAC'GTG

Cuts at:      0    1041    1665 
   Size:       1041     624
 
PpuMI rG'GwC_Cy

Cuts at:      0     594    1059    1665 
   Size:        594     465     606

  Fragments arranged by size: 

                 606     594     465
 
PspOMI G'GGCC_C

Cuts at:      0     287     584     961    1085    1426    1665 
   Size:        287     297     377     124     341     239

  Fragments arranged by size: 

                 377     341     297     287     239     124
 
PstI C_TGCA'G

Cuts at:      0    1132    1665 
   Size:       1132     533
 
PvuII CAG'CTG

Cuts at:      0    1248    1665 
   Size:       1248     417
 
SacII CC_GC'GG

Cuts at:      0    1066    1665 
   Size:       1066     599
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1153    1327    1665 
   Size:       1153     174     338

  Fragments arranged by size: 

                1153     338     174
 
ScaI AGT'ACT

Cuts at:      0     973    1665 
   Size:        973     692
 
SfcI C'TryA_G

Cuts at:      0     238     534    1128    1665 
   Size:        238     296     594     537

  Fragments arranged by size: 

                 594     537     296     238
 
SmaI CCC'GGG

Cuts at:      0     355    1665 
   Size:        355    1310
 
StuI AGG'CCT

Cuts at:      0     832    1665 
   Size:        832     833
 
StyI C'CwwG_G

Cuts at:      0     827    1191    1479    1665 
   Size:        827     364     288     186

  Fragments arranged by size: 

                 827     364     288     186
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     637     676    1225    1665 
   Size:        637      39     549     440

  Fragments arranged by size: 

                 637     549     440      39
 
TatI w'GTAC_w

Cuts at:      0     497     971    1665 
   Size:        497     474     694

  Fragments arranged by size: 

                 694     497     474
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     179     211    1665 
   Size:        179      32    1454

  Fragments arranged by size: 

                1454     179      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1609    1665 
   Size:       1609      56
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     345    1665 
   Size:        345    1320
 
XmaI C'CCGG_G

Cuts at:      0     353    1665 
   Size:        353    1312
 
XmnI GAAnn'nnTTC

Cuts at:      0    1321    1665 
   Size:       1321     344
 

 Enzymes that do cut:

     AarI     AcuI    AlwNI     ApaI     ApoI     AvaI    BamHI     BanI
    BanII     BbsI    BbvCI     BcgI     BclI     BglI     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaWI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspMI    BsrBI
    BsrDI    BsrFI   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI
     BtsI   DraIII     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
    EcoNI EcoO109I     FalI     FspI    HaeII    Hin4I   HincII     MmeI
     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV     NspI     PasI
     PfoI     PmlI    PpuMI   PspOMI     PstI    PvuII    SacII     SapI
     ScaI     SfcI     SmaI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI     BbeI    BciVI    BfrBI    BglII     BmtI    BpuEI
    BsaBI    BsaHI    BsaXI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrGI   BssHII    BssSI    BstBI  BstZ17I    BtgZI     ClaI    CspCI
     DraI  EcoICRI    EcoRI    EcoRV     FseI    FspAI  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     NarI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PciI    PflMI     PmeI     PpiI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII     SacI     SalI    SanDI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI     XbaI     XhoI     ZraI
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