Restriction map for
pF1KE0392
(Linear) (Six-base) MAPSORT of: KIEE0392.seq Check: 2362 from: 1 to: 2793
KIEE0392.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2188 2737 2793
Size: 2188 549 56
Fragments arranged by size:
2188 549 56
AccI GT'mk_AC
Cuts at: 0 1297 2681 2793
Size: 1297 1384 112
Fragments arranged by size:
1384 1297 112
AclI AA'CG_TT
Cuts at: 0 761 2793
Size: 761 2032
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 485 2024 2201 2401 2793
Size: 485 1539 177 200 392
Fragments arranged by size:
1539 485 392 200 177
AflIII A'CryG_T
Cuts at: 0 1147 2793
Size: 1147 1646
AleI CACnn'nnGTG
Cuts at: 0 746 1720 2793
Size: 746 974 1073
Fragments arranged by size:
1073 974 746
AlwNI CAG_nnn'CTG
Cuts at: 0 1590 2793
Size: 1590 1203
ApaI G_GGCC'C
Cuts at: 0 1893 2793
Size: 1893 900
ApaLI G'TGCA_C
Cuts at: 0 1493 2793
Size: 1493 1300
ApoI r'AATT_y
Cuts at: 0 882 2793
Size: 882 1911
AvaI C'yCGr_G
Cuts at: 0 167 733 1083 2058 2793
Size: 167 566 350 975 735
Fragments arranged by size:
975 735 566 350 167
BamHI G'GATC_C
Cuts at: 0 1535 2793
Size: 1535 1258
BanI G'GyrC_C
Cuts at: 0 6 162 244 1731 2470 2503 2602 2608
Size: 6 156 82 1487 739 33 99 6
Cuts at: 2608 2793
Size: 185
Fragments arranged by size:
1487 739 185 156 99 82 33 6
6
BanII G_rGCy'C
Cuts at: 0 284 1893 2587 2793
Size: 284 1609 694 206
Fragments arranged by size:
1609 694 284 206
BbeI G_GCGC'C
Cuts at: 0 10 166 2793
Size: 10 156 2627
Fragments arranged by size:
2627 156 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 1484 2100 2793
Size: 1484 616 693
Fragments arranged by size:
1484 693 616
BciVI GTATCCnnnnn_n'
Cuts at: 0 693 2793
Size: 693 2100
BglII A'GATC_T
Cuts at: 0 44 292 2793
Size: 44 248 2501
Fragments arranged by size:
2501 248 44
Bme1580I G_kGCm'C
Cuts at: 0 1497 1893 2663 2793
Size: 1497 396 770 130
Fragments arranged by size:
1497 770 396 130
BmgBI CAC'GTC
Cuts at: 0 543 828 2793
Size: 543 285 1965
Fragments arranged by size:
1965 543 285
BmrI ACTGGGnnnn_n'
Cuts at: 0 386 2634 2793
Size: 386 2248 159
Fragments arranged by size:
2248 386 159
BmtI G_CTAG'C
Cuts at: 0 2137 2793
Size: 2137 656
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1927 2793
Size: 1927 866
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 186 337 2793
Size: 25 161 151 2456
Fragments arranged by size:
2456 161 151 25
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1412 1810 1897 2017 2125 2793
Size: 1412 398 87 120 108 668
Fragments arranged by size:
1412 668 398 120 108 87
BsaI GGTCTCn'nnnn_
Cuts at: 0 982 1571 1607 2274 2399 2457 2793
Size: 982 589 36 667 125 58 336
Fragments arranged by size:
982 667 589 336 125 58 36
BsaAI yAC'GTr
Cuts at: 0 744 2658 2793
Size: 744 1914 135
Fragments arranged by size:
1914 744 135
BsaBI GATnn'nnATC
Cuts at: 0 1330 2793
Size: 1330 1463
BsaHI Gr'CG_yC
Cuts at: 0 7 163 2557 2793
Size: 7 156 2394 236
Fragments arranged by size:
2394 236 156 7
BsaWI w'CCGG_w
Cuts at: 0 477 842 2793
Size: 477 365 1951
Fragments arranged by size:
1951 477 365
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2254 2284 2793
Size: 2254 30 509
Fragments arranged by size:
2254 509 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 124 292 1277 1415 2793
Size: 124 168 985 138 1378
Fragments arranged by size:
1378 985 168 138 124
BseYI C'CCAG_C
Cuts at: 0 2317 2742 2793
Size: 2317 425 51
Fragments arranged by size:
2317 425 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1846 2793
Size: 1846 947
BsiHKAI G_wGCw'C
Cuts at: 0 284 1497 2587 2793
Size: 284 1213 1090 206
Fragments arranged by size:
1213 1090 284 206
BsmI GAATG_Cn'
Cuts at: 0 1806 2793
Size: 1806 987
BsmBI CGTCTCn'nnnn_
Cuts at: 0 204 818 1898 2142 2793
Size: 204 614 1080 244 651
