################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## AatII AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I AspEI AspI AvaI AviII AvrII BalI BbeI BbrPI BbsI BbvCI BciVI BclI BfrI BglI BglII BlnI BpuAI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsoBI BspEI BspLU11I BspMI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV FbaI FseI FspI HincII HindII HpaI KasI MaeII MamI MfeI MluI MluNI MroI MscI MunI NarI NcoI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI StuI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 262 GGTACC G^GTACC AccI 1454 GTAGAC GT^[AC][GT]AC AcsI 1502 AAATTC [GA]^AATT[CT] AcyI 89 GGCGTC G[GA]^CG[CT]C ApaI 573 GGGCCC GGGCC^C ApoI 1502 AAATTC [GA]^AATT[CT] Asp718I 262 GGTACC G^GTACC AspHI 769 GTGCTC G[AT]GC[AT]^C BamHI 851 GGATCC G^GATCC BanI 262 GGTACC G^G[CT][GA]CC BcgI 400 GCAGTGGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 645 GCTGAGC GC^T[ACGT]AGC Bpu1102I 645 GCTGAGC GC^T[ACGT]AGC BsaHI 89 GGCGTC G[GA]^CG[CT]C BsiHKAI 769 GTGCTC G[AT]GC[AT]^C BsmAI 1413 GTCTC GTCTC(1/5) BsmI 668 GAATGC GAATGC(1/-1) BspDI 1477 ATCGAT AT^CGAT BspHI 1083 TCATGA T^CATGA BsrBI 723 GAGCGG CCGCTC(-3/-3) BsrDI 290 GCAATG GCAATG(2/0) BsrI 35 ACTGG ACTGG(1/-1) BstAPI 406 GCATCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 645 GCTGAGC GC^T[ACGT]AGC ClaI 1477 ATCGAT AT^CGAT DraIII 123 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 870 GACCCTGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1380 GAAGAG CTCTTC(1/4) EcoNI 1054 CCTGAATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 941 ATGCAT ATGCA^T Hin1I 89 GGCGTC G[GA]^CG[CT]C HindIII 1471 AAGCTT A^AGCTT KpnI 262 GGTACC GGTAC^C Ksp632I 1380 GAAGAG CTCTTC(1/4) KspI 61 CCGCGG CCGC^GG MaeIII 1119 GTTAC ^GT[ACGT]AC MslI 1078 CATCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NsiI 941 ATGCAT ATGCA^T NspI 396 GCATGC [GA]CATG^[CT] RcaI 1083 TCATGA T^CATGA SacII 61 CCGCGG CCGC^GG SapI 1380 GAAGAGC GCTCTTC(1/4) SexAI 512 ACCTGGT A^CC[AT]GGT SmlI 802 CTCAAG C^T[CT][GA]AG SphI 396 GCATGC GCATG^C TaqI 1478 TCGA T^CGA TthHB8I 1478 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccII 62 CGCG CG^CG 220 CGCG BsmFI 58 GTCCC GGGAC(10/14) 282 GTCCC Bsp1407I 320 TGTACA T^GTACA 1185 TGTACA BsrFI 86 GCCGGC [GA]^CCGG[CT] 351 GCCGGC BsrGI 320 TGTACA T^GTACA 1185 TGTACA BstUI 62 CGCG CG^CG 220 CGCG BstXI 134 CCATCTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 285 CCAGGGCAATGG BstYI 617 GGATCT [GA]^GATC[CT] 851 GGATCC Cfr10I 86 GCCGGC [GA]^CCGG[CT] 351 GCCGGC DpnII 618 GATC ^GATC 852 GATC DsaI 61 CCGCGG C^C[GA][CT]GG 363 CCGTGG HaeII 631 AGCGCC [GA]GCGC^[CT] 763 GGCGCT HgaI 56 GCGTC GACGC(5/10) 90 GCGTC MboI 618 GATC ^GATC 852 GATC MflI 617 GGATCT [GA]^GATC[CT] 851 GGATCC MspA1I 61 CCGCGG C[AC]G^C[GT]G 774 CCGCTG MvnI 62 CGCG CG^CG 220 CGCG NaeI 86 GCCGGC GCC^GGC 351 GCCGGC NdeII 618 GATC ^GATC 852 GATC NgoMIV 86 GCCGGC G^CCGGC 351 GCCGGC PleI 674 GACTC GAGTC(4/5) 280 GAGTC PpuMI 97 AGGACCC [GA]G^G[AT]CC[CT] 868 AGGACCC Sau3AI 618 GATC ^GATC 852 GATC SspBI 320 TGTACA T^GTACA 1185 TGTACA TfiI 1057 GAATC G^A[AT]TC 1157 GATTC XhoII 617 GGATCT [GA]^GATC[CT] 851 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AvaII BanII BbvI BcnI BfaI BmyI BpmI BsaJI BsiYI BslI Bsp1286I BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DraII EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MboII MnlI MseI MspI MvaI MwoI NciI NlaIII NlaIV PstI RsaI Sau96I ScrFI SfaNI SfcI StyI Tru9I TseI Tsp509I TspRI