(Circular) (Six-base) MAPSORT of: pF1KE0308.seq Check: 8203 from: 1 to: 4670 pF1KE0308 4670 bp With 182 enzymes: SgfI * April 24, 2009 13:12 .. AccI GT'mk_AC Cuts at: 1563 1708 1563 Size: 145 4525 Acc65I G'GTAC_C Cuts at: 370 1686 370 Size: 1316 3354 AclI AA'CG_TT Cuts at: 4510 4510 Size: 4670 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 541 1046 1501 2495 2927 3674 3942 541 Size: 505 455 994 432 747 268 1269 Fragments arranged by size: 1269 994 747 505 455 432 268 AflIII A'CryG_T Cuts at: 2022 3126 4103 2022 Size: 1104 977 2589 Fragments arranged by size: 2589 1104 977 AgeI A'CCGG_T Cuts at: 3058 3058 Size: 4670 AlwNI CAG_nnn'CTG Cuts at: 1122 1471 1480 1524 1975 3542 1122 Size: 349 9 44 451 1567 2250 Fragments arranged by size: 2250 1567 451 349 44 9 ApaI G_GGCC'C Cuts at: 685 685 Size: 4670 ApaLI G'TGCA_C Cuts at: 3440 3440 Size: 4670 ApoI r'AATT_y Cuts at: 1610 1674 4023 4279 1610 Size: 64 2349 256 2001 Fragments arranged by size: 2349 2001 256 64 AseI AT'TA_AT Cuts at: 20 20 Size: 4670 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4670 AvaI C'yCGr_G Cuts at: 1690 1690 Size: 4670 BamHI G'GATC_C Cuts at: 959 1695 959 Size: 736 3934 BanI G'GyrC_C Cuts at: 370 1686 2100 2348 2383 370 Size: 1316 414 248 35 2657 Fragments arranged by size: 2657 1316 414 248 35 BanII G_rGCy'C Cuts at: 685 812 840 971 1684 2714 685 Size: 127 28 131 713 1030 2641 Fragments arranged by size: 2641 1030 713 131 127 28 BbeI G_GCGC'C Cuts at: 2104 2352 2104 Size: 248 4422 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 497 531 497 Size: 34 4636 BciVI GTATCCnnnnn_n' Cuts at: 2568 3329 4670 2568 Size: 761 1341 2568 Fragments arranged by size: 2568 1341 761 BclI T'GATC_A Cuts at: 2190 2190 Size: 4670 BfrBI ATG'CAT Cuts at: 1051 1051 Size: 4670 BglII A'GATC_T Cuts at: 2185 4222 2185 Size: 2037 2633 BlpI GC'TnA_GC Cuts at: 754 1782 754 Size: 1028 3642 Bme1580I G_kGCm'C Cuts at: 232 685 2295 2388 3444 232 Size: 453 1610 93 1056 1458 Fragments arranged by size: 1610 1458 1056 453 93 BmrI ACTGGGnnnn_n' Cuts at: 1882 1963 2298 4442 1882 Size: 81 335 2144 2110 Fragments arranged by size: 2144 2110 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 299 3037 299 Size: 2738 1932 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 207 271 368 666 690 874 207 Size: 64 97 298 24 184 4003 Fragments arranged by size: 4003 298 184 97 64 24 Bpu10I CC'TnA_GC Cuts at: 608 608 Size: 4670 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 895 1846 2725 3217 3515 895 Size: 951 879 492 298 2050 Fragments arranged by size: 2050 951 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 4374 4374 Size: 4670 BsaAI yAC'GTr Cuts at: 2023 2023 Size: 4670 BsaBI GATnn'nnATC Cuts at: 6 2184 4221 6 Size: 2178 2037 455 Fragments arranged by size: 2178 2037 455 BsaHI Gr'CG_yC Cuts at: 198 2101 2349 3051 4547 198 Size: 1903 248 702 1496 321 Fragments arranged by size: 1903 1496 702 321 248 BsaWI w'CCGG_w Cuts at: 1859 2380 3058 3332 3479 3890 1859 Size: 521 678 274 147 411 2639 Fragments arranged by size: 2639 678 521 411 274 147 BseYI C'CCAG_C Cuts at: 225 700 2095 2853 3430 3926 225 Size: 475 1395 758 577 496 969 Fragments arranged by size: 1395 969 758 577 496 475 BsiEI CG_ry'CG Cuts at: 104 2212 2258 3466 104 Size: 2108 46 1208 1308 Fragments arranged by size: 2108 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 881 1684 2462 2652 3444 881 Size: 803 778 190 792 2107 Fragments arranged by size: 2107 803 792 778 190 BsmI GAATG_Cn' Cuts at: 782 782 Size: 4670 Bsp1286I G_dGCh'C Cuts at: 232 685 812 840 881 971 1684 2295 2388 Size: 453 127 28 41 90 713 611 93 Cuts at: 2388 2462 2652 