Fragments arranged by size:
1080 651 614 244 204
Bsp1286I G_dGCh'C
Cuts at: 0 284 1497 1893 2587 2663 2793
Size: 284 1213 396 694 76 130
Fragments arranged by size:
1213 694 396 284 130 76
BspEI T'CCGG_A
Cuts at: 0 477 842 2793
Size: 477 365 1951
Fragments arranged by size:
1951 477 365
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1589 2180 2188 2737 2793
Size: 1589 591 8 549 56
Fragments arranged by size:
1589 591 549 56 8
BsrBI CCG'CTC
Cuts at: 0 1739 2793
Size: 1739 1054
BsrDI GCAATG_nn'
Cuts at: 0 1638 2703 2760 2793
Size: 1638 1065 57 33
Fragments arranged by size:
1638 1065 57 33
BsrFI r'CCGG_y
Cuts at: 0 2500 2605 2793
Size: 2500 105 188
Fragments arranged by size:
2500 188 105
BssSI C'ACGA_G
Cuts at: 0 2077 2263 2793
Size: 2077 186 530
Fragments arranged by size:
2077 530 186
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2658 2793
Size: 2658 135
BstEII G'GTnAC_C
Cuts at: 0 252 505 652 1761 2793
Size: 252 253 147 1109 1032
Fragments arranged by size:
1109 1032 253 252 147
BstXI CCAn_nnnn'nTGG
Cuts at: 0 153 1722 1773 1945 2553 2793
Size: 153 1569 51 172 608 240
Fragments arranged by size:
1569 608 240 172 153 51
BstYI r'GATC_y
Cuts at: 0 44 292 1535 1682 2793
Size: 44 248 1243 147 1111
Fragments arranged by size:
1243 1111 248 147 44
BtgI C'CryG_G
Cuts at: 0 257 1944 2279 2612 2793
Size: 257 1687 335 333 181
Fragments arranged by size:
1687 335 333 257 181
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 126 2507 2793
Size: 126 2381 286
Fragments arranged by size:
2381 286 126
BtsI GCAGTG_nn'
Cuts at: 0 671 1315 1784 1859 2093 2793
Size: 671 644 469 75 234 700
Fragments arranged by size:
700 671 644 469 234 75
ClaI AT'CG_AT
Cuts at: 0 371 2793
Size: 371 2422
DraIII CAC_nnn'GTG
Cuts at: 0 2220 2793
Size: 2220 573
EaeI y'GGCC_r
Cuts at: 0 1472 1947 1964 1991 2090 2793
Size: 1472 475 17 27 99 703
Fragments arranged by size:
1472 703 475 99 27 17
EarI CTCTTCn'nnn_
Cuts at: 0 464 605 2287 2375 2793
Size: 464 141 1682 88 418
Fragments arranged by size:
1682 464 418 141 88
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 193 2550 2793
Size: 193 2357 243
Fragments arranged by size:
2357 243 193
EcoICRI GAG'CTC
Cuts at: 0 282 2585 2793
Size: 282 2303 208
Fragments arranged by size:
2303 282 208
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 186 337 485 2024 2201 2401 2793
Size: 25 161 151 148 1539 177 200 392
Fragments arranged by size:
1539 392 200 177 161 151 148 25
EcoO109I rG'GnC_Cy
Cuts at: 0 150 1890 2739 2793
Size: 150 1740 849 54
Fragments arranged by size:
1740 849 150 54
EcoRI G'AATT_C
Cuts at: 0 882 2793
Size: 882 1911
FspI TGC'GCA
Cuts at: 0 1879 2793
Size: 1879 914
FspAI rTGC'GCAy
Cuts at: 0 1879 2793
Size: 1879 914
HaeII r_GCGC'y
Cuts at: 0 10 166 2627 2793
Size: 10 156 2461 166
Fragments arranged by size:
2461 166 156 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 179 211 847 879 1108 1140 1394 1426
Size: 179 32 636 32 229 32 254 32
Cuts at: 1426 1774 1806 2569 2601 2793
Size: 348 32 763 32 192
Fragments arranged by size:
763 636 348 254 229 192 179 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1214 2793
Size: 1214 1579
HindIII A'AGCT_T
Cuts at: 0 467 2793
Size: 467 2326
KasI G'GCGC_C
Cuts at: 0 6 162 2793
Size: 6 156 2631
Fragments arranged by size:
2631 156 6
MscI TGG'CCA
Cuts at: 0 1949 2793
Size: 1949 844
MslI CAynn'nnrTG
Cuts at: 0 746 1118 1720 2533 2793
Size: 746 372 602 813 260
Fragments arranged by size:
813 746 602 372 260
MspA1I CmG'CkG
Cuts at: 0 242 259 1587 2095 2281 2477 2719 2793
Size: 242 17 1328 508 186 196 242 74
Fragments arranged by size:
1328 508 242 242 196 186 74 17
NaeI GCC'GGC
Cuts at: 0 2607 2793
Size: 2607 186
NarI GG'CG_CC
Cuts at: 0 7 163 2793
