Restriction map for
pF1KE0372
(Circular) (Six-base) MAPSORT of: pF1KE0372.seq Check: 7471 from: 1 to: 4808
pF1KE0372 4808 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AatII G_ACGT'C
Cuts at: 1499 1499
Size: 4808
AccI GT'mk_AC
Cuts at: 756 1289 1323 1846 756
Size: 533 34 523 3718
Fragments arranged by size:
3718 533 523 34
Acc65I G'GTAC_C
Cuts at: 1824 1824
Size: 4808
AclI AA'CG_TT
Cuts at: 4648 4648
Size: 4808
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 1295 2633 3065 3812 4080 197
Size: 1098 1338 432 747 268 925
Fragments arranged by size:
1338 1098 925 747 432 268
AflII C'TTAA_G
Cuts at: 1500 1500
Size: 4808
AflIII A'CryG_T
Cuts at: 580 1741 2160 3264 4241 580
Size: 1161 419 1104 977 1147
Fragments arranged by size:
1161 1147 1104 977 419
AgeI A'CCGG_T
Cuts at: 3196 3196
Size: 4808
AlwNI CAG_nnn'CTG
Cuts at: 235 2113 3680 235
Size: 1878 1567 1363
Fragments arranged by size:
1878 1567 1363
ApaLI G'TGCA_C
Cuts at: 3578 3578
Size: 4808
ApoI r'AATT_y
Cuts at: 508 864 1297 1812 4161 4417 508
Size: 356 433 515 2349 256 899
Fragments arranged by size:
2349 899 515 433 356 256
AseI AT'TA_AT
Cuts at: 20 958 20
Size: 938 3870
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4808
AvaI C'yCGr_G
Cuts at: 1828 1828
Size: 4808
BamHI G'GATC_C
Cuts at: 1833 1833
Size: 4808
BanI G'GyrC_C
Cuts at: 1824 2238 2486 2521 1824
Size: 414 248 35 4111
Fragments arranged by size:
4111 414 248 35
BanII G_rGCy'C
Cuts at: 187 577 1822 2852 187
Size: 390 1245 1030 2143
Fragments arranged by size:
2143 1245 1030 390
BbeI G_GCGC'C
Cuts at: 2242 2490 2242
Size: 248 4560
BbsI GAAGACnn'nnnn_
Cuts at: 111 905 111
Size: 794 4014
BciVI GTATCCnnnnn_n'
Cuts at: 2706 3467 4808 2706
Size: 761 1341 2706
Fragments arranged by size:
2706 1341 761
BclI T'GATC_A
Cuts at: 621 962 2328 621
Size: 341 1366 3101
Fragments arranged by size:
3101 1366 341
BglII A'GATC_T
Cuts at: 660 2323 4360 660
Size: 1663 2037 1108
Fragments arranged by size:
2037 1663 1108
BlpI GC'TnA_GC
Cuts at: 1920 1920
Size: 4808
Bme1580I G_kGCm'C
Cuts at: 408 2433 2526 3582 408
Size: 2025 93 1056 1634
Fragments arranged by size:
2025 1634 1056 93
BmgBI CAC'GTC
Cuts at: 1702 1702
Size: 4808
BmrI ACTGGGnnnn_n'
Cuts at: 2020 2101 2436 4580 2020
Size: 81 335 2144 2248
Fragments arranged by size:
2248 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 281 1265 3175 281
Size: 984 1910 1914
Fragments arranged by size:
1914 1910 984
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 506 1085 1238 506
Size: 579 153 4076
Fragments arranged by size:
4076 579 153
Bpu10I CC'TnA_GC
Cuts at: 1240 1240
Size: 4808
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 763 1984 2863 3355 3653 763
Size: 1221 879 492 298 1918
Fragments arranged by size:
1918 1221 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 483 4512 483
Size: 4029 779
BsaAI yAC'GTr
Cuts at: 583 2161 583
Size: 1578 3230
BsaBI GATnn'nnATC
Cuts at: 6 2322 4359 6
Size: 2316 2037 455
Fragments arranged by size:
2316 2037 455
BsaHI Gr'CG_yC
Cuts at: 1496 2239 2487 3189 4685 1496
Size: 743 248 702 1496 1619
Fragments arranged by size:
1619 1496 743 702 248
BsaWI w'CCGG_w
Cuts at: 1997 2518 3196 3470 3617 4028 1997
Size: 521 678 274 147 411 2777
Fragments arranged by size:
2777 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1214 1244 1328 1358 1214
Size: 30 84 30 4664
Fragments arranged by size:
4664 84 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 613 788 1241 613
Size: 175 453 4180
Fragments arranged by size:
4180 453 175
BseYI C'CCAG_C
