Restriction map for pF1KE0394
 (Linear) (Six-base) MAPSORT of: KIEE0394.seq  Check: 4038  from: 1  to: 3360



KIEE0394.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:13  ..

Acc65I G'GTAC_C

Cuts at:      0    1611    1728    3360 
   Size:       1611     117    1632

  Fragments arranged by size: 

                1632    1611     117
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1984    2207    3360 
   Size:       1984     223    1153

  Fragments arranged by size: 

                1984    1153     223
 
AflIII A'CryG_T

Cuts at:      0     285    1417    2956    3059    3360 
   Size:        285    1132    1539     103     301

  Fragments arranged by size: 

                1539    1132     301     285     103
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2159    3360 
   Size:       2159    1201
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2295    2937    3360 
   Size:       2295     642     423

  Fragments arranged by size: 

                2295     642     423
 
ApaI G_GGCC'C

Cuts at:      0    1580    2498    3360 
   Size:       1580     918     862

  Fragments arranged by size: 

                1580     918     862
 
ApaLI G'TGCA_C

Cuts at:      0    1134    3360 
   Size:       1134    2226
 
ApoI r'AATT_y

Cuts at:      0     466     600    1562    2396    2404    2701    3360 
   Size:        466     134     962     834       8     297     659

  Fragments arranged by size: 

                 962     834     659     466     297     134       8
 
AseI AT'TA_AT

Cuts at:      0     434     518    3360 
   Size:        434      84    2842

  Fragments arranged by size: 

                2842     434      84
 
AvaI C'yCGr_G

Cuts at:      0     986    1526    3360 
   Size:        986     540    1834

  Fragments arranged by size: 

                1834     986     540
 
BanI G'GyrC_C

Cuts at:      0    1377    1611    1728    3198    3360 
   Size:       1377     234     117    1470     162

  Fragments arranged by size: 

                1470    1377     234     162     117
 
BanII G_rGCy'C

Cuts at:      0     987    1391    1580    2498    3360 
   Size:        987     404     189     918     862

  Fragments arranged by size: 

                 987     918     862     404     189
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2389    3360 
   Size:       2389     971
 
BbvCI CC'TCA_GC

Cuts at:      0    1892    2930    3360 
   Size:       1892    1038     430

  Fragments arranged by size: 

                1892    1038     430
 
BclI T'GATC_A

Cuts at:      0     260     849    3360 
   Size:        260     589    2511

  Fragments arranged by size: 

                2511     589     260
 
BfrBI ATG'CAT

Cuts at:      0     338    3360 
   Size:        338    3022
 
BglI GCCn_nnn'nGGC

Cuts at:      0     152     991    3360 
   Size:        152     839    2369

  Fragments arranged by size: 

                2369     839     152
 
Bme1580I G_kGCm'C

Cuts at:      0    1138    1580    2498    3360 
   Size:       1138     442     918     862

  Fragments arranged by size: 

                1138     918     862     442
 
BmgBI CAC'GTC

Cuts at:      0    1324    3360 
   Size:       1324    2036
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    3176    3360 
   Size:       3176     184
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      19     123     263     651     662    1208    1471    1718 
   Size:         19     104     140     388      11     546     263     247

Cuts at:   1718    2440    3360 
   Size:        722     920

  Fragments arranged by size: 

                 920     722     546     388     263     247     140     104
                  19      11
 
Bpu10I CC'TnA_GC

Cuts at:      0    1265    1892    2930    3360 
   Size:       1265     627    1038     430

  Fragments arranged by size: 

                1265    1038     627     430
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     481    1160    1286    1615    3360 
   Size:        481     679     126     329    1745

  Fragments arranged by size: 

                1745     679     481     329     126
 
BsaAI yAC'GTr

Cuts at:      0     288    3360 
   Size:        288    3072
 
BsaBI GATnn'nnATC

Cuts at:      0    2044    3360 
   Size:       2044    1316
 
BsaWI w'CCGG_w

Cuts at:      0     420    3360 
   Size:        420    2940
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     605     635    1469    1499    1955    1985    3360 
   Size:        605      30     834      30     456      30    1375

  Fragments arranged by size: 

                1375     834     605     456      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     265    1447    1738    2665    3226    3360 
   Size:        265    1182     291     927     561     134

  Fragments arranged by size: 

                1182     927     561     291     265     134
 
BsiEI CG_ry'CG

Cuts at:      0    2991    3360 
   Size:       2991     369
 
BsiHKAI G_wGCw'C

Cuts at:      0       8     637     740    1138    1487    1677    1970    3360 
   Size:          8     629     103     398     349     190     293    1390

  Fragments arranged by size: 