Size: 7 156 2630
Fragments arranged by size:
2630 156 7
NcoI C'CATG_G
Cuts at: 0 1944 2612 2793
Size: 1944 668 181
Fragments arranged by size:
1944 668 181
NgoMIV G'CCGG_C
Cuts at: 0 2605 2793
Size: 2605 188
NheI G'CTAG_C
Cuts at: 0 2133 2793
Size: 2133 660
NruI TCG'CGA
Cuts at: 0 605 2793
Size: 605 2188
NspI r_CATG'y
Cuts at: 0 1151 2793
Size: 1151 1642
PasI CC'CwG_GG
Cuts at: 0 2592 2793
Size: 2592 201
PciI A'CATG_T
Cuts at: 0 1147 2793
Size: 1147 1646
PflMI CCAn_nnn'nTGG
Cuts at: 0 2749 2793
Size: 2749 44
PfoI T'CCnGG_A
Cuts at: 0 1067 1937 2793
Size: 1067 870 856
Fragments arranged by size:
1067 870 856
PmlI CAC'GTG
Cuts at: 0 2658 2793
Size: 2658 135
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 77 109 2793
Size: 77 32 2684
Fragments arranged by size:
2684 77 32
PpuMI rG'GwC_Cy
Cuts at: 0 150 2793
Size: 150 2643
PshAI GACnn'nnGTC
Cuts at: 0 1793 2793
Size: 1793 1000
PspOMI G'GGCC_C
Cuts at: 0 1889 2793
Size: 1889 904
PstI C_TGCA'G
Cuts at: 0 503 1586 2252 2654 2793
Size: 503 1083 666 402 139
Fragments arranged by size:
1083 666 503 402 139
PvuII CAG'CTG
Cuts at: 0 1587 2719 2793
Size: 1587 1132 74
Fragments arranged by size:
1587 1132 74
SacI G_AGCT'C
Cuts at: 0 284 2587 2793
Size: 284 2303 206
Fragments arranged by size:
2303 284 206
SacII CC_GC'GG
Cuts at: 0 260 2282 2793
Size: 260 2022 511
Fragments arranged by size:
2022 511 260
SbfI CC_TGCA'GG
Cuts at: 0 503 2793
Size: 503 2290
SexAI A'CCwGG_T
Cuts at: 0 1757 2793
Size: 1757 1036
SfcI C'TryA_G
Cuts at: 0 499 767 1298 1582 1815 2248 2650 2793
Size: 499 268 531 284 233 433 402 143
Fragments arranged by size:
531 499 433 402 284 268 233 143
SfoI GGC'GCC
Cuts at: 0 8 164 2793
Size: 8 156 2629
Fragments arranged by size:
2629 156 8
SmaI CCC'GGG
Cuts at: 0 169 1085 2793
Size: 169 916 1708
Fragments arranged by size:
1708 916 169
SmlI C'TyrA_G
Cuts at: 0 1427 1825 1912 2032 2140 2793
Size: 1427 398 87 120 108 653
Fragments arranged by size:
1427 653 398 120 108 87
SspI AAT'ATT
Cuts at: 0 908 2793
Size: 908 1885
StyI C'CwwG_G
Cuts at: 0 146 248 1944 2612 2793
Size: 146 102 1696 668 181
Fragments arranged by size:
1696 668 181 146 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 405 1362 2793
Size: 405 957 1431
Fragments arranged by size:
1431 957 405
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1337 1369 2254 2286 2793
Size: 1337 32 885 32 507
Fragments arranged by size:
1337 885 507 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1672 2793
Size: 1672 1121
XbaI T'CTAG_A
Cuts at: 0 327 597 918 2793
Size: 327 270 321 1875
Fragments arranged by size:
1875 327 321 270
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 970 2793
Size: 970 1823
XmaI C'CCGG_G
Cuts at: 0 167 1083 2793
Size: 167 916 1710
Fragments arranged by size:
1710 916 167
XmnI GAAnn'nnTTC
Cuts at: 0 89 2793
Size: 89 2704
Enzymes that do cut:
AarI AccI AclI AcuI AflIII AleI AlwNI ApaI
ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI
BciVI BglII Bme1580I BmgBI BmrI BmtI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI DraIII EaeI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I
HincII HindIII KasI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NruI NspI PasI PciI PflMI
PfoI PmlI PpiI PpuMI PshAI PspOMI PstI PvuII
SacI SacII SbfI SexAI SfcI SfoI SmaI SmlI
SspI StyI TaqII TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AflII AgeI AhdI AlfI AloI
AscI AseI AsiSI AvrII BaeI BbvCI BcgI BclI
BfrBI BglI BlpI Bpu10I BsiEI BsiWI BspHI BsrGI
BssHII BstBI BstZ17I Bsu36I CspCI DraI DrdI EagI
EcoNI EcoRV FalI FseI HpaI KpnI MfeI MluI
MmeI NdeI NotI NsiI PacI PmeI PsiI PspXI
PsrI PvuI RsrII SalI SanDI SapI ScaI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI StuI SwaI
TatI XhoI ZraI