Restriction map for pF1KE0396
 (Linear) (Six-base) MAPSORT of: KIEE0396.seq  Check: 5391  from: 1  to: 3120



KIEE0396.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1460    3120 
   Size:       1460    1660
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     828    1267    1996    2780    2956    2983    3120 
   Size:        828     439     729     784     176      27     137

  Fragments arranged by size: 

                 828     784     729     439     176     137      27
 
AflIII A'CryG_T

Cuts at:      0     603    1688    3120 
   Size:        603    1085    1432

  Fragments arranged by size: 

                1432    1085     603
 
AhdI GACnn_n'nnGTC

Cuts at:      0     342    1274    3120 
   Size:        342     932    1846

  Fragments arranged by size: 

                1846     932     342
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     940    3120 
   Size:        940    2180
 
AlwNI CAG_nnn'CTG

Cuts at:      0     933    1215    1494    2430    2709    2754    3120 
   Size:        933     282     279     936     279      45     366

  Fragments arranged by size: 

                 936     933     366     282     279     279      45
 
ApaI G_GGCC'C

Cuts at:      0    2326    2748    3120 
   Size:       2326     422     372

  Fragments arranged by size: 

                2326     422     372
 
ApaLI G'TGCA_C

Cuts at:      0    2576    3120 
   Size:       2576     544
 
ApoI r'AATT_y

Cuts at:      0     970    1798    2943    3120 
   Size:        970     828    1145     177

  Fragments arranged by size: 

                1145     970     828     177
 
AvaI C'yCGr_G

Cuts at:      0     392    1387    2048    2209    3120 
   Size:        392     995     661     161     911

  Fragments arranged by size: 

                 995     911     661     392     161
 
AvrII C'CTAG_G

Cuts at:      0    2991    3120 
   Size:       2991     129
 
BanI G'GyrC_C

Cuts at:      0     257    1788    2105    2294    2827    3120 
   Size:        257    1531     317     189     533     293

  Fragments arranged by size: 

                1531     533     317     293     257     189
 
BanII G_rGCy'C

Cuts at:      0     817     842    1388    2326    2664    2684    2748    3120 
   Size:        817      25     546     938     338      20      64     372

  Fragments arranged by size: 

                 938     817     546     372     338      64      25      20
            
 
BbeI G_GCGC'C

Cuts at:      0     261    1792    2109    3120 
   Size:        261    1531     317    1011

  Fragments arranged by size: 

                1531    1011     317     261
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     975    1088    1452    1606    3120 
   Size:        975     113     364     154    1514

  Fragments arranged by size: 

                1514     975     364     154     113
 
BbvCI CC'TCA_GC

Cuts at:      0     676    1428    3120 
   Size:        676     752    1692

  Fragments arranged by size: 

                1692     752     676
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2477    2511    3120 
   Size:       2477      34     609

  Fragments arranged by size: 

                2477     609      34
 
BclI T'GATC_A

Cuts at:      0    2772    3120 
   Size:       2772     348
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2032    3120 
   Size:       2032    1088
 
BlpI GC'TnA_GC

Cuts at:      0    2591    2794    3120 
   Size:       2591     203     326

  Fragments arranged by size: 

                2591     326     203
 
Bme1580I G_kGCm'C

Cuts at:      0    2121    2297    2326    2580    2748    2832    3120 
   Size:       2121     176      29     254     168      84     288

  Fragments arranged by size: 

                2121     288     254     176     168      84      29
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1604    2067    3120 
   Size:       1604     463    1053

  Fragments arranged by size: 

                1604    1053     463
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     213    3120 
   Size:        213    2907
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     763     790     853    1693    2884    2905    3120 
   Size:        763      27      63     840    1191      21     215

  Fragments arranged by size: 

                1191     840     763     215      63      27      21
 
Bpu10I CC'TnA_GC

Cuts at:      0     676    1428    3120 
   Size:        676     752    1692

  Fragments arranged by size: 

