Restriction map for
pF1KE0417
(Linear) (Six-base) MAPSORT of: KIEE0417.seq Check: 5032 from: 1 to: 762
KIEE0417.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 355 762
Size: 134 221 407
Fragments arranged by size:
407 221 134
AhdI GACnn_n'nnGTC
Cuts at: 0 672 762
Size: 672 90
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 424 456 762
Size: 424 32 306
Fragments arranged by size:
424 306 32
AlwNI CAG_nnn'CTG
Cuts at: 0 466 762
Size: 466 296
ApoI r'AATT_y
Cuts at: 0 220 368 762
Size: 220 148 394
Fragments arranged by size:
394 220 148
AvaI C'yCGr_G
Cuts at: 0 751 762
Size: 751 11
BanI G'GyrC_C
Cuts at: 0 646 694 762
Size: 646 48 68
Fragments arranged by size:
646 68 48
BanII G_rGCy'C
Cuts at: 0 484 551 762
Size: 484 67 211
Fragments arranged by size:
484 211 67
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 549 583 762
Size: 549 34 179
Fragments arranged by size:
549 179 34
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 696 762
Size: 696 66
Bpu10I CC'TnA_GC
Cuts at: 0 524 762
Size: 524 238
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 359 762
Size: 340 19 403
Fragments arranged by size:
403 340 19
BsaI GGTCTCn'nnnn_
Cuts at: 0 250 762
Size: 250 512
BsaWI w'CCGG_w
Cuts at: 0 516 709 762
Size: 516 193 53
Fragments arranged by size:
516 193 53
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 424 454 762
Size: 424 30 308
Fragments arranged by size:
424 308 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 151 714 762
Size: 151 563 48
Fragments arranged by size:
563 151 48
BsiHKAI G_wGCw'C
Cuts at: 0 484 762
Size: 484 278
Bsp1286I G_dGCh'C
Cuts at: 0 484 551 762
Size: 484 67 211
Fragments arranged by size:
484 211 67
BstBI TT'CG_AA
Cuts at: 0 105 762
Size: 105 657
BstEII G'GTnAC_C
Cuts at: 0 19 762
Size: 19 743
BstYI r'GATC_y
Cuts at: 0 117 762
Size: 117 645
BtsI GCAGTG_nn'
Cuts at: 0 600 762
Size: 600 162
EarI CTCTTCn'nnn_
Cuts at: 0 306 762
Size: 306 456
EcoICRI GAG'CTC
Cuts at: 0 482 762
Size: 482 280
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 355 762
Size: 134 221 407
Fragments arranged by size:
407 221 134
EcoNI CCTnn'n_nnAGG
Cuts at: 0 271 358 762
Size: 271 87 404
Fragments arranged by size:
404 271 87
EcoO109I rG'GnC_Cy
Cuts at: 0 756 762
Size: 756 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 139 171 538 570 658 690 762
Size: 139 32 367 32 88 32 72
Fragments arranged by size:
367 139 88 72 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 345 762
Size: 345 417
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 199 231 424 456 762
Size: 199 32 193 32 306
Fragments arranged by size:
306 199 193 32 32
PspXI vC'TCGA_Gb
Cuts at: 0 751 762
Size: 751 11
PstI C_TGCA'G
Cuts at: 0 618 762
Size: 618 144
SacI G_AGCT'C
Cuts at: 0 484 762
Size: 484 278
SfcI C'TryA_G
Cuts at: 0 172 419 614 762
Size: 172 247 195 148
Fragments arranged by size:
247 195 172 148
SmlI C'TyrA_G
Cuts at: 0 355 374 751 762
Size: 355 19 377 11
Fragments arranged by size:
377 355 19 11
StuI AGG'CCT
Cuts at: 0 641 762
Size: 641 121
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 634 762
Size: 634 128
XhoI C'TCGA_G
Cuts at: 0 751 762
Size: 751 11
XmnI GAAnn'nnTTC
Cuts at: 0 436 762
Size: 436 326
Enzymes that do cut:
AcuI AhdI AloI AlwNI ApoI AvaI BanI BanII
BcgI BplI Bpu10I BpuEI BsaI BsaWI BsaXI BseRI
BsiHKAI Bsp1286I BstBI BstEII BstYI BtsI EarI EcoICRI
Eco57MI EcoNI EcoO109I Hin4I MmeI PpiI PspXI PstI
SacI SfcI SmlI StuI TaqII XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BsaAI BsaBI BsaHI BseYI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstXI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI
EciI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI PsrI
PvuI PvuII RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StyI SwaI TatI TstI
Tth111I XbaI XcmI XmaI ZraI