Restriction map for pF1KE1565
##################################
# NO CUT ENZYMES (  108 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AflII       
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        Asp718I     AspEI       
AspI        AviII       AvrII       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsiEI       BsiWI       Bsp1407I    BspDI       BsrBI       
BsrGI       BssHII      Bst1107I    BstAPI      BstEII      
BstPI       BstUI       BstZ17I     Bsu36I      CelII       
CfoI        ClaI        CpoI        DraIII      EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRV       EcoT22I     FbaI        FseI        
FspI        HaeII       HhaI        HinP1I      HindIII     
HpaI        KasI        KpnI        KspI        MfeI        
MluI        MunI        MvnI        NarI        NcoI        
NheI        NotI        NruI        NsiI        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PstI        PvuI        RsrII       SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SgrAI       SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AccIII       1619  TCCGGA               T^CCGGA
AclI         1552  AACGTT               AA^CGTT
AseI         1389  ATTAAT               AT^TAAT
AsnI         1389  ATTAAT               AT^TAAT
Asp700I      2034  GAAGATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          110  CCCGGG               C^[CT]CG[GA]G
BamHI         832  GGATCC               G^GATCC
BanI          105  GGTGCC               G^G[CT][GA]CC
BbsI          444  GTCTTC               GAAGAC(2/6)
BfaI         1483  CTAG                 C^TAG
BpuAI         444  GTCTTC               GAAGAC(2/6)
BsaAI         540  CACGTA               [CT]AC^GT[GA]
BsaBI        1896  GATCATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        1619  TCCGGA               T^CCGGA
BseRI        1412  GAGGAG               GAGGAG(10/8)
BsmBI        1714  GAGACG               CGTCTC(1/5)
BsmI          801  GAATGC               GAATGC(1/-1)
BsoBI         110  CCCGGG               C^[CT]CG[GA]G
BspEI        1619  TCCGGA               T^CCGGA
BspLU11I     2148  ACATGT               A^CATGT
BsrDI         793  GCAATG               GCAATG(2/0)
BssSI         144  CACGAG               CACGAG(-5/-1)
BstBI         512  TTCGAA               TT^CGAA
BstXI         868  CCATCGGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI         1968  TTTAAA               TTT^AAA
DraII        1688  GGGTCCC              [GA]G^G[ACGT]CC[CT]
DrdI          814  GACGAAGGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         1911  CCGTGG               C^C[GA][CT]GG
EcoNI         156  CCTGTTCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     1688  GGGTCCC              [GA]G^G[ACGT]CC[CT]
FauI         1234  CCCGC                CCCGC(4/6)
MaeI         1483  CTAG                 C^TAG
MamI         1896  GATCATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         1619  TCCGGA               T^CCGGA
NdeI          275  CATATG               CA^TATG
NspV          512  TTCGAA               TT^CGAA
PflMI         668  CCAGAAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1688  GGGTCCC              [GA]G^G[AT]CC[CT]
PshAI         262  GACTTCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        1389  ATTAAT               AT^TAAT
Psp1406I     1552  AACGTT               AA^CGTT
PvuII        1792  CAGCTG               CAG^CTG
SacI          912  GAGCTC               GAGCT^C
SapI           35  GAAGAGC              GCTCTTC(1/4)
SfcI          362  CTATAG               C^T[GA][CT]AG
SfuI          512  TTCGAA               TT^CGAA
SmaI          110  CCCGGG               CCC^GGG
StuI         1147  AGGCCT               AGG^CCT
Van91I        668  CCAGAAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         110  CCCGGG               C^CCGGG
XmaI          110  CCCGGG               C^CCGGG
XmnI         2034  GAAGATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AcyI          115  GGCGTC               G[GA]^CG[CT]C
              859  GGCGTC              
AflIII        539  ACACGT               A^C[GA][CT]GT
             2148  ACATGT              
AlwNI         669  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1792  CAGCTGCTG           
AspHI         734  GAGCAC               G[AT]GC[AT]^C
              912  GAGCTC              
BalI          936  TGGCCA               TGG^CCA
             1859  TGGCCA              
BanII         912  GAGCTC               G[GA]GC[CT]^C
             1735  GAGCCC              
BbvI           64  GCAGC                GCAGC(8/12)
             1794  GCTGC               
BsaHI         115  GGCGTC               G[GA]^CG[CT]C
              859  GGCGTC              
BsaI         1843  GAGACC               GGTCTC(1/5)
             2097  GGTCTC              
BsiHKAI       734  GAGCAC               G[AT]GC[AT]^C
              912  GAGCTC              
BspHI         407  TCATGA               T^CATGA
             1535  TCATGA              
BspMI          77  ACCTGC               ACCTGC(4/8)
              122  ACCTGC              
BsrFI        1774  GCCGGC               [GA]^CCGG[CT]
             2087  GCCGGC              
BstYI         291  GGATCT               [GA]^GATC[CT]
              832  GGATCC              
Cfr10I       1774  GCCGGC               [GA]^CCGG[CT]
             2087  GCCGGC              
EcoRI        1083  GAATTC               G^AATTC
             1702  GAATTC              
Hin1I         115  GGCGTC               G[GA]^CG[CT]C
              859  GGCGTC              
MflI          291  GGATCT               [GA]^GATC[CT]
              832  GGATCC              
MluNI         936  TGGCCA               TGG^CCA
             1859  TGGCCA              
MscI          936  TGGCCA               TGG^CCA
             1859  TGGCCA              
MspA1I         65  CAGCGG               C[AC]G^C[GT]G
             1792  CAGCTG              
NaeI         1774  GCCGGC               GCC^GGC
             2087  GCCGGC              
NgoMIV       1774  GCCGGC               G^CCGGC
             2087  GCCGGC              
PleI          495  GACTC                GAGTC(4/5)
             2137  GACTC               
RcaI          407  TCATGA               T^CATGA
             1535  TCATGA              
TseI           64  GCAGC                G^C[AT]GC
             1794  GCTGC               
XhoII         291  GGATCT               [GA]^GATC[CT]
              832  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   71 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AvaII       BcnI        BmyI        BpmI        
BsaJI       BsaWI       BsgI        BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Cac8I       Cfr13I      DdeI        
DpnII       EaeI        EarI        EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HincII      HindII      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MnlI        MseI        MslI        MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       StyI        TaqI        TfiI        
Tru9I       Tsp45I      Tsp509I     TspRI       TthHB8I     
XcmI        
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