                1390     629     398     349     293     190     103       8
            
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1314    3360 
   Size:       1314    2046
 
Bsp1286I G_dGCh'C

Cuts at:      0       8     637     740     987    1138    1391    1487    1580 
   Size:          8     629     103     247     151     253      96      93

Cuts at:   1580    1677    1970    2498    3360 
   Size:         97     293     528     862

  Fragments arranged by size: 

                 862     629     528     293     253     247     151     103
                  97      96      93       8
 
BspHI T'CATG_A

Cuts at:      0     839    3360 
   Size:        839    2521
 
BsrDI GCAATG_nn'

Cuts at:      0     132    3360 
   Size:        132    3228
 
BsrGI T'GTAC_A

Cuts at:      0     879    2951    3360 
   Size:        879    2072     409

  Fragments arranged by size: 

                2072     879     409
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1261    1672    3360 
   Size:       1261     411    1688

  Fragments arranged by size: 

                1688    1261     411
 
BstBI TT'CG_AA

Cuts at:      0    1835    3360 
   Size:       1835    1525
 
BstYI r'GATC_y

Cuts at:      0     191    3360 
   Size:        191    3169
 
BtgI C'CryG_G

Cuts at:      0      87    3360 
   Size:         87    3273
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2019    3360 
   Size:       2019    1341
 
BtsI GCAGTG_nn'

Cuts at:      0      44    1217    2914    3360 
   Size:         44    1173    1697     446

  Fragments arranged by size: 

                1697    1173     446      44
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1830    1865    3360 
   Size:       1830      35    1495

  Fragments arranged by size: 

                1830    1495      35
 
EaeI y'GGCC_r

Cuts at:      0    2969    3140    3360 
   Size:       2969     171     220

  Fragments arranged by size: 

                2969     220     171
 
EarI CTCTTCn'nnn_

Cuts at:      0    1545    2812    3118    3360 
   Size:       1545    1267     306     242

  Fragments arranged by size: 

                1545    1267     306     242
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      19     123     263     651     662    1208    1471    1718 
   Size:         19     104     140     388      11     546     263     247

Cuts at:   1718    1984    2207    2440    3360 
   Size:        266     223     233     920

  Fragments arranged by size: 

                 920     546     388     266     263     247     233     223
                 140     104      19      11
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2870    3360 
   Size:       2870     490
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1576    2691    3360 
   Size:       1576    1115     669

  Fragments arranged by size: 

                1576    1115     669
 
EcoRI G'AATT_C

Cuts at:      0    2404    3360 
   Size:       2404     956
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     421    2014    3360 
   Size:        421    1593    1346

  Fragments arranged by size: 

                1593    1346     421
 
HaeII r_GCGC'y

Cuts at:      0    2937    3360 
   Size:       2937     423
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     261     283     293     315     568     600    2069    2101 
   Size:        261      22      10      22     253      32    1469      32

Cuts at:   2101    2785    2817    3006    3038    3360 
   Size:        684      32     189      32     322

  Fragments arranged by size: 

                1469     684     322     261     253     189      32      32
                  32      32      22      22      10
 
HincII GTy'rAC

Cuts at:      0      84    2106    2202    2336    2460    3360 
   Size:         84    2022      96     134     124     900

  Fragments arranged by size: 

                2022     900     134     124      96      84
 
HindIII A'AGCT_T

Cuts at:      0     398    1354    2561    3096    3360 
   Size:        398     956    1207     535     264

  Fragments arranged by size: 

                1207     956     535     398     264
 
KpnI G_GTAC'C

Cuts at:      0    1615    1732    3360 
   Size:       1615     117    1628

  Fragments arranged by size: 

                1628    1615     117
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2643    3360 
   Size:       2643     717
 
MscI TGG'CCA

Cuts at:      0    2971    3142    3360 
   Size:       2971     171     218

  Fragments arranged by size: 

                2971     218     171
 
MslI CAynn'nnrTG

Cuts at:      0     343    1880    2048    3360 
   Size:        343    1537     168    1312

  Fragments arranged by size: 

                1537    1312     343     168
 
MspA1I CmG'CkG

Cuts at:      0     316    1111    2554    3360 
   Size:        316     795    1443     806

  Fragments arranged by size: 

                1443     806     795     316
 
NcoI C'CATG_G

Cuts at:      0      87    3360 
   Size:         87    3273
 
NsiI A_TGCA'T

Cuts at:      0     340    3360 
   Size:        340    3020
 
NspI r_CATG'y

Cuts at:      0    1348    1421    2906    2960    3063    3328    3360 
   Size:       1348      73    1485      54     103     265      32

  Fragments arranged by size: 

