(Linear) (Six-base) MAPSORT of: KIEE0415.seq  Check: 5536  from: 1  to: 471



KIEE0415.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AccI GT'mk_AC

Cuts at:      0     380     471 
   Size:        380      91
 
AseI AT'TA_AT

Cuts at:      0     209     471 
   Size:        209     262
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     464     471 
   Size:        464       7
 
BfrBI ATG'CAT

Cuts at:      0      38     471 
   Size:         38     433
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      54     471 
   Size:         54     417
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      42     471 
   Size:         42     429
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     341     371     471 
   Size:        341      30     100

  Fragments arranged by size: 

                 341     100      30
 
BsiHKAI G_wGCw'C

Cuts at:      0     279     471 
   Size:        279     192
 
BsmI GAATG_Cn'

Cuts at:      0      40     414     471 
   Size:         40     374      57

  Fragments arranged by size: 

                 374      57      40
 
Bsp1286I G_dGCh'C

Cuts at:      0     279     471 
   Size:        279     192
 
BsrDI GCAATG_nn'

Cuts at:      0      74     471 
   Size:         74     397
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     175     471 
   Size:        175     296
 
BstYI r'GATC_y

Cuts at:      0     316     471 
   Size:        316     155
 
BstZ17I GTA'TAC

Cuts at:      0     381     471 
   Size:        381      90
 
EaeI y'GGCC_r

Cuts at:      0     447     471 
   Size:        447      24
 
MslI CAynn'nnrTG

Cuts at:      0     173     333     471 
   Size:        173     160     138

  Fragments arranged by size: 

                 173     160     138
 
MspA1I CmG'CkG

Cuts at:      0     127     471 
   Size:        127     344
 
NsiI A_TGCA'T

Cuts at:      0      40     471 
   Size:         40     431
 
PvuII CAG'CTG

Cuts at:      0     127     471 
   Size:        127     344
 
SmlI C'TyrA_G

Cuts at:      0      21     471 
   Size:         21     450
 
TatI w'GTAC_w

Cuts at:      0      87     471 
   Size:         87     384
 
XbaI T'CTAG_A

Cuts at:      0      12     471 
   Size:         12     459
 
XmnI GAAnn'nnTTC

Cuts at:      0     151     412     471 
   Size:        151     261      59

  Fragments arranged by size: 

                 261     151      59
 

 Enzymes that do cut:

     AccI     AseI     BbsI    BfrBI     BmrI    BpuEI    BsaXI  BsiHKAI
     BsmI Bsp1286I    BsrDI    BstXI    BstYI  BstZ17I     EaeI     MslI
   MspA1I     NsiI    PvuII     SmlI     TatI     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI
    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TstI
  Tth111I     XcmI     XhoI     XmaI     ZraI