Restriction map for pF1KE0979
##################################
# NO CUT ENZYMES (  109 enzymes) #
##################################

AatII       AccII       AccIII      AciI        AclI        
AflII       Alw44I      ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     AspI        AvaI        
AviII       BbrPI       BbvCI       BcgI        BciVI       
BcnI        BfrI        BsaAI       BsaI        BseAI       
BseRI       BsgI        BsiEI       BsiWI       BsmBI       
BsmFI       BsoBI       Bsp1407I    BspDI       BspEI       
BsrBI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     ClaI        CpoI        
DrdI        DsaI        EagI        EclXI       Eco52I      
EcoO65I     EcoT22I     FauI        FseI        FspI        
KspI        MaeII       MluI        MroI        MvnI        
NaeI        NciI        NcoI        NdeI        NgoMIV      
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SspBI       
SwaI        TaqI        Tth111I     TthHB8I     XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   78 enzymes) #
###################################

Acc65I       1611  GGTACC               G^GTACC
AccI          583  GTAGAC               GT^[AC][GT]AC
AflIII       1187  ACATGT               A^C[GA][CT]GT
AgeI           59  ACCGGT               A^CCGGT
AhdI         1186  GACATGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         326  CAGTGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          727  CCTAAGG              CC^T[ACGT]AGG
Aor51HI       848  AGCGCT               AGC^GCT
Asp718I      1611  GGTACC               G^GTACC
AspEI        1186  GACATGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI        1139  GAGCAC               G[AT]GC[AT]^C
AvaII         244  GGACC                G^G[AT]CC
AvrII        1114  CCTAGG               C^CTAGG
BalI          221  TGGCCA               TGG^CCA
BamHI        1294  GGATCC               G^GATCC
BanII         903  GAGCCC               G[GA]GC[CT]^C
BbeI          560  GGCGCC               GGCGC^C
BbsI         1473  GAAGAC               GAAGAC(2/6)
BclI          381  TGATCA               T^GATCA
BglI         1111  GCCCCTAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1650  AGATCT               A^GATCT
BlnI         1114  CCTAGG               C^CTAGG
BlpI          652  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      652  GCTCAGC              GC^T[ACGT]AGC
BpuAI        1473  GAAGAC               GAAGAC(2/6)
BsaBI        1651  GATCTAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI          59  ACCGGT               [AT]^CCGG[AT]
BsiHKAI      1139  GAGCAC               G[AT]GC[AT]^C
BsmAI        1389  GAGAC                GTCTC(1/5)
BsmI          500  GCATTC               GAATGC(1/-1)
BspHI         920  TCATGA               T^CATGA
BspLU11I     1187  ACATGT               A^CATGT
BsrFI          59  ACCGGT               [GA]^CCGG[CT]
Bsu36I        727  CCTAAGG              CC^T[ACGT]AGG
CelII         652  GCTCAGC              GC^T[ACGT]AGC
Cfr10I         59  ACCGGT               [GA]^CCGG[CT]
DraII         243  AGGACCT              [GA]G^G[ACGT]CC[CT]
DraIII        710  CACTGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          221  TGGCCA               [CT]^GGCC[GA]
Eam1105I     1186  GACATGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          250  GAAGAG               CTCTTC(1/4)
Eco47III      848  AGCGCT               AGC^GCT
Eco81I        727  CCTAAGG              CC^T[ACGT]AGG
EcoNI        1114  CCTAGGGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      243  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRV        1232  GATATC               GAT^ATC
FbaI          381  TGATCA               T^GATCA
HapII          60  CCGG                 C^CGG
HincII       1422  GTTAAC               GT[CT]^[GA]AC
HindII       1422  GTTAAC               GT[CT]^[GA]AC
HpaI         1422  GTTAAC               GTT^AAC
HpaII          60  CCGG                 C^CGG
HphI          522  TCACC                GGTGA(8/7)
KasI          560  GGCGCC               G^GCGCC
KpnI         1611  GGTACC               GGTAC^C
Ksp632I       250  GAAGAG               CTCTTC(1/4)
MamI         1651  GATCTAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1181  CAATTG               C^AATTG
MluNI         221  TGGCCA               TGG^CCA
MscI          221  TGGCCA               TGG^CCA
MslI          523  CACCGTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        305  CAGCTG               C[AC]G^C[GT]G
MspI           60  CCGG                 C^CGG
MunI         1181  CAATTG               C^AATTG
NarI          560  GGCGCC               GG^CGCC
NheI         1039  GCTAGC               G^CTAGC
PflMI         709  CCACTGAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI          59  ACCGGT               A^CCGGT
PpuMI         243  AGGACCT              [GA]G^G[AT]CC[CT]
PvuII         305  CAGCTG               CAG^CTG
RcaI          920  TCATGA               T^CATGA
SfaNI        1695  GCATC                GCATC(5/9)
SfoI          560  GGCGCC               GGC^GCC
SmlI         1371  CTTGAG               C^T[CT][GA]AG
SphI         1443  GCATGC               GCATG^C
SspI         1338  AATATT               AAT^ATT
StuI          834  AGGCCT               AGG^CCT
Van91I        709  CCACTGAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

BanI          560  GGCGCC               G^G[CT][GA]CC
             1611  GGTACC              
BbvI          304  GCAGC                GCAGC(8/12)
             1315  GCTGC               
BpmI         1511  CTCCAG               CTGGAG(16/14)
              409  CTGGAG              
BssKI        1129  CCAGG                ^CC[ACGT]GG
             1513  CCAGG               
BstNI        1129  CCAGG                CC^[AT]GG
             1513  CCAGG               
Cac8I        1039  GCTAGC               GC[ACGT]^[ACGT]GC
             1443  GCATGC              
CfoI          561  GCGC                 GCG^C
              849  GCGC                
EcoRI         438  GAATTC               G^AATTC
             1530  GAATTC              
EcoRII       1129  CCAGG                ^CC[AT]GG
             1513  CCAGG               
Fnu4HI        304  GCAGC                GC^[ACGT]GC
             1315  GCTGC               
HaeII         560  GGCGCC               [GA]GCGC^[CT]
              848  AGCGCT              
HgaI            7  GCGTC                GACGC(5/10)
             1098  GCGTC               
HhaI          561  GCGC                 GCG^C
              849  GCGC                
HinP1I        561  GCGC                 G^CGC
              849  GCGC                
ItaI          304  GCAGC                GC^[ACGT]GC
             1315  GCTGC               
MvaI         1129  CCAGG                CC^[AT]GG
             1513  CCAGG               
NspI         1187  ACATGT               [GA]CATG^[CT]
             1443  GCATGC              
PleI         1391  GACTC                GAGTC(4/5)
              418  GAGTC               
PstI          908  CTGCAG               CTGCA^G
             1196  CTGCAG              
ScrFI        1129  CCAGG                CC^[ACGT]GG
             1513  CCAGG               
TseI          304  GCAGC                G^C[AT]GC
             1315  GCTGC               
Tsp45I         56  GTGAC                ^GT[GC]AC
             1052  GTGAC               
XbaI          952  TCTAGA               T^CTAGA
             1647  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AcsI        AcyI        AfaI        AluI        AlwI        
ApoI        BfaI        BmyI        BsaHI       BsaJI       
BsiYI       BslI        Bsp1286I    BspMI       BsrI        
BstF5I      BstYI       Cfr13I      DdeI        DpnII       
DraI        EcoT14I     FokI        HaeIII      Hin1I       
HindIII     HinfI       MaeI        MaeIII      MboI        
MboII       MflI        MnlI        MseI        MwoI        
NdeII       NlaIII      NlaIV       RsaI        Sau3AI      
Sau96I      SfcI        StyI        TfiI        Tru9I       
Tsp509I     TspRI       XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com