Restriction map for
pF1KE0331
(Circular) (Six-base) MAPSORT of: pF1KE0331.seq Check: 4199 from: 1 to: 5075
pF1KE0331 5075 bp
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 1775 2113 1775
Size: 338 4737
Acc65I G'GTAC_C
Cuts at: 2091 2091
Size: 5075
AclI AA'CG_TT
Cuts at: 4915 4915
Size: 5075
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 680 743 830 1121 2900 3332 4079 4347 680
Size: 63 87 291 1779 432 747 268 1408
Fragments arranged by size:
1779 1408 747 432 291 268 87 63
AflIII A'CryG_T
Cuts at: 2427 3531 4508 2427
Size: 1104 977 2994
Fragments arranged by size:
2994 1104 977
AgeI A'CCGG_T
Cuts at: 3463 3463
Size: 5075
AhdI GACnn_n'nnGTC
Cuts at: 161 161
Size: 5075
AlwNI CAG_nnn'CTG
Cuts at: 1563 1917 2380 3947 1563
Size: 354 463 1567 2691
Fragments arranged by size:
2691 1567 463 354
ApaLI G'TGCA_C
Cuts at: 3845 3845
Size: 5075
ApoI r'AATT_y
Cuts at: 115 425 1603 1706 1758 1956 2079 4428 4684
Size: 310 1178 103 52 198 123 2349 256
Cuts at: 4684 115
Size: 506
Fragments arranged by size:
2349 1178 506 310 256 198 123 103
52
AseI AT'TA_AT
Cuts at: 20 1093 20
Size: 1073 4002
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5075
AvaI C'yCGr_G
Cuts at: 2095 2095
Size: 5075
BamHI G'GATC_C
Cuts at: 2100 2100
Size: 5075
BanI G'GyrC_C
Cuts at: 2091 2505 2753 2788 2091
Size: 414 248 35 4378
Fragments arranged by size:
4378 414 248 35
BanII G_rGCy'C
Cuts at: 2089 3119 2089
Size: 1030 4045
BbeI G_GCGC'C
Cuts at: 2509 2757 2509
Size: 248 4827
BbsI GAAGACnn'nnnn_
Cuts at: 454 638 1859 454
Size: 184 1221 3670
Fragments arranged by size:
3670 1221 184
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1771 1805 1771
Size: 34 5041
BciVI GTATCCnnnnn_n'
Cuts at: 1071 2973 3734 5075 1071
Size: 1902 761 1341 1071
Fragments arranged by size:
1902 1341 1071 761
BclI T'GATC_A
Cuts at: 2595 2595
Size: 5075
BglII A'GATC_T
Cuts at: 1810 2590 4627 1810
Size: 780 2037 2258
Fragments arranged by size:
2258 2037 780
BlpI GC'TnA_GC
Cuts at: 2187 2187
Size: 5075
Bme1580I G_kGCm'C
Cuts at: 2700 2793 3849 2700
Size: 93 1056 3926
Fragments arranged by size:
3926 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 702 2287 2368 2703 4847 702
Size: 1585 81 335 2144 930
Fragments arranged by size:
2144 1585 930 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3442 3442
Size: 5075
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 791 1619 187
Size: 604 828 3643
Fragments arranged by size:
3643 828 604
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1210 1239 2251 3130 3622 3920 1210
Size: 29 1012 879 492 298 2365
Fragments arranged by size:
2365 1012 879 492 298 29
BsaI GGTCTCn'nnnn_
Cuts at: 1686 4779 1686
Size: 3093 1982
BsaAI yAC'GTr
Cuts at: 2428 2428
Size: 5075
BsaBI GATnn'nnATC
Cuts at: 6 1619 2589 4626 6
Size: 1613 970 2037 455
Fragments arranged by size:
2037 1613 970 455
BsaHI Gr'CG_yC
Cuts at: 2506 2754 3456 4952 2506
Size: 248 702 1496 2629
Fragments arranged by size:
2629 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2264 2785 3463 3737 3884 4295 2264
Size: 521 678 274 147 411 3044
Fragments arranged by size:
3044 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 812 812
Size: 5075
BseYI C'CCAG_C
Cuts at: 2500 3258 3835 4331 2500
Size: 758 577 496 3244
Fragments arranged by size:
3244 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2617 2663 3871 104
