Restriction map for pF1KE0295
##################################
# NO CUT ENZYMES (  147 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
AlwI        AlwNI       AocI        Aor51HI     ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AviII       AvrII       BalI        BamHI       
BbrPI       BbvCI       BciVI       BclI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrDI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstF5I      BstPI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DrdI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FokI        FseI        FspI        HincII      HindII      
HindIII     HpaI        KpnI        KspI        MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MseI        MslI        MspA1I      MunI        
NcoI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflMI       
PinAI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        TfiI        Tru9I       
Tsp45I      Tsp509I     Van91I      XbaI        XhoI        
XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AfaI          675  GTAC                 GT^AC
Alw44I        580  GTGCAC               G^TGCAC
ApaLI         580  GTGCAC               G^TGCAC
AspI          421  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbsI           31  GTCTTC               GAAGAC(2/6)
BcgI          138  CGAGTCTGGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          151  CTAG                 C^TAG
BpuAI          31  GTCTTC               GAAGAC(2/6)
BsaI           22  GAGACC               GGTCTC(1/5)
BseRI         111  CTCCTC               GAGGAG(10/8)
BsgI          604  CTGCAC               GTGCAG(16/14)
BsmAI          22  GAGAC                GTCTC(1/5)
BsrBI         108  CCGCTC               CCGCTC(-3/-3)
BstAPI        582  GCACACCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII        180  CACGCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI          523  CTCTTC               CTCTTC(1/4)
EcoT14I       215  CCAAGG               C^C[AT][AT]GG
HgaI          297  GACGC                GACGC(5/10)
HinfI         139  GAGTC                G^A[ACGT]TC
HphI          230  TCACC                GGTGA(8/7)
Ksp632I       523  CTCTTC               CTCTTC(1/4)
MaeI          151  CTAG                 C^TAG
MaeII         578  ACGT                 A^CGT
MaeIII        506  GTTAC                ^GT[ACGT]AC
NaeI          444  GCCGGC               GCC^GGC
NgoMIV        444  GCCGGC               G^CCGGC
NspI          188  GCATGT               [GA]CATG^[CT]
PflFI         421  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          139  GAGTC                GAGTC(4/5)
PpuMI         164  GGGTCCT              [GA]G^G[AT]CC[CT]
PshAI          24  GACCTTTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          301  CTGCAG               CTGCA^G
RsaI          675  GTAC                 GT^AC
SfaNI         260  GATGC                GCATC(5/9)
SfcI          301  CTGCAG               C^T[GA][CT]AG
StuI          305  AGGCCT               AGG^CCT
StyI          215  CCAAGG               C^C[AT][AT]GG
TaqI          665  TCGA                 T^CGA
Tth111I       421  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       665  TCGA                 T^CGA
XcmI          332  CCATCTGCCTTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AcyI          283  GGCGCC               G[GA]^CG[CT]C
              395  GGCGCC              
AspHI         130  GAGCAC               G[AT]GC[AT]^C
              580  GTGCAC              
BbeI          283  GGCGCC               GGCGC^C
              395  GGCGCC              
BpmI          118  CTGGAG               CTGGAG(16/14)
              700  CTGGAG              
BsaHI         283  GGCGCC               G[GA]^CG[CT]C
              395  GGCGCC              
BsiHKAI       130  GAGCAC               G[AT]GC[AT]^C
              580  GTGCAC              
BsmFI         419  GGGAC                GGGAC(10/14)
               90  GTCCC               
BsrFI         232  ACCGGC               [GA]^CCGG[CT]
              444  GCCGGC              
BsrI           52  ACTGG                ACTGG(1/-1)
               62  CCAGT               
Cfr10I        232  ACCGGC               [GA]^CCGG[CT]
              444  GCCGGC              
DpnII         228  GATC                 ^GATC
              597  GATC                
FauI          407  CCCGC                CCCGC(4/6)
              453  GCGGG               
Hin1I         283  GGCGCC               G[GA]^CG[CT]C
              395  GGCGCC              
KasI          283  GGCGCC               G^GCGCC
              395  GGCGCC              
MboI          228  GATC                 ^GATC
              597  GATC                
NarI          283  GGCGCC               GG^CGCC
              395  GGCGCC              
NdeII         228  GATC                 ^GATC
              597  GATC                
NlaIII        189  CATG                 CATG^
              672  CATG                
Sau3AI        228  GATC                 ^GATC
              597  GATC                
SfoI          283  GGCGCC               GGC^GCC
              395  GGCGCC              


#########################################
# 3 OR MORE CUT ENZYMES (   50 enzymes) #
#########################################

AccII       AciI        AluI        ApaI        AvaI        
AvaII       BanI        BanII       BbvI        BcnI        
BmyI        BsaJI       BsiYI       BslI        BsoBI       
Bsp1286I    BssKI       BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DraII       DsaI        
EcoO109I    EcoRII      Fnu4HI      HaeII       HaeIII      
HapII       HhaI        HinP1I      HpaII       ItaI        
MboII       MnlI        MspI        MvaI        MvnI        
MwoI        NciI        NlaIV       Sau96I      ScrFI       
SmaI        TseI        TspRI       XmaCI       XmaI        
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