Restriction map for
pF1KE0394
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# NO CUT ENZYMES ( 103 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI AocI
Aor51HI AscI AspEI AviII AvrII
BamHI BbeI BcgI BciVI BfrI
BglII BlnI BlpI Bpu1102I BsaHI
BsaI BseAI BsgI BsiWI BsmI
BspDI BspEI BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV FauI
FseI FspI HgaI Hin1I HpaI
KasI KspI MfeI MluI MroI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI
PaeR7I PinAI PmeI PshAI Psp1406I
PstI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SnaBI Sse8387I
SwaI XcmI XhoI
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# ONE CUT ENZYMES ( 40 enzymes) #
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Alw44I 1134 GTGCAC G^TGCAC
ApaLI 1134 GTGCAC G^TGCAC
Asp700I 2521 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2107 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 286 CACGTG CAC^GTG
BbsI 2382 GAAGAC GAAGAC(2/6)
BpuAI 2382 GAAGAC GAAGAC(2/6)
BsaAI 286 CACGTG [CT]AC^GT[GA]
BsaBI 2040 GATTCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 420 TCCGGT [AT]^CCGG[AT]
BsiEI 2988 CGGTCG CG[GA][CT]^CG
BsmBI 1320 GAGACG CGTCTC(1/5)
BspHI 839 TCATGA T^CATGA
BsrDI 125 GCAATG GCAATG(2/0)
BstBI 1834 TTCGAA TT^CGAA
BstYI 191 AGATCC [GA]^GATC[CT]
DsaI 87 CCATGG C^C[GA][CT]GG
EcoNI 2866 CCTTTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2404 GAATTC G^AATTC
EcoT22I 336 ATGCAT ATGCA^T
HaeII 2933 AGCGCC [GA]GCGC^[CT]
MamI 2040 GATTCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 191 AGATCC [GA]^GATC[CT]
NcoI 87 CCATGG C^CATGG
NsiI 336 ATGCAT ATGCA^T
NspV 1834 TTCGAA TT^CGAA
PflFI 2107 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 286 CACGTG CAC^GTG
PmlI 286 CACGTG CAC^GTG
PpuMI 2690 GGGACCT [GA]G^G[AT]CC[CT]
RcaI 839 TCATGA T^CATGA
SfcI 753 CTACAG C^T[GA][CT]AG
SfuI 1834 TTCGAA TT^CGAA
SmaI 986 CCCGGG CCC^GGG
Tth111I 2107 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1719 TCTAGA T^CTAGA
XhoII 191 AGATCC [GA]^GATC[CT]
XmaCI 986 CCCGGG C^CCGGG
XmaI 986 CCCGGG C^CCGGG
XmnI 2521 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 33 enzymes) #
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Acc65I 1611 GGTACC G^GTACC
1728 GGTACC
AlwNI 2290 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
2932 CAGCGCCTG
ApaI 1576 GGGCCC GGGCC^C
2494 GGGCCC
AseI 433 ATTAAT AT^TAAT
517 ATTAAT
AsnI 433 ATTAAT AT^TAAT
517 ATTAAT
Asp718I 1611 GGTACC G^GTACC
1728 GGTACC
AvaI 986 CCCGGG C^[CT]CG[GA]G
1526 CTCGGG
BalI 2969 TGGCCA TGG^CCA
3140 TGGCCA
BbvCI 1891 CCTCAGC CCTCAGC(-5/-2)
2929 CCTCAGC
BclI 260 TGATCA T^GATCA
849 TGATCA
BglI 146 GCCTAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
985 GCCCGGGAGGC
BsoBI 986 CCCGGG C^[CT]CG[GA]G
1526 CTCGGG
Bsp1407I 879 TGTACA T^GTACA
2951 TGTACA
BsrGI 879 TGTACA T^GTACA
2951 TGTACA
BstAPI 1255 GCAGTTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1666 GCATACTGTGC
CfoI 523 GCGC GCG^C
2934 GCGC
DraII 1575 GGGGCCC [GA]G^G[ACGT]CC[CT]
2690 GGGACCT
EaeI 2969 TGGCCA [CT]^GGCC[GA]
3140 TGGCCA
EcoO109I 1575 GGGGCCC [GA]G^G[ACGT]CC[CT]
2690 GGGACCT
FbaI 260 TGATCA T^GATCA
849 TGATCA
HhaI 523 GCGC GCG^C
2934 GCGC
HinP1I 523 GCGC G^CGC
2934 GCGC
KpnI 1611 GGTACC GGTAC^C
1728 GGTACC
MluNI 2969 TGGCCA TGG^CCA
3140 TGGCCA
MscI 2969 TGGCCA TGG^CCA
3140 TGGCCA
PflMI 1633 CCACCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2289 CCAGATTCTGG
PshBI 433 ATTAAT AT^TAAT
517 ATTAAT
PvuII 1109 CAGCTG CAG^CTG
2552 CAGCTG
SpeI 2505 ACTAGT A^CTAGT
3153 ACTAGT
SphI 1344 GCATGC GCATG^C
3324 GCATGC
SspBI 879 TGTACA T^GTACA
2951 TGTACA
SspI 61 AATATT AAT^ATT
502 AATATT
Van91I 1633 CCACCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2289 CCAGATTCTGG
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# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AspHI AvaII BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspLU11I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I