Cuts at: 2233 2991 3568 4064 2233
Size: 758 577 496 2977
Fragments arranged by size:
2977 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2350 2396 3604 104
Size: 2246 46 1208 1308
Fragments arranged by size:
2246 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 187 1822 2600 2790 3582 187
Size: 1635 778 190 792 1413
Fragments arranged by size:
1635 1413 792 778 190
BsmI GAATG_Cn'
Cuts at: 1285 1285
Size: 4808
Bsp1286I G_dGCh'C
Cuts at: 187 408 577 1822 2433 2526 2600 2790 2852
Size: 221 169 1245 611 93 74 190 62
Cuts at: 2852 3582 187
Size: 730 1413
Fragments arranged by size:
1413 1245 730 611 221 190 169 93
74 62
BspEI T'CCGG_A
Cuts at: 4028 4028
Size: 4808
BspHI T'CATG_A
Cuts at: 3984 3984
Size: 4808
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1858 2072 2374 2755 1858
Size: 214 302 381 3911
Fragments arranged by size:
3911 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3100 3154 4638 4803 50
Size: 3050 54 1484 165 55
Fragments arranged by size:
3050 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 206 1190 2720 206
Size: 984 1530 2294
Fragments arranged by size:
2294 1530 984
BsrFI r'CCGG_y
Cuts at: 2806 2987 3196 2806
Size: 181 209 4418
Fragments arranged by size:
4418 209 181
BssSI C'ACGA_G
Cuts at: 3079 3437 3079
Size: 358 4450
BstAPI GCAn_nnn'nTGC
Cuts at: 997 2185 997
Size: 1188 3620
BstBI TT'CG_AA
Cuts at: 3169 3169
Size: 4808
BstYI r'GATC_y
Cuts at: 1 660 1833 2300 2323 2658 2904 3916 4002
Size: 659 1173 467 23 335 246 1012 86
Cuts at: 4002 4014 4360 1
Size: 12 346 449
Fragments arranged by size:
1173 1012 659 467 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 1324 1324
Size: 4808
Bsu36I CC'TnA_GG
Cuts at: 1218 1218
Size: 4808
BtgI C'CryG_G
Cuts at: 107 107
Size: 4808
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2758 2939 3102 2758
Size: 181 163 4464
Fragments arranged by size:
4464 181 163
BtsI GCAGTG_nn'
Cuts at: 1402 2051 1402
Size: 649 4159
DraI TTT'AAA
Cuts at: 1807 1807
Size: 4808
DrdI GACnn_nn'nnGTC
Cuts at: 1495 2514 3372 1495
Size: 1019 858 2931
Fragments arranged by size:
2931 1019 858
EaeI y'GGCC_r
Cuts at: 852 1383 1632 2393 2567 2958 2985 4330 852
Size: 531 249 761 174 391 27 1345 1330
Fragments arranged by size:
1345 1330 761 531 391 249 174 27
EagI C'GGCC_G
Cuts at: 2393 2393
Size: 4808
EarI CTCTTCn'nnn_
Cuts at: 1082 2831 3041 1082
Size: 1749 210 2849
Fragments arranged by size:
2849 1749 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3016 3326 3472 4618 3016
Size: 310 146 1146 3206
Fragments arranged by size:
3206 1146 310 146
EcoICRI GAG'CTC
Cuts at: 185 1820 185
Size: 1635 3173
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 506 1085 1238 1295 2633 3065 3812 4080
Size: 309 579 153 57 1338 432 747 268
Cuts at: 4080 197
Size: 925
Fragments arranged by size:
1338 925 747 579 432 309 268 153
57
EcoO109I rG'GnC_Cy
Cuts at: 1947 1947
Size: 4808
EcoRI G'AATT_C
Cuts at: 1812 1812
Size: 4808
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 486 1025 2827 486
Size: 539 1802 2467
Fragments arranged by size:
2467 1802 539
FspI TGC'GCA
Cuts at: 2589 2589
Size: 4808
HaeII r_GCGC'y
Cuts at: 2242 2490 3512 4491 2242
Size: 248 1022 979 2559
Fragments arranged by size:
2559 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 970 1002 1691 1723 2650 2680 2682 2712 970
Size: 32 689 32 927 30 2 30 3066
Fragments arranged by size:
3066 927 689 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1749 1847 1749
Size: 98 4710
HindIII A'AGCT_T
Cuts at: 1157 1157
Size: 4808
HpaI GTT'AAC
