Restriction map for
pF1KE0331
(Linear) (Six-base) MAPSORT of: KIEE0331.seq Check: 2662 from: 1 to: 1962
KIEE0331.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 0 1667 1962
Size: 1667 295
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 572 635 722 1013 1962
Size: 572 63 87 291 949
Fragments arranged by size:
949 572 291 87 63
AhdI GACnn_n'nnGTC
Cuts at: 0 53 1962
Size: 53 1909
AlwNI CAG_nnn'CTG
Cuts at: 0 1455 1809 1962
Size: 1455 354 153
Fragments arranged by size:
1455 354 153
ApoI r'AATT_y
Cuts at: 0 7 317 1495 1598 1650 1848 1962
Size: 7 310 1178 103 52 198 114
Fragments arranged by size:
1178 310 198 114 103 52 7
AseI AT'TA_AT
Cuts at: 0 985 1962
Size: 985 977
BbsI GAAGACnn'nnnn_
Cuts at: 0 346 530 1751 1962
Size: 346 184 1221 211
Fragments arranged by size:
1221 346 211 184
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1663 1697 1962
Size: 1663 34 265
Fragments arranged by size:
1663 265 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 963 1962
Size: 963 999
BglII A'GATC_T
Cuts at: 0 1702 1962
Size: 1702 260
BmrI ACTGGGnnnn_n'
Cuts at: 0 594 1962
Size: 594 1368
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 683 1511 1962
Size: 79 604 828 451
Fragments arranged by size:
828 604 451 79
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1102 1131 1962
Size: 1102 29 831
Fragments arranged by size:
1102 831 29
BsaI GGTCTCn'nnnn_
Cuts at: 0 1578 1962
Size: 1578 384
BsaBI GATnn'nnATC
Cuts at: 0 1511 1962
Size: 1511 451
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 704 1962
Size: 704 1258
BsiHKAI G_wGCw'C
Cuts at: 0 899 1962
Size: 899 1063
Bsp1286I G_dGCh'C
Cuts at: 0 899 1962
Size: 899 1063
BspHI T'CATG_A
Cuts at: 0 1844 1962
Size: 1844 118
BsrGI T'GTAC_A
Cuts at: 0 735 1063 1962
Size: 735 328 899
Fragments arranged by size:
899 735 328
BstBI TT'CG_AA
Cuts at: 0 1654 1962
Size: 1654 308
BstYI r'GATC_y
Cuts at: 0 1702 1962
Size: 1702 260
BtsI GCAGTG_nn'
Cuts at: 0 776 1962
Size: 776 1186
DraI TTT'AAA
Cuts at: 0 1902 1962
Size: 1902 60
EarI CTCTTCn'nnn_
Cuts at: 0 185 290 698 1962
Size: 185 105 408 1264
Fragments arranged by size:
1264 408 185 105
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 572 635 683 722 1013 1511 1962
Size: 79 493 63 48 39 291 498 451
Fragments arranged by size:
498 493 451 291 79 63 48 39
EcoO109I rG'GnC_Cy
Cuts at: 0 154 1678 1962
Size: 154 1524 284
Fragments arranged by size:
1524 284 154
EcoRI G'AATT_C
Cuts at: 0 1598 1848 1962
Size: 1598 250 114
Fragments arranged by size:
1598 250 114
EcoRV GAT'ATC
Cuts at: 0 197 558 1962
Size: 197 361 1404
Fragments arranged by size:
1404 361 197
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1913 1962
Size: 1913 49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 39 71 269 301 436 468 1443 1475
Size: 39 32 198 32 135 32 975 32
Cuts at: 1475 1558 1590 1962
Size: 83 32 372
Fragments arranged by size:
975 372 198 135 83 39 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 329 1962
Size: 329 1633
HindIII A'AGCT_T
Cuts at: 0 113 1962
Size: 113 1849
MfeI C'AATT_G
Cuts at: 0 1865 1962
Size: 1865 97
MspA1I CmG'CkG
Cuts at: 0 655 1962
Size: 655 1307
NdeI CA'TA_TG
Cuts at: 0 1164 1962
Size: 1164 798
NspI r_CATG'y
Cuts at: 0 640 1962
Size: 640 1322
PflMI CCAn_nnn'nTGG
Cuts at: 0 923 1962
Size: 923 1039
PpuMI rG'GwC_Cy
Cuts at: 0 154 1962
Size: 154 1808
PvuII CAG'CTG
Cuts at: 0 655 1962
Size: 655 1307
ScaI AGT'ACT
Cuts at: 0 513 1962
Size: 513 1449
SmlI C'TyrA_G
Cuts at: 0 1110 1117 1962
Size: 1110 7 845
Fragments arranged by size:
1110 845 7
SspI AAT'ATT
Cuts at: 0 1073 1962
Size: 1073 889
StyI C'CwwG_G
Cuts at: 0 862 1169 1399 1962
Size: 862 307 230 563
Fragments arranged by size:
862 563 307 230
TatI w'GTAC_w
Cuts at: 0 64 511 735 1063 1962
Size: 64 447 224 328 899
Fragments arranged by size:
899 447 328 224 64
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 220 252 1962
Size: 220 32 1710
Fragments arranged by size:
1710 220 32
Tth111I GACn'n_nGTC
Cuts at: 0 870 1962
Size: 870 1092
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1452 1962
Size: 1452 510
XmnI GAAnn'nnTTC
Cuts at: 0 1375 1962
Size: 1375 587
Enzymes that do cut:
AccI AcuI AhdI AlwNI ApoI AseI BbsI BcgI
BciVI BglII BmrI BpmI BpuEI BsaI BsaBI BseRI
BsiHKAI Bsp1286I BspHI BsrGI BstBI BstYI BtsI DraI
EarI Eco57MI EcoO109I EcoRI EcoRV FalI Hin4I HincII
HindIII MfeI MspA1I NdeI NspI PflMI PpuMI PvuII
ScaI SmlI SspI StyI TatI TstI Tth111I XcmI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbvCI BclI
BfrBI BglI BlpI Bme1580I BmgBI BmtI BplI Bpu10I
BsaAI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraIII DrdI EaeI EagI EciI EcoICRI
EcoNI FseI FspI FspAI HaeII HpaI KasI KpnI
MluI MmeI MscI MslI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI StuI SwaI TaqII XbaI
XhoI XmaI ZraI