Restriction map for pF1KB8522
##################################
# NO CUT ENZYMES (  103 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        AhdI        Alw44I      AlwNI       Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AvrII       BbvCI       
BclI        BfrI        BlnI        BlpI        Bpu1102I    
BsaI        BsaWI       BseAI       BseRI       BsiWI       
BsmBI       BsmI        BspDI       BspEI       BspHI       
BsrFI       BssSI       BstBI       CelII       Cfr10I      
ClaI        CpoI        DraI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoRI       EcoRV       
FbaI        FseI        HpaI        KpnI        KspI        
MfeI        MluI        MroI        MspA1I      MunI        
NaeI        NdeI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmeI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsrII       SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspI        SwaI        Tth111I     Van91I      XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccI         2148  GTATAC               GT^[AC][GT]AC
AocI         1696  CCTGAGG              CC^T[ACGT]AGG
Asp700I      1427  GAAAGTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         847  GAGCTC               G[AT]GC[AT]^C
AvaI         1936  CTCGGG               C^[CT]CG[GA]G
BamHI         388  GGATCC               G^GATCC
BanII         847  GAGCTC               G[GA]GC[CT]^C
BbeI          955  GGCGCC               GGCGC^C
BbrPI        2331  CACGTG               CAC^GTG
BfaI         1474  CTAG                 C^TAG
BglI         2423  GCCTGAGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         608  AGATCT               A^GATCT
BsaBI        1587  GATTTGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI         999  CGACCG               CG[GA][CT]^CG
BsiHKAI       847  GAGCTC               G[AT]GC[AT]^C
BsmAI         670  GAGAC                GTCTC(1/5)
BsoBI        1936  CTCGGG               C^[CT]CG[GA]G
BspLU11I      292  ACATGT               A^CATGT
BspMI         476  ACCTGC               ACCTGC(4/8)
BsrBI         744  CCGCTC               CCGCTC(-3/-3)
Bst1107I     2148  GTATAC               GTA^TAC
BstEII        109  GGTGACC              G^GT[ACGT]ACC
BstPI         109  GGTGACC              G^GT[ACGT]ACC
BstZ17I      2148  GTATAC               GTA^TAC
Bsu36I       1696  CCTGAGG              CC^T[ACGT]AGG
DraIII       2328  CACCACGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          630  GACCTACGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1696  CCTGAGG              CC^T[ACGT]AGG
EcoNI        1692  CCTTCCTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       109  GGTGACC              G^GT[ACGT]ACC
HindIII      1249  AAGCTT               A^AGCTT
KasI          955  GGCGCC               G^GCGCC
MaeI         1474  CTAG                 C^TAG
MamI         1587  GATTTGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI          955  GGCGCC               GG^CGCC
PmaCI        2331  CACGTG               CAC^GTG
PmlI         2331  CACGTG               CAC^GTG
PstI         1667  CTGCAG               CTGCA^G
SacI          847  GAGCTC               GAGCT^C
SapI          486  GCTCTTC              GCTCTTC(1/4)
SfoI          955  GGCGCC               GGC^GCC
StuI          683  AGGCCT               AGG^CCT
XmnI         1427  GAAAGTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   37 enzymes) #
###################################

AcsI         2041  AAATTC               [GA]^AATT[CT]
             2289  AAATTT              
AcyI          955  GGCGCC               G[GA]^CG[CT]C
             1720  GACGCC              
ApoI         2041  AAATTC               [GA]^AATT[CT]
             2289  AAATTT              
AviII         707  TGCGCA               TGC^GCA
              827  TGCGCA              
BalI          893  TGGCCA               TGG^CCA
             1130  TGGCCA              
BanI          955  GGCGCC               G^G[CT][GA]CC
             2137  GGCACC              
BbsI          184  GAAGAC               GAAGAC(2/6)
             1717  GAAGAC              
BcgI         1530  CGACCCCTGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1834  CGAGAACTCTGC        
BciVI         288  GGATAC               GTATCC(6/5)
              303  GGATAC              
BcnI         1053  CCGGG                CC^[GC]GG
             1499  CCCGG               
BpuAI         184  GAAGAC               GAAGAC(2/6)
             1717  GAAGAC              
BsaHI         955  GGCGCC               G[GA]^CG[CT]C
             1720  GACGCC              
BssHII        805  GCGCGC               G^CGCGC
              911  GCGCGC              
BstAPI        710  GCAACATCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1212  GCACAAAATGC         
DraII         125  GGGGCCT              [GA]G^G[ACGT]CC[CT]
             2129  AGGCCCC             
DsaI          599  CCATGG               C^C[GA][CT]GG
             1196  CCATGG              
EaeI          893  TGGCCA               [CT]^GGCC[GA]
             1130  TGGCCA              
EarI          487  CTCTTC               CTCTTC(1/4)
              820  CTCTTC              
EcoO109I      125  GGGGCCT              [GA]G^G[ACGT]CC[CT]
             2129  AGGCCCC             
EcoT22I       311  ATGCAT               ATGCA^T
             1773  ATGCAT              
FspI          707  TGCGCA               TGC^GCA
              827  TGCGCA              
HapII        1053  CCGG                 C^CGG
             1500  CCGG                
Hin1I         955  GGCGCC               G[GA]^CG[CT]C
             1720  GACGCC              
HincII          9  GTTGAC               GT[CT]^[GA]AC
             1480  GTCAAC              
HindII          9  GTTGAC               GT[CT]^[GA]AC
             1480  GTCAAC              
HpaII        1053  CCGG                 C^CGG
             1500  CCGG                
Ksp632I       487  CTCTTC               CTCTTC(1/4)
              820  CTCTTC              
MluNI         893  TGGCCA               TGG^CCA
             1130  TGGCCA              
MscI          893  TGGCCA               TGG^CCA
             1130  TGGCCA              
MseI           98  TTAA                 T^TAA
              140  TTAA                
MspI         1053  CCGG                 C^CGG
             1500  CCGG                
NciI         1053  CCGGG                CC^[GC]GG
             1499  CCCGG               
NcoI          599  CCATGG               C^CATGG
             1196  CCATGG              
NsiI          311  ATGCAT               ATGCA^T
             1773  ATGCAT              
SfcI         1667  CTGCAG               C^T[GA][CT]AG
             2385  CTACAG              
Tru9I          98  TTAA                 T^TAA
              140  TTAA                
XcmI         1413  CCAAAATGTAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1745  CCATTGCCTGTCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   75 enzymes) #
#########################################

AccII       AciI        AfaI        AflIII      AluI        
AlwI        AvaII       BbvI        BmyI        BpmI        
BsaAI       BsaJI       BsgI        BsiYI       BslI        
BsmFI       Bsp1286I    Bsp1407I    BsrDI       BsrGI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstXI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HgaI        
HhaI        HinP1I      HinfI       HphI        ItaI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MslI        MvaI        MvnI        MwoI        
NdeII       NlaIII      NlaIV       NspI        PleI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SspBI       StyI        TaqI        TfiI        TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII       
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