                1692     752     676
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1315    2284    2805    3120 
   Size:       1315     969     521     315

  Fragments arranged by size: 

                1315     969     521     315
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     141     389    1417    3120 
   Size:        141     248    1028    1703

  Fragments arranged by size: 

                1703    1028     248     141
 
BsaAI yAC'GTr

Cuts at:      0    1689    2278    3120 
   Size:       1689     589     842

  Fragments arranged by size: 

                1689     842     589
 
BsaBI GATnn'nnATC

Cuts at:      0    1942    3120 
   Size:       1942    1178
 
BsaHI Gr'CG_yC

Cuts at:      0     258    1789    2106    3120 
   Size:        258    1531     317    1014

  Fragments arranged by size: 

                1531    1014     317     258
 
BsaWI w'CCGG_w

Cuts at:      0    2228    3120 
   Size:       2228     892
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1498    1528    2143    2173    2651    2681    3120 
   Size:       1498      30     615      30     478      30     439

  Fragments arranged by size: 

                1498     615     478     439      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      46      49     126     850    2266    2902    3091    3120 
   Size:         46       3      77     724    1416     636     189      29

  Fragments arranged by size: 

                1416     724     636     189      77      46      29       3
            
 
BseYI C'CCAG_C

Cuts at:      0     365     387     816    1700    2631    3120 
   Size:        365      22     429     884     931     489

  Fragments arranged by size: 

                 931     884     489     429     365      22
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     919    1420    1627    2159    2258    3120 
   Size:        919     501     207     532      99     862

  Fragments arranged by size: 

                 919     862     532     501     207      99
 
BsiEI CG_ry'CG

Cuts at:      0    1193    1283    3120 
   Size:       1193      90    1837

  Fragments arranged by size: 

                1837    1193      90
 
BsiHKAI G_wGCw'C

Cuts at:      0      88     842     919    1055    2057    2517    2580    3120 
   Size:         88     754      77     136    1002     460      63     540

  Fragments arranged by size: 

                1002     754     540     460     136      88      77      63
            
 
BsmI GAATG_Cn'

Cuts at:      0    1257    3120 
   Size:       1257    1863
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     724    3120 
   Size:        724    2396
 
Bsp1286I G_dGCh'C

Cuts at:      0      88     817     842     919    1055    1388    2057    2121 
   Size:         88     729      25      77     136     333     669      64

Cuts at:   2121    2297    2326    2517    2580    2664    2684    2748    2832 
   Size:        176      29     191      63      84      20      64      84

Cuts at:   2832    3120 
   Size:        288

  Fragments arranged by size: 

                 729     669     333     288     191     176     136      88
                  84      84      77      64      64      63      29      25
                  20
 
BspEI T'CCGG_A

Cuts at:      0    2228    3120 
   Size:       2228     892
 
BspHI T'CATG_A

Cuts at:      0    1380    3120 
   Size:       1380    1740
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     407    1250    1460    1550    3120 
   Size:        407     843     210      90    1570

  Fragments arranged by size: 

                1570     843     407     210      90
 
BsrBI CCG'CTC

Cuts at:      0     474    2256    3120 
   Size:        474    1782     864

  Fragments arranged by size: 

                1782     864     474
 
BsrFI r'CCGG_y

Cuts at:      0     410     998    3120 
   Size:        410     588    2122

  Fragments arranged by size: 

                2122     588     410
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     933    1563    1682    1852    2205    2430    3120 
   Size:        933     630     119     170     353     225     690

  Fragments arranged by size: 

                 933     690     630     353     225     170     119
 
BstEII G'GTnAC_C

Cuts at:      0    1266    3120 
   Size:       1266    1854
 
Bsu36I CC'TnA_GG

Cuts at:      0    1300    1366    3120 
   Size:       1300      66    1754

  Fragments arranged by size: 

                1754    1300      66
 
BtgI C'CryG_G

Cuts at:      0     563     683    2375    2585    2654    3120 
   Size:        563     120    1692     210      69     466