                1485    1348     265     103      73      54      32
 
PciI A'CATG_T

Cuts at:      0    1417    2956    3059    3360 
   Size:       1417    1539     103     301

  Fragments arranged by size: 

                1539    1417     301     103
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1639    2295    3360 
   Size:       1639     656    1065

  Fragments arranged by size: 

                1639    1065     656
 
PfoI T'CCnGG_A

Cuts at:      0     279     890    1487    1734    3360 
   Size:        279     611     597     247    1626

  Fragments arranged by size: 

                1626     611     597     279     247
 
PmlI CAC'GTG

Cuts at:      0     288    3360 
   Size:        288    3072
 
PpuMI rG'GwC_Cy

Cuts at:      0    2691    3360 
   Size:       2691     669
 
PspOMI G'GGCC_C

Cuts at:      0    1576    2494    3360 
   Size:       1576     918     866

  Fragments arranged by size: 

                1576     918     866
 
PvuII CAG'CTG

Cuts at:      0    1111    2554    3360 
   Size:       1111    1443     806

  Fragments arranged by size: 

                1443    1111     806
 
SfcI C'TryA_G

Cuts at:      0     753    3360 
   Size:        753    2607
 
SmaI CCC'GGG

Cuts at:      0     988    3360 
   Size:        988    2372
 
SmlI C'TyrA_G

Cuts at:      0     496    1175    1301    1594    3360 
   Size:        496     679     126     293    1766

  Fragments arranged by size: 

                1766     679     496     293     126
 
SpeI A'CTAG_T

Cuts at:      0    2505    3153    3360 
   Size:       2505     648     207

  Fragments arranged by size: 

                2505     648     207
 
SphI G_CATG'C

Cuts at:      0    1348    3328    3360 
   Size:       1348    1980      32

  Fragments arranged by size: 

                1980    1348      32
 
SspI AAT'ATT

Cuts at:      0      63     504    3360 
   Size:         63     441    2856

  Fragments arranged by size: 

                2856     441      63
 
StuI AGG'CCT

Cuts at:      0     484    1270    1293    1760    2809    3360 
   Size:        484     786      23     467    1049     551

  Fragments arranged by size: 

                1049     786     551     484     467      23
 
StyI C'CwwG_G

Cuts at:      0      87    1288    1382    3360 
   Size:         87    1201      94    1978

  Fragments arranged by size: 

                1978    1201      94      87
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     707    1408    2302    2653    2977    3186    3360 
   Size:        707     701     894     351     324     209     174

  Fragments arranged by size: 

                 894     707     701     351     324     209     174
 
TatI w'GTAC_w

Cuts at:      0     879    2951    3266    3360 
   Size:        879    2072     315      94

  Fragments arranged by size: 

                2072     879     315      94
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      51      83     680     712    3360 
   Size:         51      32     597      32    2648

  Fragments arranged by size: 

                2648     597      51      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2110    3360 
   Size:       2110    1250
 
XbaI T'CTAG_A

Cuts at:      0    1719    3360 
   Size:       1719    1641
 
XmaI C'CCGG_G

Cuts at:      0     986    3360 
   Size:        986    2374
 
XmnI GAAnn'nnTTC

Cuts at:      0    2525    3360 
   Size:       2525     835
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII     AlfI    AlwNI     ApaI    ApaLI     ApoI
     AseI     AvaI     BanI    BanII     BbsI    BbvCI     BclI    BfrBI
     BglI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaWI    BsaXI    BseRI    BsiEI  BsiHKAI    BsmBI Bsp1286I
    BspHI    BsrDI    BsrGI   BstAPI    BstBI    BstYI     BtgI    BtgZI
     BtsI    CspCI     EaeI     EarI  Eco57MI    EcoNI EcoO109I    EcoRI
     FalI    HaeII    Hin4I   HincII  HindIII     KpnI     MmeI     MscI
     MslI   MspA1I     NcoI     NsiI     NspI     PciI    PflMI     PfoI
     PmlI    PpuMI   PspOMI    PvuII     SfcI     SmaI     SmlI     SpeI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AloI     AscI    AsiSI    AvrII     BaeI    BamHI     BbeI
     BcgI    BciVI    BglII     BlpI     BmtI     BplI     BsaI    BsaHI
    BseYI     BsgI    BsiWI     BsmI    BspEI    BspMI    BsrBI    BsrFI
   BssHII    BssSI   BstEII    BstXI  BstZ17I   Bsu36I     ClaI     DraI
   DraIII     DrdI     EagI     EciI  EcoICRI    EcoRV     FseI     FspI
    FspAI     HpaI     KasI     MfeI     MluI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     PacI     PasI     PmeI     PpiI
    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SrfI     SwaI     XcmI     XhoI
     ZraI
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