Restriction map for
pF1KE0313
(Circular) (Six-base) MAPSORT of: pF1KE0313.seq Check: 5615 from: 1 to: 6122
pF1KE0313 6122 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1716 1716
Size: 6122
AatII G_ACGT'C
Cuts at: 1550 1550
Size: 6122
AccI GT'mk_AC
Cuts at: 2938 3160 2938
Size: 222 5900
Acc65I G'GTAC_C
Cuts at: 1869 3138 1869
Size: 1269 4853
AclI AA'CG_TT
Cuts at: 5962 5962
Size: 6122
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 321 877 1015 1120 2519 2548 3947 4379 5126
Size: 556 138 105 1399 29 1399 432 747
Cuts at: 5126 5394 321
Size: 268 1049
Fragments arranged by size:
1399 1399 1049 747 556 432 268 138
105 29
AflII C'TTAA_G
Cuts at: 2803 2803
Size: 6122
AflIII A'CryG_T
Cuts at: 3474 4578 5555 3474
Size: 1104 977 4041
Fragments arranged by size:
4041 1104 977
AgeI A'CCGG_T
Cuts at: 4510 4510
Size: 6122
AhdI GACnn_n'nnGTC
Cuts at: 1188 1188
Size: 6122
AleI CACnn'nnGTG
Cuts at: 1708 1708
Size: 6122
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 571 2384 571
Size: 1813 4309
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 689 721 689
Size: 32 6090
AlwNI CAG_nnn'CTG
Cuts at: 1467 1786 3427 4994 1467
Size: 319 1641 1567 2595
Fragments arranged by size:
2595 1641 1567 319
ApaI G_GGCC'C
Cuts at: 500 682 2257 3032 500
Size: 182 1575 775 3590
Fragments arranged by size:
3590 1575 775 182
ApaLI G'TGCA_C
Cuts at: 1357 4892 1357
Size: 3535 2587
ApoI r'AATT_y
Cuts at: 2707 2985 3126 5475 5731 2707
Size: 278 141 2349 256 3098
Fragments arranged by size:
3098 2349 278 256 141
AseI AT'TA_AT
Cuts at: 20 20
Size: 6122
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6122
AvaI C'yCGr_G
Cuts at: 323 509 1541 2837 3142 323
Size: 186 1032 1296 305 3303
Fragments arranged by size:
3303 1296 1032 305 186
BamHI G'GATC_C
Cuts at: 3147 3147
Size: 6122
BanI G'GyrC_C
Cuts at: 1194 1536 1645 1687 1869 3138 3552 3800 3835
Size: 342 109 42 182 1269 414 248 35
Cuts at: 3835 1194
Size: 3481
Fragments arranged by size:
3481 1269 414 342 248 182 109 42
35
BanII G_rGCy'C
Cuts at: 500 682 2257 2578 3032 3116 3136 4166 500
Size: 182 1575 321 454 84 20 1030 2456
Fragments arranged by size:
2456 1575 1030 454 321 182 84 20
BbeI G_GCGC'C
Cuts at: 3556 3804 3556
Size: 248 5874
BbsI GAAGACnn'nnnn_
Cuts at: 659 659
Size: 6122
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 6088
BciVI GTATCCnnnnn_n'
Cuts at: 2197 4020 4781 6122 2197
Size: 1823 761 1341 2197
Fragments arranged by size:
2197 1823 1341 761
BclI T'GATC_A
Cuts at: 2016 3642 2016
Size: 1626 4496
BglI GCCn_nnn'nGGC
Cuts at: 2261 2261
Size: 6122
BglII A'GATC_T
Cuts at: 434 3637 5674 434
Size: 3203 2037 882
Fragments arranged by size:
3203 2037 882
BlpI GC'TnA_GC
Cuts at: 1387 3010 3234 1387
Size: 1623 224 4275
Fragments arranged by size:
4275 1623 224
Bme1580I G_kGCm'C
Cuts at: 500 682 1199 1361 1488 1648 2257 3032 3747
Size: 182 517 162 127 160 609 775 715
Cuts at: 3747 3840 4896 500
Size: 93 1056 1726
Fragments arranged by size:
1726 1056 775 715 609 517 182 162
160 127 93
BmgBI CAC'GTC
Cuts at: 360 360
Size: 6122
BmrI ACTGGGnnnn_n'
Cuts at: 107 582 627 685 1908 1998 2209 2268 3334
Size: 475 45 58 1223 90 211 59 1066
Cuts at: 3334 3415 3750 5894 107
Size: 81 335 2144 335
Fragments arranged by size:
2144 1223 1066 475 335 335 211 90
81 59 58 45
BmtI G_CTAG'C
Cuts at: 430 430
Size: 6122
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4489 4489
Size: 6122
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 982 1108 1165 1207 1264 1274 1510 1618 1667
Size: 126 57 42 57 10 236 108 49
Cuts at: 1667 2236 2932 982