  Fragments arranged by size: 

                1692     563     466     210     120      69
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     294    3120 
   Size:        294    2826
 
BtsI GCAGTG_nn'

Cuts at:      0     912    1015    1053    1179    2322    2520    2648    2826 
   Size:        912     103      38     126    1143     198     128     178

Cuts at:   2826    3120 
   Size:        294

  Fragments arranged by size: 

                1143     912     294     198     178     128     126     103
                  38
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1853    1888    3120 
   Size:       1853      35    1232

  Fragments arranged by size: 

                1853    1232      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1852    1940    2182    2333    3120 
   Size:       1852      88     242     151     787

  Fragments arranged by size: 

                1852     787     242     151      88
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     687    3120 
   Size:        687    2433
 
EaeI y'GGCC_r

Cuts at:      0     251     412     668     855    1016    1280    1919    2672 
   Size:        251     161     256     187     161     264     639     753

Cuts at:   2672    3120 
   Size:        448

  Fragments arranged by size: 

                 753     639     448     264     256     251     187     161
                 161
 
EagI C'GGCC_G

Cuts at:      0    1280    3120 
   Size:       1280    1840
 
EarI CTCTTCn'nnn_

Cuts at:      0       2     805     847    1225    1891    2522    3120 
   Size:          2     803      42     378     666     631     598

  Fragments arranged by size: 

                 803     666     631     598     378      42       2
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1399    1411    3120 
   Size:       1399      12    1709

  Fragments arranged by size: 

                1709    1399      12
 
EcoICRI GAG'CTC

Cuts at:      0     840    3120 
   Size:        840    2280
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     763     790     828     853    1267    1693    1996    2780 
   Size:        763      27      38      25     414     426     303     784

Cuts at:   2780    2884    2905    2956    2983    3120 
   Size:        104      21      51      27     137

  Fragments arranged by size: 

                 784     763     426     414     303     137     104      51
                  38      27      27      25      21
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1231    3010    3120 
   Size:       1231    1779     110

  Fragments arranged by size: 

                1779    1231     110
 
EcoO109I rG'GnC_Cy

Cuts at:      0     143     192     369    1514    1606    1668    2061    2094 
   Size:        143      49     177    1145      92      62     393      33

Cuts at:   2094    2323    2744    2751    3015    3120 
   Size:        229     421       7     264     105

  Fragments arranged by size: 

                1145     421     393     264     229     177     143     105
                  92      62      49      33       7
 
EcoRI G'AATT_C

Cuts at:      0    1798    3120 
   Size:       1798    1322
 
HaeII r_GCGC'y

Cuts at:      0     261    1377    1792    2109    3120 
   Size:        261    1116     415     317    1011

  Fragments arranged by size: 

                1116    1011     415     317     261
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      35      67     328     360     361     393    1186    1218 
   Size:         35      32     261      32       1      32     793      32

Cuts at:   1218    1260    1292    1498    1530    2383    2415    3120 
   Size:         42      32     206      32     853      32     705

  Fragments arranged by size: 

                 853     793     705     261     206      42      35      32
                  32      32      32      32      32      32       1
 
HincII GTy'rAC

Cuts at:      0    1127    2306    3084    3120 
   Size:       1127    1179     778      36

  Fragments arranged by size: 

                1179    1127     778      36
 
HpaI GTT'AAC

Cuts at:      0    1127    3120 
   Size:       1127    1993
 
KasI G'GCGC_C

Cuts at:      0     257    1788    2105    3120 
   Size:        257    1531     317    1015

  Fragments arranged by size: 

                1531    1015     317     257
 
MfeI C'AATT_G

Cuts at:      0    1890    2530    3120 
   Size:       1890     640     590

  Fragments arranged by size: 

                1890     640     590
 
MscI TGG'CCA

Cuts at:      0     253     670     857    1018    1921    3120 
   Size:        253     417     187     161     903    1199

  Fragments arranged by size: 

                1199     903     417     253     187     161
 
MslI CAynn'nnrTG

Cuts at:      0     440     863    2416    3120 
   Size:        440     423    1553     704

  Fragments arranged by size: 

                1553     704     440     423
 
MspA1I CmG'CkG

Cuts at:      0     120     930     995    1005    1285    2123    2173    2388 
   Size:        120     810      65      10     280     838      50     215

Cuts at:   2388    2427    2656    3120 
   Size:         39     229     464

  Fragments arranged by size: 

                 838     810     464     280     229     215     120      65
                  50      39      10
 
NaeI GCC'GGC

Cuts at:      0    1000    3120 
   Size:       1000    2120
 
NarI GG'CG_CC

Cuts at:      0     258    1789    2106    3120 
   Size:        258    1531     317    1014

  Fragments arranged by size: 

                1531    1014     317     258
 
NcoI C'CATG_G

Cuts at:      0    2585    3120 
   Size:       2585     535
 
NgoMIV G'CCGG_C

Cuts at:      0     998    3120 
   Size:        998    2122
 
NspI r_CATG'y

Cuts at:      0     456     607     656    1573    1680    2203    2606    3120 
   Size:        456     151      49     917     107     523     403     514

  Fragments arranged by size: 

                 917     523     514     456     403     151     107      49
            
 
PasI CC'CwG_GG

Cuts at:      0     825    3120 
   Size:        825    2295
 
PciI A'CATG_T

Cuts at:      0     603    3120 
   Size:        603    2517
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     228    1602    2670    2754    2838    3120 
   Size:        228    1374    1068      84      84     282

  Fragments arranged by size: 

                1374    1068     282     228      84      84
 
PfoI T'CCnGG_A

Cuts at:      0     167     767     965    2900    3038    3120 
   Size:        167     600     198    1935     138      82

  Fragments arranged by size: 

                1935     600     198     167     138      82
 
PmlI CAC'GTG

Cuts at:      0    2278    3120 
   Size:       2278     842
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1138    1170    3052    3084    3120 
   Size:       1138      32    1882      32      36

  Fragments arranged by size: 

                1882    1138      36      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     143     192     369    1514    1606    3120 
   Size:        143      49     177    1145      92    1514

  Fragments arranged by size: 

                1514    1145     177     143      92      49
 
PspOMI G'GGCC_C

Cuts at:      0    2322    2744    3120 
   Size:       2322     422     376

  Fragments arranged by size: 

                2322     422     376
 
PstI C_TGCA'G

Cuts at:      0     404     929     954    1010    1235    1505    1556    1991 
   Size:        404     525      25      56     225     270      51     435

Cuts at:   1991    2360    2387    3014    3100    3120 
   Size:        369      27     627      86      20

  Fragments arranged by size: 

                 627     525     435     404     369     270     225      86
                  56      51      27      25      20
 
PvuII CAG'CTG

Cuts at:      0     120     995    1005    2173    2388    2427    3120 
   Size:        120     875      10    1168     215      39     693

  Fragments arranged by size: 

                1168     875     693     215     120      39      10
 
RsrII CG'GwC_CG

Cuts at:      0    1398    3120 
   Size:       1398    1722
 
SacI G_AGCT'C

Cuts at:      0     842    3120 
   Size:        842    2278
 
SacII CC_GC'GG

Cuts at:      0    2657    3120 
   Size:       2657     463
 
SanDI GG'GwC_CC

Cuts at:      0     143     369    1514    1606    3120 
   Size:        143     226    1145      92    1514

  Fragments arranged by size: 

                1514    1145     226     143      92
 
SapI GCTCTTCn'nnn_

Cuts at:      0     805     847    1225    2522    3120 
   Size:        805      42     378    1297     598

  Fragments arranged by size: 

                1297     805     598     378      42
 
SbfI CC_TGCA'GG

Cuts at:      0    3014    3120 
   Size:       3014     106
 
SfcI C'TryA_G

Cuts at:      0     400     925     950    1006    1231    1501    1529    1552 
   Size:        400     525      25      56     225     270      28      23