Size: 2513 46 1208 1308
Fragments arranged by size:
2513 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1007 2089 2867 3057 3849 1007
Size: 1082 778 190 792 2233
Fragments arranged by size:
2233 1082 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1007 2089 2700 2793 2867 3057 3119 3849 1007
Size: 1082 611 93 74 190 62 730 2233
Fragments arranged by size:
2233 1082 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4295 4295
Size: 5075
BspHI T'CATG_A
Cuts at: 1952 4251 1952
Size: 2299 2776
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2125 2339 2641 3022 2125
Size: 214 302 381 4178
Fragments arranged by size:
4178 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3367 3421 4905 5070 50
Size: 3317 54 1484 165 55
Fragments arranged by size:
3317 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2987 2987
Size: 5075
BsrFI r'CCGG_y
Cuts at: 3073 3254 3463 3073
Size: 181 209 4685
Fragments arranged by size:
4685 209 181
BsrGI T'GTAC_A
Cuts at: 843 1171 843
Size: 328 4747
BssSI C'ACGA_G
Cuts at: 3346 3704 3346
Size: 358 4717
BstAPI GCAn_nnn'nTGC
Cuts at: 2452 2452
Size: 5075
BstBI TT'CG_AA
Cuts at: 1762 3436 1762
Size: 1674 3401
BstYI r'GATC_y
Cuts at: 1 1810 2100 2567 2590 2925 3171 4183 4269
Size: 1809 290 467 23 335 246 1012 86
Cuts at: 4269 4281 4627 1
Size: 12 346 449
Fragments arranged by size:
1809 1012 467 449 346 335 290 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 5075
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3025 3206 3369 3025
Size: 181 163 4731
Fragments arranged by size:
4731 181 163
BtsI GCAGTG_nn'
Cuts at: 884 2318 884
Size: 1434 3641
DraI TTT'AAA
Cuts at: 2010 2074 2010
Size: 64 5011
DrdI GACnn_nn'nnGTC
Cuts at: 2781 3639 2781
Size: 858 4217
EaeI y'GGCC_r
Cuts at: 2660 2834 3225 3252 4597 2660
Size: 174 391 27 1345 3138
Fragments arranged by size:
3138 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2660 2660
Size: 5075
EarI CTCTTCn'nnn_
Cuts at: 293 398 806 3098 3308 293
Size: 105 408 2292 210 2060
Fragments arranged by size:
2292 2060 408 210 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3283 3593 3739 4885 3283
Size: 310 146 1146 3473
Fragments arranged by size:
3473 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2087 2087
Size: 5075
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 680 743 791 830 1121 1619 2900 3332
Size: 493 63 48 39 291 498 1281 432
Cuts at: 3332 4079 4347 187
Size: 747 268 915
Fragments arranged by size:
1281 915 747 498 493 432 291 268
63 48 39
EcoO109I rG'GnC_Cy
Cuts at: 262 1786 2214 262
Size: 1524 428 3123
Fragments arranged by size:
3123 1524 428
EcoRI G'AATT_C
Cuts at: 1706 1956 2079 1706
Size: 250 123 4702
Fragments arranged by size:
4702 250 123
EcoRV GAT'ATC
Cuts at: 305 666 305
Size: 361 4714
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2021 3094 2021
Size: 1073 4002
FspI TGC'GCA
Cuts at: 2856 2856
Size: 5075
HaeII r_GCGC'y
Cuts at: 2509 2757 3779 4758 2509
Size: 248 1022 979 2826
Fragments arranged by size:
2826 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 147 179 377 409 544 576 1551 1583 1666
Size: 32 198 32 135 32 975 32 83
Cuts at: 1666 1698 2917 2947 2949 2979 147
Size: 32 1219 30 2 30 2243
Fragments arranged by size:
2243 1219 975 198 135 83 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 437 2114 437
Size: 1677 3398
HindIII A'AGCT_T
Cuts at: 221 221
Size: 5075
KasI G'GCGC_C
Cuts at: 2505 2753 2505
Size: 248 4827
KpnI G_GTAC'C
Cuts at: 2095 2095
Size: 5075
MfeI C'AATT_G
Cuts at: 1973 1973
Size: 5075
MluI A'CGCG_T
Cuts at: 4508 4508
Size: 5075
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3746 3930 3746
Size: 184 4891
MscI TGG'CCA
Cuts at: 2836 2836
Size: 5075
MslI CAynn'nnrTG
Cuts at: 3064 3191 3064
Size: 127 4948
MspA1I CmG'CkG
Cuts at: 763 2186 2500 2860 3873 4118 763
Size: 1423 314 360 1013 245 1720
Fragments arranged by size:
1720 1423 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3256 3256
Size: 5075
NarI GG'CG_CC
Cuts at: 2506 2754 2506
Size: 248 4827
NcoI C'CATG_G
Cuts at: 107 107
Size: 5075
NdeI CA'TA_TG
Cuts at: 1272 1272
Size: 5075
NgoMIV G'CCGG_C
Cuts at: 3254 3254
Size: 5075
NspI r_CATG'y
Cuts at: 748 2128 2628 3535 748
Size: 1380 500 907 2288
Fragments arranged by size:
2288 1380 907 500
PciI A'CATG_T
Cuts at: 3531 3531
Size: 5075
PflMI CCAn_nnn'nTGG
Cuts at: 1031 1031
Size: 5075
PmeI GTTT'AAAC
Cuts at: 2074 2074
Size: 5075
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4214 4246 4214
Size: 32 5043
PpuMI rG'GwC_Cy
Cuts at: 262 262
Size: 5075
PstI C_TGCA'G
Cuts at: 2122 2807 2122
Size: 685 4390
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5075
PvuII CAG'CTG
Cuts at: 763 2500 2860 763
Size: 1737 360 2978
Fragments arranged by size:
2978 1737 360
RsrII CG'GwC_CG
Cuts at: 3270 3270
Size: 5075
SacI G_AGCT'C
Cuts at: 2089 2089
Size: 5075
SalI G'TCGA_C
Cuts at: 2112 2112
Size: 5075
SapI GCTCTTCn'nnn_
Cuts at: 3098 3308 3098
Size: 210 4865
SbfI CC_TGCA'GG
Cuts at: 2122 2122
Size: 5075
ScaI AGT'ACT
Cuts at: 621 4592 621
Size: 3971 1104
SfcI C'TryA_G
Cuts at: 33 2118 2803 3796 3987 33
Size: 2085 685 993 191 1121
Fragments arranged by size:
2085 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2507 2755 2507
Size: 248 4827
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5075
SmaI CCC'GGG
Cuts at: 2097 2097
Size: 5075
SmlI C'TyrA_G
Cuts at: 1218 1225 2230 3145 3637 3899 1218
Size: 7 1005 915 492 262 2394
Fragments arranged by size:
2394 1005 915 492 262 7
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5075
SphI G_CATG'C
Cuts at: 2128 2628 2128
Size: 500 4575
SspI AAT'ATT
Cuts at: 1181 1181
Size: 5075
StyI C'CwwG_G
Cuts at: 107 970 1277 1507 2209 2563 107
Size: 863 307 230 702 354 2619
Fragments arranged by size:
2619 863 702 354 307 230
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2658 2791 3250 3459 2658
Size: 133 459 209 4274
Fragments arranged by size:
4274 459 209 133
TatI w'GTAC_w
Cuts at: 172 619 843 1171 4590 172
Size: 447 224 328 3419 657
Fragments arranged by size:
3419 657 447 328 224
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 328 360 4592 4624 328
Size: 32 4232 32 779
Fragments arranged by size:
4232 779 32 32
Tth111I GACn'n_nGTC
Cuts at: 978 2872 978
Size: 1894 3181
XbaI T'CTAG_A
Cuts at: 2106 2106
Size: 5075
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1560 2570 1560
Size: 1010 4065
XmaI C'CCGG_G
Cuts at: 2095 2095
Size: 5075
XmnI GAAnn'nnTTC
Cuts at: 1483 1483
Size: 5075
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PflMI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI FseI
FspAI HpaI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI ZraI