2714 3444 232 Size: 74 190 62 730 1458 Fragments arranged by size: 1458 730 713 611 453 190 127 93 90 74 62 41 28 BspEI T'CCGG_A Cuts at: 3890 3890 Size: 4670 BspHI T'CATG_A Cuts at: 1191 3846 1191 Size: 2655 2015 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1720 1934 2236 2617 1720 Size: 214 302 381 3773 Fragments arranged by size: 3773 381 302 214 BsrBI CCG'CTC Cuts at: 50 833 2962 3016 4500 4665 50 Size: 783 2129 54 1484 165 55 Fragments arranged by size: 2129 1484 783 165 55 54 BsrDI GCAATG_nn' Cuts at: 405 2582 405 Size: 2177 2493 BsrFI r'CCGG_y Cuts at: 194 459 2668 2849 3058 194 Size: 265 2209 181 209 1806 Fragments arranged by size: 2209 1806 265 209 181 BsrGI T'GTAC_A Cuts at: 428 1293 428 Size: 865 3805 BssSI C'ACGA_G Cuts at: 2941 3299 2941 Size: 358 4312 BstAPI GCAn_nnn'nTGC Cuts at: 520 2047 520 Size: 1527 3143 BstBI TT'CG_AA Cuts at: 3031 3031 Size: 4670 BstXI CCAn_nnnn'nTGG Cuts at: 249 400 249 Size: 151 4519 BstYI r'GATC_y Cuts at: 1 725 959 1695 2162 2185 2520 2766 3778 Size: 724 234 736 467 23 335 246 1012 Cuts at: 3778 3864 3876 4222 1 Size: 86 12 346 449 Fragments arranged by size: 1012 736 724 467 449 346 335 246 234 86 23 12 BtgI C'CryG_G Cuts at: 169 471 169 Size: 302 4368 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 304 2620 2801 2964 304 Size: 2316 181 163 2010 Fragments arranged by size: 2316 2010 181 163 BtsI GCAGTG_nn' Cuts at: 230 515 806 1913 230 Size: 285 291 1107 2987 Fragments arranged by size: 2987 1107 291 285 ClaI AT'CG_AT Cuts at: 1586 1586 Size: 4670 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 453 488 453 Size: 35 4635 DraI TTT'AAA Cuts at: 1669 1669 Size: 4670 DraIII CAC_nnn'GTG Cuts at: 236 236 Size: 4670 DrdI GACnn_nn'nnGTC Cuts at: 984 2376 3234 984 Size: 1392 858 2420 Fragments arranged by size: 2420 1392 858 EaeI y'GGCC_r Cuts at: 461 1020 1146 2255 2429 2820 2847 4192 461 Size: 559 126 1109 174 391 27 1345 939 Fragments arranged by size: 1345 1109 939 559 391 174 126 27 EagI C'GGCC_G Cuts at: 2255 2255 Size: 4670 EarI CTCTTCn'nnn_ Cuts at: 1483 2693 2903 1483 Size: 1210 210 3250 Fragments arranged by size: 3250 1210 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 805 2878 3188 3334 4480 805 Size: 2073 310 146 1146 995 Fragments arranged by size: 2073 1146 995 310 146 EcoICRI GAG'CTC Cuts at: 1682 1682 Size: 4670 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 207 271 368 541 666 690 874 1046 1501 Size: 64 97 173 125 24 184 172 455 Cuts at: 1501 2495 2927 3674 3942 207 Size: 994 432 747 268 935 Fragments arranged by size: 994 935 747 455 432 268 184 173 172 125 97 64 24 EcoNI CCTnn'n_nnAGG Cuts at: 1166 1166 Size: 4670 EcoO109I rG'GnC_Cy Cuts at: 206 278 681 977 1809 206 Size: 72 403 296 832 3067 Fragments arranged by size: 3067 832 403 296 72 EcoRI G'AATT_C Cuts at: 1674 1674 Size: 4670 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 936 1422 2689 936 Size: 486 1267 2917 Fragments arranged by size: 2917 1267 486 FspI TGC'GCA Cuts at: 2451 2451 Size: 4670 HaeII r_GCGC'y Cuts at: 743 875 2104 2352 3374 4353 743 Size: 132 1229 248 1022 979 1060 Fragments arranged by size: 1229 1060 1022 979 248 132 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1224 1256 2512 2542 2544 2574 1224 Size: 32 1256 30 2 30 3320 Fragments arranged by size: 3320 1256 32 30 30 2 HincII GTy'rAC Cuts at: 1709 1709 Size: 4670 HindIII A'AGCT_T Cuts at: 1579 1579 Size: 4670 KasI G'GCGC_C Cuts at: 2100 2348 2100 Size: 248 4422 KpnI G_GTAC'C Cuts at: 374 1690 374 Size: 1316 3354 MluI A'CGCG_T Cuts at: 4103 4103 Size: 4670 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 525 3341 3525 525 Size: 2816 184 1670 Fragments arranged by size: 2816 1670 184 MscI TGG'CCA Cuts at: 2431 2431 Size: 4670 MslI CAynn'nnrTG Cuts at: 1190 2659 2786 1190 Size: 1469 127 3074 Fragments arranged by size: 3074 1469 127 MspA1I CmG'CkG Cuts at: 171 884 1781 2095 2455 3468 3713 171 Size: 713 897 314 360 1013 245 1128 Fragments arranged by size: 1128 1013 897 713 360 314 245 NaeI GCC'GGC Cuts at: 196 461 2851 196 Size: 265 2390 2015 Fragments arranged by size: 2390 2015 265 NarI GG'CG_CC Cuts at: 2101 2349 2101 Size: 248 4422 NgoMIV G'CCGG_C Cuts at: 194 459 2849 194 Size: 265 2390 2015 Fragments arranged by size: 2390 2015 265 NsiI A_TGCA'T Cuts at: 1053 1053 Size: 4670 NspI r_CATG'y Cuts at: 508 1723 2223 3130 508 Size: 1215 500 907 2048 Fragments arranged by size: 2048 1215 907 500 PasI CC'CwG_GG Cuts at: 393 526 393 Size: 133 4537 PciI A'CATG_T Cuts at: 3126 3126 Size: 4670 PfoI T'CCnGG_A Cuts at: 663 663 Size: 4670 PmeI GTTT'AAAC Cuts at: 1669 1669 Size: 4670 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3809 3841 3809 Size: 32 4638 PpuMI rG'GwC_Cy Cuts at: 206 977 206 Size: 771 3899 PsiI TTA'TAA Cuts at: 1238 1238 Size: 4670 PspOMI G'GGCC_C Cuts at: 681 681 Size: 4670 PstI C_TGCA'G Cuts at: 416 698 1476 1717 2402 416 Size: 282 778 241 685 2684 Fragments arranged by size: 2684 778 685 282 241 PvuI CG_AT'CG Cuts at: 104 104 Size: 4670 PvuII CAG'CTG Cuts at: 2095 2455 2095 Size: 360 4310 RsrII CG'GwC_CG Cuts at: 2865 2865 Size: 4670 SacI G_AGCT'C Cuts at: 1684 1684 Size: 4670 SacII CC_GC'GG Cuts at: 172 172 Size: 4670 SalI G'TCGA_C Cuts at: 1707 1707 Size: 4670 SapI GCTCTTCn'nnn_ Cuts at: 1483 2693 2903 1483 Size: 1210 210 3250 Fragments arranged by size: 3250 1210 210 SbfI CC_TGCA'GG Cuts at: 1717 1717 Size: 4670 ScaI AGT'ACT Cuts at: 4187 4187 Size: 4670 SexAI A'CCwGG_T Cuts at: 620 620 Size: 4670 SfcI C'TryA_G Cuts at: 33 412 590 694 729 1472 1713 2398 3391 Size: 379 178 104 35 743 241 685 993 Cuts at: 3391 3582 33 Size: 191 1121 Fragments arranged by size: 1121 993 743 685 379 241 191 178 104 35 SfoI GGC'GCC Cuts at: 2102 2350 2102 Size: 248 4422 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4670 SmaI CCC'GGG Cuts at: 1692 1692 Size: 4670 SmlI C'TyrA_G Cuts at: 910 1825 2740 3232 3494 910 Size: 915 915 492 262 2086 Fragments arranged by size: 2086 915 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4670 SphI G_CATG'C Cuts at: 508 1723 2223 508 Size: 1215 500 2955 Fragments arranged by size: 2955 1215 500 StyI C'CwwG_G Cuts at: 202 920 1326 1372 1804 2158 202 Size: 718 406 46 432 354 2714 Fragments arranged by size: 2714 718 432 406 354 46 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1093 2253 2386 2845 3054 1093 Size: 1160 133 459 209 2709 Fragments arranged by size: 2709 1160 459 209 133 TatI w'GTAC_w Cuts at: 428 1174 1293 4185 428 Size: 746 119 2892 913 Fragments arranged by size: 2892 913 746 119 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1337 1369 4187 4219 1337 Size: 32 2818 32 1788 Fragments arranged by size: 2818 1788 32 32 Tth111I GACn'n_nGTC Cuts at: 2467 2467 Size: 4670 XbaI T'CTAG_A Cuts at: 1701 1701 Size: 4670 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2165 2165 Size: 4670 XmaI C'CCGG_G Cuts at: 1690 1690 Size: 4670 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI PasI PciI PfoI PmeI PpiI PpuMI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI AscI AvrII BaeI BbsI BbvCI BglI BmgBI BmtI BsaXI BseRI BsgI BsiWI BsmBI BssHII BstEII BstZ17I Bsu36I EcoRV FseI FspAI HpaI MfeI NcoI NdeI NheI NotI NruI PacI PflMI PmlI PshAI PspXI PsrI SanDI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI