Restriction map for pF1KE0420
 (Linear) (Six-base) MAPSORT of: KIEE0420.seq  Check: 1410  from: 1  to: 618



KIEE0420.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     474     618 
   Size:        474     144
 
Acc65I G'GTAC_C

Cuts at:      0      77     618 
   Size:         77     541
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     167     194     618 
   Size:        167      27     424

  Fragments arranged by size: 

                 424     167      27
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     174     207     618 
   Size:        174      33     411

  Fragments arranged by size: 

                 411     174      33
 
BanI G'GyrC_C

Cuts at:      0      77     235     618 
   Size:         77     158     383

  Fragments arranged by size: 

                 383     158      77
 
BanII G_rGCy'C

Cuts at:      0     335     614     618 
   Size:        335     279       4

  Fragments arranged by size: 

                 335     279       4
 
BbeI G_GCGC'C

Cuts at:      0     239     618 
   Size:        239     379
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     299     618 
   Size:        299     319
 
BglII A'GATC_T

Cuts at:      0     285     618 
   Size:        285     333
 
Bme1580I G_kGCm'C

Cuts at:      0      74     618 
   Size:         74     544
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     160     580     618 
   Size:        160     420      38

  Fragments arranged by size: 

                 420     160      38
 
BsaAI yAC'GTr

Cuts at:      0     576     618 
   Size:        576      42
 
BsaHI Gr'CG_yC

Cuts at:      0     236     618 
   Size:        236     382
 
BsaWI w'CCGG_w

Cuts at:      0     455     618 
   Size:        455     163
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     133     163     618 
   Size:        133      30     455

  Fragments arranged by size: 

                 455     133      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     115     618 
   Size:        115     503
 
BseYI C'CCAG_C

Cuts at:      0      27     227     334     618 
   Size:         27     200     107     284

  Fragments arranged by size: 

                 284     200     107      27
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     183     618 
   Size:        183     435
 
Bsp1286I G_dGCh'C

Cuts at:      0      74     335     614     618 
   Size:         74     261     279       4

  Fragments arranged by size: 

                 279     261      74       4
 
BspEI T'CCGG_A

Cuts at:      0     455     618 
   Size:        455     163
 
BspHI T'CATG_A

Cuts at:      0     320     618 
   Size:        320     298
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     474     618 
   Size:        474     144
 
BssSI C'ACGA_G

Cuts at:      0      82     618 
   Size:         82     536
 
BstEII G'GTnAC_C

Cuts at:      0     513     618 
   Size:        513     105
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     576     618 
   Size:        576      42
 
BstYI r'GATC_y

Cuts at:      0     285     618 
   Size:        285     333
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     391     618 
   Size:        391     227
 
BtsI GCAGTG_nn'

Cuts at:      0     166     504     618 
   Size:        166     338     114

  Fragments arranged by size: 

                 338     166     114
 
EarI CTCTTCn'nnn_

Cuts at:      0     239     280     575     618 
   Size:        239      41     295      43

  Fragments arranged by size: 

                 295     239      43      41
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     160     580     618 
   Size:        160     420      38

  Fragments arranged by size: 

                 420     160      38
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     142     618 
   Size:        142     476
 
EcoO109I rG'GnC_Cy

Cuts at:      0     351     618 
   Size:        351     267
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     210     618 
   Size:        210     408
 
HaeII r_GCGC'y

Cuts at:      0     239     618 
   Size:        239     379
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      26     437     469     618 
   Size:         26     411      32     149

  Fragments arranged by size: 

                 411     149      32      26
 
KasI G'GCGC_C

Cuts at:      0     235     618 
   Size:        235     383
 
KpnI G_GTAC'C

Cuts at:      0      81     618 
   Size:         81     537
 
MslI CAynn'nnrTG

Cuts at:      0     457     618 
   Size:        457     161
 
MspA1I CmG'CkG

Cuts at:      0     132     167     338     618 
   Size:        132      35     171     280

  Fragments arranged by size: 

                 280     171     132      35
 
NarI GG'CG_CC

Cuts at:      0     236     618 
   Size:        236     382
 
NspI r_CATG'y

Cuts at:      0      70     618 
   Size:         70     548
 
PmlI CAC'GTG

Cuts at:      0     576     618 
   Size:        576      42
 
PstI C_TGCA'G

Cuts at:      0     110     343     618 
   Size:        110     233     275

  Fragments arranged by size: 

                 275     233     110
 
PvuII CAG'CTG

Cuts at:      0     167     338     618 
   Size:        167     171     280

  Fragments arranged by size: 

                 280     171     167
 
SfcI C'TryA_G

Cuts at:      0     106     339     618 
   Size:        106     233     279

  Fragments arranged by size: 

                 279     233     106
 
SfoI GGC'GCC

Cuts at:      0     237     618 
   Size:        237     381
 
StyI C'CwwG_G

Cuts at:      0     302     354     618 
   Size:        302      52     264

  Fragments arranged by size: 

                 302     264      52
 
XbaI T'CTAG_A

Cuts at:      0     288     618 
   Size:        288     330
 
XmnI GAAnn'nnTTC

Cuts at:      0     274     618 
   Size:        274     344
 

 Enzymes that do cut:

     AarI   Acc65I     AlfI     BaeI     BanI    BanII     BbeI     BbsI
    BglII Bme1580I     BpmI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI Bsp1286I    BspEI    BspHI    BspMI    BssSI   BstEII
    BstXI    BstYI    BtgZI     BtsI     EarI  Eco57MI    EcoNI EcoO109I
     FalI    HaeII    Hin4I     KasI     KpnI     MslI   MspA1I     NarI
     NspI     PmlI     PstI    PvuII     SfcI     SfoI     StyI     XbaI
     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII    BamHI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI     BlpI    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaBI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI
  BstZ17I   Bsu36I     BtgI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoRI    EcoRV     FseI     FspI
    FspAI   HincII  HindIII     HpaI     MfeI     MluI     MmeI     MscI
     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XcmI
     XhoI     XmaI     ZraI
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