Cuts at:   1552    1987    2356    2383    3010    3096    3120 
   Size:        435     369      27     627      86      24

  Fragments arranged by size: 

                 627     525     435     400     369     270     225      86
                  56      28      27      25      24      23
 
SfoI GGC'GCC

Cuts at:      0     259    1790    2107    3120 
   Size:        259    1531     317    1013

  Fragments arranged by size: 

                1531    1013     317     259
 
SmaI CCC'GGG

Cuts at:      0    1389    2050    2211    3120 
   Size:       1389     661     161     909

  Fragments arranged by size: 

                1389     909     661     161
 
SmlI C'TyrA_G

Cuts at:      0    1330    2299    2784    3120 
   Size:       1330     969     485     336

  Fragments arranged by size: 

                1330     969     485     336
 
SphI G_CATG'C

Cuts at:      0    1573    2203    2606    3120 
   Size:       1573     630     403     514

  Fragments arranged by size: 

                1573     630     514     403
 
StuI AGG'CCT

Cuts at:      0    1951    2113    3120 
   Size:       1951     162    1007

  Fragments arranged by size: 

                1951    1007     162
 
StyI C'CwwG_G

Cuts at:      0    1596    2439    2585    2765    2837    2991    3120 
   Size:       1596     843     146     180      72     154     129

  Fragments arranged by size: 

                1596     843     180     154     146     129      72
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     401     883    1207    2395    2501    2722    2758    2815 
   Size:        401     482     324    1188     106     221      36      57

Cuts at:   2815    3120 
   Size:        305

  Fragments arranged by size: 

                1188     482     401     324     305     221     106      57
                  36
 
TatI w'GTAC_w

Cuts at:      0    1307    3120 
   Size:       1307    1813
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2143    2175    2651    2683    3120 
   Size:       2143      32     476      32     437

  Fragments arranged by size: 

                2143     476     437      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     271    1117    1610    3120 
   Size:        271     846     493    1510

  Fragments arranged by size: 

                1510     846     493     271
 
XbaI T'CTAG_A

Cuts at:      0    3072    3120 
   Size:       3072      48
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     261     828    3120 
   Size:        261     567    2292

  Fragments arranged by size: 

                2292     567     261
 
XmaI C'CCGG_G

Cuts at:      0    1387    2048    2209    3120 
   Size:       1387     661     161     911

  Fragments arranged by size: 

                1387     911     661     161
 
XmnI GAAnn'nnTTC

Cuts at:      0    1802    3064    3120 
   Size:       1802    1262      56

  Fragments arranged by size: 

                1802    1262      56
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AhdI     AlfI    AlwNI     ApaI    ApaLI
     ApoI     AvaI    AvrII     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI     BclI     BglI     BlpI Bme1580I     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrFI   BstAPI   BstEII   Bsu36I
     BtgI    BtgZI     BtsI    CspCI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    HaeII
    Hin4I   HincII     HpaI     KasI     MfeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NspI     PasI     PciI    PflMI
     PfoI     PmlI     PpiI    PpuMI   PspOMI     PstI    PvuII    RsrII
     SacI    SacII    SanDI     SapI     SbfI     SfcI     SfoI     SmaI
     SmlI     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AleI
     AloI     AscI     AseI    AsiSI     BaeI    BamHI    BciVI    BfrBI
    BglII    BmgBI     BmtI    BsiWI    BsrDI    BsrGI   BssHII    BssSI
    BstBI    BstXI    BstYI  BstZ17I     ClaI     DraI    EcoRV     FalI
     FseI     FspI    FspAI  HindIII     KpnI     MluI     MmeI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PmeI    PshAI     PsiI
    PspXI     PsrI     PvuI     SalI     ScaI    SexAI     SfiI     SgfI
    SgrAI    SnaBI     SpeI     SrfI     SspI     SwaI     XhoI     ZraI
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