Size: 569 696 4172
Fragments arranged by size:
4172 696 569 236 126 108 57 57
49 42 10
Bpu10I CC'TnA_GC
Cuts at: 947 1408 2572 947
Size: 461 1164 4497
Fragments arranged by size:
4497 1164 461
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1561 3298 4177 4669 4967 1561
Size: 1737 879 492 298 2716
Fragments arranged by size:
2716 1737 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 927 2001 2138 2312 5826 927
Size: 1074 137 174 3514 1223
Fragments arranged by size:
3514 1223 1074 174 137
BsaAI yAC'GTr
Cuts at: 3475 3475
Size: 6122
BsaBI GATnn'nnATC
Cuts at: 6 3636 5673 6
Size: 3630 2037 455
Fragments arranged by size:
3630 2037 455
BsaHI Gr'CG_yC
Cuts at: 1547 2591 3553 3801 4503 5999 1547
Size: 1044 962 248 702 1496 1670
Fragments arranged by size:
1670 1496 1044 962 702 248
BsaWI w'CCGG_w
Cuts at: 892 1589 2606 3311 3832 4510 4784 4931 5342
Size: 697 1017 705 521 678 274 147 411
Cuts at: 5342 892
Size: 1672
Fragments arranged by size:
1672 1017 705 697 678 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3085 3115 3085
Size: 30 6092
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 261 393 261
Size: 132 5990
BseYI C'CCAG_C
Cuts at: 350 390 738 762 978 1082 1849 2157 2402
Size: 40 348 24 216 104 767 308 245
Cuts at: 2402 3547 4305 4882 5378 350
Size: 1145 758 577 496 1094
Fragments arranged by size:
1145 1094 767 758 577 496 348 308
245 216 104 40 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1123 2791 1123
Size: 1668 4454
BsiEI CG_ry'CG
Cuts at: 104 348 1590 3664 3710 4918 104
Size: 244 1242 2074 46 1208 1308
Fragments arranged by size:
2074 1308 1242 1208 244 46
BsiHKAI G_wGCw'C
Cuts at: 752 1361 3116 3136 3914 4104 4896 752
Size: 609 1755 20 778 190 792 1978
Fragments arranged by size:
1978 1755 792 778 609 190 20
BsmI GAATG_Cn'
Cuts at: 694 694
Size: 6122
Bsp1286I G_dGCh'C
Cuts at: 500 682 752 1199 1361 1488 1648 2257 2578
Size: 182 70 447 162 127 160 609 321
Cuts at: 2578 3032 3116 3136 3747 3840 3914 4104 4166
Size: 454 84 20 611 93 74 190 62
Cuts at: 4166 4896 500
Size: 730 1726
Fragments arranged by size:
1726 730 611 609 454 447 321 190
182 162 160 127 93 84 74 70
62 20
BspEI T'CCGG_A
Cuts at: 5342 5342
Size: 6122
BspHI T'CATG_A
Cuts at: 5298 5298
Size: 6122
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1716 1979 2978 3172 3386 3688 4069 1716
Size: 263 999 194 214 302 381 3769
Fragments arranged by size:
3769 999 381 302 263 214 194
BsrBI CCG'CTC
Cuts at: 50 207 2220 3060 4414 4468 5952 6117 50
Size: 157 2013 840 1354 54 1484 165 55
Fragments arranged by size:
2013 1484 1354 840 165 157 55 54
BsrDI GCAATG_nn'
Cuts at: 806 4034 806
Size: 3228 2894
BsrFI r'CCGG_y
Cuts at: 128 793 1690 1984 4120 4301 4510 128
Size: 665 897 294 2136 181 209 1740
Fragments arranged by size:
2136 1740 897 665 294 209 181
BsrGI T'GTAC_A
Cuts at: 2243 2243
Size: 6122
BssSI C'ACGA_G
Cuts at: 4393 4751 4393
Size: 358 5764
BstAPI GCAn_nnn'nTGC
Cuts at: 2133 3499 2133
Size: 1366 4756
BstBI TT'CG_AA
Cuts at: 4483 4483
Size: 6122
BstEII G'GTnAC_C
Cuts at: 906 906
Size: 6122
BstXI CCAn_nnnn'nTGG
Cuts at: 726 2194 2322 726
Size: 1468 128 4526
Fragments arranged by size:
4526 1468 128
BstYI r'GATC_y
Cuts at: 1 434 2778 3147 3614 3637 3972 4218 5230
Size: 433 2344 369 467 23 335 246 1012
Cuts at: 5230 5316 5328 5674 1
Size: 86 12 346 449
Fragments arranged by size:
2344 1012 467 449 433 369 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 2939 2939
Size: 6122
Bsu36I CC'TnA_GG
Cuts at: 565 1006 1058 1874 3024 565
Size: 441 52 816 1150 3663
Fragments arranged by size:
3663 1150 816 441 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4072 4253 4416 4072
Size: 181 163 5778
Fragments arranged by size:
5778 181 163
BtsI GCAGTG_nn'
Cuts at: 421 1049 1979 2052 2209 3365 421
Size: 628 930 73 157 1156 3178
Fragments arranged by size:
3178 1156 930 628 157 73
ClaI AT'CG_AT
Cuts at: 2039 2039
Size: 6122
DraI TTT'AAA
Cuts at: 3121 3121
Size: 6122
DraIII CAC_nnn'GTG
Cuts at: 1365 1365
Size: 6122
DrdI GACnn_nn'nnGTC
Cuts at: 3828 4686 3828
Size: 858 5264
EaeI y'GGCC_r
Cuts at: 2049 3707 3881 4272 4299 5644 2049
Size: 1658 174 391 27 1345 2527
Fragments arranged by size:
2527 1658 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3707 3707
Size: 6122
EarI CTCTTCn'nnn_
Cuts at: 616 2495 4145 4355 616
Size: 1879 1650 210 2383
Fragments arranged by size:
2383 1879 1650 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 520 596 4330 4640 4786 5932 520
Size: 76 3734 310 146 1146 710
Fragments arranged by size:
3734 1146 710 310 146 76
EcoICRI GAG'CTC
Cuts at: 3114 3134 3114
Size: 20 6102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 321 877 982 1015 1108 1120 1165 1207 1264
Size: 556 105 33 93 12 45 42 57
Cuts at: 1264 1274 1510 1618 1667 2236 2519 2548 2932
Size: 10 236 108 49 569 283 29 384
Cuts at: 2932 3947 4379 5126 5394 321
Size: 1015 432 747 268 1049
Fragments arranged by size:
1049 1015 747 569 556 432 384 283
268 236 108 105 93 57 49 45
42 33 29 12 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2974 3052 2974
Size: 78 6044
EcoO109I rG'GnC_Cy
Cuts at: 251 496 497 679 766 1209 1332 2161 2253
Size: 245 1 182 87 443 123 829 92
Cuts at: 2253 2254 2847 3028 3029 3075 3261 251
Size: 1 593 181 1 46 186 3112
Fragments arranged by size:
3112 829 593 443 245 186 182 181
123 92 87 46 1 1 1
EcoRI G'AATT_C
Cuts at: 2707 3126 2707
Size: 419 5703
EcoRV GAT'ATC
Cuts at: 1170 1170
Size: 6122
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 261 738 1407 1461 4141 261
Size: 477 669 54 2680 2242
Fragments arranged by size:
2680 2242 669 477 54
FspI TGC'GCA
Cuts at: 3903 3903
Size: 6122
HaeII r_GCGC'y
Cuts at: 979 1506 3556 3804 4826 5805 979
Size: 527 2050 248 1022 979 1296
Fragments arranged by size:
2050 1296 1022 979 527 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 285 317 1174 1206 3964 3994 3996 4026 285
Size: 32 857 32 2758 30 2 30 2381
Fragments arranged by size:
2758 2381 857 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3161 3161
Size: 6122
KasI G'GCGC_C
Cuts at: 3552 3800 3552
Size: 248 5874
KpnI G_GTAC'C
Cuts at: 1873 3142 1873
Size: 1269 4853
MluI A'CGCG_T
Cuts at: 5555 5555
Size: 6122
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1799 4793 4977 1799
Size: 2994 184 2944
Fragments arranged by size:
2994 2944 184
MscI TGG'CCA
Cuts at: 2051 3883 2051
Size: 1832 4290
MslI CAynn'nnrTG
Cuts at: 1708 1864 2860 3038 4111 4238 1708
Size: 156 996 178 1073 127 3592
Fragments arranged by size:
3592 1073 996 178 156 127
MspA1I CmG'CkG
Cuts at: 125 350 855 1014 1086 1467 1554 1617 2130
Size: 225 505 159 72 381 87 63 513
Cuts at: 2130 2157 2402 2775 3233 3547 3907 4920 5165
Size: 27 245 373 458 314 360 1013 245
Cuts at: 5165 125
Size: 1082
Fragments arranged by size:
1082 1013 513 505 458 381 373 360
314 245 245 225 159 87 72 63
27
NaeI GCC'GGC
Cuts at: 130 795 1986 4303 130
Size: 665 1191 2317 1949
Fragments arranged by size:
2317 1949 1191 665
NarI GG'CG_CC
Cuts at: 3553 3801 3553
Size: 248 5874
NgoMIV G'CCGG_C
Cuts at: 128 793 1984 4301 128
Size: 665 1191 2317 1949
Fragments arranged by size:
2317 1949 1191 665
NheI G'CTAG_C
Cuts at: 426 426
Size: 6122
NruI TCG'CGA
Cuts at: 203 203
Size: 6122
NspI r_CATG'y
Cuts at: 3175 3675 4582 3175
Size: 500 907 4715
Fragments arranged by size:
4715 907 500
PciI A'CATG_T
Cuts at: 4578 4578
Size: 6122
PflMI CCAn_nnn'nTGG
Cuts at: 1205 1758 2058 1205
Size: 553 300 5269
Fragments arranged by size:
5269 553 300
PfoI T'CCnGG_A
Cuts at: 137 1251 2118 2321 2909 137
Size: 1114 867 203 588 3350
Fragments arranged by size:
3350 1114 867 588 203
PmeI GTTT'AAAC
Cuts at: 3121 3121
Size: 6122
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5261 5293 5261
Size: 32 6090
PpuMI rG'GwC_Cy
Cuts at: 251 766 1209 1332 251
Size: 515 443 123 5041
Fragments arranged by size:
5041 515 443 123
PshAI GACnn'nnGTC
Cuts at: 325 1592 325
Size: 1267 4855
PsiI TTA'TAA
Cuts at: 224 224
Size: 6122
PspOMI G'GGCC_C
Cuts at: 496 678 2253 3028 496
Size: 182 1575 775 3590
Fragments arranged by size:
3590 1575 775 182
PstI C_TGCA'G
Cuts at: 1157 1622 1949 2363 3169 3854 1157
Size: 465 327 414 806 685 3425
Fragments arranged by size:
3425 806 685 465 414 327
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6122
PvuII CAG'CTG
Cuts at: 125 855 1014 1086 1617 2130 2157 2775 3547
Size: 730 159 72 531 513 27 618 772
Cuts at: 3547 3907 125
Size: 360 2340
Fragments arranged by size:
2340 772 730 618 531 513 360 159
72 27
RsrII CG'GwC_CG
Cuts at: 4317 4317
Size: 6122
SacI G_AGCT'C
Cuts at: 3116 3136 3116
Size: 20 6102
SalI G'TCGA_C
Cuts at: 3159 3159
Size: 6122
SanDI GG'GwC_CC
Cuts at: 251 1209 251
Size: 958 5164
SapI GCTCTTCn'nnn_
Cuts at: 4145 4355 4145
Size: 210 5912
SbfI CC_TGCA'GG
Cuts at: 3169 3169
Size: 6122
ScaI AGT'ACT
Cuts at: 5639 5639
Size: 6122
SfcI C'TryA_G
Cuts at: 33 1153 1618 1945 2081 2301 2359 3165 3850
Size: 1120 465 327 136 220 58 806 685
Cuts at: 3850 4843 5034 33
Size: 993 191 1121
Fragments arranged by size:
1121 1120 993 806 685 465 327 220
191 136 58
SfoI GGC'GCC
Cuts at: 3554 3802 3554
Size: 248 5874
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6122
SmaI CCC'GGG
Cuts at: 2839 3144 2839
Size: 305 5817
SmlI C'TyrA_G
Cuts at: 1576 2803 3277 4192 4684 4946 1576
Size: 1227 474 915 492 262 2752
Fragments arranged by size:
2752 1227 915 492 474 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6122
SphI G_CATG'C
Cuts at: 3175 3675 3175
Size: 500 5622
StuI AGG'CCT
Cuts at: 2365 2365
Size: 6122
StyI C'CwwG_G
Cuts at: 2712 2843 3256 3610 2712
Size: 131 413 354 5224
Fragments arranged by size:
5224 413 354 131
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3705 3838 4297 4506 3705
Size: 133 459 209 5321
Fragments arranged by size:
5321 459 209 133
TatI w'GTAC_w
Cuts at: 2243 5637 2243
Size: 3394 2728
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 459 491 887 919 1638 1670 3085 3117 5639
Size: 32 396 32 719 32 1415 32 2522
Cuts at: 5639 5671 459
Size: 32 910
Fragments arranged by size:
2522 1415 910 719 396 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1378 3919 1378
Size: 2541 3581
XbaI T'CTAG_A
Cuts at: 3153 3153
Size: 6122
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3617 3617
Size: 6122
XmaI C'CCGG_G
Cuts at: 2837 3142 2837
Size: 305 5817
XmnI GAAnn'nnTTC
Cuts at: 2905 2905
Size: 6122
ZraI GAC'GTC
Cuts at: 1548 1548
Size: 6122
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgZI BtsI ClaI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NheI NruI NspI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI
BssHII BtgI CspCI FseI FspAI HindIII HpaI MfeI
NcoI NdeI NotI NsiI PacI PasI PmlI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI