Restriction map for pF1KE0420
##################################
# NO CUT ENZYMES (  144 enzymes) #
##################################

AatII       AccI        AclI        AcsI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BsaBI       BsaI        BsiEI       
BsiHKAI     BsiWI       BsmAI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1407I    BspDI       BspLU11I    
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      Bst1107I    BstAPI      BstBI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HgaI        HincII      HindII      
HindIII     HpaI        KspI        MamI        MfeI        
MluI        MluNI       MscI        MseI        MunI        
NaeI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmeI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tru9I       Tth111I     Van91I      
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   60 enzymes) #
###################################

Acc65I         77  GGTACC               G^GTACC
AccII         375  CGCG                 CG^CG
AccIII        455  TCCGGA               T^CCGGA
AcyI          235  GGCGCC               G[GA]^CG[CT]C
Asp700I       270  GAACCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I        77  GGTACC               G^GTACC
BbeI          235  GGCGCC               GGCGC^C
BbrPI         574  CACGTG               CAC^GTG
BbsI          292  GAAGAC               GAAGAC(2/6)
BglII         285  AGATCT               A^GATCT
BpuAI         292  GAAGAC               GAAGAC(2/6)
BsaAI         574  CACGTG               [CT]AC^GT[GA]
BsaHI         235  GGCGCC               G[GA]^CG[CT]C
BsaWI         455  TCCGGA               [AT]^CCGG[AT]
BseAI         455  TCCGGA               T^CCGGA
BseRI         100  GAGGAG               GAGGAG(10/8)
BsgI          162  GTGCAG               GTGCAG(16/14)
BspEI         455  TCCGGA               T^CCGGA
BspHI         320  TCATGA               T^CATGA
BspMI         483  GCAGGT               ACCTGC(4/8)
BssSI          82  CACGAG               CACGAG(-5/-1)
BstEII        513  GGTGACC              G^GT[ACGT]ACC
BstPI         513  GGTGACC              G^GT[ACGT]ACC
BstUI         375  CGCG                 CG^CG
BstXI         569  CCAGCCACGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         285  AGATCT               [GA]^GATC[CT]
CfoI          236  GCGC                 GCG^C
DraII         350  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoNI         138  CCTGGAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      350  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       513  GGTGACC              G^GT[ACGT]ACC
FauI          373  CCCGC                CCCGC(4/6)
HaeII         235  GGCGCC               [GA]GCGC^[CT]
HapII         456  CCGG                 C^CGG
HhaI          236  GCGC                 GCG^C
Hin1I         235  GGCGCC               G[GA]^CG[CT]C
HinP1I        236  GCGC                 G^CGC
HpaII         456  CCGG                 C^CGG
KasI          235  GGCGCC               G^GCGCC
KpnI           77  GGTACC               GGTAC^C
MaeII         575  ACGT                 A^CGT
MflI          285  AGATCT               [GA]^GATC[CT]
MroI          455  TCCGGA               T^CCGGA
MslI          453  CATCCGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          456  CCGG                 C^CGG
MvnI          375  CGCG                 CG^CG
NarI          235  GGCGCC               GG^CGCC
NspI           66  GCATGT               [GA]CATG^[CT]
PleI          538  GAGTC                GAGTC(4/5)
PmaCI         574  CACGTG               CAC^GTG
PmlI          574  CACGTG               CAC^GTG
RcaI          320  TCATGA               T^CATGA
SfoI          235  GGCGCC               GGC^GCC
TaqI          314  TCGA                 T^CGA
TfiI            9  GAATC                G^A[AT]TC
Tsp509I       429  AATT                 ^AATT
TthHB8I       314  TCGA                 T^CGA
XbaI          288  TCTAGA               T^CTAGA
XhoII         285  AGATCT               [GA]^GATC[CT]
XmnI          270  GAACCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AciI          130  CCGC                 CCGC(-3/-1)
              374  CCGC                
AvaII          42  GGTCC                G^G[AT]CC
              445  GGACC               
BanI           77  GGTACC               G^G[CT][GA]CC
              235  GGCGCC              
BanII         331  GAGCCC               G[GA]GC[CT]^C
              610  GAGCCC              
BfaI          289  CTAG                 C^TAG
              403  CTAG                
BpmI          139  CTGGAG               CTGGAG(16/14)
              559  CTGGAG              
Cac8I         167  GCTGGC               GC[ACGT]^[ACGT]GC
              222  GCAGGC              
DdeI          362  CTGAG                C^T[ACGT]AG
              520  CTCAG               
DpnII         286  GATC                 ^GATC
              312  GATC                
EcoT14I       302  CCTTGG               C^C[AT][AT]GG
              354  CCTTGG              
HinfI           9  GAATC                G^A[ACGT]TC
              538  GAGTC               
HphI          513  GGTGA                GGTGA(8/7)
              299  TCACC               
MaeI          289  CTAG                 C^TAG
              403  CTAG                
MaeIII        394  GTGAC                ^GT[ACGT]AC
              514  GTGAC               
MboI          286  GATC                 ^GATC
              312  GATC                
NdeII         286  GATC                 ^GATC
              312  GATC                
PstI          106  CTGCAG               CTGCA^G
              339  CTGCAG              
PvuII         165  CAGCTG               CAG^CTG
              336  CAGCTG              
Sau3AI        286  GATC                 ^GATC
              312  GATC                
SfcI          106  CTGCAG               C^T[GA][CT]AG
              339  CTGCAG              
StyI          302  CCTTGG               C^C[AT][AT]GG
              354  CCTTGG              
Tsp45I        394  GTGAC                ^GT[GC]AC
              514  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   32 enzymes) #
#########################################

AfaI        AluI        BbvI        BmyI        BsaJI       
BsiYI       BslI        Bsp1286I    BssKI       BstF5I      
BstNI       Cfr13I      EarI        EcoRII      Fnu4HI      
FokI        HaeIII      ItaI        Ksp632I     MboII       
MnlI        MspA1I      MvaI        MwoI        NlaIII      
NlaIV       RsaI        Sau96I      ScrFI       SfaNI       
TseI        TspRI       
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