Cuts at: 1749 1749
Size: 4808
KasI G'GCGC_C
Cuts at: 2238 2486 2238
Size: 248 4560
KpnI G_GTAC'C
Cuts at: 1828 1828
Size: 4808
MfeI C'AATT_G
Cuts at: 395 1403 395
Size: 1008 3800
MluI A'CGCG_T
Cuts at: 4241 4241
Size: 4808
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 396 1650 3479 3663 396
Size: 1254 1829 184 1541
Fragments arranged by size:
1829 1541 1254 184
MscI TGG'CCA
Cuts at: 854 1385 2569 854
Size: 531 1184 3093
Fragments arranged by size:
3093 1184 531
MslI CAynn'nnrTG
Cuts at: 472 559 1055 1096 1546 2797 2924 472
Size: 87 496 41 450 1251 127 2356
Fragments arranged by size:
2356 1251 496 450 127 87 41
MspA1I CmG'CkG
Cuts at: 484 1919 2233 2593 3606 3851 484
Size: 1435 314 360 1013 245 1441
Fragments arranged by size:
1441 1435 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2989 2989
Size: 4808
NarI GG'CG_CC
Cuts at: 2239 2487 2239
Size: 248 4560
NcoI C'CATG_G
Cuts at: 107 107
Size: 4808
NgoMIV G'CCGG_C
Cuts at: 2987 2987
Size: 4808
NspI r_CATG'y
Cuts at: 1745 1861 2361 3268 1745
Size: 116 500 907 3285
Fragments arranged by size:
3285 907 500 116
PciI A'CATG_T
Cuts at: 1741 3264 1741
Size: 1523 3285
PflMI CCAn_nnn'nTGG
Cuts at: 1608 1608
Size: 4808
PfoI T'CCnGG_A
Cuts at: 280 280
Size: 4808
PmeI GTTT'AAAC
Cuts at: 1807 1807
Size: 4808
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3947 3979 3947
Size: 32 4776
PsiI TTA'TAA
Cuts at: 1165 1316 1165
Size: 151 4657
PstI C_TGCA'G
Cuts at: 993 1855 2540 993
Size: 862 685 3261
Fragments arranged by size:
3261 862 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4808
PvuII CAG'CTG
Cuts at: 484 2233 2593 484
Size: 1749 360 2699
Fragments arranged by size:
2699 1749 360
RsrII CG'GwC_CG
Cuts at: 3003 3003
Size: 4808
SacI G_AGCT'C
Cuts at: 187 1822 187
Size: 1635 3173
SalI G'TCGA_C
Cuts at: 1845 1845
Size: 4808
SapI GCTCTTCn'nnn_
Cuts at: 2831 3041 2831
Size: 210 4598
SbfI CC_TGCA'GG
Cuts at: 1855 1855
Size: 4808
ScaI AGT'ACT
Cuts at: 4325 4325
Size: 4808
SfcI C'TryA_G
Cuts at: 33 753 989 1851 2536 3529 3720 33
Size: 720 236 862 685 993 191 1121
Fragments arranged by size:
1121 993 862 720 685 236 191
SfoI GGC'GCC
Cuts at: 2240 2488 2240
Size: 248 4560
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4808
SmaI CCC'GGG
Cuts at: 1830 1830
Size: 4808
SmlI C'TyrA_G
Cuts at: 742 1500 1963 2878 3370 3632 742
Size: 758 463 915 492 262 1918
Fragments arranged by size:
1918 915 758 492 463 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4808
SphI G_CATG'C
Cuts at: 1861 2361 1861
Size: 500 4308
SspI AAT'ATT
Cuts at: 783 1087 783
Size: 304 4504
StyI C'CwwG_G
Cuts at: 107 176 268 1942 2296 107
Size: 69 92 1674 354 2619
Fragments arranged by size:
2619 1674 354 92 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 693 2391 2524 2983 3192 693
Size: 1698 133 459 209 2309
Fragments arranged by size:
2309 1698 459 209 133
TatI w'GTAC_w
Cuts at: 4323 4323
Size: 4808
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4325 4357 4325
Size: 32 4776
Tth111I GACn'n_nGTC
Cuts at: 1411 2605 1411
Size: 1194 3614
XbaI T'CTAG_A
Cuts at: 1839 1839
Size: 4808
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 288 379 1605 2303 288
Size: 91 1226 698 2793
Fragments arranged by size:
2793 1226 698 91
XmaI C'CCGG_G
Cuts at: 1828 1828
Size: 4808
ZraI GAC'GTC
Cuts at: 1497 1497
Size: 4808
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmtI BsgI
BsiWI BsmBI BsrGI BssHII BstEII BstXI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PpuMI PshAI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI XmnI