Restriction map for pF1KE0323
##################################
# NO CUT ENZYMES (   93 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AflII       
AflIII      AgeI        Alw44I      AocI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp718I     
AviII       BbeI        BbrPI       BciVI       BclI        
BfrI        BglI        BsaAI       BsaBI       BsiEI       
BsmBI       Bsp1407I    BspDI       BspLU11I    BsrBI       
BsrGI       BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      ClaI        CpoI        
DraI        DraIII      DrdI        EagI        EclXI       
Eco52I      Eco81I      EcoO65I     EcoRI       EcoRV       
FbaI        FseI        FspI        HpaI        KasI        
KpnI        MamI        MfeI        MluI        MunI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        RsrII       SalI        SbfI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        Sse8387I    SspBI       
SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccI         1105  GTAGAC               GT^[AC][GT]AC
AccIII       1796  TCCGGA               T^CCGGA
AcyI         1599  GACGCC               G[GA]^CG[CT]C
Aor51HI       695  AGCGCT               AGC^GCT
ApaI         1307  GGGCCC               GGGCC^C
Asp700I       303  GAAGCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         2250  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        1982  GGATCC               G^GATCC
BcgI         1611  GCAGACCCCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII        1432  AGATCT               A^GATCT
BsaHI        1599  GACGCC               G[GA]^CG[CT]C
BsaWI        1796  TCCGGA               [AT]^CCGG[AT]
BseAI        1796  TCCGGA               T^CCGGA
BsgI         2428  CTGCAC               GTGCAG(16/14)
BsiWI         480  CGTACG               C^GTACG
BspEI        1796  TCCGGA               T^CCGGA
BspHI         704  TCATGA               T^CATGA
BsrDI         494  GCAATG               GCAATG(2/0)
BsrFI        1088  ACCGGC               [GA]^CCGG[CT]
BssHII       2395  GCGCGC               G^CGCGC
BstAPI        485  GCACTGTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         405  CCAGATCAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I       1088  ACCGGC               [GA]^CCGG[CT]
Eco47III      695  AGCGCT               AGC^GCT
EcoNI         276  CCTGGATGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I      1915  ATGCAT               ATGCA^T
Hin1I        1599  GACGCC               G[GA]^CG[CT]C
HincII        190  GTCAAC               GT[CT]^[GA]AC
HindII        190  GTCAAC               GT[CT]^[GA]AC
HindIII       304  AAGCTT               A^AGCTT
KspI          342  CCGCGG               CCGC^GG
MroI         1796  TCCGGA               T^CCGGA
NcoI         1628  CCATGG               C^CATGG
NsiI         1915  ATGCAT               ATGCA^T
PflFI        2250  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1842  CCAGGAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         793  GACCAAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI          704  TCATGA               T^CATGA
SacII         342  CCGCGG               CCGC^GG
ScaI          152  AGTACT               AGT^ACT
SmaI         1419  CCCGGG               CCC^GGG
SphI          828  GCATGC               GCATG^C
SspI          758  AATATT               AAT^ATT
Tth111I      2250  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1842  CCAGGAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI        1419  CCCGGG               C^CCGGG
XmaI         1419  CCCGGG               C^CCGGG
XmnI          303  GAAGCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AhdI         1108  GACCCCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1894  GACCTGCAGTC         
AlwNI         623  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2345  CAGAGTCTG           
AspEI        1108  GACCCCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1894  GACCTGCAGTC         
AvrII        2245  CCTAGG               C^CTAGG
             2370  CCTAGG              
BlnI         2245  CCTAGG               C^CTAGG
             2370  CCTAGG              
BspMI        1895  ACCTGC               ACCTGC(4/8)
             2329  ACCTGC              
Eam1105I     1108  GACCCCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1894  GACCTGCAGTC         
HaeII         695  AGCGCT               [GA]GCGC^[CT]
             1685  AGCGCC              
HphI            3  GGTGA                GGTGA(8/7)
              368  TCACC               
MaeII        1103  ACGT                 A^CGT
             2408  ACGT                
MseI         1778  TTAA                 T^TAA
             2131  TTAA                
PvuII         235  CAGCTG               CAG^CTG
             1069  CAGCTG              
SacI          499  GAGCTC               GAGCT^C
             1353  GAGCTC              
StuI          355  AGGCCT               AGG^CCT
             1659  AGGCCT              
Tru9I        1778  TTAA                 T^TAA
             2131  TTAA                
Tsp509I      1017  AATT                 ^AATT
             2174  AATT                
XcmI          405  CCAGATCAATGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1628  CCATGGAATCCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  100 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbsI        BbvCI       BbvI        BcnI        
BfaI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaI        BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
BsoBI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstUI       BstYI       Cac8I       CelII       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        HinP1I      HinfI       
HpaII       ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PleI        PpuMI       PstI        
RsaI        SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StyI        TaqI        TfiI        
TseI        Tsp45I      TspRI       TthHB8I     XhoII       
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