Restriction map for
pF1KE0380
(Circular) (Six-base) MAPSORT of: pF1KE0380.seq Check: 9008 from: 1 to: 4856
pF1KE0380 4856 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 1894 1894
Size: 4856
Acc65I G'GTAC_C
Cuts at: 1872 1872
Size: 4856
AclI AA'CG_TT
Cuts at: 4696 4696
Size: 4856
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 676 1280 2681 3113 3860 4128 676
Size: 604 1401 432 747 268 1404
Fragments arranged by size:
1404 1401 747 604 432 268
AfeI AGC'GCT
Cuts at: 466 664 466
Size: 198 4658
AflIII A'CryG_T
Cuts at: 1577 2208 3312 4289 1577
Size: 631 1104 977 2144
Fragments arranged by size:
2144 1104 977 631
AgeI A'CCGG_T
Cuts at: 3244 3244
Size: 4856
AhdI GACnn_n'nnGTC
Cuts at: 1525 1525
Size: 4856
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 460 882 460
Size: 422 4434
AlwNI CAG_nnn'CTG
Cuts at: 979 2161 3728 979
Size: 1182 1567 2107
Fragments arranged by size:
2107 1567 1182
ApaLI G'TGCA_C
Cuts at: 3626 3626
Size: 4856
ApoI r'AATT_y
Cuts at: 1129 1276 1860 4209 4465 1129
Size: 147 584 2349 256 1520
Fragments arranged by size:
2349 1520 584 256 147
AseI AT'TA_AT
Cuts at: 20 20
Size: 4856
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4856
AvaI C'yCGr_G
Cuts at: 280 460 1876 280
Size: 180 1416 3260
Fragments arranged by size:
3260 1416 180
BamHI G'GATC_C
Cuts at: 839 1881 839
Size: 1042 3814
BanI G'GyrC_C
Cuts at: 315 1872 2286 2534 2569 315
Size: 1557 414 248 35 2602
Fragments arranged by size:
2602 1557 414 248 35
BanII G_rGCy'C
Cuts at: 211 741 929 1289 1870 2900 211
Size: 530 188 360 581 1030 2167
Fragments arranged by size:
2167 1030 581 530 360 188
BbeI G_GCGC'C
Cuts at: 2290 2538 2290
Size: 248 4608
BbvCI CC'TCA_GC
Cuts at: 427 427
Size: 4856
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1660 1694 1660
Size: 34 4822
BciVI GTATCCnnnnn_n'
Cuts at: 986 1415 1641 2754 3515 4856 986
Size: 429 226 1113 761 1341 986
Fragments arranged by size:
1341 1113 986 761 429 226
BclI T'GATC_A
Cuts at: 2376 2376
Size: 4856
BfrBI ATG'CAT
Cuts at: 1662 1662
Size: 4856
BglII A'GATC_T
Cuts at: 2371 4408 2371
Size: 2037 2819
BlpI GC'TnA_GC
Cuts at: 1968 1968
Size: 4856
Bme1580I G_kGCm'C
Cuts at: 871 1314 2481 2574 3630 871
Size: 443 1167 93 1056 2097
Fragments arranged by size:
2097 1167 1056 443 93
BmgBI CAC'GTC
Cuts at: 1580 1580
Size: 4856
BmrI ACTGGGnnnn_n'
Cuts at: 2068 2149 2484 4628 2068
Size: 81 335 2144 2296
Fragments arranged by size:
2296 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1141 3223 1141
Size: 2082 2774
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 841 969 1351 1438 841
Size: 128 382 87 4259
Fragments arranged by size:
4259 382 128 87
Bpu10I CC'TnA_GC
Cuts at: 427 427
Size: 4856
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 2032 2911 3403 3701 607
Size: 1425 879 492 298 1762
Fragments arranged by size:
1762 1425 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1031 4560 1031
Size: 3529 1327
BsaAI yAC'GTr
Cuts at: 965 2209 965
Size: 1244 3612
BsaBI GATnn'nnATC
Cuts at: 6 418 2370 4407 6
Size: 412 1952 2037 455
Fragments arranged by size:
2037 1952 455 412
BsaHI Gr'CG_yC
Cuts at: 754 2287 2535 3237 4733 754
Size: 1533 248 702 1496 877
Fragments arranged by size:
1533 1496 877 702 248
BsaWI w'CCGG_w
Cuts at: 2045 2566 3244 3518 3665 4076 2045
Size: 521 678 274 147 411 2825
Fragments arranged by size:
2825 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 154 184 1387 1417 154
Size: 30 1203 30 3593
Fragments arranged by size:
3593 1203 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 388 406 865 1130 388
Size: 18 459 265 4114
Fragments arranged by size:
4114 459 265 18
BseYI C'CCAG_C
Cuts at: 204 330 747 2281 3039 3616 4112 204
Size: 126 417 1534 758 577 496 948
Fragments arranged by size:
1534 948 758 577 496 417 126
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 430 1274 1788 391
Size: 39 844 514 3459
Fragments arranged by size:
3459 844 514 39
BsiEI CG_ry'CG
Cuts at: 104 159 310 435 2398 2444 3652 104
Size: 55 151 125 1963 46 1208 1308
Fragments arranged by size:
1963 1308 1208 151 125 55 46
BsiHKAI G_wGCw'C
Cuts at: 179 653 1289 1870 2648 2838 3630 179
Size: 474 636 581 778 190 792 1405
Fragments arranged by size:
1405 792 778 636 581 474 190
Bsp1286I G_dGCh'C
Cuts at: 179 211 653 741 871 929 1289 1314 1870
Size: 32 442 88 130 58 360 25 556
Cuts at: 1870 2481 2574 2648 2838 2900 3630 179
Size: 611 93 74 190 62 730 1405
Fragments arranged by size:
1405 730 611 556 442 360 190 130
93 88 74 62 58 32 25
BspEI T'CCGG_A
Cuts at: 4076 4076
Size: 4856
BspHI T'CATG_A
Cuts at: 4032 4032
Size: 4856
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1906 2120 2422 2803 1906
Size: 214 302 381 3959
Fragments arranged by size:
3959 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3148 3202 4686 4851 50
Size: 3098 54 1484 165 55
Fragments arranged by size:
3098 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1625 2768 1625
Size: 1143 3713
BsrFI r'CCGG_y
Cuts at: 294 312 318 2854 3035 3244 294
Size: 18 6 2536 181 209 1906
Fragments arranged by size:
2536 1906 209 181 18 6
BssHII G'CGCG_C
Cuts at: 335 335
Size: 4856
BssSI C'ACGA_G
Cuts at: 3127 3485 3127
Size: 358 4498
BstAPI GCAn_nnn'nTGC
Cuts at: 2233 2233
Size: 4856
BstBI TT'CG_AA
Cuts at: 902 3217 902
Size: 2315 2541
BstYI r'GATC_y
Cuts at: 1 839 1142 1717 1881 2348 2371 2706 2952
Size: 838 303 575 164 467 23 335 246
Cuts at: 2952 3964 4050 4062 4408 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 838 575 467 449 346 335 303
246 164 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1424 1616 1424
Size: 192 4664
BtgI C'CryG_G
Cuts at: 139 304 139
Size: 165 4691
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 785 1282 2806 2987 3150 785
Size: 497 1524 181 163 2491
Fragments arranged by size:
2491 1524 497 181 163
BtsI GCAGTG_nn'
Cuts at: 442 866 1156 2099 442
Size: 424 290 943 3199
Fragments arranged by size:
3199 943 424 290
DraI TTT'AAA
Cuts at: 1281 1855 1281
Size: 574 4282
DrdI GACnn_nn'nnGTC
Cuts at: 2562 3420 2562
Size: 858 3998
EaeI y'GGCC_r
Cuts at: 156 197 307 668 1191 1238 2441 2615 3006
Size: 41 110 361 523 47 1203 174 391
Cuts at: 3006 3033 4378 156
Size: 27 1345 634
Fragments arranged by size:
1345 1203 634 523 391 361 174 110
47 41 27
EagI C'GGCC_G
Cuts at: 156 307 2441 156
Size: 151 2134 2571
Fragments arranged by size:
2571 2134 151
EarI CTCTTCn'nnn_
Cuts at: 842 2879 3089 842
Size: 2037 210 2609
Fragments arranged by size:
2609 2037 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3064 3374 3520 4666 3064
Size: 310 146 1146 3254
Fragments arranged by size:
3254 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1287 1868 1287
Size: 581 4275
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 676 841 969 1280 1351 1438 2681 3113 3860
Size: 165 128 311 71 87 1243 432 747
Cuts at: 3860 4128 676
Size: 268 1404
Fragments arranged by size:
1404 1243 747 432 311 268 165 128
87 71
EcoO109I rG'GnC_Cy
Cuts at: 259 726 1995 259
Size: 467 1269 3120
Fragments arranged by size:
3120 1269 467
EcoRI G'AATT_C
Cuts at: 1860 1860
Size: 4856
EcoRV GAT'ATC
Cuts at: 603 603
Size: 4856
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 640 681 1407 1719 2875 640
Size: 41 726 312 1156 2621
Fragments arranged by size:
2621 1156 726 312 41
FspI TGC'GCA
Cuts at: 2637 2637
Size: 4856
HaeII r_GCGC'y
Cuts at: 135 169 187 468 666 2290 2538 3560 4539
Size: 34 18 281 198 1624 248 1022 979
Cuts at: 4539 135
Size: 452
Fragments arranged by size:
1624 1022 979 452 281 248 198 34
18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 424 456 1389 1421 1511 1543 1564 1596 1702
Size: 32 933 32 90 32 21 32 106
Cuts at: 1702 1734 2698 2728 2730 2760 424
Size: 32 964 30 2 30 2520
Fragments arranged by size:
2520 964 933 106 90 32 32 32
32 32 30 30 21 2
HincII GTy'rAC
Cuts at: 557 1895 557
Size: 1338 3518
KasI G'GCGC_C
Cuts at: 2286 2534 2286
Size: 248 4608
KpnI G_GTAC'C
Cuts at: 1876 1876
Size: 4856
MluI A'CGCG_T
Cuts at: 4289 4289
Size: 4856
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1054 3527 3711 1054
Size: 2473 184 2199
Fragments arranged by size:
2473 2199 184
MscI TGG'CCA
Cuts at: 670 1193 1240 2617 670
Size: 523 47 1377 2909
Fragments arranged by size:
2909 1377 523 47
MslI CAynn'nnrTG
Cuts at: 1176 1630 2845 2972 1176
Size: 454 1215 127 3060
Fragments arranged by size:
3060 1215 454 127
MspA1I CmG'CkG
Cuts at: 141 306 363 477 828 1258 1967 2281 2641
Size: 165 57 114 351 430 709 314 360
Cuts at: 2641 3654 3899 141
Size: 1013 245 1098
Fragments arranged by size:
1098 1013 709 430 360 351 314 245
165 114 57
NaeI GCC'GGC
Cuts at: 320 3037 320
Size: 2717 2139
NarI GG'CG_CC
Cuts at: 2287 2535 2287
Size: 248 4608
NgoMIV G'CCGG_C
Cuts at: 318 3035 318
Size: 2717 2139
NotI GC'GGCC_GC
Cuts at: 307 307
Size: 4856
NsiI A_TGCA'T
Cuts at: 1664 1664
Size: 4856
NspI r_CATG'y
Cuts at: 1420 1848 1909 2409 3316 1420
Size: 428 61 500 907 2960
Fragments arranged by size:
2960 907 500 428 61
PciI A'CATG_T
Cuts at: 3312 3312
Size: 4856
PflMI CCAn_nnn'nTGG
Cuts at: 1515 1515
Size: 4856
PfoI T'CCnGG_A
Cuts at: 785 1355 785
Size: 570 4286
PmeI GTTT'AAAC
Cuts at: 1855 1855
Size: 4856
PmlI CAC'GTG
Cuts at: 965 965
Size: 4856
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3995 4027 3995
Size: 32 4824
PpuMI rG'GwC_Cy
Cuts at: 259 259
Size: 4856
PsiI TTA'TAA
Cuts at: 542 542
Size: 4856
PstI C_TGCA'G
Cuts at: 362 368 389 836 1903 2588 362
Size: 6 21 447 1067 685 2630
Fragments arranged by size:
2630 1067 685 447 21 6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4856
PvuII CAG'CTG
Cuts at: 363 1258 2281 2641 363
Size: 895 1023 360 2578
Fragments arranged by size:
2578 1023 895 360
RsrII CG'GwC_CG
Cuts at: 3051 3051
Size: 4856
SacI G_AGCT'C
Cuts at: 1289 1870 1289
Size: 581 4275
SacII CC_GC'GG
Cuts at: 142 307 142
Size: 165 4691
SalI G'TCGA_C
Cuts at: 1893 1893
Size: 4856
SanDI GG'GwC_CC
Cuts at: 259 259
Size: 4856
SapI GCTCTTCn'nnn_
Cuts at: 2879 3089 2879
Size: 210 4646
SbfI CC_TGCA'GG
Cuts at: 1903 1903
Size: 4856
ScaI AGT'ACT
Cuts at: 4373 4373
Size: 4856
SexAI A'CCwGG_T
Cuts at: 1523 1523
Size: 4856
SfcI C'TryA_G
Cuts at: 33 358 364 385 636 832 1899 2584 3577
Size: 325 6 21 251 196 1067 685 993
Cuts at: 3577 3768 33
Size: 191 1121
Fragments arranged by size:
1121 1067 993 685 325 251 196 191
21 6
SfoI GGC'GCC
Cuts at: 2288 2536 2288
Size: 248 4608
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4856
SgrAI Cr'CCGG_yG
Cuts at: 312 312
Size: 4856
SmaI CCC'GGG
Cuts at: 282 1878 282
Size: 1596 3260
SmlI C'TyrA_G
Cuts at: 586 2011 2926 3418 3680 586
Size: 1425 915 492 262 1762
Fragments arranged by size:
1762 1425 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4856
SphI G_CATG'C
Cuts at: 1909 2409 1909
Size: 500 4356
SspI AAT'ATT
Cuts at: 1069 1069
Size: 4856
StuI AGG'CCT
Cuts at: 1434 1434
Size: 4856
StyI C'CwwG_G
Cuts at: 692 1990 2344 692
Size: 1298 354 3204
Fragments arranged by size:
3204 1298 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1190 1621 2439 2572 3031 3240 1190
Size: 431 818 133 459 209 2806
Fragments arranged by size:
2806 818 459 431 209 133
TatI w'GTAC_w
Cuts at: 1574 4371 1574
Size: 2797 2059
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1165 1197 1720 1752 4373 4405 1165
Size: 32 523 32 2621 32 1616
Fragments arranged by size:
2621 1616 523 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2653 2653
Size: 4856
XbaI T'CTAG_A
Cuts at: 1887 1887
Size: 4856
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2351 2351
Size: 4856
XmaI C'CCGG_G
Cuts at: 280 1876 280
Size: 1596 3260
XmnI GAAnn'nnTTC
Cuts at: 898 1828 898
Size: 930 3926
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NotI NsiI
NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PsiI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AleI AloI ApaI AscI AvrII
BaeI BbsI BglI BmtI BsiWI BsmI BsmBI BsrGI
BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NruI
PacI PasI PshAI PspOMI PspXI PsrI SfiI SnaBI
SrfI SwaI XhoI ZraI