Restriction map for pF1KE0395
##################################
# NO CUT ENZYMES (   91 enzymes) #
##################################

AatII       AccIII      AclI        AcyI        AflII       
AgeI        AocI        AscI        AviII       AvrII       
BbeI        BbrPI       BbvCI       BciVI       BfrI        
BglI        BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BseAI       BsiWI       BspDI       
BspEI       BspHI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FseI        
FspI        HgaI        Hin1I       HpaI        KasI        
KspI        MamI        MfeI        MluI        MroI        
MunI        NarI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SapI        SbfI        SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspI        SwaI        Van91I      XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

Acc65I        557  GGTACC               G^GTACC
AccI         1534  GTAGAC               GT^[AC][GT]AC
AccII        3026  CGCG                 CG^CG
AhdI         2671  GACAAAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       3544  GTGCAC               G^TGCAC
Aor51HI      1165  AGCGCT               AGC^GCT
ApaLI        3544  GTGCAC               G^TGCAC
AseI         1200  ATTAAT               AT^TAAT
AsnI         1200  ATTAAT               AT^TAAT
Asp700I       782  GAAGAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       557  GGTACC               G^GTACC
AspEI        2671  GACAAAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI         147  GGATCC               G^GATCC
BanI          557  GGTACC               G^G[CT][GA]CC
BcgI          723  CGATGAGTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII        2718  AGATCT               A^GATCT
BsaI         3117  GGTCTC               GGTCTC(1/5)
BsaWI        2993  ACCGGA               [AT]^CCGG[AT]
BsiEI        3028  CGGCCG               CG[GA][CT]^CG
BsmBI        1828  GAGACG               CGTCTC(1/5)
BsmI         3180  GCATTC               GAATGC(1/-1)
BspMI        2663  ACCTGC               ACCTGC(4/8)
BsrBI         561  CCGCTC               CCGCTC(-3/-3)
BstUI        3026  CGCG                 CG^CG
BstXI        2091  CCAAGTGAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI         1039  TTTAAA               TTT^AAA
DrdI         2392  GACTCCTACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         3028  CGGCCG               C^GGCCG
Eam1105I     2671  GACAAAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        3028  CGGCCG               C^GGCCG
Eco47III     1165  AGCGCT               AGC^GCT
Eco52I       3028  CGGCCG               C^GGCCG
HaeII        1165  AGCGCT               [GA]GCGC^[CT]
HincII        444  GTCAAC               GT[CT]^[GA]AC
HindII        444  GTCAAC               GT[CT]^[GA]AC
KpnI          557  GGTACC               GGTAC^C
MvnI         3026  CGCG                 CG^CG
PshBI        1200  ATTAAT               AT^TAAT
SacI          166  GAGCTC               GAGCT^C
SexAI         323  ACCTGGT              A^CC[AT]GGT
SmaI         3083  CCCGGG               CCC^GGG
XcmI         2199  CCAGACCCACCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        3083  CCCGGG               C^CCGGG
XmaI         3083  CCCGGG               C^CCGGG
XmnI          782  GAAGAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AflIII       1964  ACATGT               A^C[GA][CT]GT
             2579  ACATGT              
AspI          322  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2880  GACCATGTC           
AvaI         3083  CCCGGG               C^[CT]CG[GA]G
             3264  CTCGGG              
BalI         2069  TGGCCA               TGG^CCA
             2211  TGGCCA              
BbsI          238  GAAGAC               GAAGAC(2/6)
              319  GAAGAC              
BclI         2147  TGATCA               T^GATCA
             2706  TGATCA              
BpuAI         238  GAAGAC               GAAGAC(2/6)
              319  GAAGAC              
BsoBI        3083  CCCGGG               C^[CT]CG[GA]G
             3264  CTCGGG              
Bsp1407I     2582  TGTACA               T^GTACA
             3419  TGTACA              
BspLU11I     1964  ACATGT               A^CATGT
             2579  ACATGT              
BsrGI        2582  TGTACA               T^GTACA
             3419  TGTACA              
DraIII         32  CACCGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2935  CACCATGTG           
EcoNI         398  CCTTGGGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              498  CCTGGGGGAGG         
FbaI         2147  TGATCA               T^GATCA
             2706  TGATCA              
MluNI        2069  TGGCCA               TGG^CCA
             2211  TGGCCA              
MscI         2069  TGGCCA               TGG^CCA
             2211  TGGCCA              
NaeI          197  GCCGGC               GCC^GGC
             2240  GCCGGC              
NgoMIV        197  GCCGGC               G^CCGGC
             2240  GCCGGC              
PflFI         322  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2880  GACCATGTC           
PstI         2355  CTGCAG               CTGCA^G
             3307  CTGCAG              
ScaI          548  AGTACT               AGT^ACT
             3311  AGTACT              
SspBI        2582  TGTACA               T^GTACA
             3419  TGTACA              
StuI          395  AGGCCT               AGG^CCT
             3134  AGGCCT              
Tsp45I       1904  GTGAC                ^GT[GC]AC
             2849  GTCAC               
Tth111I       322  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2880  GACCATGTC           


#########################################
# 3 OR MORE CUT ENZYMES (   97 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApaI        ApoI        AspHI       AvaII       
BanII       BbvI        BcnI        BfaI        BmyI        
BpmI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrDI       BsrFI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HindIII     HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NcoI        NdeI        NdeII       
NlaIII      NlaIV       NspI        PleI        PpuMI       
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp509I     TspRI       
TthHB8I     XhoII       
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