Restriction map for pF1KE1947
##################################
# NO CUT ENZYMES (   99 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AflII       
AflIII      AgeI        AhdI        ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AvrII       BamHI       BbrPI       BbvCI       BcgI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaBI       BsgI        BsiWI       BsmBI       BsmI        
BspDI       BspLU11I    BsrBI       BsrFI       BssHII      
BssSI       BstBI       BstXI       CelII       Cfr10I      
ClaI        CpoI        DraI        DraIII      Eam1105I    
EcoRI       EcoRV       EcoT22I     FseI        HindIII     
HpaI        KpnI        KspI        MamI        MfeI        
MluI        MseI        MunI        NaeI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacI        
SacII       SalI        SbfI        ScaI        SfiI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspI        SwaI        Tru9I       
Tth111I     XbaI        XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   81 enzymes) #
###################################

AccI          750  GTATAC               GT^[AC][GT]AC
AccII          39  CGCG                 CG^CG
AccIII        371  TCCGGA               T^CCGGA
AcyI          106  GGCGCC               G[GA]^CG[CT]C
Alw44I       1264  GTGCAC               G^TGCAC
AlwI          816  GGATC                GGATC(4/5)
AocI          198  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      1252  AGCGCT               AGC^GCT
ApaI         1223  GGGCCC               GGGCC^C
ApaLI        1264  GTGCAC               G^TGCAC
Asp700I      1014  GAACATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI        1264  GTGCAC               G[AT]GC[AT]^C
AvaII         366  GGACC                G^G[AT]CC
AviII          89  TGCGCA               TGC^GCA
BalI         1118  TGGCCA               TGG^CCA
BbeI          106  GGCGCC               GGCGC^C
BbsI         1321  GAAGAC               GAAGAC(2/6)
BciVI         276  GGATAC               GTATCC(6/5)
BfaI          970  CTAG                 C^TAG
BpmI          580  CTGGAG               CTGGAG(16/14)
BpuAI        1321  GAAGAC               GAAGAC(2/6)
BsaAI         226  TACGTG               [CT]AC^GT[GA]
BsaHI         106  GGCGCC               G[GA]^CG[CT]C
BsaI          136  GAGACC               GGTCTC(1/5)
BsaWI         371  TCCGGA               [AT]^CCGG[AT]
BseAI         371  TCCGGA               T^CCGGA
BsiEI         162  CGGCCG               CG[GA][CT]^CG
BsiHKAI      1264  GTGCAC               G[AT]GC[AT]^C
Bsp1407I      731  TGTACA               T^GTACA
BspEI         371  TCCGGA               T^CCGGA
BspHI        1364  TCATGA               T^CATGA
BspMI        1097  ACCTGC               ACCTGC(4/8)
BsrDI         477  CATTGC               GCAATG(2/0)
BsrGI         731  TGTACA               T^GTACA
Bst1107I      750  GTATAC               GTA^TAC
BstEII       1053  GGTTACC              G^GT[ACGT]ACC
BstPI        1053  GGTTACC              G^GT[ACGT]ACC
BstUI          39  CGCG                 CG^CG
BstYI         816  GGATCT               [GA]^GATC[CT]
BstZ17I       750  GTATAC               GTA^TAC
Bsu36I        198  CCTGAGG              CC^T[ACGT]AGG
DrdI         1347  GACAGATGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          162  CGGCCG               C^GGCCG
EclXI         162  CGGCCG               C^GGCCG
Eco47III     1252  AGCGCT               AGC^GCT
Eco52I        162  CGGCCG               C^GGCCG
Eco81I        198  CCTGAGG              CC^T[ACGT]AGG
EcoNI         369  CCTCCGGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1053  GGTTACC              G^GT[ACGT]ACC
FspI           89  TGCGCA               TGC^GCA
HgaI          258  GACGC                GACGC(5/10)
Hin1I         106  GGCGCC               G[GA]^CG[CT]C
HincII       1356  GTCAAC               GT[CT]^[GA]AC
HindII       1356  GTCAAC               GT[CT]^[GA]AC
KasI          106  GGCGCC               G^GCGCC
MaeI          970  CTAG                 C^TAG
MflI          816  GGATCT               [GA]^GATC[CT]
MluNI        1118  TGGCCA               TGG^CCA
MroI          371  TCCGGA               T^CCGGA
MscI         1118  TGGCCA               TGG^CCA
MvnI           39  CGCG                 CG^CG
NarI          106  GGCGCC               GG^CGCC
NcoI          447  CCATGG               C^CATGG
PflMI        1004  CCACCCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         365  GGGACCT              [GA]G^G[AT]CC[CT]
RcaI         1364  TCATGA               T^CATGA
SapI          145  GAAGAGC              GCTCTTC(1/4)
SexAI         431  ACCTGGT              A^CC[AT]GGT
SfoI          106  GGCGCC               GGC^GCC
SmaI          131  CCCGGG               CCC^GGG
SspBI         731  TGTACA               T^GTACA
StuI          565  AGGCCT               AGG^CCT
TaqI          719  TCGA                 T^CGA
TfiI          493  GAATC                G^A[AT]TC
Tsp509I      1236  AATT                 ^AATT
TthHB8I       719  TCGA                 T^CGA
Van91I       1004  CCACCCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         816  GGATCT               [GA]^GATC[CT]
XmaCI         131  CCCGGG               C^CCGGG
XmaI          131  CCCGGG               C^CCGGG
XmnI         1014  GAACATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AfaI          732  GTAC                 GT^AC
              840  GTAC                
AlwNI         741  CAGTCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
              814  CAGGATCTG           
AvaI          131  CCCGGG               C^[CT]CG[GA]G
              151  CCCGAG              
BclI          704  TGATCA               T^GATCA
              785  TGATCA              
BglI           98  GCCTGGGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              164  GCCGGAGGGGC         
BseRI         936  CTCCTC               GAGGAG(10/8)
              154  GAGGAG              
BsmAI         136  GAGAC                GTCTC(1/5)
              743  GTCTC               
BsmFI         365  GGGAC                GGGAC(10/14)
             1125  GTCCC               
BsoBI         131  CCCGGG               C^[CT]CG[GA]G
              151  CCCGAG              
BstAPI        298  GCAGTGGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1092  GCAATACCTGC         
DdeI          199  CTGAG                C^T[ACGT]AG
              554  CTGAG               
DraII         365  GGGACCT              [GA]G^G[ACGT]CC[CT]
             1296  AGGCCCT             
EcoO109I      365  GGGACCT              [GA]G^G[ACGT]CC[CT]
             1296  AGGCCCT             
FbaI          704  TGATCA               T^GATCA
              785  TGATCA              
MslI          267  CATCCTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1173  CACTGTTATG          
PstI          637  CTGCAG               CTGCA^G
              911  CTGCAG              
PvuII         346  CAGCTG               CAG^CTG
              979  CAGCTG              
RsaI          732  GTAC                 GT^AC
              840  GTAC                
SfcI          637  CTGCAG               C^T[GA][CT]AG
              911  CTGCAG              
Tsp45I        460  GTCAC                ^GT[GC]AC
             1306  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   58 enzymes) #
#########################################

AciI        AluI        BanI        BanII       BbvI        
BcnI        BmyI        BsaJI       BsiYI       BslI        
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DpnII       DsaI        
EaeI        EarI        EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MnlI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        Sau3AI      Sau96I      ScrFI       SfaNI       
StyI        TseI        TspRI       
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